LOCUS       RR208299                8880 bp    DNA     circular  SYN 
DEFINITION  Sos2 (Myc-DDK-tagged ORF) - Rat son of sevenless homolog 2 (Drosophila) (Sos2), (10 ug)
ACCESSION   NM_001135561
VERSION     NM_001135561.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5037..5128 
                     /label="Myc-DDK"  
     misc_feature    5158..5743 
                     /label="PolyA signal"  
     misc_feature    5215..5234 
                     /label="Restriction Enzyme"  
     misc_feature    6019..6691 
                     /label="ColE1"  
     misc_feature    7121..7915 
                     /label="Kan/Neo"  
     misc_feature    7950..8252 
                     /label="SV40 ori"  
     misc_feature    8338..8815 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5019..5025 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5019 
                     /label="Sos2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCAGGCG CCGCAGCCCT ACGAGTTCTT CAGCGAAGAG AACAGCCCGA 
     1081 AATGGCGGGG ACTGTTGGTC CCGGCCCTGC GGAAGGTTCA GGAGCAAGTG CACCCCACCC 
     1141 TGTCGGCTAA CGAAGAGTCT CTCTATTACA TCGAAGAACT CATCTTTCAA CTGCTCAATA 
     1201 AGCTGTGCCT GGCTCAACCG AGGACTGTTC AAGATGTTGA GGAACGAGTT CAAAAGACTT 
     1261 TTCCTCATCC TATTGATAAA TGGGCAATTG CTGATGCACA GTCTGCCATA GAGAAACGAA 
     1321 AACGAAGAAA TCCTCTCTTA CTACCTGTGG ACAAAATCCA TCCTTCCTTG AAGGAAGTTT 
     1381 TGGGGTATAA AGTGGACTAC CATGTATCCC TCTACATTGT GGCTGTATTG GAGTATATCT 
     1441 CAGCAGATAT TTTGAAATTG GCTGGTAATT ATGTTTTTAA TATCCGGCAT TATGAAATAT 
     1501 CTCAGCAAGA CATTAAAGTG TCAATGTGTG CAGATAAGGT TTTGATGGAC ATGTTTGACC 
     1561 AGGATGACGA TATAGGCTTG GTTTCTCTCT GTGAAGAGGA ACCTTGTTCT TCTGGTGAGC 
     1621 TAAACTATTA TGACCTTGTT AGAACTGAAA TCGCAGAAGA AAGACAGTAT CTACGGGAAC 
     1681 TGAATATGAT CATTAAAGTA TTCCGGGAAG CCTTTCTTTC AGACAAAAAG TTGTTCAAGT 
     1741 CTTCTGAAAT TGAAAAGATT TTCAGTAACA TTTCAGATAT ACATGAACTG ACTGTGAAAC 
     1801 TTTTAGGTTT GATTGAAGAC ACAGTAGAAA TGACAGATGA AAGCAGTCCT CACCCGCTAG 
     1861 CTGGTAGCTG CTTTGAAGAT TTAGCAGAGG AGCAGGCTTT TGACCCCTAT GAAACGTTAT 
     1921 CACAGGACAT TCTTGCTCCA GAGTTTAATG ACCACTTCAG CAAGTTGATG GCCAGACCTG 
     1981 CAGTTGCTCT GCACTTTCAG TCCATTGCTG ACGGCTTTAA GGAGGCTGTT CGATATGTCC 
     2041 TTCCGCGCCT CATGCTGGTG CCGGTGTATC ATTGTTGGCA TTACTTTGAA TTATTAAAGC 
     2101 AGTTGAAAGC ATGTAGCGAA GAACAGGAAG ACCGAGAGTG TTTGAATCAG GCTATCACTG 
     2161 CCCTCATGAA CCTCCAAGGC AGCATGGACC GGATTTACAA GCAGCACTCG CCAAGGCGCC 
     2221 GGCCAGGGGA TCCTGTTTGC CTTTTTTACA ATCGTCAATT AAGAAGCAAG CACCTGGCTA 
     2281 TCAAAAAAAT GAATGAAATT CAGAAAAACA TTGATGGGTG GGAAGGCAAA GATATTGGAC 
     2341 AGTGTTGTAA TGAGTTCATA ATGGAAGGAC CATTGACCAG AATTGGTGCT AAGCATGAAA 
     2401 GGCATATTTT CCTCTTCGAT GGCTTAATGA TCAGCTGTAA ACCCAATCAT TGCCAGACTC 
     2461 GACTTCCAGG GTATAGTAGT GCAGAATACA GGTTAAAAGA GAAGTTTGTC ATGAGGAAAA 
     2521 TTCAAATCTG TGATAAAGAA GACGCTTGTG AATACAAACA TGCTTTTGAA TTAGTATCCA 
     2581 AAGATGAAAA CAGTGTAATA TTTGCTGCCA AGTCAGCTGA AGAGAAAAAC AACTGGATGG 
     2641 CAGCCCTTAT CTCCCTTCAC TATCGGAGCA CTCTAGATAG AATGCTGGAC TCTGTGTTAC 
     2701 TGAAAGAGGA GAACGAGCAG CCCCTGCGGC TACCGCGGCC AGACGTGTAC CGCTTCGTGG 
     2761 TGACAGACTC AGAGGAGAAC ATAGTTTTTG AAGACAACTT GCAAAGCAGA AGTGGAATCC 
