LOCUS       RR207950                8190 bp    DNA     circular  SYN 
DEFINITION  Jak3 (Myc-DDK-tagged ORF) - Rat Janus kinase 3 (Jak3), (10 ug)
ACCESSION   NM_012855
VERSION     NM_012855.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4345..4436 
                     /label="Myc-DDK"  
     misc_feature    4466..5051 
                     /label="PolyA signal"  
     misc_feature    4523..4542 
                     /label="Restriction Enzyme"  
     misc_feature    5327..5999 
                     /label="ColE1"  
     misc_feature    6429..7223 
                     /label="Kan/Neo"  
     misc_feature    7258..7560 
                     /label="SV40 ori"  
     misc_feature    7646..8123 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4320..4327 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4320 
                     /label="Jak3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACCTCCA AGCGAGGAGA CACCTCTCAT CTCTCAGCGC TCTTGTAGCC 
     1081 TCTCATCCTC AGAAGCAGGA GCCCTGCATG TGCTCCTTCC TCCCCGGGGA CCTGGGCCTC 
     1141 CCCAGCGACT GTCATTCTCT TTTGGGGACT ACTTGGCTGA GGACCTGTGT GTGCGGGCTG 
     1201 CCAAGGCCTG TGGCATCCTG CCCGTTTATC ATTCGCTCTT CGCTCTGGCC ACCGAGGACT 
     1261 TGTCTTGCTG GTTCCCCCCA AGCCACATCT TCTCCATAGA GGACGTGGAC ACTCAAGTCT 
     1321 TGGTCTACAG GCTACGCTTT TATTTTCCTG GTTGGTTTGG ACTGGAGACC TGTCACCGCT 
     1381 TTGGGTTACA CAAAGATTTG ACCAGTGCCA TCCTTGACGT ACATGTTTTA GAACACCTCT 
     1441 TTGCCCAGCA CCGCAGTGAC CTGGTGAGTG GGCGTCTCCC AGTAGGCCTT AGCCTGAAGG 
     1501 ACCAGGGAGA GTTCCTGAGC CTAGCTGTGC TGGACCTGGC CCAGATGGCT CGTAAGCAGG 
     1561 CCCAGCGGCC AGGAGAGCTG CTGAAGTCCG TCAGTTACAA AGCCTGTCTG CCGCCCAGCC 
     1621 TGCGCGACCT GATCCAGGGC CAGAGCTTCG TGACGCGCAG GCGCATCCGC AGGACCGTGG 
     1681 TCCAGGCGCT GCGCCGTGTA GTAGCCTGCC AAGCCGACCG CTACGCACTC ATGGCCAAGT 
     1741 ACATCCTAGA CCTTGAGCGG CTGCATCCAG CGGCCACCAC TGAGTCCTTC CTCGTGGGAC 
     1801 TCCCGGGCGC CCAGGAGGAG CCGGGTTGCC TGCGTGTGAC AGGGGATAAT GGCATCGCCT 
     1861 GGAGCTCCAA GGACCAGGAG CTTTTCCAGA CCTTCTGTGA CTTTCCAGAA ATCGTGGATG 
     1921 TCAGCATCAA GCAGGCTCCA CGCGTGGGTC CGGCAGGGGA GCACCGGCTA GTCACCATCA 
     1981 CCAGGATGGA CGGTCACATC CTGGAAGCGG AGTTCCCGGG GCTGCCTGAG GCGCTGTCTT 
     2041 TCGTGGCGCT CGTGGATGGG TACTTTCGCC TGATCTGCGA CTCCAGGCAT TTCTTCTGCA 
     2101 AGGAGGTGGC ACCGCCACGG CTGCTGGAGG AAGAGGCGGA GCTGTGCCAT GGACCCATCA 
     2161 CGTTAGACTT TGCCATCCAC AAGCTGAAGG CTGCTGGCTC CCTCCCGGGC TCCTACATTC 
     2221 TCCGACGCAG CCCGCAGGAC TATGACAGCT TTCTTCTTAC TGCCTGTGTC CAGACTCCTC 
     2281 TTGGCCCCGA CTACAAGGGC TGCCTCATCC GCCAAGACCC CAGTGGGGCT TTCTCCCTGG 
     2341 TTGGTCTCAG CCAGCTGCAC AGAAGCCTAC AGGAGCTGCT CACAGCCTGC TGGCATTCTG 
     2401 GACTGCAAGT AGACGGCACT GCCCTGAACC TCACATCTTG CTGCGTCCCC AGACCCAAGG 
     2461 AAAAGTCCAA TTTGATCGTG GTGCGTAGGG GCCGCAACCC CACGCCTGCC CCCGGGCACT 
     2521 CCCCATCCTG CTGTGCGCTG ACTAAGTTGA GCTTCCACAC AATCCCAGCA GACAGCCTGG 
     2581 AGTGGCACGA GAATCTTGGT CACGGCTCTT TTACCAAGAT CTTCCATGGC CATAGGCGGG 
     2641 AGGTTGTGGA TGGTGAGACA CATGACACAG AAGTCCTCCT GAAGGTTATG GACTCCAGAC 
     2701 ACCAGAACTG CATGGAGTCC TTTCTGGAAG CCGCAAGCTT GATGAGCCAA GTATCCTACC 
     2761 CGCACCTGGT GTTGCTGCAT GGAGTCTGCA TGGCTGGAGA CAGCATCATG GTGCAGGAAT 
     2821 TTGTGTACCT TGGAGCAATT GACACGTACC TGCGCAAGCG TGGTCACCTG GTGCCGGCCA 
     2881 GCTGGAAACT GCAGGTGACC AAGCAGCTGG CCTATGCCCT TAACTACTTG GAGGACAAAG 
     2941 GCCTCCCTCA TGGCAACGTC TCAGCGAGGA AGGTGCTGCT AGCTCGTGAG GGGGTTGATG 
     3001 GGAATCCACC TTTCATCAAG CTGAGTGATC CTGGTGTCAG CCCAACTGTT TTGAGCCTGG 
     3061 AAATGCTCAC TGACAGGATT CCCTGGGTGG CCCCGGAGTG TCTCCAGGAA GCTGGAACGC 
     3121 TCAACTTGGA GGCTGACAAG TGGGGCTTTG GCGCCACCAC GTGGGAGGTG TTCAGCGGGG 
     3181 CACCCATGCA CATTACCTCG CTGGAGCCCG CCAAGAAGCT GAAGTTCTAT GAGGACCGGG 
     3241 GACAGCTGCC GGCCCTCAAA TGGACAGAGC TGGAGGGGCT CATCGCACAG TGCATGGCGT 
     3301 ATGATCCTGG CCGGCGCCCC TCCTTCCGAG CCATCCTCAG AGACCTCAAC GGCCTCATTA 
     3361 CATCAGATTA CGAGCTCCTC TCAGACCCCA CACCTGGCAT CCCGAATCCC CGAGATGAGC 
     3421 TGTGTGGTGG TGCCCAGCTC TATGCCTGCC AGGACCCCGC CATCTTCGAG GAGAGACACC 
     3481 TTAAGTACAT CTCCCTGCTG GGCAAGGGGA ACTTCGGCAG CGTGGAGCTG TGCCGCTACG 
     3541 ACCCTCTGGG AGACAATACG GGACCCCTGG TAGCAGTGAA GCAGTTACAG CACAGCGGGC 
     3601 CAGAACAGCA GAGGGACTTC CAGCGAGAGA TTCAGATCCT CAAGGCTCTA CACTGTGACT 
     3661 TCATCGTCAA GTACCGGGGA GTCAGCTATG GGCCTGGTCG CCAGAGCCTG CGGTTAGTGA 
     3721 TGGAGTACCT GCCCAGCGGC TGCCTGCGGG ACTTCCTGCA GCGCCACCGC GCGCGCCTGC 
