LOCUS       RR208033                8100 bp    DNA     circular  SYN 
DEFINITION  Ube3b (Myc-DDK-tagged ORF) - Rat similar to ubiquitin protein ligase E3B (LOC687633), (10 ug)
ACCESSION   NM_001143894
VERSION     NM_001143894.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4255..4346 
                     /label="Myc-DDK"  
     misc_feature    4376..4961 
                     /label="PolyA signal"  
     misc_feature    4433..4452 
                     /label="Restriction Enzyme"  
     misc_feature    5237..5909 
                     /label="ColE1"  
     misc_feature    6339..7133 
                     /label="Kan/Neo"  
     misc_feature    7168..7470 
                     /label="SV40 ori"  
     misc_feature    7556..8033 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4230..4237 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4230 
                     /label="Ube3b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCACTGTA TCTCAGACCT CCAGAGCATG GTTCCTCGAC AAAGCCAGGC 
     1081 AGGCACGGGA AGAGAGGCTG GAGCAGAAGG AGCGGGAGCG CTCAGCAGTG CTGATCCAGG 
     1141 CACTAGTCCG CAGTTTCCTC TGTCGGAGGC GACTGCACAA AGATGTCAGG AGAGAGATCG 
     1201 ATGAGTTTTT CACTGCTGAT GAGTCTGGAT CCAGTAAAAG AAGTGCACTT TGTATTTTTA 
     1261 AGATTGCCAG GAAGCTGCTG TTCATATGCA AAACTACCGA GGACAATGAG AGAATGGAGA 
     1321 AGCTGTGTCG TAGCATCCTG AGCAGCATGG ATGCAGAGAA TGAGCCAAAG GTATGGTACG 
     1381 TGTCCCTGGC TCTTTCCAAG GACCTCACTC TGCTGTGGAT TAAACAGATC AAGAGCATCC 
     1441 TGTGGTACTG CTGTGAGCTT CTTGGGCAGC TCAAGCCAGA AATCTTACAG GACTCCAGGC 
     1501 TCATCACGCT GTACCTTACC ATGCTTGTCA CCTTCACGGA CACTTCAACA TGGAAGATTC 
     1561 TCCGGGGGAA AGGTGAAAGC CTTCGGCCAG CCCTGAACCA TATTTGTGCA AATATAATGG 
     1621 GCCACCTCAA CCAGCGAGGA TTCTACTCAG TGTTGCAGGT ACTGCTGACC CGAGGCCTGG 
     1681 CCAGACCCCG GCCCTGTCTG TCCAAAAGCA CGCTGACGGC TGCCTTCACT CTGGCCCTGC 
     1741 GCCCTGTAGT TGCTGCGCAG TTCTCAGACA GTCTGATGCG ACCGTTCATC ATCCACATCA 
     1801 TGTCGGTACC TGCTCTCGTG GCTCATCTCA GCACCATGGC CCCTGAGCGT CTCGGGGTTT 
     1861 TAGAGTCCCA TGACATGCTT CGTAAATTCA TCGTCTTTCT GAGAGACAGA GACCGATGTC 
     1921 AAGATGCTTG CGAAAGTTTA GAAGGCTGCC ATACCCTGTG CCTCATGGGC AATCTCCTGC 
     1981 ACTTGGGCTC CCTCAACCTT AAGATGTTAG AGGAAGAAAC AGATGGGTTT GTGAGCTTGC 
     2041 TCACCCAGAT GCTGTGCTAC TGTCAGAAGT ATGTGTCCCA GAAGAAGTCC AACCTGACCC 
     2101 ACTGGCACCC TGTGCTTGGC TGGTTTTCCC AGTCTGTGGA TTACGGCCTC AATGACTCCA 
     2161 TGTACCTGAT CACCAAGCAG CTGCAGTTCC TGTGGGGCGT GCCTCTCATC CGCATTTTCT 
     2221 TCAGTGACAT CCTCAGCAAG AAGCTGCTGG AGCCCCCAGA GCCAGTGCCT GCGCAGCCAC 
     2281 AGCCATCCTC CCCACAGACC GTGCTTCCCG TGAAGAGTCT CCTCAAGCGT GCGTTTCAAA 
     2341 AGTCAGCTTC AGTTCGGAAT ATCCTCAAGC CTGTCGGGGG CCGACGCGTG GACTCCACGG 
     2401 AGGTGCGGAA GGTCTGCAGC ATCTGTGTGC TCTACCAGAC CTCGCTGACA ACCCTCACCC 
     2461 AGATCCGCTT GCAGATCCTC ACAGGCCTTA CCTACCTTGA TGACCTGCTG CCCAAGCTCT 
     2521 GGGCTTTTAT CTGTGAGCTG GGGCCCCATG GAGGGCTAAA GCTCTTCTTG GAGTGCCTGA 
     2581 ACAATGACAC TGGAGAGTCC AAGCAGCTCC TGGCCATGCT GATGCTCTTC TGTGACTGCT 
     2641 CCCGCCACCT CATCACCATC CTGGACGACA TTGAAGTTTA TGAGGAGCAG ATCTCATTCA 
     2701 AGCTGGAAGA GCTGGTCACC ATCTCTTCCT TCCTCAACTC CTTTGTGTTC AAGATGATCT 
     2761 GGGATGGAAT CGTAGAGAAC GCCAAGGGTG AGACCTTGGA GCTGTTCCAG TCCGTCCATG 
     2821 GCTGGCTGAT GGTGCTGTAT GAACGAGACT GCCGCAGGCG CTTCGCTCCA GAGGACCACT 
     2881 GGCTACGCAA GGATCTCAAG CCTGGCGTGC TCTTCCAGGA ACTTGACAAG GACCGCAAGA 
     2941 GGGCTCAGCT GATCCTGCTG CACATTCCCC ACGTCGTGCC TCACAAGAAC AGAGTTCTTC 
     3001 TGTTCCGGAA CATGGTTATC AAGGAGAAAG AGAAGCTGGG GCTTGTGGAA ACCAGCTCTG 
     3061 CCTCCCCTCA CGTCACCCAC ATCACTATCC GAAGGTCCCG GATGCTGGAG GACGGCTATG 
     3121 AGCAACTGCG GCAGCTTCCT CAGCACGCCA TGAAGGGCGC GATCCGAGTG AAGTTCGTCA 
     3181 GTGACCTTGG GGTGGACGAA GCTGGCATTG ACCAGGACGG TGTCTTTAAG GAGTTTCTGG 
     3241 AAGAGATCAT CAAGAGGGTG TTTGACCCAG CACTCAACCT GTTCAAGACA ACCAGTGGGG 
     3301 ATGAGAGGCT GTACCCGTCA CCCACGTCCT ACATCCATGA GAACTACCTG CAGCTCTTTG 
     3361 AGTTTGTGGG GAAGATGCTG GGAAAGGCGG TGTATGAGGG GATCGTGGTG GACGTGCCCT 
     3421 TTGCCTCCTT CTTCCTGAGC CAAATGCTCG GGCACCACCA CAGCGTCTTC TACAGCTCCG 
     3481 TGGATGAGCT GCCCTCCTTG GACTCGGAGT TCTATAAAAA CCTCACCTCC ATCAAGCGCT 
     3541 ATGATGGAGA CGTCGCAGAC CTCGGGCTGA CACTGTCTTA CGATGAAGAT GTCATGGGTC 
     3601 AGCTCGTCTG CCACGAACTG GTTCCTGGAG GGAAGACCAT CCCGGTCACA GATGAGAACA 
     3661 AAATTAGCTA CATCCATCTG ATGGCTCATT TTCGAATGCA CACTCAAATC AAGAACCAGA 
