LOCUS       RR208272                8451 bp    DNA     circular  SYN 
DEFINITION  Fanca (Myc-DDK-tagged ORF) - Rat Fanconi anemia, complementation group A (Fanca), (10 ug)
ACCESSION   NM_001108455
VERSION     NM_001108455.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4608..4699 
                     /label="Myc-DDK"  
     misc_feature    4729..5314 
                     /label="PolyA signal"  
     misc_feature    4786..4805 
                     /label="Restriction Enzyme"  
     misc_feature    5590..6262 
                     /label="ColE1"  
     misc_feature    6692..7486 
                     /label="Kan/Neo"  
     misc_feature    7521..7823 
                     /label="SV40 ori"  
     misc_feature    7909..8386 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4590..4596 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4590 
                     /label="Fanca"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCAGGTC GCTGCTGATG TTCTGCAGAG AATGCTGGCT TTTTCACTCG 
     1081 ATACCCTGGA TGCTGATTCG CAGACGACTT TGGATCACCA GGCCGTGAGT GGCTGGATCA 
     1141 CTGTGTACTC TGGACGTACG TGCTGTGGTA TGATAGCAGC TGATTCTCTG AAGAGCTTCC 
     1201 TCAGTCACGC GCTAACTCAG ATTCTGACTC ACAAGCCCGT GCTGAAAGTG TCTGACGCCA 
     1261 TTCAAATGCA GAAAGAGTGG AGCTTTGCAA AGACACCCCA TCTGCTCATT GATCTGTACT 
     1321 GCAGGGTTTT TGTGACACTG GGTCCAGAGG AGTCAATTGG CCATTTGCAG GAAGTTCTGG 
     1381 AGCTGCAGGA GGTTAACTGG CAACACGTGC TGTCCTGTGT GTCTACACTG GTTGTCTGCT 
     1441 TTTCTGAGGC ACAGCAGCTG ATTAAAGATT GGGTGGCCTC TTTGATGGCC CGGGCCTTTG 
     1501 AGAGCTACCA CCTAGACAGC ATGGTCACTG CCTTCCTGAT TGTGCGCCAG GCTACTCTGG 
     1561 AAGGCCCCTA CGTATTCCCG TCCTACGCAG ATTGGTTCAA GGAGTCCTTT GGAAGCTCAC 
     1621 ATGGCTACCA TAGCTGCAGC AAGAAGACAC TGGTCTTCCT CTTCAAATTC CTGTCTGACC 
     1681 TCGTGCCCTG GGAAGCCCCT CGGCACATGC AGGTGCACAT TTTCCACCCA CCCCTGGTGC 
     1741 CAAGCAAGTA CCACTCTCTG CTCACAGACT ACATCTCCTT GGCCAAGACA AGGCTTGCTG 
     1801 ACCTCAAGGT TTCCCTGGAG AATGTAGGAC TCTACGAGGA TTTGTCATCC ACCGGGGACA 
     1861 TTGCTGAGCG TGAGAGCCAG GCAGTCCAGG ATGTTAAGAA GGCCATCATG GTGTTTGAAC 
     1921 AAACAGGGAA GATCCCTATG CCTGTCCTGG AGGCCAGTAT CTTCAGGAGG CCCTACTACC 
     1981 TGTCGCACTT CCTCCCTGCG CTGCTCGCAC CGAGAGTGCT CCCGGAAGTT CCTGACTCTC 
     2041 GGGTGGCCTT CATAGAGACC CTGAAGAGAG CAGATAAGAT TCCCCCGTCG TCATACAGTG 
     2101 CCTACCGCCA AGCCTGTGCC ACTGCTGGGG ACAAGCAGCC CGACAACACC ACCTCGGGAC 
     2161 AAAGGACAGA AACCAACTGT GCTGAACGGC CCCTGGGACT GCTCACGGCA GCACTGGAGG 
     2221 AGCTCAGAGC TCTAATGACA GACCCCACCC AATACAGTGT TATATCTGCT CAGGTGGCTG 
     2281 TGGTTTCTGA AAGACTAAAT GCTGCTCTGG GACACAGGAA CGATGATGAT GTCAGCCTCC 
     2341 GGAGATCAAA GATTCAGCTC AGTGTCCTTC TGCCCACACT CCAGAGTCAG GACCAGGGGG 
     2401 TTGTGGATCT CCTGCTCACA GCCTTCTGTC AGAACCTAAT GGCAGCCTGT AGCTTTGTGC 
     2461 CCCCAGAGAG GCAGAGCTCC TGGGCTGTCC TTTTCGTGAG GACCCTTTGT GGACACATGC 
     2521 TCCTCCCGGC AGTGCTCACT CGGCTGTGCC AGCTGCTCCG CCACCAGGGT CCAAACTTGA 
     2581 GTGCCCCTCA CGTACTGGGC TTGGCTGCTC TTGCTGTCCA TCTGGGCGAG TGCAGGTCCA 
     2641 CACTTCCAGA GGTGGATCCA GGTGTTCTCG CTCCTCCCAG CAGCCTCTGC GTCCCTGACT 
     2701 TCTTAAACAG CCTCCTGACC TGCCACACGA GGGACTCTCT GCTCTTCTGC ATGAAATTCT 
     2761 GCACAGCAGC AATTTCTTAC TGTTTATGCA AGTTCTCTGC TCAGCCCTCT GCTTCCCTGC 
     2821 ACAATTGTTT GTCTCCAGGT CTGATCCAAA AGTTTCAGTT CATTGTGTTA AGATTGTTCC 
     2881 CAGAGGCCAG AGCACCTTGC AGTCCAGAGC ACACAGCTCG CATTCCCTGG AAACTTCTGT 
     2941 ATCTTCCTTC TGCAGACTGG CCGAGAGCTG CGCTCTCTCT GTGGAGATGG AGCAGCTTCC 
     3001 AAGAGCTGTT AAAGGAGGAG GAATTTCATT TAACTTACAG AGATTGGATT CAGTTGGAAC 
     3061 TGGAAATTCA ACCTGAAGCT GATGCCCTCT CAGGCACAGA AAGACATGAC TTCCACCAGT 
     3121 GGGCAATCTA TGAGCGCTAC CTGCCAGCAC CGTCAGCTAT GGGAGGCTGT GGTGGGGACC 
     3181 TGGAGGCGGC GTGCACAGTC CTTGTCAGCG AGCTCATGGA CTTCCACCAA AGGAGTTACA 
     3241 ACCACTCAGA GGACTCTGAT CTGGTTCTTG GTGGCCGCAC AGGAAACGAG GATCTTCTTT 
     3301 CTAGACTACA GGAGATGGCT ATGGACCTGG AGCTTGATCA AGGTTCTGCT GTGCCCCGTG 
     3361 GCTGTTCTAC TCCTGAGAGT CACTTTCTCT TTCGGGTGTT CAGAGGACGG CTGCAAGCTT 
     3421 TGGCAAGGCC AGGCAGCATG GCTACCAGCC TTAGGAGACA GCAGGAACTG CTCATATGCA 
     3481 AACGGCTTCT CCTCCGTCTG CCTCCCTCTG TCCTTGTGGG CAGCCCCCAG GCCGGACAAC 
     3541 CTGTCTCCCC GAACTGTGAA GAGTTCTTTA ACCTGGTCAC CTCTGGGTTG AGAAACATCT 
     3601 GTTGCCACGG CAGTGTCCTG ACCTACGACC TTACCATCCA CTTCTTCAGG GGGCTCCTCA 
     3661 ATGTGTGTCT CCGAAGTCAA GACCCGGCGC TGGTGGCCAA CCGCACACTG GCAGAATGCC 
     3721 AGACTAAGTG TCCCATGATT CTGACTTCAG CACTGCTGTG GTGGTCAAGC CTGGAACCGG 
     3781 TGTTGTGTAG TCAGTGGAAG AAATGCTATC AGAGCACACT GCCTCAAGAG CTGCAGAGGC 
     3841 TACAGGAAGC CCGGAAGTTT GCTAGCAATT TCGCCTCCGA TTCAGCGGCC CCAGCACCCA 
