LOCUS       RR208191                6653 bp    DNA     circular  SYN 
DEFINITION  Sacm1l (Myc-DDK-tagged ORF) - Rat SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), (10 ug)
ACCESSION   NM_053798
VERSION     NM_053798.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2810..2901 
                     /label="Myc-DDK"  
     misc_feature    2931..3516 
                     /label="PolyA signal"  
     misc_feature    2988..3007 
                     /label="Restriction Enzyme"  
     misc_feature    3792..4464 
                     /label="ColE1"  
     misc_feature    4894..5688 
                     /label="Kan/Neo"  
     misc_feature    5723..6025 
                     /label="SV40 ori"  
     misc_feature    6111..6588 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2792..2798 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2792 
                     /label="Sacm1l"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCACA GCCTACGAGC ATCTGAAGCT GCATATTACA CCTGAAAAGT 
     1081 TTTATGTGGA AGCTTGTGAT GATGGAGCAG ATGATGTACT CATCATTGAC CGCGTGTCTA 
     1141 CAGAGGTCAC CCTTGCAGTC AAAAAAGATG TTCCCCCTTC TGCTGTTACA AGACCAATAT 
     1201 ATGGGATAAT GGGCACAATC CATCTGGTGG CAGGCAATTA TCTTGTTGTC ATTACCAAAA 
     1261 AGATGAAAGT AGGTGAATTT TTTAATCATG TAATCTGGAA AGCCACAGAT TTTGATGTTC 
     1321 TTTCTTATAA GAAGACAATG TTACACTTAA CTGATATTCA GTTACAAGAT AATAAAACTT 
     1381 TCCTAGCAAT GCTAAACCAT GTCTTGAGTA CGGATGGATT TTACTTTTCA ACAACATATG 
     1441 ACTTGACCCA TACTTTGCAA CGGCTATCTA ACACGAGTCC AGAATTCCAA GAAATGAGTC 
     1501 TCCTAGAAAG GGCAGATCAG CGGTTTGTAT GGAACGGGCA TCTGCTCAGA GAACTGTCTG 
     1561 CTCAGCCAGA GGTCCATCGG TTTGCTCTTC CTGTGTTGCA TGGCTTTATT ACTATGCATT 
     1621 CATGTTCTAT TAACGGGAAA TATTTTGATT GGATTCTCAT CTCCCGGAGG AGTTGTTTCA 
     1681 GAGCCGGTGT GCGTTATTAT GTGAGAGGAA TTGATTCTGA AGGCCACGCA GCTAACTTTG 
     1741 TAGAAACAGA ACAGATTGTG CACTACAGTG GGAACAGGGC TTCATTTGTA CAGACTCGAG 
     1801 GATCAATACC TGTTTTCTGG TCACAAAGAC CAAATCTAAA GTACAAACCA GATCCACAGA 
     1861 TCAACAAAGT AGCAAATCAC ATGGATGGTT TCCAAAGGCA TTTTGATTCA CAAGTAATTA 
     1921 TTTATGGAAA ACAAGTAATA ATTAATCTGG TGAACCATAA GGGATCAGAG AAACCACTTG 
     1981 AGCAAACATT TGCAAAAATG GTGTCATCCT TGGGAAGTGG GATGATCAGA TATATTGCCT 
     2041 TTGACTTCCA TAAGGAGTGT AAAAATATGA GATGGGATCG ACTGAGTATT TTATTGGATC 
     2101 AAGTAGCAGA AATGCAGGAT GAATTAAGTT ATTTTTTAGT AGACTCTGCT GGCAAGGTGG 
     2161 TGACAAACCA GGAAGGAGTT TTCCGCAGCA ACTGTATGGA TTGTCTAGAC AGAACGAATG 
     2221 TGATACAGAG TTTATTAGCT CGGCGTTCCC TTCAGGCACA GCTGCAGAGA CTAGGAGTTT 
     2281 TGCATGTGGG ACAAAAGCTT GAAGAACAAG ATGAGTTTGA GAAGATTTAC AAAAATGCCT 
     2341 GGGCCGATAA TGCTAATGCT TGTGCCAAAC AGTATGCAGG AACCGGTGCC TTGAAGACTG 
     2401 ACTTTACAAG AACTGGAAAA AGAACTCAGT TGGGACTGGT AATGGATGGC TTCAACTCAT 
     2461 TATTACGGTA CTACAAGAAC AACTTTTCTG ATGGATTTAG ACAAGATTCT ATAGACTTAT 
     2521 TTCTTGGAAA CTACTCAGTG GATGAATTAG ACTCTCACAG TCCTTTAAGT GTTCCAAGGG 
     2581 ACTGGAAATT CCTGGCGTTG CCTATCATCA TGGTTGTTGC CTTTTCAATG TGCATCATCT 
     2641 GTTTGCTTAT GGCTGGTGAC ACTTGGACAG AAACACTGGC ATATGTCCTC TTCTGGGGAG 
     2701 TTGCAAGCAT TGGAACATTT TTTATTATTC TTTACAATGG CAAAGATTTT GTTGATGCTC 
     2761 CCAGACTTGT ACAGAAAGAA AAGATAGACA AAAGATAGAC ACGCGTACGC GGCCGCTCGA 
     2821 GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA 
     2881 CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT 
     2941 GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC 
     3001 CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT 
     3061 ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT 
     3121 GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA 
     3181 CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG 
     3241 GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT 
     3301 TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC 
     3361 CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT 
     3421 TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC 
     3481 AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC 
     3541 AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG 
     3601 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 
     3661 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 
     3721 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 
     3781 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 
     3841 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 
     3901 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 
     3961 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 
     4021 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 
     4081 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 
     4141 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 
     4201 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 
     4261 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 
     4321 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 
     4381 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 
     4441 ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT 
     4501 GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG 
     4561 CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT 
     4621 TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG 
     4681 CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG 
     4741 AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG 
     4801 ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG 
     4861 TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA 
     4921 GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA 
     4981 AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT 
     5041 CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT 
     5101 TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT 
     5161 CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT 
     5221 CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC 
     5281 GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA 
     5341 TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT 
     5401 GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA 
     5461 CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA 
     5521 GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG 
     5581 ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA 
     5641 ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC 
     5701 GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG 
     5761 CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA 
     5821 AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG 
     5881 ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA 
     5941 CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC 
     6001 ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC 
     6061 ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA 
     6121 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     6181 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     6241 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     6301 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     6361 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     6421 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     6481 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     6541 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     6601 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
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