LOCUS       RR207955                7716 bp    DNA     circular  SYN 
DEFINITION  Enpep (Myc-DDK-tagged ORF) - Rat glutamyl aminopeptidase (Enpep), (10 ug)
ACCESSION   NM_022251
VERSION     NM_022251.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3873..3964 
                     /label="Myc-DDK"  
     misc_feature    3994..4579 
                     /label="PolyA signal"  
     misc_feature    4051..4070 
                     /label="Restriction Enzyme"  
     misc_feature    4855..5527 
                     /label="ColE1"  
     misc_feature    5957..6751 
                     /label="Kan/Neo"  
     misc_feature    6786..7088 
                     /label="SV40 ori"  
     misc_feature    7174..7651 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3855..3861 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3855 
                     /label="Enpep"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTTTGCA GAGGAAGAGC CCTCTAAGAA ATACTGCATT AAAGGCAAAC 
     1081 ACGTGGCCAT CATCTGCGCA ACGGTGGTAG CAGTGGGATT AATAGTAGGA CTTTCTGTGG 
     1141 GCTTGACCAG GTCGTGTGAG CCGGGCACAA CCCCCGCTCC TTCCAACCCT CCTCCCCACA 
     1201 CCAGCACTGC CCTGCCTCCC CAGGATCAGA ATGTCTGCCC TGACAGTGAC GATGAAAGTG 
     1261 GAGAATGGAA AAACTTCAGG CTGCCGGACT TCATCCAGCC AGTTCACTAC GACCTGGAGG 
     1321 TGAAGGTCCT AATGGAGGAA GACAGGTACA CGGGCATAGT GAGCATCTCT GTCAACTTGA 
     1381 GCAAAGACAC TCGTGACCTA TGGCTGCACA TCAGGGAAAC CAGGATCACC AAGCTGCCGG 
     1441 AGCTAAGGAG GCCCTCCGGG GAGCAGGTAC CGATTCGACG GTGCTTTGAG TACAAGAAGC 
     1501 AGGAGTACGT GGTGATTCAG GCTGAAGAAG ACCTTGCGGC CACCAGCGGG GACAGTGTCT 
     1561 ATCGGCTGAC CATAGAGTTT GAAGGCTGGT TGAACGGCTC CCTTGTGGGT TTTTACAGAA 
     1621 CCACCTACAC GGAGGACGGG CAAACCAAGA GCATAGCGGC CACTGACCAC GAACCAACAG 
     1681 ATGCCAGGAA GTCCTTCCCT TGTTTCGATG AACCCAACAA GAAGGCGACT TACAACATAT 
     1741 CCCTCATTCA CCCGAAAGAA TACTCAGCAC TTTCCAATAT GCCAGTAGAG AAGGAAGAGA 
     1801 CACTGGATAA CGACTGGAAG AAAACCACTT TCATGAAGTC CGTCCCGATG AGCACTTACC 
     1861 TGGTGTGCTT TGCTGTACAT CAGTTCACCT CGATACAGAG AACATCCAGG AGTGGCAAAC 
     1921 CACTCACTGT TTACGTCCAG CCCAACCAAA AGCAAACAGC AGAGTATGCG GCAAACATAA 
     1981 CCAAAGCTGT GTTTGACTTC TTTGAAGACT ACTTCGCTAT GGAGTATTCT CTTCCGAAAC 
     2041 TGGATAAAAT TGCTATTCCA GATTTTGGCA CCGGCGCCAT GGAAAACTGG GGACTTGTCA 
     2101 CTTACCGAGA AACAAACCTG CTTTATGACC CCCTGCTATC GGCCTCATCC AACCAGCAGA 
     2161 GAGTAGCCAG CGTGGTTGCC CACGAGCTCG TGCACCAGTG GTTTGGAAAT ATCGTGACCA 
     2221 TGGACTGGTG GGACGACTTG TGGCTAAATG AAGGATTTGC TTCGTTCTTC GAGTTCTTGG 
     2281 GAGTAAACCA CGCAGAAGCA GACTGGCAGA TGCTCAGTCA GGTGCTGCTT GAAGACGTGC 
     2341 TACCTGTGCA GGAGGATGAC TCCCTGATGT CCTCACATCC GGTGGTTGTC ACGGTGTCTA 
     2401 CTCCAGCTGA AATAACGTCT GTGTTCGATG GAATATCATA CAGCAAGGGA GCTTCTATCC 
     2461 TGAGAATGCT TCAAGACTGG ATAACACCAG AGAAATTCCA AAAGGGGTGT CAGATTTACT 
     2521 TGGAAAACTT CAAGTTCAAG AACGCGAAAA CTTCCGACTT TTGGGATTCA CTGGAAAAGG 
     2581 CAAGTAATCA ACCAGTGAAA GAAGTAATGG ACACCTGGAC TAGCCAGATG GGCTATCCTG 
     2641 TGGTTACTGT GAGTGGAAAG CAGAACGTCA CCCAGAAACG CTTTCTGTTG GACTACAAAG 
     2701 CTGACCCTTC CCAGCCACCG TCAGCACTTG GTTACACATG GAATATTCCA ATCAAATGGA 
     2761 CCGAAAATGG CAATTCAAAT ATCACAGTCT ACTATAGGTC AAACAGAGAA GGAATCACTT 
     2821 TGAATGCTAA TCTTAGCGGA GATGGTTTTC TTAAAATAAA CCCAGATCAC ATTGGGTTTT 
     2881 ATCGTGTAAA CTATGAAGCA GAAACTTGGG ATTGGATAGC TGAGACTCTC TCCTCAAACC 
     2941 ACATGAACTT CTCTTCTGCT GACCGGTCAA GTTTTATCGA TGATGCTTTT GCTCTGGCAA 
     3001 GAGCTCAACT TCTGGATTAT GAGAAGGCCC TGAACTTGAC CAGGTATCTC ACATCAGAAA 
     3061 AGGATTTCTT ACCATGGGAG AGAGTCATTT CAGCTGTAAG CTACATCATT AGCATGTTTG 
     3121 AAGATGACAG AGAGCTGTAC CCCCTGATAG AGACATACTT CCGAAGCCAA GTGAAGCCCA 
     3181 TTGCGGATTC TCTGGGATGG CAGGATACCG GAAGCCACAT CACAAAGTTA CTCCGGGCCT 
     3241 CTGTATTAGG ATTCGCATGC AAGATGGGAG CCGGAGAAGC CTTGGGCAAT GCTTCCCAGT 
     3301 TATTTGAGGC CTGGCTGAAA GGGAATGAAA GCATTCCTGT GAACCTCAGG CTGCTGGTGT 
     3361 ATCGCTATGG AATGCAGAAC TCTGGCAATG AGGCAGCGTG GAACTATACT CTAGAGCAGT 
     3421 ACCAGAAAAC ATCTCTAGCA CAAGAGAAAG AAAAGCTGCT CTATGGGTTA GCTTCGGTGA 
     3481 AGGATGTTAC ACTCTTGGCA AGGTATCTGG AAATGCTCAA AGACCCCAAT ATTATTAAAA 