     3901 GCCCCGCCTG GATCTCAGCT GCTGCACTGT ACTTTGCAAC TAGAGAAGCT GGGAGGGAAA 
     3961 ATGTCACAAC ACATCTGAAG AGGCTAGACT GCGAAAGAGA GGAGCTCCTG ATCACCCTCC 
     4021 TCTTCTTTTC CTTGATGGGG CTACTCTCGT CCTACCTGAC CCAACGGGAC ACCGCTGAGT 
     4081 ACCAGAAGGC TGTGGACATC TGTGCTGAGG TCCTCGCCTG TTTGGAAAGA CGGAAGGTGT 
     4141 CCTGGCTGAT GCTCTTCCAG TTGGCAGAGA CGGATGCCAA GCTAGGGCAC CTCCTTCTCT 
     4201 TGGCCCCAGA CCAGCACACC AGGCTGCTAC CACTTGCTTT CTACAGTCTC CTCTCCTGCT 
     4261 TCAGTGACGG TACCACTGTC AGGGAAGCAG CTTTCCTGCA TGTTGCGGTT GACATGTACC 
     4321 TCAAACTGGT ACAGCTCTTT GTGGATGGGG AAACCAGGAT TGTGTTACCT CAGGCCAGCA 
     4381 GAAGCATCTC CGAGCTCCAG GATAGCCCTG TACAACTGAT AACAAAGGCA CGCAGTTTTC 
     4441 TGTTGCAGCT AATACCTCAG TGTCCGAGAC AGTGCTTCTC CAACATGACA GAGCTGCTGG 
     4501 CTGGTCGAGG AGACTGTGAC CCTGAGGTGA GCAACGCCCT CCAGCAAAGG CAGCAGGCTG 
     4561 GCCCCAGCTT TGACCTCTCC CAGGAGCCTC ATCTGTTCAC GCGTACGCGG CCGCTCGAGC 
     4621 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4681 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4741 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4801 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4861 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4921 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4981 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5041 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5101 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5161 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5221 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5281 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5341 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5401 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5461 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5521 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5581 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5641 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5701 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5761 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5821 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5881 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5941 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6001 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6061 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6121 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6181 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6241 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6301 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6361 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6421 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6481 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6541 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6601 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6661 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6721 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6781 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6841 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6901 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6961 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7021 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7081 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7141 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7201 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7261 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7321 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7381 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7441 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7501 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7561 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7621 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7681 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7741 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7861 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7921 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7981 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8041 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8101 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8161 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8221 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8281 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8341 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8401 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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