LOCUS       RR208026                8085 bp    DNA     circular  SYN 
DEFINITION  Nfatc3 (Myc-DDK-tagged ORF) - Rat nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 (Nfatc3), (10 ug)
ACCESSION   NM_001108447
VERSION     NM_001108447.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4242..4333 
                     /label="Myc-DDK"  
     misc_feature    4363..4948 
                     /label="PolyA signal"  
     misc_feature    4420..4439 
                     /label="Restriction Enzyme"  
     misc_feature    5224..5896 
                     /label="ColE1"  
     misc_feature    6326..7120 
                     /label="Kan/Neo"  
     misc_feature    7155..7457 
                     /label="SV40 ori"  
     misc_feature    7543..8020 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4224..4230 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4224 
                     /label="Nfatc3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTACTGCA AACTGTGGCG CCCACGACGA GCTCGACTTT AAACTCGTCT 
     1081 TCGGCGAGGA CGGGGCACCA ACGCCGGTGT CCCAGGTCTC GCGGCCTGCA GATCTTGAAC 
     1141 CAGATGATTG TGCATCCATT TACATCTTTA ATGTAGACCC GCCTCCATCT ACTTTAAATT 
     1201 CATCACTTGG CTTACCACAT CATGGACTGC TGCAGTCTCA CTCTTCTGTT TTGTCACCAT 
     1261 CATTTCAGCT CCAAGGTTTC AAAAATTATG AAGGAACTGA TGATATTTCT GAATCTAAAT 
     1321 ATAGCTCATT AAGTGGTCCT AAACCCTTTG AATGCCCAAG TATTCAAATT ACATCCATCT 
     1381 CTCCTAACTG TCATCAAGAA ACAGATGCTC ATGAAGATGA CCTACATGTA AATGACCCAG 
     1441 AAAGGGAATA TTTGGAAAGG CCTTCTAGAG ATCATCTCTA TCTCCCACTT GAACCGTCCT 
     1501 ACCGGGAATC TTCCCTTAGT CCTAGTCCTG CCAGCAGCGT TTCTTCTAGG AGTTGGTTCT 
     1561 CAGATGCATC TTCTTGTGAG TCTCTCTCAC ACATTTATGA CGATGTGGAC TCAGAGTTGA 
     1621 ATGAAGCTGC TGCACGATTT ACTCTTGGCT CACCTCTGAC TTCTCCAGGT GGCTCTCCAG 
     1681 GAGGTTGCCC TGGAGAAGAG TCCTGGCATC AACAGTATGG ACCTGGACAT TCCTTGTCAC 
     1741 CTAGGCAATC TCCTTGCCAC TCTCCTAGAT CCAGTATCAC TGATGAGAAT TGGCTGAGCC 
     1801 CCAGACCAGC CTCAGGACCC TCATCCAGGC CCACTTCTCC TTGTGGTAAA CGACGGCACT 
     1861 CCAGTGCTGA AGTATGTTAT GCTGGCTCTC TTTCACCCCA TCACTCACCT GTTCCGTCCC 
     1921 CTGGTCACTC GCCTAGAGGG AGTGTAACTG AAGATACCTG GCTCACTGCT CCTGTCCACA 
     1981 CTGGATCAGG CCTCAGCCCT GCACCGTTTC CATTTCAGTA CTGTGTAGAG ACTGACATCC 
     2041 CTTTGAAAAC AAGGAAGACT TCTGACGATC AAGCTGCCAT ACTACCAGGA AAATTAGAGG 
     2101 TCTGTTCAGA TGATCAAGGG AGCTTATCCC CATCCCGGGA GACATCAGTA GATGATGGCC 
     2161 TTGGATCTCA GTATCCTTTA AAGAAAGATT CATCTGGTGA CCAATTTCTT TCAGTTCCTT 
     2221 CACCTTTTAC CTGGAGCAAA CCAAAGCCTG GCCACACTCC TATATTTCGC ACATCTTCAT 
     2281 TACCTCCATT AGACTGGCCT TTACCAACTC ACTTTGGACA ATGTGAATTG AAAATAGAAG 
     2341 TGCAACCTAA AACTCACCAT AGAGCCCATT ATGAAACTGA AGGTAGCCGA GGGGCAGTGA 
     2401 AAGCCTCTAC TGGTGGCCAT CCTGTTGTGA AGCTCCTGGG CTATAGTGAA AAGCCAATAA 
     2461 ATCTACAGAT GTTTATTGGA ACAGCCGATG ATAGATATTT ACGACCTCAC GCATTTTACC 
     2521 AGGTGCATCG CATTACTGGA AAGACAGTTG CTACTGCAAG TCAAGAGATA ATAATCGCCA 
     2581 GCACAAAAGT TCTGGAAATC CCACTTCTAC CTGAAAATAA TATGTCAGCA AGTATCGACT 
     2641 GTGCAGGTAT TTTGAAACTC CGCAATTCAG ATATAGAACT TCGAAAAGGA GAAACTGATA 
     2701 TTGGCAGAAA GAATACTAGA GTCCGACTTG TATTTCGTGT GCACATCCCA CAGCCCAGTG 
     2761 GAAAAGTCCT TTCTCTACAG ATAGCATCTA TTCCTGTTGA GTGCTCTCAG CGATCTGCTC 
     2821 AAGAGCTCCC TCACATTGAG AAGTACAGTA TCAACAGCTG CTCTGTCAAT GGAGGCCACG 
     2881 AAATGATTGT GACTGGATCT AATTTTCTTC CAGAATCCAA AATCATTTTT CTTGAAAAAG 
     2941 GACAAGATGG AAGACCTCAT TGGGAGGCTG AAGGAAAAAT AATCAGAGAA AAATGTCAAG 
     3001 GGGGTCATAT TGTCCTTGAA GTTCCTCCCT ATCATAACCC AGCAGTTACA TCTGCTGTGC 
     3061 AGGTGCACTT TTATCTTTGC AATGGCAAGA GGAAAAAAAG CCAGTCTCAA CGTTTTACTT 
     3121 ACACACCAGT TTTGATGAAG CAAGAACAAA GAGAAGATAC TGATTTGTCT TCAGTTCCAT 
     3181 CCTTGCCTGT GCCTCATTCT GCCCAGACCC AGAGGCCTTC CTCAGATACA GGGCACCCCC 
     3241 ATGACAGTGC ATTGTCTGCA CCAAGAAGCT TGATTTGTCC AGTGCAGCCA GCATATGCAT 
     3301 CTATGATAAC CTCCACCCAT TTGCCACAGT TGCAATGTAG GGATGAGGGT GCTGGCAAAG 
     3361 AACAGCACAT AATACCATCT TCGGTCATGC ACCAGCCTTT TCAAGTTACA CCAACATCTC 
     3421 CTATGGGGTC TTCCTATCAG TCTATACAAA CTAATATGTA TAATGGTCCA ACTTGTCTTC 
     3481 CTATGAATCC TGCCTCTAGT CAAGAATTTG ATCCAGTTTT GTTTCAGCAG GATGCAGCTC 
     3541 TTTCTAATTT AGTAAATCTT GGCTGTCAAC CACTGTCACC AATACCATTT CATTCTTCAA 
     3601 ATTCAGATGC AACAGGACAT CTTTTAGCTC ATTCACCTCA TTCTGTGCAA ACCCCACCTC 
     3661 ATCTGCAGTC AATGGGATAC CATTGTTCAA GTGCAGGACA AAGATCTCTT TCTTCTCCAG 
