LOCUS       RR207994                9165 bp    DNA     circular  SYN 
DEFINITION  Nphp4 (Myc-DDK-tagged ORF) - Rat nephronophthisis 4 (juvenile) homolog (human) (Nphp4), (10 ug)
ACCESSION   NM_001037650
VERSION     NM_001037650.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5322..5413 
                     /label="Myc-DDK"  
     misc_feature    5443..6028 
                     /label="PolyA signal"  
     misc_feature    5500..5519 
                     /label="Restriction Enzyme"  
     misc_feature    6304..6976 
                     /label="ColE1"  
     misc_feature    7406..8200 
                     /label="Kan/Neo"  
     misc_feature    8235..8537 
                     /label="SV40 ori"  
     misc_feature    8623..9100 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5304..5310 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5304 
                     /label="Nphp4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGACTGG CACAGAGCCT TCACCCAGAA CACACTGGTC CCTCCCCACC 
     1081 CGCAGAGAGC GCGGCAGTTT GGGAAGGAGT CCACGGCGTT CCAGTGCATC CTGAAGTGGC 
     1141 TTGATGGGCC ACTCATTAAG CAGGGAATCC TAGACATGCT GTCTGAGCTT GAATGCCACC 
     1201 TGCGAGTGTC TCTCTTCGAC GTCACCTACA AACATTTCTT CGGGAGGACG TGGAAAACCA 
     1261 CAGTGAAGCC CACCAATCAG CTCTCTAAGC AGCCACCCAG GATCCTCTTC AATGAGCCCT 
     1321 TGTACTTCCA CACAACCTTG AGCCACCCCA GCATCGTGGC TGTAATAGAA GTGGTCGCTG 
     1381 AAGGCAGGAA GAGGGACGGT GCCCTGCAGG TGCTGTCCTG TGGGTTTGGA ATTCTTCGAA 
     1441 TTTTTGGCAA TAAGCCGGAA TCTCCCACCT CTGCAGGCCA GGACAAACGG TTGAGGCTGT 
     1501 ATCATGGTAC CCCCAGAGCC CTTCTGCACC CACTTCTCCA GGATCCCATA GAACAGAACA 
     1561 GGTACATGAC AATGATGGAA AACTGTAGCC TGCAGTATAC CCTGAAGCCA CACCCGCCCC 
     1621 TGGAGCCCGC CTTCCACCTG CTCCCCGAGA ACTTCCTGAT CTCCGGTCTC CAGCAGATCC 
     1681 CTGGCCTCCT CCCACCTCAT GGAGACACGG GTGATGCCCT GAGGAAGCCT CGCTTCCAGA 
     1741 AGCCCACCAC ATTGCACCTA GATGACCTCT TCTTCACCCT GTACCCCTCC CTGGAGAAGT 
     1801 TTGAGGAGGA ACTTGTGCAG CTGCTCATCA GCGACCACTT CCGGGAGGGG GTTGGCCTGC 
     1861 TAGATAGCGG CACCCTAGAG GTCTTGGAGC GGCGCCTGCA TGTCTGTGTC CACAACGGTC 
     1921 TGGGCTTCGT GCACAGGCCA CAGGTGGTTG TGCTTGTACC TGAGATGGAC ATGACCTTGA 
     1981 CGCGCTCGGC CAGCTTCAGC AGGAGAATCA CTGCCTCTTC TAAGAGCAGC TCTGGAAATC 
     2041 AGGCTCTGGT TTTGAGAAGC CACCTGAGAC TCCCGGAGAT GGTCAATCAC CCATCGTTTG 
     2101 CCATCGTCTT CCAGCTGGAG TACGTGTTCA ACAGCCCCTC AGGAGCGGAC GGCAGTGCCA 
     2161 GCTCATCCAC CTCTCTGTCC AGCTTGGCGT GCATGCACAT GGTTCGATGG GCTGTTTGGA 
     2221 ACCCCGATCT GGAAGCAGGC CCGGGAAAGG TGACGCTGCC TCTTCAGGGT GGGGTACAGC 
     2281 ACAACCCCTC ACGATGTCTG GTCTATAAGG TGCCTTCGGC TAGCATGAGC TCTGAGGAGG 
     2341 TGAAGCAGGT GGAATCCGGA ACCATCCAGT TCCACTTCTC ACTGAGCTCA GATGCCCTTA 
     2401 CAGAGCATGC CAACGGCCCC AGGGCAGGGC GGCGCTCCAC CAGAAAGGCA CTAACGTCCC 
     2461 CTTCAGGCAC ACCAGCACTG GCAGCCCGGG ATCTTGCTGC GATACAGGAC TCTCCTGTGG 
     2521 GCCCAGGGCT CTCGCTCTCC CAGCTGGCGG CCTCCCCACA GTCTCTGGCC TCACAAAGAT 
     2581 CTTTCAAGCC TCCTCCTCAG CCCCTGGATG GCTCTCAGTC CCCAGAGGGT CCCCAGCTCC 
     2641 AAGCAGGATC TGTATTAGAG AGCAGGATCT CTCATCTGGA AGCTGACCTG AGCCAGCCGC 
     2701 CCTTCTCGGT GCAGGGGACG CCCACTGTAG AACACCTACA GGAACTTCCC TTCACTCCTT 
     2761 TGCATGCCCC CATTGTGGTG GGAGCCCAGA CCAGGAGCTC CAGAAGCCAG CTCTCCAGAG 
     2821 CTGCCATGGT CCTCCTGCAG TCTTCCGGCT TCCCTGAGAT CCTTGATGCC AGGCAGCAGC 
     2881 CGGTGGAGGC CGTCAATCCC ATGGATCCTG TGAGGTTCAA CCCTCAGAAG GAGGAGTCAG 