     3541 CTCAGGACGT ATTTACAGTC ATCCGCTACA TCTCCTACAA CAGCTATGGG AAAAGCATGG 
     3601 CCTGGAACTG GATCCAACTC AACTGGGACT ATCTGGTCAA CAGATTTACA ATCAACGACA 
     3661 GATACCTTGG CCGGATCGTC ACCATAGCCG AGCCCTTCAA CACTGAGCTG CAGCTCTGGC 
     3721 AGATGCAGAG CTTCTTTGCA AAATATCCAA ATGCTGGCGC TGGAGCAAAA CCCAGAGAGC 
     3781 AAGTGCTGGA AACGGTGAAG AACAACATCG AGTGGCTAAA ACTGAACAGA AAGTCCATCA 
     3841 GCGAGTGGTT CACCAGCATG CCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3901 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3961 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4021 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4081 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4141 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4201 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4261 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4321 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4381 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4441 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4501 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4561 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4621 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4681 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4741 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4801 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4861 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4921 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4981 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5041 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5101 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5161 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5221 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5281 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5341 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5401 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5461 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5521 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5581 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5641 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5701 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5761 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5821 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5881 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5941 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6001 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6061 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6121 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6181 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6241 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6301 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6361 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6421 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6481 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6541 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6601 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6661 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6721 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6781 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6841 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6901 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6961 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7021 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7081 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7141 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7201 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7261 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7321 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7381 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7441 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7501 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7561 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7621 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7681 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//