LOCUS       RR208260                8151 bp    DNA     circular  SYN 
DEFINITION  Adcy2 (Myc-DDK-tagged ORF) - Rat adenylate cyclase 2 (brain) (Adcy2), (10 ug)
ACCESSION   NM_031007
VERSION     NM_031007.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4308..4399 
                     /label="Myc-DDK"  
     misc_feature    4429..5014 
                     /label="PolyA signal"  
     misc_feature    4486..4505 
                     /label="Restriction Enzyme"  
     misc_feature    5290..5962 
                     /label="ColE1"  
     misc_feature    6392..7186 
                     /label="Kan/Neo"  
     misc_feature    7221..7523 
                     /label="SV40 ori"  
     misc_feature    7609..8086 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4290..4296 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4290 
                     /label="Adcy2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGCGGCGC CGCTACCTGC GGGACCGCGC CGAGGCGGCG GCGGCAGCGG 
     1081 CGGCGGGAGG CGGAGAGGGG CTGCAGCGGT CCCGGGACTG GCTCTACGAG TCCTACTACT 
     1141 GCATGAGCCA GCAGCACCCG CTCATCGTCT TCCTGCTGCT CATCGTCATG GGCGCCTGCC 
     1201 TCGCCCTGCT AGCCGTCTTC TTCGCGCTCG GGCTGGAGGT GGAAGACCAT GTGGCATTTT 
     1261 TAATAACGGT TCCCACTGCC CTGGCCATTT TCTTTGCCAT ATTCATTCTT GTCTGCATAG 
     1321 AGTCTGTGTT CAAGAAGCTA CTCCGTGTGT TTTCGCTGGT GATTTGGATA TGTCTGGTTG 
     1381 CCATGGGATA CCTGTTCATG TGCTTCGGAG GGACTGTGTC TGCCTGGGAC CAGGTGTCAT 
     1441 TCTTCCTCTT CATCATCTTT GTGGTATATA CCATGCTTCC CTTCAACATG CGAGATGCCA 
     1501 TCATTGCCAG CATCCTCACA TCTTCATCTC ATACGATAGT GCTGAGCGTC TACCTGTCTG 
     1561 CAACACCAGG GGCCAAGGAG CACCTGTTCT GGCAGATACT GGCCAATGTG ATCATTTTCA 
     1621 TTTGTGGGAA CTTGGCGGGA GCCTACCACA AGCACCTCAT GGAGCTTGCC TTGCAGCAAA 
     1681 CCTATCGGGA CACGTGTAAT TGCATCAAGT CCCGGATCAA GCTGGAATTT GAAAAACGGC 
     1741 AGCAGGAACG GCTCCTGCTC TCCTTGCTGC CAGCTCACAT CGCCATGGAG ATGAAAGCTG 
     1801 AAATCATTCA GAGGCTGCAG GGCCCCAAAG CAGGACAGAT GGAAAACACA AACAACTTCC 
     1861 ACAATCTGTA TGTCAAACGA CACACCAACG TGAGCATATT ATACGCTGAC ATTGTTGGCT 
     1921 TCACCCGCCT TGCAAGCGAT TGCTCCCCTG GCGAACTGGT CCACATGCTG AATGAACTCT 
     1981 TTGGGAAGTT TGATCAAATA GCAAAGGAGA ATGAATGCAT GAGAATTAAA ATTTTAGGAG 
     2041 ACTGCTATTA CTGTGTTTCC GGGCTCCCTA TATCACTCCC TAACCATGCC AAGAACTGTG 
     2101 TGAAAATGGG ATTGGATATG TGCGAAGCCA TAAAGAAAGT GAGGGATGCT ACCGGAGTTG 
     2161 ATATCAACAT GCGTGTAGGA GTGCATTCTG GGAACGTTCT CTGTGGTGTG ATTGGTCTCC 
     2221 AGAAGTGGCA GTATGATGTG TGGTCTCATG ATGTTACTCT GGCAAACCAC ATGGAAGCTG 
     2281 GAGGAGTCCC TGGGCGTGTT CACATTTCTT CAGTCACTCT GGAGCACTTG AATGGGGCTT 
     2341 ATAAAGTGGA GGAAGGAGAT GGTGAGATAA GAGACCCATA TTTAAAGCAG CACTTGGTGA 
     2401 AAACCTACTT TGTAATCAAT CCCAAGGGAG AGCGACGGAG TCCTCAGCAT CTCTTCAGAC 
     2461 CTCGACACAC TCTGGACGGA GCCAAGATGA GAGCATCTGT CCGCATGACC CGGTACTTGG 
     2521 AGTCCTGGGG AGCAGCCAAG CCATTCGCAC ATCTGCACCA CAGAGATAGC ATGACCACAG 
     2581 AGAATGGGAA GATTAGTACC ACGGATGTGC CAATGGGTCA ACATAATTTT CAAAATCGCA 
     2641 CCTTAAGAAC TAAGTCACAG AAGAAGAGAT TTGAAGAAGA ACTGAATGAA AGGATGATCC 
     2701 AAGCAATTGA TGGGATCAAT GCACAGAAGC AATGGCTCAA GTCAGAAGAC ATTCAAAGAA 
     2761 TCTCCCTGCT TTTCTATAAC AAGAATATAG AGAAAGAATA CCGAGCTACT GCACTGCCAG 
     2821 CATTCAAGTA CTACGTGACC TGTGCCTGCC TCATCTTTCT CTGCATCTTC ATTGTACAGA 
     2881 TACTTGTATT GCCAAAAACG TCCATCCTTG GCTTCTCCTT TGGAGCTGCA TTTCTCTCCC 
     2941 TCATCTTCAT CCTCTTTGTC TGCTTCGCTG GACAGCTTTT GCAATGCAGC AAAAAGGCCT 
     3001 CCACCTCTCT CATGTGGCTT TTGAAATCAT CAGGCATCAT CGCCAACCGC CCATGGCCAC 
     3061 GGATCTCCCT CACAATCGTC ACCACGGCTA TCATACTAAC CATGGCTGTG TTCAACATGT 
     3121 TTTTCCTGAG CAACTCTGAG GAGACAACCC TTCCCACTGC CAATACATCA AATGCAAACG 
     3181 TTTCTGTCCC GGATAACCAG GCGTCGATTC TTCATGCTCG AAACTTGTTT TTCCTCCCGT 
     3241 ACTTCATATA CAGCTGCATC CTGGGCTTGA TCTCCTGCTC CGTTTTCCTG AGGGTGAACT 
     3301 ATGAGTTAAA AATGTTAATC ATGATGGTGG CACTCGTGGG CTACAACACC ATTCTACTCC 
     3361 ACACCCATGC CCATGTTCTG GATGCGTACA GCCAGGTCCT GTTTCAGAGA CCAGGCATTT 
     3421 GGAAAGACCT GAAGACCATG GGCTCCGTGT CACTCTCCAT ATTCTTCATC ACGCTGCTGG 
     3481 TTCTGGGCAG ACAGAGTGAA TATTACTGTA GGTTAGACTT CTTGTGGAAG AACAAGTTCA 
     3541 AAAAAGAGCG GGAGGAGATA GAAACCATGG AGAACCTAAA TCGAGTGCTG CTGGAGAACG 
     3601 TGCTTCCTGC ACACGTGGCT GAACACTTCC TGGCCAGGAG CCTGAAAAAT GAGGAGCTGT 
     3661 ACCACCAGTC CTACGACTGT GTCTGTGTCA TGTTTGCCTC CATTCCGGAC TTCAAGGAGT 
     3721 TCTACACAGA GTCAGATGTG AACAAGGAAG GCTTGGAATG CCTGCGGCTC CTGAATGAGA 