     3781 ACAACGACCG CCTACTGCTG TTCGCCTGGC AGATCTGCAA GGGCATGGAG TACCTGGGCG 
     3841 CACGCCGCTG CGTGCATCGT GACCTGGCTG CGCGCAACAT CCTGGTGGAG AGTGAGGCGC 
     3901 ATGTGAAGAT TGCTGACTTC GGCCTCGCTA AGCTGCTGCC TCTCGGAAAA GACTACTACG 
     3961 TGGTCCGCGA GCCGGGCCAA AGTCCCATCT TCTGGTACGC CCCTGAGTCC CTATCTGACA 
     4021 ACATCTTCTC CCGCCAATCT GACGTGTGGA GCTTCGGCGT GGTGCTGTAT GAGCTCTTCA 
     4081 CCTACAGCGA CAAGAGCTGC AGCCCATCCA CTGAGTTCCT GCGCATGATG GGGCCTGAGC 
     4141 GTGAGGGATC CCCGCTCTGC CACCTCTTGG AGCTGCTGGC AGAGGGCCGC CGTCTCCCAC 
     4201 CACCCTCCAC CTGCCCTACT GAGGTTCAAG AGCTCATGCA GCTGTGCTGG TCGCCCAACC 
     4261 CACAAGACCG GCCAGCATTT GACACCCTGA GCCCCCAGCT GGATGCGCTG TGGCGCGGAA 
     4321 GCCCCGGAAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4381 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4441 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4501 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4561 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4621 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4681 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4741 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4801 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4861 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4921 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4981 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5041 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5101 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5161 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5221 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5281 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5341 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5401 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5461 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5521 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5581 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5641 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5701 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5761 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5821 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5881 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5941 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6001 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6061 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6121 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6181 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6241 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6301 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6361 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6421 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6481 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6541 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6601 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6661 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6721 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6781 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6841 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6901 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6961 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7021 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7081 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7141 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7201 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7261 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7321 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7381 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7441 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7501 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7561 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7621 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7681 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7741 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7801 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7861 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7921 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7981 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8041 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8101 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8161 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//