LOCUS       RR208009                8292 bp    DNA     circular  SYN 
DEFINITION  Tie1 (Myc-DDK-tagged ORF) - Rat tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), (10 ug)
ACCESSION   NM_053545
VERSION     NM_053545.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4449..4540 
                     /label="Myc-DDK"  
     misc_feature    4570..5155 
                     /label="PolyA signal"  
     misc_feature    4627..4646 
                     /label="Restriction Enzyme"  
     misc_feature    5431..6103 
                     /label="ColE1"  
     misc_feature    6533..7327 
                     /label="Kan/Neo"  
     misc_feature    7362..7664 
                     /label="SV40 ori"  
     misc_feature    7750..8227 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4431..4437 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4431 
                     /label="Tie1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCTGGTGG GGATCCCCTT TCCTGCTCCC CATTCTTTTT CTGGCCTCTC 
     1081 ATGTTGGTGC ATCCGTGGAT TTAACACTGC TGGCCAATCT GCGTATCACC GAACCCCAGC 
     1141 GTTTCTTCCT GACCTGTGTG TCTGGTGAGG CGGGAGCAGG GAGGAGCTCC GACGTCTGGG 
     1201 GCCCGCCCCT GCTGCTGGAA AAGGATGATC GCATAGTGCG CACCTTCCCA CCCGGGCAGC 
     1261 CCCTGCACCT CACTCGCAAC GGTTCACACC AGGTCACGCT GCGGGGCTTC TCCAAGCCCT 
     1321 CGGATTTAGT AGGCGTCTTC TCCTGCGTGG GTGGAGCTGG CACGAGGCGC ACTCGAGTCC 
     1381 TCTATGTGCA CAACAGCCCG GGGGCACACC TGTTTCCAGA CAAGGTCACA CACACGGTGA 
     1441 ACAAAGGTGA TACCGCTGTG CTTTCTGCAC GAGTGCACAA GGAAAAGCAG ACTGATGTGA 
     1501 TTTGGAAGAA CAATGGATCC TACTTCCATA CCCTAGACTG GCATGAGGCC CATGATGGGC 
     1561 GGTTTCAACT GCAGCTCCAA AATGTACAGC CACCATCGAG TGGCATCTAC AGCGCCACCT 
     1621 ACCTAGAGGC CAGCCCCTTG GGCAGTGCCT TCTTTCGGCT CATCGTGCGA GGCTGTGAGG 
     1681 CTGGACGCTG GGGGCCGGGA TGTGTCAAGG ATTGCCCAGG CTGCCTGCAT GGAGGTGTCT 
     1741 GTCATGACCA CGATGGCGAA TGTGTGTGCC CCCCTGGATT CACTGGCACC CGCTGTGAGC 
     1801 AGGCCTGCAG AGAAGGTCGT TTTGGACAGA GCTGCCAGGA ACAGTGCCCA GGCACAGCAG 
     1861 GCTGTCGGGG TCTCACCTTC TGCCTCCCAG ATCCCTATGG CTGTTCTTGT GGATCTGGCT 
     1921 GGAGGGGAAG CCAGTGCCAG GAAGCATGTG CACCTGGTCA TTTTGGGGCA GATTGTCGCC 
     1981 TCCAGTGTCA GTGTCAAAAT GGTGGTACTT GTGATCGGTT CAGCGGCTGC GTCTGCCCCT 
     2041 CCGGGTGGCA TGGAGTCCAC TGTGAAAAGT CAGACCGGAT CCCCCAGATC CTCAATGTGG 
     2101 CCACAGAGCT GGAGTTCAAC TTAGGGACAA TGCCCCGGAT CAACTGTGCA GCTGCCGGGA 
     2161 ATCCCTTCCC AGTACGGGGC AGCATGGAAC TCCGCAAGCC AGACGGCACC ATGCTCCTGT 
     2221 CTACCAAAGC CATTGTGGAG CCAGATAGGA CCACAGCTGA GTTCGAGGTG CCCCGTTTGA 
     2281 CTCTTGGGGA CAGCGGGTTC TGGGAATGCC GTGTATCGAC GTCTGGTGGC CAAGATAGCC 
     2341 GGCGCTTCAA GGTCAATGTC AAAGTACCCC CAGTGCCTCT GACTGCACCT CGGCTCCTGG 
     2401 CCAAGCAGAG CCGCCAGCTT GTGGTCTCCC CACTGGTCTC CTTCGGTGGG GATGGACCCA 
     2461 TCTCCTCTGT CCGCCTGCAC TACCGGCCCC AGGACAGCAT GATTACCTGG TCTGCCATTG 
     2521 TGGTGGATCC CAGTGAGAAT GTGACATTAA TGAACCTGAA GCCAAGGACA GGATACAACG 
     2581 TTCGGGTGCA GCTGAGCCGG CCAGGGGAAG GAGGAGAAGG AGCCTGGGGT CCTTCGACTC 
     2641 TCATGACTAC GGACTGTCCT GAGCCTTTGC TGCAGCCGTG GGTAGAGAGC TGGAACGTGG 
     2701 AGGGTCCTGA CAGGCTACGG GTGAGCTGGT CTCTACCCTC GGTGCCACTG TCTGGGGATG 
     2761 GTTTCCTGCT GCGTCTGTGG GATGGGGCCC GGGGACAGGA GAGGCGGGAG AACATCTCAT 
     2821 CCCCCCAGGC CCGCACTGCC CTCCTGACTG GACTCACGCC TGGCACGCAC TACCAACTGG 
     2881 ATGTGCGGCT GTATCACTGC ACCCTCCTGG GCCCTGCCTC ACCCTCTGCG CATGTGCACC 
     2941 TGCCCCTCAG CGGGCCTCCA GCTCCCCGCC ACCTCCGTGC CCAGGCCCTC TCAGACTCTG 
     3001 AGATCCGTCT GATGTGGCAG CATCCTGAGG CTCCGCCTGG ACCTATATCC AAGTACATTG 
     3061 TGGAGATTCA GGTGGCCGGG GGCTCAGGAG ACCCTCAGTG GATGGATGTA GACAAACCCG 
     3121 AGGAGACGAG CACCACCGTC CGTGGTCTCA ATGCCAGCAC ACGCTACCTC TTCCGTGTGC 
     3181 GGGCCAGTGT GCAGGGTCTC GGCGACTGGA GCAACACAGT GGAAGAGACC ACGCTGGGTA 
     3241 ATGGGCTGCA GAGTGCGAGC CCAGTCCAAG AGAGCCGGGT GGCTGAAGAC GGTCTGGATC 
     3301 AGCAGCTGGT CCTGGCTGTG GTAGGCTCCG TCTCTGCCAC CTGCCTCACC ATCCTGGCTG 
     3361 CCCTTTTAGC CCTGGTGTGT ATCCGAAGAA GCTGCCTACA TCGGAGACAT ACCTTCACCT 
     3421 ACCAGTCAGG ATCGGGTGAA GAGACCATCC TGCAGTTCAG CTCAGGGACC TTGACATTGA 
     3481 CCCGACGGCC AAAGCCACAG CCTGAGCCTT TGAGTTACCC TGTGCTGGAG TGGGAGGACA 
     3541 TCACCTTTGA GGACCTCATC GGGGAGGGGA ACTTCGGCCA AGTGATCCGG GCCATGATCA 
     3601 AGAAGGACGG ACTCAAGATG AACGCAGCCA TCAAGATGCT GAAAGAGTAT GCGTCTGAAA 
     3661 ATGACCACCG AGACTTTGCA GGGGAACTAG AAGTTCTGTG CAAACTAGGA CACCACCCCA 
     3721 ATATTATCAA CCTCTTGGGG GCCTGTGAGA ACCGAGGCTA CTTGTATATT GCGATCGAAT 