     2821 CCATTATTAA AGGAGGCACC GTGGTGAAAC TGATCGAAAG GCTAACATAT CACATGTATG 
     2881 CAGATCCCAA TTTTGTTCGT ACTTTCCTTA CTACGTATCG TTCATTTTGT AAGCCACAGG 
     2941 AATTGCTAAG CTTGCTGATA GAACGGTTTG AAATTCCAGA TCCAGAGCCT ACTGAGGCCG 
     3001 ACAAACTGGC GCTAGAGAAA GGCGAGCAGC CAATCAGCGC AGATCTGAAA AGATTCCGCA 
     3061 AGGAATATGT CCAACCCGTG CAACTTAGGG TCTTGAATGT CTTTCGCCAC TGGGTTGAGC 
     3121 ATCATTTTTA TGACTTTGAA AGAGACTTGG AATTGCTTGA AAGACTGGAA GCCTTCATTT 
     3181 CAAGTGTAAG AGGGAAAGCC ATGAAGAAGT GGGTAGAATC CATTGCTAAA ATCATCAAGA 
     3241 GGAAGAAGCA AGCTCAGGCC AATGGAGTAA GCCATAATAT CACCTTTGAA AGTCCGCCTC 
     3301 CACCGGTTGA ATGGCACATA AGTAGGGCGG GACAGTCTGA AACCTTTGAC CTTATGACAC 
     3361 TTCATCCCAT AGAGATTGCA CGGCAGTTAA CACTCTTGGA ATCTGATCTC TACAGGAGAG 
     3421 TCCAGCCTTC TGAACTTGTA GGGAGTGTCT GGACCAAAGA AGATAAAGAA ATAAATTCTC 
     3481 CAAATCTATT AAAAATGATT CGCCATACAA CAAACCTCAC TTTGTGGTTT GAAAAATGCA 
     3541 TTGTGGAAGC AGAAAACTTT GAAGAACGGG TGGCAGTGCT CAGCAGAATA GTAGAAATTC 
     3601 TGCAAGTGTT TCAAGATCTG AATAATTTCA ATGGCGTGTT GGAGATAGTC AGTGCAGTCA 
     3661 ATTCAGTGTC AGTGTACAGA CTAGACCACA CATTTGAGGC ATTGCAGGAA AGGAAGCGGA 
     3721 GAGTTTTGGA TGACGCTGTG GAATTAAGTC AGGATCACTT TAAAAAATAC CTAGTAAAAC 
     3781 TTAAATCAAT CAATCCACCT TGTGTGCCTT TTTTTGGAAT ATATTTAACA AATATTCTGA 
     3841 AGACTGAAGA AGGGAACAGC GACTTTCTAA AGAGGAAAGG GAAAGATTTG ATCAATTTCA 
     3901 GTAAGAGGAG AAAAGTGGCT GAAATAACCG GAGAGATTCA GCAGTATCAG AACCAACCAT 
     3961 ACTGCTTACG GACAGAACCA GAGATGAGGA GATTCTTTGA AAACCTCAAT CCCATGGGAC 
     4021 TTTTATCTGA AAAAGAGTTT ACAGATTATT TGTTCAACAA ATCTCATGAA ATTGAACCCC 
     4081 GAAACTGCAA ACAACCACCT CGATTTCCTA GGAAGTCAAC CTTTTCCTTA AAATCTCCTG 
     4141 GAATAAGGCC CAATGCCAGC CGCCATGGCT CTACCTCAGG CACGTTCCGA GGTCACCCAA 
     4201 CCCCTCTGGA AAGGGAGCCT TATAAGATAA GCTTTAGCAG GATCGCCGAG ACGGAGCTAG 
     4261 AATCCACGGT GTCAGCACCG ACATCTCCCA ACACCCCATC CACCCCACCT GTGTCTGCTT 
     4321 CTTCAGACCA CAGCGTGTTT CTAGATGTGG ACCTCAATAG CTCCTGTGGC AGCAATACCA 
     4381 TCTTTGCTCC AGTCCTCTTG CCACACTCAA AGTCTTTCTT CAGCTCATGT GGAAGTCTAC 
     4441 ACAAACTGAG TGAAGAGCCA CTAGTTCCTC CTCCGCTTCC CCCTCGGAAA AAGTTTGATC 
     4501 ACGATGCTCC GAACTCCAAG GGAGCTATGA AATCGGATGA TGACCCTCCT GCTATTCCAC 
     4561 CAAGACAACC CCCTCCTCCA AAGGTAAAGC CAAGAGCTCC TGTCCTCACG GGTACATTTG 
     4621 AAGGGCCTGT GCCCAGTCCA CCTCCACCTC CTCCCAGAGA CCCTCTTCCT GATACTCCTC 
     4681 CACCAGTTCC TCTTCGGCCT CCGGAACACT TTATAAACTG TCCGTTTAAT CTCCAGCCGC 
     4741 CTCCGCTGGG ACATCCTCAC AGAGACCCAG ACTGGCTCAG AGACGTTAGC ACATGCCCTA 
     4801 ATTCACCAAG CACTCCTCCC CCTACGCCCT CGCCACGGAT TCCACGCAGA TGCCATTTGC 
     4861 CCAGCCCCAG TCACAACAAT CTTGCTCACC CTCCAGCTCC TCCCGTTCCA CCAAGGCAGA 
     4921 ATTCAAGCCC TCACCTACCA AAACTGCCAC CAAAGACTTA CAAGCGGGAG CTTTCACACC 
     4981 CGCCATTGTA CAGACTACCT TTGTTTGAAA ATGCAGAAAC TCCTCAGACG CGTACGCGGC 
     5041 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5101 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5161 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5221 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5281 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5341 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5401 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5461 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5521 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5581 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5641 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5701 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5761 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5821 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5881 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5941 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6001 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6061 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6121 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6181 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6241 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6301 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6361 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6421 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6481 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6541 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6601 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6661 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6721 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6781 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6841 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6901 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6961 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7021 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7081 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7141 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7201 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7261 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7321 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7381 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7441 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7501 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7561 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7621 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7681 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7741 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7801 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7861 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7921 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7981 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8041 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8101 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8161 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8221 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8281 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8341 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8401 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8461 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8521 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8581 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8641 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8701 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8761 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8821 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//