LOCUS       RR208161                8169 bp    DNA     circular  SYN 
DEFINITION  Ppp1r3a (Myc-DDK-tagged ORF) - Rat protein phosphatase 1, regulatory (inhibitor) subunit 3A (Ppp1r3a), (10 ug)
ACCESSION   NM_001109222
VERSION     NM_001109222.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4326..4417 
                     /label="Myc-DDK"  
     misc_feature    4447..5032 
                     /label="PolyA signal"  
     misc_feature    4504..4523 
                     /label="Restriction Enzyme"  
     misc_feature    5308..5980 
                     /label="ColE1"  
     misc_feature    6410..7204 
                     /label="Kan/Neo"  
     misc_feature    7239..7541 
                     /label="SV40 ori"  
     misc_feature    7627..8104 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4308..4314 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4308 
                     /label="Ppp1r3a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCCGCC GAAGTACCCG GTCAGATTAG CAAAGATAAC TTTTTAGAAG 
     1081 TTCCTAATCT GTCCGATTCT GTGTGTGAAG ATGAAGAAGT TAAAGCGACC TTCAAGCCTG 
     1141 GCTTCTCCCC TCAGCCAAGC AGACGGGGGT CAGAATCTTC TGAAGACTTA TATCTGGACA 
     1201 CCCCAACCTC AGCTTCCCGG AGAGTTTCCT TTGCTGACAG CCTTGGATTC AGTCTTGTGT 
     1261 CTGTTAAAGA ATTTGATTGT TGGGACTTAC CAAGTGTTTC AACTGATTTT GACTTAACTA 
     1321 GGGATGTCCT CCACACAGAT GAATATATTT TATCTCCACT GTTTGACTTG CCTTCTTCCA 
     1381 AAGAAGAGCT TATGGAACAA CTTCAAGTCC AGAAGGCAGT GCTGGAGTCG GCTGAACACC 
     1441 TTCCTGGGTC GACAAGTGTG AAGGGCATCA TTCGAGTTCT GAACATTTCT TTTGAGAAGT 
     1501 TAGTGTATGT GCGCATGTCT TTGGACGACT GGCAGACACA TTATGACATT TTAGCAGAAT 
     1561 ATGTTCCTAA TTCGTGCGAT GGTGAAACTG ACCAGTTCTC CTTTAAGATT TCATTGGTTC 
     1621 CACCTTATCA AATGGACGGC GGTAAAGTGG AGTTTTGCAT ACGCTATGAA ACTTCTGTTG 
     1681 GTACATTTTG GTCAAATAAT AATGGGACAA ATTATATATT GGTTTGTCAA AAGAAAACAA 
     1741 AGGAGCCAGA GCCTGTAGTA AAGCCACTGG AAGAAGGTCC TAGTAGACAA ATAAAAGGCT 
     1801 GCTTAAAGGT AAAATCAAGC AGTAAAGAAG AACCATTGCT AACACCAGAA GAAAATAAGC 
     1861 TTGGGACTTT GAAGTTCACA GAGTCCTACA TCCCAACCAT TATTTGTTCT CATGAGGACA 
     1921 GGGATGACTT GGGAGCCAAT TATCAACATG TAAAAGACAT CGACAAGAAG CATGATGAAC 
     1981 ATAATGAAAA GGAATTAGAC TTGATGATAA ACCAACGGTT AATAACTTCC CATGACGAAA 
     2041 AGAATACATT TTCAACAGAT GCAGTCAACT CCACAAATAA AGCTGAGGGG TCAGAGAAGA 
     2101 AACAAGCTCG CCATGAAATC CACACTGACT TGTGCAGAGG GCCTTTGTCT CCGAGTTTAT 
     2161 CAGCAGAAAG TTCTTTAAAG AGAGATTTCT ACCACAGCAG AAATTATTCC CCAGGAAATG 
     2221 AGCATGGTCA TCCACCTTCA GAGGAAATTA TTTCGGATGT GGGAGCGATA GGGTCACCAC 
     2281 TGGGAGATAC TAGTTCTGAT GAGTTAATGC AACTGAAAGT CTGCAGCAAA GAAGACGTAG 
     2341 ACGATAATGC CAACCCAGCC CACGGGAGTG GCAGATTGTG TTCTTCCTCT GATCAACTAA 
     2401 GGGCAGGTGG TCTTGAGAAT AATGAAGCAG GAATCAAGAA AACTGGAATA AAAGATTACA 
     2461 AATATTCACA CGGAGATTCT TATTTAGAGG CATCCACAAG TTTGAGAGAA TCAAATGCGT 
     2521 CTTACAAAGA TGAGTACGAC AAAGTGGATG ATAAAAGGGA GAAGAAAACA TGCTTAGGTG 
     2581 TTAATGAAAA CCCAGGCAAG AATTTTCAAT CAATATTCCA GAACCAAGAG AGACAAATGG 
     2641 GGTGTGCAAA AATAAGCGGT GAAGGGGCTA ATGCTAATAA CCAAGACTTG ACCACTCTAC 
     2701 TGAGCAAAGA TACCACAACT AACACCTGGG CGGTAACCGT CGATCCCTCT CCTTCAACAA 
     2761 ATACAAACGT GAATTGGAGA GAAGCCGGAA ATGGTACCGA TTTAGAACGA AGAACTACTG 
     2821 ATCTTGGTTC TCCAAGGAAT TTCAGTCTCC TGACTGATGG TTATCTTTTT CAAGCTGACA 
     2881 GGGAAAAATC CGACTCTAGT AATTCTGAAG ATCGGAATAT GAACCCACAG CACAAAAAAA 
     2941 ATTGGAACGT TCTGGAAAGC CAGCCAGAAA CAAGAGAGAC TGAAACAAAT ATAACCAAGC 
     3001 ATACCAAAGA ACAGGCAGAG TACAAAGACA CGTGGGGAAA AAGAGATAAC AGTAGGAATC 
     3061 TGAAAGCTAC TTCTACAGAG CAATTGTTTA CGTGCCAAGA AACAGAATGC TGTGAACTGT 
     3121 CTTCTCTAGC TGATCATGGT ATCACTGAGA AAGCACAAGC AGTTGCAGCT TATATAATTA 
     3181 AGACAACATT AGAAAGTACT CCAGAAAGCG CGTCTGCTAG AGGAAAAGCA ATAATTGCTA 
     3241 AGCTACCTCA AGAGACAGCA CGACATGACA GGCCCATCGA GGTAAAAGAA ACAGCGTTTG 
     3301 ATCCACATGA AGGGCGGAAG GATGATTCAC ATTATTCCCT TTGTCATGGA GATACAGCAG 
     3361 GTGTAATCCA TGACAATGAT TTTGAAAGGG AGTCACATTT AGATATTTGT AATGTACGTG 
     3421 TAGATGAAAT GGAGAAGGAG AAAACCACAT CTACGTGCTC TCCTAGGAAG ACATATGACA 
     3481 AGGAGAAACA TGGAATTGGA AGTGTAACAT CTATAGACGA ACCTTCACAG GTCATTACAG 
     3541 GCAATCAAAA AGCTACGTCA AAACTGGATT TACATTTGGG AGTGTTACCA ACAGACAGAG 
     3601 CAATACTCTC GGCAAACGCA GATCTTGAGC TGCTTCAAGA ATTATCAAGG AGAACAGACT 
     3661 TCAATGCTGT TCACTCTGCA TTTAACTCAG ACCCTGGCAG CGCTTCTCAG GACAGCTCTC 
     3721 AAGTTTACAG ACGCTGCTCT AAAAAACCAG TACCTTCTTA TGGTGAACAG AAAGCAGTTA 
