LOCUS       RR207971                8553 bp    DNA     circular  SYN 
DEFINITION  Copa (Myc-DDK-tagged ORF) - Rat coatomer protein complex subunit alpha (Copa), (10 ug)
ACCESSION   NM_001134540
VERSION     NM_001134540.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4710..4801 
                     /label="Myc-DDK"  
     misc_feature    4831..5416 
                     /label="PolyA signal"  
     misc_feature    4888..4907 
                     /label="Restriction Enzyme"  
     misc_feature    5692..6364 
                     /label="ColE1"  
     misc_feature    6794..7588 
                     /label="Kan/Neo"  
     misc_feature    7623..7925 
                     /label="SV40 ori"  
     misc_feature    8011..8488 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4692..4698 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4692 
                     /label="Copa"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTAACCAAA TTCGAGACCA AAAGCGCGCG GGTGAAAGGC CTTAGTTTTC 
     1081 ATCCTAAAAG ACCCTGGATC CTGACCAGTT TACATAATGG GGTCATCCAG TTATGGGACT 
     1141 ATCGGATGTG CACCCTCATT GACAAATTTG ATGAACATGA TGGTCCAGTG CGTGGCATTG 
     1201 ACTTCCATAA GCAGCAGCCC CTATTCGTCT CTGGAGGAGA TGACTATAAG ATTAAGGTTT 
     1261 GGAATTACAA ACTTCGGCGC TGCCTCTTCA CATTGCTTGG GCACTTGGAT TATATCCGCA 
     1321 CGACGTTTTT TCATCATGAG TATCCTTGGA TTCTGAGCGC CTCAGATGAT CAGACCATTC 
     1381 GGGTGTGGAA CTGGCAGTCT AGAACCTGTG TCTGTGTGTT AACGGGGCAC AACCATTATG 
     1441 TAATGTGTGC TCAGTTCCAC CCCTCTGAAG ACCTGGTGGT ATCAGCTAGC CTGGACCAGA 
     1501 CTGTCCGCGT TTGGGATATT TCTGGTCTAA GGAAAAAAAA CTTGTCTCCT GGTGCGGTGG 
     1561 AGTCCGATGT GAGAGGAATA ACTGGGGTCG ATCTGTTTGG AACTACAGAT GCAGTGGTGA 
     1621 AGCATGTGCT AGAGGGCCAT GACCGTGGAG TGAATTGGGC TGCCTTCCAC CCGACTATGC 
     1681 CCCTTATTGT ATCGGGGGCA GATGACCGGC AAGTGAAGAT ATGGCGCATG AATGAATCAA 
     1741 AGGCATGGGA GGTTGACACC TGCCGGGGCC ATTACAACAA TGTGTCTTGT GCCGTCTTTC 
     1801 ACCCTCGCCA AGAGTTGATC CTCAGCAATT CTGAGGACAA GAGTATTCGA GTCTGGGATA 
     1861 TGTCTAAGCG GACTGGGGTT CAGACTTTCC GTAGGGATCA TGATCGCTTC TGGGTCCTGG 
     1921 CTGCCCATCC CAACCTTAAC CTCTTTGCAG CAGGCCATGA TGGTGGGATG ATTGTATTTA 
     1981 AGCTGGAACG GGAGCGTCCA GCCTATGCTG TTCATGGCAA TATGCTGCAT TATGTGAAGG 
     2041 ACCGATTCCT CCGTCAGCTG GATTTCAACA GCTCCAAAGA TGTAGCTGTG ATGCAGTTGC 
     2101 GGAGTGGTTC TAAGTTCCCA GTGTTCAACA TGTCATACAA TCCAGCAGAG AACGCCGTGC 
     2161 TGCTGTGCAC CAGAGCAAGC AATTTAGAGA ATAGTACCTA TGATCTGTAC ACCATCCCCA 
     2221 AAGATGCAGA CTCCCAGAAT CCTGATGCCC CTGAAGGGAA ACGATCCTCG GGCCTGACAG 
     2281 CGGTTTGGGT TGCTCGGAAT CGGTTTGCTG TCCTAGATCG AATGCACTCG CTTCTGATTA 
     2341 AAAACCTGAA AAATGAGATC ACCAAGAAAA TCCAGGTGCC CAACTGTGAT GAAATCTTCT 
     2401 ATGCTGGTAC AGGCAACCTC CTGCTTCGGG ATGCAGATTC CATCACACTC TTTGATGTCC 
     2461 AGCAGAAGCG AACTCTGGCA TCAGTGAAGA TTTCCAAGGT GAAATATGTT ATCTGGTCGG 
     2521 CAGACATGTC TCATGTTGCA CTCCTGGCCA AGCATGCCAT TGTGATCTGT AACCGCAAGC 
     2581 TGGATGCTCT GTGTAACATT CATGAGAACA TTCGTGTCAA GAGTGGGGCC TGGGATGAAA 
     2641 GTGGGGTGTT TATCTATACC ACAAGCAACC ACATCAAATA TGCTGTCACC ACTGGGGACC 
     2701 ATGGGATCAT CCGAACTCTG GATTTGCCCA TCTATGTCAC AAGAGTGAAG GGCAATAACG 
     2761 TATACTGCCT GGACAGGGAG TGTCGTCCTC GGGTGCTCAC CATTGACCCC ACCGAATTCA 
     2821 AGTTCAAACT GGCCCTGATC AACAGGAAGT ATGATGAGGT ACTGCACATG GTGAGGAACG 
     2881 CCAAACTAGT AGGCCAGTCG ATCATCGCTT ACCTCCAGAA GAAGGGTTAT CCTGAAGTGG 
     2941 CACTGCATTT TGTAAAGGAT GAGAAAACTC GTTTCAGCTT GGCCCTGGAG TGTGGAAACA 
     3001 TTGAGATTGC TCTAGAGGCA GCCAAAGCTC TGGATGACAA GAACTGCTGG GAGAAGCTGG 
     3061 GAGAAGTGGC CCTGTTACAG GGCAATCACC AGATTGTAGA AATGTGCTAT CAGCGCACCA 
     3121 AAAACTTTGA CAAGCTCTCC TTCCTGTACC TTATTACTGG CAACCTGGAG AAGCTTCGAA 
     3181 AGATGATGAA GATTGCTGAG ATCCGAAAGG ACATGAGTGG CCATTATCAG AATGCCCTGT 
     3241 ACCTGGGTGA TGTGTCAGAG AGAGTGCGAA TCCTGAAGAA CTGTGGGCAG AAATCCCTGG 
     3301 CCTACCTCAC AGCTGCTACA CATGGCTTAG ATGAGGAAGC TGAGAGCCTG AAGGAGACAT 
     3361 TTGACCCTGA GAAGGAGACA ATCCCAGATA TTGATCCTAA TGCCAAGCTG CTCCAGCCAC 
     3421 CAGCACCTAT CATGCCATTG GATACCAATT GGCCCTTACT GACTGTGTCC AAAGGGTTCT 
     3481 TTGAAGGCTC CATTGCAAGC AAGGGGAAGG GAGGCGCGTT GGCTGCCGAC ATTGACATCG 
     3541 ACACTGTTGG TACTGAAGGC TGGGGAGAGG ACGCAGAGCT GCAGCTGGAC GAAGATGGGT 
     3601 TTGTGGAGGC TCCGGAAGGT TTGGGGGAGG ATACTCTCGG CAAGGGACAA GAGGAAGGAG 
     3661 GTGGCTGGGA CGTAGAAGAA GATTTGGAGC TCCCACCTGA GCTGGATGTA CCCTCTGGGG 
     3721 TTGCCGGTGG TGCTGAAGAC GGTTTCTTTG TGCCCCCAAC CAAGGGAACA AGCCCAACTC 
     3781 AAATCTGGTG CAATAACTCC CAGCTTCCAG TGGATCACAT CCTGGCAGGT TCTTTTGAAA 
     3841 CAGCTATGCG GCTTCTTCAT GACCAGGTGG GAGTAGTCCA GTTTGGCCCC TATAAGCAAC 
     3901 TGTTTCTACA GACATACGCG CGAGGCCGCA CCACCTACCA AGCACTGCCC TGCTTGCCCT 
