LOCUS       RR208311                6612 bp    DNA     circular  SYN 
DEFINITION  Thbd (Myc-DDK-tagged ORF) - Rat thrombomodulin (Thbd), (10 ug)
ACCESSION   NM_031771
VERSION     NM_031771.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2769..2860 
                     /label="Myc-DDK"  
     misc_feature    2890..3475 
                     /label="PolyA signal"  
     misc_feature    2947..2966 
                     /label="Restriction Enzyme"  
     misc_feature    3751..4423 
                     /label="ColE1"  
     misc_feature    4853..5647 
                     /label="Kan/Neo"  
     misc_feature    5682..5984 
                     /label="SV40 ori"  
     misc_feature    6070..6547 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2751..2757 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2751 
                     /label="Thbd"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTTGGGGTT TTCCTTCTGG GTGTGCTGGC TCCAGCTGGC CTGGGGATCT 
     1081 CTGCACTAGC CAAGCTGCAG CCCAAAGGCA GTCAATGCGT GGGGAATGAG TGCTTCGCGC 
     1141 TTTTCCAGGA CCCCGTGACC TTCCTCGATG CCAGCCAGGC CTGCCAGCGC CTGCAGGGAC 
     1201 ATTTGATGAC AGTGCGCTCC TCGGTGGCTG CGGATGTCAT CTCCCTTCTG GTGAGCGACA 
     1261 GTAGTATGGA CTCACGGCCC TGGATCGGTT TACAGCTCCC TCAGGGCTGT GGTGACCCGG 
     1321 TGCATCTCGG GCCCCTGCGC GGCTTCCAGT GGGTTACTGG GGATAACCAC ACCAGTTACA 
     1381 GCAGGTGGGC GCGGCCCAAC GACCAGTCGC CTCCACTTTG CGGTCCGCTG TGCGTCACGG 
     1441 TCTCAACAGC AACAGAAGCT GCACCAGGCG AGCCGGCCTG GGAAGAGAAG CCGTGCGAGA 
     1501 ACGAGACCAA GGGTTTTCTC TGCGAGTTTT ACTTCGCAGC TTTCTGCAGG CCTTTACGGG 
     1561 TGAATACCCG AGATCCTGAG GGTGCCCACA TCTCTAGCAC CTACAATACC CCATTGGGGG 
     1621 TCAGCGGCGC GGATTTTCAG ACGCTGCCGA TAGGCAGTTC TGCTACCGTG GCGCCCTTTG 
     1681 GCTTGGAGCT GGTGTGCAGG GCCCTGCCCG GAACTTCAGA GGGACACTGG ACTCGGGAAG 
     1741 TGACAGGAGC TTGGAACTGC AGCGTGGAGA ATGGTGGCTG CGAGTACATG TGCAACAGGA 
     1801 GCGCTAACGG ACCCAGATGC GTCTGCCCCA GCGGCGGGGA CCTGCAGGCT GATGGCCGTT 
     1861 CGTGCGCAAA ACCTGTGGCT CAATTGTGCA ACGAACTCTG CCAGCATTTT TGCGTCAACA 
     1921 ACTCTGATGT GCCAGGCTCT TATTCCTGTA TGTGCGAAAC AGGCTACCAG TTGGCGGCAG 
     1981 ACGGACATCG GTGTGAGGAC GTGGATGACT GTAAGCAGGG GCCCAATCCA TGCCCTCAGC 
     2041 TCTGTTCTAA CACCGAGGGC GGCTTCGAAT GCCGCTGCTA TGATGGCTAT GAGTTGGTGG 
     2101 ACGGAGAGTG CGTGGAGCAA CTGGATCCGT GCTTCAGATC TAAATGCGAG TATCAGTGCC 
     2161 AGCCAGTAAA CTCCACGCAC TACAATTGCA TCTGTGCTGA GGGCTTCGCA CCTAAGCTGG 
     2221 ATGATCCCGA CAGGTGCGAA ATGTTCTGCA ATGAAACTTC GTGCCCAGCA GACTGTGACC 
     2281 CCAACTCCCC AAGCTTTTGT CAATGCCCTG AGGGCTTCAT CCTGGACGAG GGTTCCATAT 
     2341 GCACAGACAT TGATGAGTGC AGTCAAGGCG AATGCCTCAC CAATGAATGT CGAAACCTTC 
     2401 CTGGCTCCTA TGAGTGCATC TGCGGACCTG ACACAGCCCT TGCTGGTCAG ATTAGCAAGG 
     2461 ACTGTGACCC CATCCCTGTT CTGGAGGACT CAGAGGATGG TGGCTCTGGG GAGCACCCAT 
     2521 CAAGCAATCC GACGGTAGTC TCTTCGACAG TTCCCCCTTC TGCAAGACCA ATGCACTCTG 
     2581 GTGTGCTCAT TGGGATCTCC ATTGCCAGCC TGTCCCTGGT GGTGGCGCTT TTGGCGCTTC 
     2641 TTTGTCACCT GCGCAAGAAG CAGGGCACTG CTCGCGCAGA GCTGGAGTAC AAGTGTACCT 
     2701 CTTCAGCCAA GGAGGTAGTA CTGCAGCACG TGAGGACTGA TCGGACGCTG CAGAAGTTCA 
     2761 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     2821 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     2881 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     2941 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3001 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3061 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3121 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3181 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3241 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3301 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     3361 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     3421 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     3481 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     3541 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     3601 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     3661 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     3721 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     3781 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     3841 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     3901 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     3961 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4021 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4081 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4141 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4201 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4261 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4321 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     4381 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     4441 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     4501 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     4561 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     4621 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     4681 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     4741 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     4801 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     4861 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     4921 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     4981 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5041 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5101 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5161 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5221 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5281 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     5341 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     5401 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     5461 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     5521 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     5581 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     5641 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     5701 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     5761 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     5821 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     5881 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     5941 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6001 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6061 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6121 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6181 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6241 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6301 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     6361 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     6421 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     6481 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     6541 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     6601 AACGCGAATT TT
//