     3781 TTAACACAAC ACTCCAGTCT GTTCCTACGA AATCAGAATA TAATTGTCAC CCAGAAAGTG 
     3841 AGATCTTCGG TCATGCCACG TCTAAACCGG AAGTTGTTTT CAAAAGTTCA GAAATAATAA 
     3901 AATCAGGTAG TGGAGGAGAG CAAGGAGTGG GTCCGATTCT CCAACAAAAA GAAGGTAGTT 
     3961 TGGAAAAATC CCAAGACCCG ATGATTTCAA TCAACGAGCC TCTTGAGAAT CTGGATGAAG 
     4021 TACGTTCTGA AAATGAAGGG TTAATGCGTT CTGGTCAGTT ACAGTGTTAT TTGGGTGACA 
     4081 AGGGATCAGT GAGTTCTGCT TCTGCTACTG TCCCTACCCA GGAACTCCAA GCTCAAGGCA 
     4141 GCGAGTCTCT GCTTTCAATA TCCATCAACT CTAAAATACC TTATTTCCTT TTGTTTCTAA 
     4201 TATTTCTTGC AACTGTCTAC TACTATGACC TAATGATAGG CTTGGCATTC TATCTTTTTT 
     4261 CCTTGTACTG GTTGTACTGG GAAGGGGGGA GGCAAAGAGA GTCTGTCAAG AAAAAGACGC 
     4321 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4381 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4441 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4501 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4561 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4621 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4681 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4741 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4801 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4861 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4921 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4981 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5041 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5101 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5161 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5221 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5281 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5341 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5401 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5461 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5521 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5581 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5641 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5701 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5761 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5821 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5881 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5941 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6001 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6061 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6121 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6181 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6241 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6301 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6361 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6421 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6481 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6541 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6601 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6661 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6721 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6781 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6841 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6901 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6961 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7021 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7081 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7141 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7201 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7261 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7321 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7381 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7441 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7501 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7561 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7621 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7681 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7741 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7801 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7861 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7921 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7981 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8041 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8101 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8161 GCGAATTTT
//