     3961 CCATGTATAG CTACCCTAAT CGCAATTGGA AGGACGCAGG GCTGAAGAAT GGTGTGCCGG 
     4021 CTGTGGGCCT CAAGCTTAAC GACCTGATCC AGCGGCTGCA GCTCTGTTAC CAGCTCAGCA 
     4081 CAGTCGGCAA GTTTGAAGAC GCTGTGGAGA AATTCCGCTC CATCCTACTC AGTGTGCCCC 
     4141 TTCTTGTGGT GGACAATAAA CAGGAGATTG CAGAGGCCCA GCAGCTCATC ACCATCTGCC 
     4201 GTGAGTACAT TGTGGGTTTG TGCATGGAGA TAGAAAGGAA GAAGCTACCT AAGGAGACCC 
     4261 TAGACCAGCA GAAGCGCATC TGTGAGATGG CGGCTTATTT CACACACTCA AACCTGCAGC 
     4321 CTGTGCACAT GATCCTGGTG CTGAGGACAG CCCTCAACCT CTTCTTCAAG CTCAAGAACT 
     4381 TCAAGACAGC TGCCACCTTT GCCCGACGCC TGCTGGAACT TGGGCCCAAA CCTGAAGTAG 
     4441 CCCAACAGAC AAGGAAAATC TTGTCTGCCT GTGAGAAAAA CCCCACAGAT GCCTGCCAGC 
     4501 TCAATTATGA CATGCACAAC CCCTTTGACA TTTGTGCTGC ATCTTACCGG CCCATCTACC 
     4561 GTGGAAAACC AGTGGAGAAG TGTCCACTCA GTGGGGCCTG CTATTCCCCT GAGTTCAAAG 
     4621 GGCAGATCTG CAGGGTCACT ACAGTGACAG AGATTGGCAA AGATGTGATT GGTCTGAGGA 
     4681 TCAGTCCTCT TCAGTTTCGC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4741 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4801 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4861 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4921 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4981 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5041 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5101 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5161 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5221 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5281 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5341 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5401 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5461 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5521 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5581 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5641 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5701 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5761 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5821 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5881 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5941 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6001 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6061 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6121 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6181 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6241 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6301 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6361 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6421 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6481 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6541 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6601 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6661 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6721 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6781 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6841 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6901 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6961 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7021 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7081 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7141 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7201 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7261 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7321 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7381 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7441 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7501 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7561 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7621 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7681 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7741 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7801 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7921 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7981 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8041 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8101 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8161 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8221 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8281 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8341 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8401 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8461 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8521 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//