LOCUS       RR207973                7908 bp    DNA     circular  SYN 
DEFINITION  Ptk2b (Myc-DDK-tagged ORF) - Rat PTK2B protein tyrosine kinase 2 beta (Ptk2b), (10 ug)
ACCESSION   NM_017318
VERSION     NM_017318.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4065..4156 
                     /label="Myc-DDK"  
     misc_feature    4186..4771 
                     /label="PolyA signal"  
     misc_feature    4243..4262 
                     /label="Restriction Enzyme"  
     misc_feature    5047..5719 
                     /label="ColE1"  
     misc_feature    6149..6943 
                     /label="Kan/Neo"  
     misc_feature    6978..7280 
                     /label="SV40 ori"  
     misc_feature    7366..7843 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4047..4053 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4047 
                     /label="Ptk2b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCGGGGTG TCTGAGCCCC TGAGCCGTGT AAAAGTGGGC ACTTTGCGCC 
     1081 CGCCCGAGGG CCCCCCAGAG CCCATGGTAG TGGTACCAGT AGACGTGGAG AAGGAAGACG 
     1141 TGCGTATCCT CAAGGTCTGC TTCTACAGCA ACAGCTTCAA CCCAGGGAAG AACTTCAAGC 
     1201 TTGTCAAATG CACAGTGCAG ACAGAGATCC AGGAGATCAT CACCTCCATC CTCCTGAGTG 
     1261 GGCGAATAGG GCCCAACATC CAGCTGGCTG AATGCTATGG GCTGAGGCTG AAGCACATGA 
     1321 AGTCAGATGA GATCCACTGG CTGCACCCAC AGATGACTGT GGGCGAAGTG CAGGACAAGT 
     1381 ATGAATGTCT ACACGTGGAG GCTGAGTGGA GGTATGACCT TCAAATCCGC TATTTGCCAG 
     1441 AAGACTTCAT GGAGAGCCTG AAAGAAGACA GGACTACACT GCTGTACTTT TATCAACAGC 
     1501 TCCGGAATGA CTACATGCAA CGCTATGCCA GCAAGGTCAG TGAAGGCATG GCTCTGCAGC 
     1561 TGGGCTGTCT GGAGCTCAGG AGATTCTTCA AGGACATGCC CCACAATGCA CTTGACAAAA 
     1621 AGTCCAACTT TGAACTCCTG GAAAAAGAAG TCGGTCTGGA CCTGTTTTTC CCAAAGCAAA 
     1681 TGCAGGAAAA CTTAAAGCCC AAGCAGTTCC GGAAGATGAT CCAGCAGACC TTCCAGCAGT 
     1741 ATGCATCACT CCGGGAGGAA GAGTGTGTCA TGAAATTCTT CAATACTCTG GCAGGCTTTG 
     1801 CCAACATCGA CCAGGAGACC TATCGCTGTG AACTCATTCA AGGGTGGAAC ATTACTGTGG 
     1861 ACCTGGTCAT TGGCCCTAAA GGCATCCGTC AGCTGACAAG TCAAGACACA AAGCCCACCT 
     1921 GCCTCGCCGA GTTCAAGCAG ATCAGATCCA TCAGGTGCCT CCCCTTGGAA GAGACCCAGG 
     1981 CAGTACTGCA GCTGGGCATC GAGGGTGCCC CCCAGTCCTT GTCCATCAAA ACCTCGTCCC 
     2041 TGGCAGAGGC TGAGAACATG GCTGACCTCA TAGATGGCTA CTGCAGGCTT CAAGGAGAGC 
     2101 ATAAAGGCTC TCTCATCATT CATGCCAAGA AAGATGGTGA GAAGAGGAAC AGCCTGCCTC 
     2161 AGATCCCCAC ACTAAACCTG GAGTCTCGGC GGTCCCACCT CTCAGAAAGC TGTAGCATAG 
     2221 AGTCAGACAT CTATGCAGAG ATTCCTGATG AGACCCTGCG AAGGCCAGGA GGCCCACAGT 
     2281 ATGGTGTTGC CCGTGAAGAT GTAGTTCTTA ACCGCATTCT GGGTGAAGGC TTCTTCGGGG 
     2341 AGGTCTACGA AGGTGTCTAC ACGAACCATA AAGGGGAAAA AATTAATGTG GCCGTCAAGA 
     2401 CCTGTAAGAA AGATTGTACC CTGGACAACA AGGAGAAGTT CATGAGTGAG GCAGTGATCA 
     2461 TGAAGAATCT TGACCACCCT CACATCGTCA AGCTGATTGG CATCATTGAA GAGGAACCCA 
     2521 CATGGATCGT CATGGAACTG TATCCTTATG GGGAGCTGGG ACACTACCTG GAGCGAAATA 
     2581 AAAATTCCCT GAAGGTACCC ACTCTGGTCC TGTACGCTCT GCAGATATGC AAAGCCATGG 
     2641 CCTATCTGGA GAGCATCAAC TGTGTCCACA GGGATATTGC TGTCCGGAAC ATCCTGGTGG 
     2701 CCTCTCCTGA GTGTGTGAAG CTCGGGGACT TTGGGCTCTC CCGGTACATT GAGGATGAGG 
     2761 ACTATTACAA AGCTTCCGTG ACCCGTCTCC CCATCAAATG GATGTCCCCC GAGTCCATCA 
     2821 ACTTTCGCCG CTTTACAACC GCCAGTGACG TCTGGATGTT TGCTGTATGC ATGTGGGAGA 
     2881 TCCTCAGCTT TGGGAAGCAG CCTTTCTTCT GGCTTGAAAA CAAGGATGTC ATCGGGGTGC 
     2941 TGGAGAAAGG GGACAGGCTG CCTAAGCCCG AACTCTGCCC GCCTGTCCTT TACACACTCA 
     3001 TGACCCGCTG CTGGGACTAT GACCCCAGCG ACCGGCCCCG CTTTACGGAG CTTGTGTGCA 
     3061 GTCTCAGTGA CATTTATCAG ATGGAGAGGG ACATTGCAAT AGAACAAGAA AGGAACGCTC 
     3121 GTTACCGACC CCCCAAAATA TTGGAGCCCA CTGCTTTTCA GGAACCCCCA CCCAAGCCCA 
     3181 GCCGGCCCAA GTACAAACAC CCTCCACAGA CCAATCTGCT GGCTCCTAAG CTGCAGTTCC 
     3241 AGGTCCCTGA GGGTCTGTGT GCCAGCTCTC CTACACTTAC GAGCCCTATG GAGTATCCAT 
     3301 CTCCAGTTAA CTCGCTGCAC ACCCCGCCTC TGCACCGACA CAATGTCTTC AAGCGCCACA 
     3361 GCATGCGGGA GGAGGACTTC ATCCGGCCCA GTAGTCGAGA AGAGGCCCAG CAGCTCTGGG 
     3421 AGGCAGAGAA GATCAAGATG AGGCAGGTCC TAGACAGACA GCAGAAGCAA ATGGTGGAAG 
     3481 ATTCCCAGTG GCTGAGGCGG GAGGAAAGAT GCTTGGACCC GATGGTTTAT ATGAATGACA 
     3541 AGTCCCCACT GACCCCAGAG AAGGAGGCCG GCTACACGGA GTTCACAGGG CCCCCCCAGA 
     3601 AGCCACCACG GCTCGGTGCA CAGTCCATCC AGCCTACAGC CAACTTGGAC AGGACTGATG 