     3721 TAGCAGCCCA GGTCACAGGT CAGCCTTCTT CCCATTTACA GCCTATCACA TATTGTCCTT 
     3781 CACATCCAGG GTCTGCTACA GCAGCTTCCC CAGCAGCCTC TCATGCTCTT TCTAGTTCAC 
     3841 CGATTTCTGG GCCACCGTCC CCTCAGCTGC AGTCTATGCC TTACCAATCT CCTAGCTCAG 
     3901 GAACTGCCTC ATCACCATCT CCAGTTACCA GAATGCATTC TGGACAGCAC TCAACTCAAG 
     3961 CACAAAGTAC AGGGCAAGGA GGTCTTTCTG TACCTTCATC CTTAGTTTGT CACAGTTTGT 
     4021 GTGACCCAGC ATCGTTTCCA CCTGGTGGTG CAGCTGTGAG CATTAAACCT GAACCTGAAG 
     4081 ATCAAGAACC TAACTTTGCA ACCATTGGTC TACAGGATAT CACTTTAGAT GATGACCAGT 
     4141 TTATGTCTGA CTTGGAACAC CAGCCATCAG GTTCAACAGA GAAATGGCCT AATCACAGTG 
     4201 AGTTCTCATG TCCAGTTCCT TTCTGGCGAA TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4261 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4321 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4381 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4441 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4501 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4561 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4621 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4681 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4741 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4801 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4861 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4921 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4981 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5041 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5101 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5161 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5221 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5281 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5341 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5401 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5461 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5521 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5581 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5641 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5701 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5761 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5821 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5881 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5941 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6001 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6061 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6121 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6181 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6241 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6301 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6361 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6421 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6481 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6541 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6601 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6661 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6721 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6781 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6841 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6901 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6961 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7021 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7081 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7141 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7201 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7261 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7321 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7381 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7441 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7501 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7561 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7621 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7681 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7741 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7801 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7861 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7921 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7981 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8041 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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