LOCUS       RR208196                7905 bp    DNA     circular  SYN 
DEFINITION  Usp36 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 36 (Usp36), (10 ug)
ACCESSION   NM_001107069
VERSION     NM_001107069.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4060..4151 
                     /label="Myc-DDK"  
     misc_feature    4181..4766 
                     /label="PolyA signal"  
     misc_feature    4238..4257 
                     /label="Restriction Enzyme"  
     misc_feature    5042..5714 
                     /label="ColE1"  
     misc_feature    6144..6938 
                     /label="Kan/Neo"  
     misc_feature    6973..7275 
                     /label="SV40 ori"  
     misc_feature    7361..7838 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4035..4042 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4035 
                     /label="Usp36"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCATCGTG GATAAGCTGA AGGAGGCTCT AAAGCCTGGC CGCAAGGACT 
     1081 CGGCAGAGGA TGGGGACCTG GGCAAGCTGC TGGCGGCCTC CGCCAAGAAG GTCCTCCTGC 
     1141 AGAGGATTGA GTTTGAACCG GCCAGCAAGA GCTTCTCTTA CCAGCTGGAG TCCTTGAAGA 
     1201 GCAAATATGT ACTGTTGAAC CCCAAGGCTG AGGGTGCCGG GCGCCACAGG AGCGGAGATG 
     1261 AGCCACAGGC CAGGAAACCA GGCACCGAGC GCATGTCTGG AAGTGGAGGC GATGGAGTCC 
     1321 CTGCCCCACA GAAAGTGCTC TTCCCTGTGG AGCGACTGTC CTTGAGGTGG GAACGCGTAT 
     1381 TCCGAGTTGG TGCTGGGCTG CACAACCTGG GTAACACCTG CTTCCTGAAC TCCACCATTC 
     1441 AGTGCTTGAC TTACACGCCA CCTCTGGCCA ACTACTTGCT CTCCAAGGAG CACGCACGGA 
     1501 GCTGTCACCA AGGAAGCTTC TGCATGCTGT GCGTCATGCA GAACCACATG GTCCAGGCCT 
     1561 TTGCCAACAG TGGCAATGCC ATCAAGCCTG TCTCCTTCAT CAGAGACCTG AAAAAGATTG 
     1621 CCCGGCACTT CCGATTTGGG AACCAAGAGG ATGCACATGA ATTCCTGCGG TACACCATCG 
     1681 ACGCCATGCA GAAGGCCTGC TTGAATGGCT ATGCTAAGTT GGATCGGCAG ACACAGGCTA 
     1741 CTACCCTGGT GCATCAGATC TTTGGAGGCT ATCTCAGGTC CCGAGTGAAG TGTTCCGTGT 
     1801 GCAGAAGCGT CTCAGACACA TACGACCCCT ACTTGGACAT AGCGCTGGAG ATTCGGCAAG 
     1861 CTGCAAATAT TGTGCGTGCT CTGGAACTTT TTGTGAAATC AGATGTCCTG AGTGGAGAGA 
     1921 ACGCCTATAT GTGTGCTAAG TGTAAGAAGA AGGTTCCAGC TAGCAAGCGC TTCAGCATCC 
     1981 ATAGAACATC CAATGTCCTG ACTCTGTCCC TCAAGCGCTT TGCCAACTTC AGTGGGGGGA 
     2041 AGATCACCAA GGATGTTGGC TATCCAGAGT TCCTGAACAT CCGTCCATAC ATGTCGCAGA 
     2101 GCAGTGGAGA CCCTGTCATG TATGGGCTCT ATGCTGTTCT GGTGCACTCA GGCTACAGCT 
     2161 GCCACGCGGG GCACTACTAC TGCTACGTGA AGGCCAGCAA TGGACAGTGG TACCAGATGA 
     2221 ACGACTCCTT GGTCCATTCC AGCAATGTCA AGGTGGTTCT GAGCCAGCAG GCCTACGTGC 
     2281 TCTTCTATCT GCGGATTCCA GGCTCTAAGA AAAGTCCTGA GGGCCCTGTC TCCAGGGTGG 
     2341 GTGCCACTCT TCCTAGCCGT CCCAAGGTCA TTCCAGAGCA CCCCAAGAAG AGCCCTGGCA 
     2401 ATGGGGTTGT TTCCTCCCCA CTGATGGCAA AGCGACAAGA CTCTCTAATG ATGAGAAAGT 
     2461 TGCAGTCCCC TGAGGAGGTT GGCATGCCTG TGTCCAGAAA TGGCTCCATT TCTGGTCTGA 
     2521 AGTTACAGAA TGGATGTGCT CCTGCAAAGT CACCTGCCGG GTCCCCCTCC CCGAGACTCG 
     2581 CTCCGACACC CACACATATG CCCACCATTC TGGACGAGCC TGGGGAGAAG GTGAAGAAGT 
     2641 CAGCCCCGCC ACAGTCTCGC ACCACGTCCC CAACTGCTTC TCAGGGCTTT CCTGGGACCG 
     2701 GCGAGTCCAG GAGCCAGCGG CCTGGCTCCT GGGCGAGCAG AGACAGCATC TTCTCTACCT 
     2761 CACCAAAGCT GGCCAAAGCC ATTACTAACG GGCACAGGCT GAAGGGGGAA GGCAATGGTG 
     2821 TGGACCTGGA GAAGGGGGAT TCCAGCAGCT CGAGCCCCGA GCACTCTGCC AGCAGTGACG 
     2881 CTGCCAAGGC CCCGCAGACC TCAGAGGGCA GAGCTGCACA TGTCTGTGAT TCTCAGGGAA 
     2941 CAAACTGCCC AGCCACTGGC CATTCAAAAG TGCTGTCGAA CGGAGTCGAT GCCAAGATGG 
     3001 GGAAGTTGAA GTCCCCTGCC CTGAGCAGCA CCACCACCGA GCCCACAAGC CTCATGTCGC 
     3061 CTCCACCAGC CAAAAAACTG GCCCTGTCAG CCAAGAAGGC CAGCACCCTG CGGAGGGCGA 
     3121 CCGGCAATGA CATCGGTTCA CCTTCCCCCT CAGCATTCTG CGATCTCACC TCCCCCATGA 
     3181 AAGCCACCCA CCCCGTCGTT GCCTCCCCTG GGCCTGTCAG TAAAACCAGG GCTGCTGCCG 
     3241 CACCTGCTCC CCAACCGTCT CCCCACCCCC ACTCTGCATC ACTGTCCAGT AGTAGCGCCA 
     3301 AGCCCTTAGG GACATCAGAG CCACAGAGCT GCTGCTCCTC TGCCTGGACA CCCCTGCCTC 
     3361 AGGTCAATGG ACACTTCACG TCCCACTTAC ACCAACTGCC AGAGGCCAAT GAGGCCCTAC 
     3421 ACAGCCCCTC CAGAAAGAGG AAGAAGACTC CTAGTGGAGA TCCCCAGAGA CTGGGCAGAG 
     3481 ACACACACCT CCCACAGTGC CTCGAGGGAG CACCTGCAAC AGCACGCAGG AAGAAGAGGA 
     3541 AGAAGAAGAA GGACCTGGAG GGTGTGGGCG ACACAGCTAC CCAGCAGGAA AGCCAGTCTC 
     3601 AGGACCAGCC TTGGAGTCCC AGGGGCCGGA AGGAAGAGGG TACGCAGCTT CAGGTAAATG 
