LOCUS       RR208024                6134 bp    DNA     circular  SYN 
DEFINITION  Txnip (Myc-DDK-tagged ORF) - Rat thioredoxin interacting protein (Txnip), (10 ug)
ACCESSION   NM_001008767
VERSION     NM_001008767.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2289..2380 
                     /label="Myc-DDK"  
     misc_feature    2410..2995 
                     /label="PolyA signal"  
     misc_feature    2467..2486 
                     /label="Restriction Enzyme"  
     misc_feature    3271..3943 
                     /label="ColE1"  
     misc_feature    4373..5167 
                     /label="Kan/Neo"  
     misc_feature    5202..5504 
                     /label="SV40 ori"  
     misc_feature    5590..6067 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2264..2271 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2264 
                     /label="Txnip"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGATGTTC AAGAAGATCA AGTCTTTTGA GGTGGTCTTC AACGACCCTG 
     1081 AGAAGGTGTA TGGCAGCGGG GAGAAGGTGG CCGGCCGGGT GACAGTGGAA GTGTGTGAAG 
     1141 TTACCCGAGT CAAAGCCGTC AGGATCCTGG CTTGCGGTGT GGCCAAGGTC CTGTGGATGC 
     1201 AAGGGTCTCA GCAGTGCAAA CAGACCTTGG ACTACTTGCG CTATGAAGAC ACCCTTCTCC 
     1261 TAGAAGACCA GCCTACAGGT GAGAACGAGA TGGTGATCAT GAGACCAGGA AACAAATATG 
     1321 AGTACAAGTT CGGCTTTGAG CTTCCTCAAG GGCCCCTGGG AACATCCTTC AAAGGGAAAT 
     1381 ATGGCTGTGT AGACTACTGG GTGAAGGCTT TTCTCGATCG TCCCAGCCAG CCAACTCAAG 
     1441 AGGCAAAGAA AAACTTTGAA GTGATGGATC TAGTGGACGT CAATACTCCT GACTTAATGG 
     1501 CACCAGTGTC TGCCAAAAAG GAGAAGAAAG TTTCCTGCAT GTTCATTCCT GATGGACGCG 
     1561 TGTCAGTCTC CGCTCGAATC GACAGAAAAG GATTCTGTGA AGGTGATGAC ATTTCCATCC 
     1621 ACGCTGACTT TGAGAACACA TGTTCCCGAA TCGTGGTCCC CAAAGCAGCT ATTGTGGCCC 
     1681 GGCACACTTA CCTTGCCAAT GGCCAGACCA AAGTGCTCAC TCAGAAGCTG TCCTCAGTCA 
     1741 GAGGCAATCA CATTATCTCA GGGACTTGCG CATCATGGCG TGGCAAGAGT CTCCGAGTGC 
     1801 AGAAGATCAG ACCGTCCATT CTGGGCTGCA ACATCCTCAG AGTTGAATAC TCCTTGCTGA 
     1861 TCTATGTTAG TGTCCCTGGC TCCAAGAAAG TCATCCTCGA TCTGCCCCTA GTGATTGGCA 
     1921 GCAGGTCGGG CCTAAGCAGC CGGACATCCA GCATGGCTAG CCGGACAAGC TCTGAAATGA 
     1981 GCTGGATAGA CCTAAACATC CCAGACACCC CAGAAGCTCC ACCTTGCTAT ATGGACGTGA 
     2041 TTCCTGAAGA TCACAGACTG GAGAGCCCCA CCACCCCCCT GCTAGATGAT GTGGATGATT 
     2101 CTCAAGACAG CCCTATCTTT ATGTACGCCC CCGAGTTCCA GTTCATGCCT CCGCCCACTT 
     2161 ACACTGAGGT GGATCCCTGC GTCCTTAACA ACAACAACAA CAACGTGCAG CTCCGCCCAC 
     2221 TTACACTGAG GTGGATCCCT GCGTCCTTAA CAACAACAAC AACAACGTGC AGAGCGGACC 
     2281 GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA 
     2341 TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG 
     2401 TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC 
     2461 TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT 
     2521 TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG 
     2581 GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG 
     2641 CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC 
     2701 CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT 
     2761 TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC 
     2821 AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC 
     2881 CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC 
     2941 ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC 
     3001 TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA 
     3061 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 
     3121 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 
     3181 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 
     3241 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 
     3301 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 
     3361 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 
     3421 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 
     3481 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 
     3541 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 
     3601 TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG 
     3661 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 
     3721 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 
     3781 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 
     3841 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 
     3901 AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC 
     3961 CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG 
     4021 GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG 
     4081 CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT 
     4141 CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT 
     4201 AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC 
     4261 CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA 
     4321 TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC 
     4381 CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG 
     4441 CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT 
     4501 CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA 
     4561 TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG 
     4621 TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG 
     4681 GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC 
     4741 GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT 
     4801 CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA 
     4861 GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG 
     4921 CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA 
     4981 GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA 
     5041 GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT 
     5101 GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA 
     5161 ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA 
     5221 AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG 
     5281 GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA 
     5341 CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA 
     5401 TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG 
     5461 GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC 
     5521 TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC 
     5581 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     5641 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     5701 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 
     5761 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
     5821 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 
     5881 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 
     5941 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 
     6001 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 
     6061 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
     6121 TTAACGCGAA TTTT
//