LOCUS       RR207982                8703 bp    DNA     circular  SYN 
DEFINITION  Mybpc3 (Myc-DDK-tagged ORF) - Rat myosin binding protein C, cardiac (Mybpc3), (10 ug)
ACCESSION   NM_001106490
VERSION     NM_001106490.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4860..4951 
                     /label="Myc-DDK"  
     misc_feature    4981..5566 
                     /label="PolyA signal"  
     misc_feature    5038..5057 
                     /label="Restriction Enzyme"  
     misc_feature    5842..6514 
                     /label="ColE1"  
     misc_feature    6944..7738 
                     /label="Kan/Neo"  
     misc_feature    7773..8075 
                     /label="SV40 ori"  
     misc_feature    8161..8638 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4842..4848 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4842 
                     /label="Mybpc3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGAGCCA GGGAAGAGAC CAGTGTCAGC CTTCACCAAG AAGCCCAGGT 
     1081 CAGTGGAGGT GACCGCAGGC AGTGCTGCTG TGTTCGAGGC TGAGACGGAG CGGTCAGGCC 
     1141 TGAAGGTGCA GTGGCAGCGG GATGGCAGTG ATATCGCTGC CAATGACAAG TATGGTTTGG 
     1201 CAGCAGAGGG CAAACGACAC ACACTGACAG TGCGGGATGT GGGCCCTGAC GATCAGGGTT 
     1261 CCTATGCGGT CATCGCAGGC TCCTCAAAGG TCAAGTTTGA CCTTAAGGTC ACAGAGCCAG 
     1321 CCCCTCCAGA GAAGGCAGAG TCTGCAGTTG CTCCAACCTC TATGGAAGCT CCAGAAACTC 
     1381 CCAAAGAAGT TCCTGCTCTA GCCACTCAGT TGGAAGGAAA TGTCTCAAGT CCTGAAGGGT 
     1441 CAGTCTCGGT AACCCAGGAT GGCTCAGTCG CAGGGAGTCA GGGAGCCCCT GACGACCCTA 
     1501 TTGGCCTCTT TCTGATGCGG CCACAGGATG GCGAGGTGAC CGTGGGCGGC AGCATCGTCT 
     1561 TCTCAGCCCG AGTGGCGGGA GCCAGCCTCC TGAAACCGCC TGTAGTCAAG TGGTTTAAGG 
     1621 GCAAGTGGGT GGACCTGAGC AGCAAGGTGG GCCAGCACCT GCAGCTGCAC GACAGCTACG 
     1681 ACAGAGCCAG CAAGGTCTAC TTGTTTGAGT TGCACATCAC AGATGCGCAG GCCACTTCTG 
     1741 CTGGGGGCTA CCGCTGTGAG GTGTCTACCA AGGACAAATT TGACAGCTGC AACTTCAACC 
     1801 TCACTGTACA TGAGGCCATT GGTTCTGGAG ACCTGGACCT CAGATCAGCT TTCCGACGCA 
     1861 CGAGCCTGGC TGGAACGGGT CGGAGAACCA GTGACAGCCA TGAAGATGCT GGGACTCTGG 
     1921 ACTTTAGCTC CCTGCTGAAG AAGAGCAGTT TCCGGAGGGA CTCAAAGCTA GAAGCACCCG 
     1981 CTGAAGAAGA TGTGTGGGAG ATCCTGAGAC AGGCGCCACC GTCAGAATAT GAGCGCATCG 
     2041 CCTTCCAGCA CGGAGTCACA GACCTGCGAG GCATGCTGAA GAGGCTCAAG GGCATGAAGC 
     2101 ACGATGAAAA GAAGAGCACA GCCTTTCAGA AGAAGCTGGA GCCTGCCTAC CAGGTAAACA 
     2161 AGGGCCACAA GATCCGGCTT ACTGTGGAAC TGGCTGATCC AGACGCCGAA GTCAAGTGGC 
     2221 TTAAGAATGG ACAGGAGATC CAGATGAGTG GCAGGTACAT CTTTGAGTCC ATCGGTGCCA 
     2281 AGCGTACCCT GACCATCAGC CAGTGCTCAC TGGCTGACGA TGCAGCCTAC CAGTGTGTGG 
     2341 TGGGGGGTGA GAAATGCAGC ACAGAGCTCT TTGTCAAAGA ACCCCCGGTG CTGATCACTC 
     2401 GGTCCCTGGA AGACCAGCTA GTGATGGTGG GTCAGCGGGT GGAGTTTGAG TGTGAGGTCT 
     2461 CAGAAGAAGG GGCCCAAGTC AAATGGCTGA AGGATGGGGT TGAGCTGACT CGGGAGGAGA 
     2521 CCTTCAAATA CCGGTTCAAG AAAGATGGAC GGAAGCACCA CTTGATCATC AACGAAGCGA 
     2581 CCCTGGAGGA CGCAGGACAC TATGCAGTTC GCACAAGTGG GGGCCAAGCA CTGGCTGAGC 
     2641 TCATTGTGCA AGAGAAGAAG CTGGAGGTCT ACCAAAGCAT CGCGGACCTG GCGGTGGGAG 
     2701 CGAAGGACCA GGCTGTGTTT AAGTGTGAGG TTTCAGATGA GAACGTGCGT GGCGTGTGGC 
     2761 TGAAGAACGG GAAGGAACTG GTGCCGGACA ACCGCATAAA GGTGTCCCAT ATAGGCCGGG 
     2821 TCCACAAACT GACCATTGAC GATGTCACAC CTGCCGATGA GGCTGACTAC AGTTTTGTGC 
     2881 CTGAAGGGTT TGCCTGCAAC CTGTCTGCCA AGCTCCACTT CATGGAGGTC AAGATTGACT 
     2941 TTGTGCCTAG GCAGGAACCT CCCAAGATCC ACTTGGACTG TCCTGGCAGC ACACCAGACA 
     3001 CCATTGTGGT TGTTGCTGGG AACAAGTTAC GCCTGGATGT CCCTATTTCT GGGGACCCTG 
     3061 CTCCCACTGT AATCTGGCAA AAGACTATAA CACAGGGGAA GAAGGCCTCA GCTGGGCCAC 
     3121 CCCCTGGTGC CCCAGAAGAT GCAGGTGCTG ATGAGGAGTG GGTGTTTGAT AAGAAGCTGT 
     3181 TGTGTGAGAC GGAGGGCCGT GTCCGTGTGG AGACCACCAA AGACCGAAGC GTCTTCACGG 
     3241 TCGAAGGGGC GGAGAAGGAA GATGAGGGTG TCTACACTGT CACAGTAAAG AACCCCGTGG 
     3301 GCGAGGACCA GGTCAACCTC ACAGTCAAGG TCATTGATGT GCCAGATGCT CCTGCAGCCC 
     3361 CTAAGATCAG CAACGTGGGC GAGGACTCCT GCATTGTGCA GTGGGAACCA CCTGCCTATG 
     3421 ATGGTGGGCA GCCGGTCCTG GGATACATCC TGGAGCGCAA GAAGAAAAAG AGCTACAGGT 
     3481 GGATGAGGCT CAACTTCGAT CTGCTGCGGG AGCTGAGCCA CGAAGCCAGG CGCATGATTG 
     3541 AGGGTGTAGC CTATGAGATG AGAGTCTACG CGGTCAATGC TGTGGGAATG TCCAGGCCCA 
     3601 GCCCTGCCTC TCAGCCCTTC ATGCCTATTG GGCCCCCTGG TGAACCAACT CACCTGACTG 
     3661 TGGAGGATGT GTCGGACACC ACAGTCTCAC TCAAGTGGCG GCCCCCAGAG CGTGTGGGTG 
     3721 CAGGTGGCCT GGACGGATAC AGTGTGGAGT ATTGCCAGGA GGGCTGCTCC GAGTGGGTGA 
     3781 CTGCTCTGCA GGGGTTGACA GAGCGCACTT CGCTGCTGGT GAAGGACCTA CCCACTGGGG 
     3841 CACGGCTGCT GTTCCGAGTG CGGGCACACA ATGTGGCAGG TCCTGGGGGC CCTATCATCA 
     3901 CCAAGGAGCC TGTAACAGTG CAGGAGATAC TGCAGCGACC ACGGCTCCAA CTGCCCAGAC 
     3961 ACCTGCGCCA GACCATCCAG AAGAAAGTTG GGGAGCCTGT GAACCTCCTC ATCCCTTTCC 