     3781 TCATTGCTGA CTTTGATGAT CTGCTTTCTA AGCCAAAGTT CAGTGGTGTT GAAAAGATCA 
     3841 AGACCATTGG GAGCACATAC ATGGCAGCCA CGGGACTGAG TGCCATACCC AGCCAGGAGC 
     3901 ACGCCCAGGA ACCTGAGCGT CAGTACATGC ACATAGGCAC CATGGTGGAG TTTGCATATG 
     3961 CCCTGGTGGG AAAACTGGAT GCCATCAATA AGCACTCCTT CAACGACTTC AAACTGCGAG 
     4021 TGGGTATCAA CCATGGGCCT GTAATAGCTG GCGTCATAGG GGCTCAAAAG CCACAGTATG 
     4081 ACATCTGGGG CAACACTGTC AACGTGGCCA GCAGAATGGA CAGCACCGGG GTCCTGGACA 
     4141 AAATACAGGT GACTGAGGAG ACAAGCCTCA TCTTGCAGAC GCTTGGCTAC ACGTGTACAT 
     4201 GTCGAGGTAT CATCAATGTG AAGGGGAAAG GGGACCTGAA GACATATTTT GTAAACACAG 
     4261 AGATGTCAAG GTCCCTTTCT CAGAGCAACT TGGCATCCAC GCGTACGCGG CCGCTCGAGC 
     4321 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4381 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4441 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4501 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4561 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4621 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4681 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4741 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4801 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4861 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4921 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4981 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5041 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5101 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5161 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5221 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5281 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5341 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5401 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5461 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5521 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5581 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5641 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5701 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5761 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5821 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5881 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5941 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6001 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6061 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6121 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6181 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6241 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6301 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6361 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6421 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6481 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6541 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6601 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6661 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6721 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6781 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6841 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6901 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6961 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7021 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7081 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7141 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7201 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7261 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7321 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7381 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7441 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7501 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7561 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7621 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7681 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7741 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7801 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7861 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7921 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7981 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8041 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8101 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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