LOCUS       RR208173                6867 bp    DNA     circular  SYN 
DEFINITION  Ggt7 (Myc-DDK-tagged ORF) - Rat gamma-glutamyltransferase 7 (Ggt7), (10 ug)
ACCESSION   NM_130423
VERSION     NM_130423.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3024..3115 
                     /label="Myc-DDK"  
     misc_feature    3145..3730 
                     /label="PolyA signal"  
     misc_feature    3202..3221 
                     /label="Restriction Enzyme"  
     misc_feature    4006..4678 
                     /label="ColE1"  
     misc_feature    5108..5902 
                     /label="Kan/Neo"  
     misc_feature    5937..6239 
                     /label="SV40 ori"  
     misc_feature    6325..6802 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3006..3012 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3006 
                     /label="Ggt7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCAGAG AACGAGGCTA GCCAGGAGAG CGCCCTAGGC GCCTACTCAC 
     1081 CAGTAGACTA CATGAGCATC ACCAGCTTCC CGAGGCTGCC CGAGGACGAG CCAGCGCCTG 
     1141 CGGCCCCGCT GAGGGGCCGT AAGGACGAGG ATGCCTTCCT GGGAGACCCG GATACTGATC 
     1201 CGGACTCCTT CCTGAAGTCT GCCCGGCTTC AGCGATTGCC TTCATCTTCC TCGGAGATGG 
     1261 GCAGCCAGGA CGGGTCACCA CTTCGCGAGA CGCGCAAGGA CCCATTCTCA GCTGCAGCAG 
     1321 CCGAGTGTTC CTGCCGCCAG GACGGGCTCA CGGTCATCGT CACCGCCTGC CTCACTTTTG 
     1381 CCACGGGTGT TACCGTGGCG CTGGTCATGC AAATCTACTT CGGGGATCCC CAGATCTTCC 
     1441 AGCAAGGTGC TGTGGTGACG GATGCCTCCT GTTGCACGGC GCTGGGCATG GAGGTGCTCA 
     1501 GTAAACAGGG CTCTTCCGTG GACGCAGCTG TGGCAGCAGC CTTGTGTTTG GGAATAGTGG 
     1561 CTCCACACAG TTCTGGCCTG GGCGGGGGAG GCGTGATGCT GGTACACGAT ATCCGCCGGA 
     1621 ATGAGAGCCA CCTAATTGAT TTCCGGGAGT CTGCACCTGG GGCCCTCAGA GAAGAGGCCC 
     1681 TGCAGAGATC CTGGGATACC AAGCCTGGGC TCTTGGTGGG GGTCCCCGGA ATGGTGAAAG 
     1741 GGCTATATGA AGCTCATCAA CTCTATGGCA GGCTGCCCTG GTCCCAAGTC CTGGCCTTCG 
     1801 CAGCAGCTGT GGCCCAAGAT GGCTTCAACG TGACCCATGA TCTAGCTCAT GCCTTGGCTG 
     1861 AGCAGCTGCC TCCCAATGCA TCTGACCGCT TCCTGGAAAC TTTCCTGCCG TTGGGCCACC 
     1921 CACCTTTGCC TGGCTCCCTG CTGAGACGGC CTGATCTCGC CGAGGTGCTG GATATACTTG 
     1981 GCATCTCTGG CCCTGCTGCC TTCTACAATG GTGGCAACCT CACGCTGGAG ATGGTGGCTG 
     2041 AGGTTCAGCA TGCAGGGGGT GTCATGACCG AGGAGGACTT CAGCAACTAT AGTGCTCTCA 
     2101 CGGAGAAGCC TGTGTGTGGA GTATACCGAG GCCACCTGGT TCTCAGTCCC CCACCCCCAC 
     2161 ACACAGGCCC GGCCCTCATC AGTGCGCTCA ACATCCTGGA GGGCTTCAAC CTCACCAGCC 
     2221 TGGTATCCCG CGAGCAAGCG CTTCACTGGG TAGCAGAGAC CCTGAAGATT GCATTGGCCC 
     2281 TGGCTAGCAG ACTGGGAGAC CCTGTCTATG ATTCTACCAT CAGTGAGAGC ATGGACGACA 
     2341 TGCTCAGCAA GGTGGAGGCT GCCAACTTCC GGGGCCACAT CAGTGACTCT CAGGCAGCCC 
     2401 CTGCCCCACT CCTGCCTGTC TATGAGCTGG ACGGGGCTCC CACAGCTGCC CAGGTGCTGG 
     2461 TCATGGGCCC TGACGACTTC ATCGTGGCCA TGGTCAGCTC CCTGAATCGG CCTTTTGGAA 
     2521 GTGGCCTCCT CACTCCCTCC GGGATCCTTC TCAACAGCCA GATGCTAGAC TTCTCCTGGC 
     2581 CCAATAGGAC TGCTAACCAC TCTGCACCCA GCCTGGAGAA CTCGGTACAG CCAGGGAAGC 
     2641 GGCCCCTCTC TTTCCTTCTG CCCACTGTAG TCCGACCAGC AGAAGGGCTC TGTGGGACCT 
     2701 ACCTCGCTCT GGGGGCCAAC GGAGCTGCTC GAGGCCTCAG TGGCCTGACC CAGGTTCTAC 
     2761 TGAATGTCCT GACCTTGAAT CGGAACCTGA GTGACAGCCT GGCCAGAGGA CGCCTCCACC 
     2821 CAGACCTGCA GTCCAACGTC CTGCAGGTGG ACAGTGAGTT CACCGAGGAA GAGATTGAGT 
     2881 TCCTGGAAGC CAGGGGTCAC CATGTGGAGA AGGTAGATGT CTTATCCTGG GTCCACGGCA 
     2941 GTCGGAGAAC CAACAACTTC ATCATTGGTG TGAAGGACCC TCGGAGCCTG GATGCAACCG 
     3001 GAGCCAGCAT CCTGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3061 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3121 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3181 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3241 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3301 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3361 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3421 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3481 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3541 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3601 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     3661 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     3721 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     3781 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     3841 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     3901 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     3961 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4021 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4081 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4141 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4201 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4261 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4321 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4381 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4441 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4501 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4561 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4621 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     4681 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     4741 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     4801 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     4861 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     4921 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     4981 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5041 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5101 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5161 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5221 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5281 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5341 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5401 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5461 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5521 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5581 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     5641 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     5701 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     5761 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     5821 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     5881 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     5941 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6001 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6061 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6121 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6181 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6241 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6301 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6361 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6421 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6481 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6541 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6601 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     6661 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     6721 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     6781 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     6841 ATTTAACAAA AATTTAACGC GAATTTT
//