     4021 AGGGCAAACC CCGGCCTCAA GTGACCTGGA CAAAAGAGGG GCAGCCACTG GCAGGCGAGG 
     4081 AGGTGAGCAT CCGCAACAGC CCCACAGACA CAATCTTGTT CATCCGAGCT GCCCACCGCA 
     4141 CTCACTCGGG CACCTACCAG GTGACAGTTC GCATTGAGAA CATGGAGGAC AAGGCAACGC 
     4201 TGGTCCTGCA GATCGTGGAC AAGCCAAGTC CTCCCCTGGA TATCCGGGTC GTTGAGACTT 
     4261 GGGGCTTCAG TGTGGCTCTG GAGTGGAAGC CACCCCAAGA TGATGGCAAT ACAGAGATCT 
     4321 GGGGTTATAC TGTACAGAAA GCCGACAAGA AGACCATGGA GTGGTTCACT GTTTTGGAAC 
     4381 ATTACCGTCA GACTCACTGT GTGGTATCAG AGCTTATCAT TGGCAATGGC TACTACTTCC 
     4441 GGGTCTTCAG CCATAACATG GTGGGCTCCA GTGACAGAGC TGCTGCCACC AAGGAGCCCA 
     4501 TCTTTATTCC GAGACCAGGC ATCACATATG AGCCACCCAA ATACAAGGCC CTGGACTTCT 
     4561 CTGAAGCCCC AAGCTTCACC CAGCCCTTGA CAAACCGCTC CATCATTGCA GGCTACAATG 
     4621 CCATCCTCTG CTGTGCTGTC CGGGGTAGTC CTAAGCCCAA GATTTCCTGG TTCAAGAATG 
     4681 GCTTGGACCT GGGAGAAGAT GCTCGTTTTC GCATGTTCTG CAAGCAGGGA GTATTGACCC 
     4741 TGGAGATCAG GAAGCCCTGT CCCTATGATG GGGGTGTCTA TGTCTGCAGA GCCACCAACT 
     4801 TGCAGGGTGA GGCACAGTGT GAGTGCCGCC TGGAGGTGCG AGTTCCTCAG ACGCGTACGC 
     4861 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4921 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4981 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5041 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5101 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5161 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5221 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5281 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5341 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5401 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5461 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5521 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5581 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5641 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5701 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5761 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5821 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5881 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5941 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6001 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6061 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6121 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6181 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6241 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6301 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6361 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6421 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6481 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6541 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6601 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6661 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6721 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6781 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6841 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6901 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6961 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7021 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7081 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7141 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7201 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7261 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7321 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7381 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7441 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7501 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7561 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7621 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7681 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7741 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7801 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7861 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7921 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7981 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8041 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8101 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8161 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8221 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8281 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8341 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8401 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8461 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8521 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8581 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8641 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8701 TTT
//