     3781 ATGCCCCGTA TGGAAACCTG CTAGATTTCC TGAGAAAGAG CAGGGTCCTG GAGACTGATC 
     3841 CAGCTTTTGC TCGAGAGCAT GGAACAGCCT CCACACTCAG CTCCCGGCAG CTGCTGCGCT 
     3901 TTGCCAGTGA TGCAGCCAAC GGGATGCAGT ACCTTAGTGA GAAGCAGTTC ATTCACAGGG 
     3961 ACCTGGCTGC CCGAAATGTT CTGGTCGGAG AGAACCTGGC CTCCAAGATT GCAGACTTTG 
     4021 GCCTTTCTCG GGGGGAGGAA GTATATGTGA AGAAGACGAT GGGCCGTCTT CCTGTGCGTT 
     4081 GGATGGCCAT TGAGTCCCTC AACTACAGCG TCTACACTAC CAAGAGTGAC GTCTGGTCAT 
     4141 TTGGGGTCCT CCTCTGGGAG ATAGTGAGCC TTGGAGGCAC GCCCTACTGT GGCATGACCT 
     4201 GTGCTGAGCT CTACGAGAAA CTGCCTCAAG GCTATCGCAT GGAGCAGCCT CGAAACTGTG 
     4261 ATGATGAAGT GTACGAGCTG ATGCGGCAGT GCTGGCGGGA CCGTCCCTAT GAGCGCCCCC 
     4321 CTTTTGCCCA GATTGCACTA CAGTTGGGCC GCATGCTGGA AGCCAGGAAG GCCTACGTGA 
     4381 ACATGTCACT GTTTGAGAAC TTTACCTATG CAGGCATCGA TGCCACAGCT GAGGAGGCCA 
     4441 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4501 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4561 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4621 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4681 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4741 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4801 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4861 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4921 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4981 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5041 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5101 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5161 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5221 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5281 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5341 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5401 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5461 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5521 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5581 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5641 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5701 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5761 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5821 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5881 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5941 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6001 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6061 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6121 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6181 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6241 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6301 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6361 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6421 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6481 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6541 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6601 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6661 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6721 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6781 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6841 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6901 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6961 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7021 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7081 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7141 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7201 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7261 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7321 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7381 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7441 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7501 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7561 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7741 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7801 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7861 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7921 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7981 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8041 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8101 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8161 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8221 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8281 AACGCGAATT TT
//