     2941 ACTGCCTTCT AGGGAATGAG ATCGTGCTGC AGTTTCTTGC CTTCAGCAGA GCGGCCCAGG 
     3001 ACTGCCCGGG AGCGCCGTGG CCACAGACTG TGTATTTCAC CTTCCAGTTC TACCGTTTCC 
     3061 CACCCGAGAC CACGCCACGC CTGCAGCTGG TCAAACTGGA CAGGACAGGC AAGAACGGCT 
     3121 CTGCCTCCCT GTCTCACATC CTGGTCCACA TCAATAAAGA CGGCTCCTTT GACGCTGGCT 
     3181 CCCCTGGCTT CCAGCTGAGG TACATGGTGG ATCCCGGGTT CCTGAAGCCA GGAGAGCAGC 
     3241 GCTGGTTCGC CCAGTACCTG GCCGCTCAGA CGCTGCAAGT CGACGTCTGG GATGGAGACT 
     3301 CCTTGCTCCT CATTGGGTCT GCCGGCATCC AGATGAAGCA CCTCCTCCGC CAAGGGCGGC 
     3361 CAGCTGTTCA GGTCTCACAT GAACTGGAGG TCGTGGCCAC TGAGTACGAA CAGGAAATGA 
     3421 TGGTGGTGAG CGGCGACGTG GCGGGCTTCG GCAGCGTCAA GCCCATCGGA GTCCACACGG 
     3481 TCGTGAAGGG CCGGCTGCAC CTGACCTTGG CCAACGTGGG TCACGAGTGT GAACCGAGAG 
     3541 CAAGAGGTTC CAACGCGCTG CCACCATCCA GGTCCCGGGT CATCTCAAAT AATGGAGCCA 
     3601 GCTTCTTCTC TGGGGGAAGC CTCCTCATAC CAGGAGGCCC CAAGCGAAAG CGTGTGGTTC 
     3661 AAGCCCAGAA GCTGGCTGAT GTGGACAGTG AGCTGGCCGC CATGCTGCTG ACCCACACTC 
     3721 GAGCTGGCCA GGGGCCCCAG GCTGTGGGCC AGGAGGCAGA CGCTGTGCAC AGGCGCAAGC 
     3781 TGGAAAGGAT GCGGCTGGTG CGCCTGCAGG AGGCCGGAGG GGACTCTGAC AGTCGCAGGA 
     3841 TCAGCGTGCT GGCCCGACAC AGTGTCCGTG CACAGCACTC ACGGGACCTG CAGGTCATCG 
     3901 ACGCCTACCG GGAACGGACC AAGGCTGAGA ACATTGCCAG CGTGTTGAGC CAGGCCATCA 
     3961 CCACACACCA CACACTGTAT GCCACGCTGG GCACGGCTGA GTTCTTCGAG TTTGCACTTA 
     4021 AAAACCCACA CAACACTCAG CACACGGTGG CCATCGAGAT TGACAGCCCT GAGCTCAGCA 
     4081 TCATCCTGGA CAGCCAGGAG TGGAGGTACT TCAAGGAGGC CACTGGCCTG CTCACACCCC 
     4141 TGGAGGAGGA CATGTTCCAC CTGCGTGGGA GCCTGGCACC CCAGCTCTAC CTGCGGCCCC 
     4201 GGGAGACTGC CCACATCCCC TTCAAGTTCC AGAACTTCTC TGTGGGCCCA CCTGCTCCGA 
     4261 CACAGGCCCC TGCGGAGGTG ATCTCAGAGA AGGATGCAGA GTCTGGACCC CATTGGAAGT 
     4321 GCAGTGCCAT GCCTACCAAA CACGCCAAGG TCCTTTTCCG GGTGGAGACC GGCCAGCTCA 
     4381 TTGCTGTGCT CTGCCTGACC GTGGAGCCCC AGCCCCACGT GGTGGACCAG GTTTTCCGTT 
     4441 TCTACCACCC AGAACTCACC TTCCTGAAGA AGGCCATCCG CCTGCCGCCC TGGCACACGC 
     4501 TTCCAGGTGC TCCCGTGGGG ATGCCTGGCG AGGACCCCCC AGTCCACGTT CGATGCAGCG 
     4561 ACCCGAATGT CATCTGCGAG GCCCAGAACG TGGGCCTTGG TGAGCCGAGG GACGTGTTCC 
     4621 TTAAGGTGGC CAGCGGTCCA AGCCCGGAGA TCAAAGACTT CTTTGTCGTC ATCTATGCGG 
     4681 ATCGCTGGCT GGCGGTGCCC GTGCAGACAT GGCAGGTCTG CCTCCACTCC CTGCAGCGCG 
     4741 TGGATGTCTC CTGTGTCGCA GGGCAGATGA CCCGTCTGTC CCTCGTCCTA CGGGGGACGC 
     4801 AGACCGTTAG GAAAGTGAGA GCTTTCACCT CCCACCCTCA GGAGCTGAAG ACAGACCCCG 
     4861 CTGGTGTCTT TGTGTTACCA CCTCACGGGG TGCAGGACCT GCATGTGGGC GTGAGGCCAA 
     4921 GGAGGGCGGG CAGCCGCTTC ATCCATCTCA ACCTGGTGGA CGTCGACTAC CACCAGCTGG 
     4981 TCGCGTCGTG GCTCGTGTGC CTGTCCTGCC GCCAGCCCCT CATTTCCAAG GCCTTCGAGA 
     5041 TCACCATGGC AGCAGGCGAA GGGAAGGGTG CCAACAAGCG GATCACCTAC ACCAACCCCT 
     5101 ACCCTTCTCG GAGGACCTAC CGTCTGCACA GTGACCGGCC AGACCTGCTG CATTTCAAGG 
     5161 AGGACTCCTT CCAGGTGGCC GGAGGGGAGA CCTACACCAT CGGGCTGCGG TTCTTGCCGA 
     5221 GTGGCAGCGC CGGGCAGGAG GAGATCCTGA TCTACATCAA TGACCAGGAC GACAAGAATG 
     5281 AAGAGACATT CTGTGTGAAG GTCCTCTACC AGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5341 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5401 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5461 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5521 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5581 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5641 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5701 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5761 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5821 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5881 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5941 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     6001 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6061 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6121 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6181 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6241 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6301 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6361 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6421 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6481 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6541 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6601 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6661 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6721 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6781 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6841 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6901 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6961 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     7021 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7081 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7141 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7201 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7261 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7321 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7381 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7441 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7501 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7561 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7621 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7681 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7741 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7801 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7861 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7921 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7981 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     8041 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8101 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8161 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8221 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8281 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8341 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8401 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8461 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8521 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8581 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8641 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8701 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8761 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8821 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8881 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8941 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     9001 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9061 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9121 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//