     3721 CAGCTGCTCT CATTAGTGGC TTCCGTTCCA TTATTAAGCC CGAGTGGATC CGAATGTTCT 
     3781 CTACCCCTGA GCTTCAACGT CTCATCTCCG GGGACAATGC CGAGATCGAT CTGGAAGACT 
     3841 TAAAGAAGCA CACGGTGTAC TACGGTGGCT TTCACGGGAG TCACAGAGTC ATCGTCTGGC 
     3901 TCTGGGATAT CCTGGCTTCT GACTTCACGC CTGGTGAAAG AGCCATGTTC CTGAAGTTTG 
     3961 TGACCAGCTG CTCCCGACCC CCGCTCCTAG GATTCGCCTA CCTCAAACCT CCGTTCTCCA 
     4021 TTCGCTGTGT GGAAGTGTCG GATGACCAGG ACACTGGGGA CACCCTGGGC AGCGTCCTCA 
     4081 GGGGCTTCTT CACCATCCGC AAGAGGGAGC CAGGCGGGCG TCTGCCCACC TCTTCCACTT 
     4141 GCTTCAACCT GCTTAAGCTG CCCAACTACA GCAAGAAAAG CGTACTCCGA GAGAAACTGC 
     4201 GCTACGCCAT CAGCATGAAC ACAGGCTTTG AGCTCTCCAG CGGACCGACG CGTACGCGGC 
     4261 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4321 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4381 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4441 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4501 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4561 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4621 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4681 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4741 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4801 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4861 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4921 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4981 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5041 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5101 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5161 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5221 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5281 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5341 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5401 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5461 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5521 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5581 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5641 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5701 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5761 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5821 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5881 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5941 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6001 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6061 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6121 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6181 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6241 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6301 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6361 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6421 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6481 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6541 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6601 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6661 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6721 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6781 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6841 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6901 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6961 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7021 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7081 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7141 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7201 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7261 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7321 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7381 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7441 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7501 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7561 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7621 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7681 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7741 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7801 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7861 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7921 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7981 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8041 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//