     3661 ACCTCGTGTA CCACAATGTC ATGACCCTGG TGGAGGCCGT GCTGGAACTC AAGAACAAGC 
     3721 TCAGCCAGCT GCCTCCTGAG GAGTATGTGG TGGTGGTGAA GAATGTGGGG CTGAACCTGC 
     3781 GGAAGCTCAT CGGTAGCGTG GACGATCTCC TACCCTCCTT GCCAGCATCT TCTAGAACAG 
     3841 AGATTGAAGG GACCCAGAAA CTGCTCAACA AAGACCTGGC AGAGCTCATC AACAAGATGA 
     3901 GGTTGGCTCA GCAGAACGCT GTGACATCCC TAAGTGAGGA CTGCAAGCGG CAGATGCTCA 
     3961 CAGCATCCCA TACCCTGGCT GTGGATGCCA AGAACCTCCT GGATGCTGTA GACCAAGCCA 
     4021 AGGTTGTGGC TAATCTGGCC CACCCGCCTG CAGAGACGCG TACGCGGCCG CTCGAGCAGA 
     4081 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4141 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4201 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4261 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4321 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4381 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4441 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4501 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4561 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4621 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4681 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4741 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4801 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4861 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4921 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4981 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5041 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5101 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5161 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5221 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5281 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5341 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5401 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5461 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5521 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5581 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5641 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5701 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5761 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5821 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5881 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5941 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6001 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6061 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6121 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6181 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6241 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6301 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6361 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6421 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6481 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6541 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6601 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6661 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6721 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6781 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6841 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6901 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6961 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7021 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7081 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7141 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7201 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7261 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7321 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7381 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7441 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7501 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7561 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7621 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7681 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7741 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7801 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7861 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//