     3661 GCCATCAAGT GAGTCACATC CTGGACAGTT ACCATGCGAG CAGCAGGAAA AGGAGGAAGA 
     3721 GAAAAAGATC AGAAGGGCTC AGCCAAGAAG CCACCGTGTG CCAGGACCTA CTTCAGCATG 
     3781 GCTGCTCCCC TGCAGACCAC TGGCTGGGAC GGGCACCTCT TGTTACCTGG AACAGAGATC 
     3841 AAGAACCGGA TGTGGTCCAG GAGTTGCTTC AGGACTCGTC TGATACGGCT TATGGGAAAA 
     3901 AAGTTTTGAC CTGGGATGGT GAGCCTTCAG CCATCAGTCA GGACGCCATG AAAGACAGCA 
     3961 GACTGGCCCG GACCCAGACC GTGGTTGATG ACTGGGATGA AGAGTTTGAT CGAGGGAAGA 
     4021 AAAAAGGAGA GAACAGTGCT CCAAGCGGAC CGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4081 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4141 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4201 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4261 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4321 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4381 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4441 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4501 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4561 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4621 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4681 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4741 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4801 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4861 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4921 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4981 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5041 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5101 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5161 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5221 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5281 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5341 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5401 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5461 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5521 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5581 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5641 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5701 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5761 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5821 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5881 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5941 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6001 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6061 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6121 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6181 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6241 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6301 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6361 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6421 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6481 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6541 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6601 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6661 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6721 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6781 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6841 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6901 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6961 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7021 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7081 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7141 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7201 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7261 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7321 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7381 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7441 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7501 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7561 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7621 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7681 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7741 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7801 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7861 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//