LOCUS       RR207887                7455 bp    DNA     circular  SYN 
DEFINITION  Srgap2 (Myc-DDK-tagged ORF) - Rat SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), (10 ug)
ACCESSION   NM_001134958
VERSION     NM_001134958.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3612..3703 
                     /label="Myc-DDK"  
     misc_feature    3733..4318 
                     /label="PolyA signal"  
     misc_feature    3790..3809 
                     /label="Restriction Enzyme"  
     misc_feature    4594..5266 
                     /label="ColE1"  
     misc_feature    5696..6490 
                     /label="Kan/Neo"  
     misc_feature    6525..6827 
                     /label="SV40 ori"  
     misc_feature    6913..7390 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3594..3600 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3594 
                     /label="Srgap2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGTCTCCA GCCAAATTCA AAAAGGATAA GGAGATCATC GCAGAGTATG 
     1081 ATACTCAGGT CAAAGAGATC CGGGCTCAGC TCACAGAGCA GATGAAATGC CTAGATCAGC 
     1141 AGTGCGAGCT CCGGGTGCAG CTGTTGCAAG ACCTGCAGGA CTTCTTCCGC AAGAAGGCTG 
     1201 AGATTGAGAT GGACTACTCT CGCAACCTGG AGAAGCTAGC CGAGCGCTTT CTAGCCAAGA 
     1261 CACGAAGCAC TAAGGACCAG CAATTTAAGA AGGACCAGAA TGTTCTCTCT CCAGTCAACT 
     1321 GCTGGAACCT CCTCTTAAAC CAGGTGAAGC GGGAGAGCAG GGATCATACC ACCCTGAGTG 
     1381 ACATCTACCT GAATAATATA ATTCCTCGAT TTGTTCAAGT CAGCGAGGAC TCAGGAAGAC 
     1441 TCTTTAAAAA GAGTAAAGAA GTTGGCCAGC AACTCCAGGA TGACTTGATG AAGGTCCTGA 
     1501 ACGAGCTTTA TTCGGTCATG AAGACATATC ACATGTACAA TGCTGACAGC ATCAGTGCTC 
     1561 AGAGCAAACT AAAGGAAGCA GAGAAGCAGG AAGAGAAGCA AATTGGCAAA TCAGTAAAGC 
     1621 AAGAGGACCG GCAGACCCCT CGCTCCCCTG ACTCCACGGC CAACGTCCGC ATTGAGGAGA 
     1681 AACATGTCCG GAGGAGTTCA GTGAAGAAGA TCGAGAAGAT GAAGGAGAAG CGACAAGCCA 
     1741 AGTACACAGA GAACAAGCTG AAGGCCATTA AAGCCCGGAA TGAGTATTTA TTGGCTTTGG 
     1801 AGGCAACCAA CGCATCTGTC TTCAAGTACT ACATCCATGA CCTGTCTGAT ATTATTGATC 
     1861 AGTGTTGTGA CCTCGGCTAC CATGCTAGCC TGAACCGGGC TCTACGCACT TTTCTGTCTG 
     1921 CTGAATTAAA TCTGGAACAA TCAAAACATG AAGGTCTGGA TGCTATTGAA AATGCAGTGG 
     1981 AAAACCTAGA TGCCACCAGT GACAAGCAGC GGCTCATGGA GATGTACAAT AATGTTTTCT 
     2041 GCCCCCCTAT GAAGTTTGAA TTCCAGCCCC ACATGGGAGA TATGGCCTCT CAGCTCTGTG 
     2101 CCCAGCAGCC TGTCCAGAGT GAGCTGGTGC AAAGACGCCA ACAACTGCAG TCTCGCTTAT 
     2161 CCACTCTGAA GATCGAGAAT GAAGAGGTGA AAAAGACGAT GGAGGCCACC CTGCAGACCA 
     2221 TACAGGACAT CGTGACTGTT GAGGATTTTG ATGTATCTGA CTGCTTCCAG TATAGCAATT 
     2281 CCATGGAGTC TGTCAAATCA ACTGTCTCAG AAACGTTCAT GAGCAAGCCT AGCATTGCTA 
     2341 AGAGGAGAGC TAACCAGCAG GAGACGGAAC AGTTCTATTT CACGAAAATG AAGGAGTACT 
     2401 TGGAGGGTAG GAACCTCATC ACTAAATTAC AAGCCAAGCA TGACCTCCTG CAGAAGACCC 
     2461 TGGGAGAAAG TCAGCGGACA GACTGTAGTC TTGCCAGGCG AAGCTCAACC GTGAGGAAAC 
     2521 AGGACTCCAG CCAGGCAATT CCTCTGGTGG TAGAAAGCTG CATCCGGTTT ATTAGCAGAC 
     2581 ATGGCCTACG ACATGAGGGG GTTTTCCGGG TGTCTGGGTC ACAAGTAGAA GTGAACGACA 
     2641 TAAAGAACGC CTTTGAGAGA GGAGAGGACC CCCTGGCTGG GGACCAGAAT GACCATGACA 
     2701 TGGACTCCAT AGCTGGCGTC CTCAAGCTTT ACTTCCGGGG GCTGGAACAC CCGCTCTTCC 
     2761 CCAAAGACAT CTTCCATGAC TTGATTGCCT GTGTCACAAT GGACAACCTG CAAGAGAGAG 
     2821 CTGTGCATAT CCGGAAAGTC CTTCTGGTCC TGCCCAAGCC CACTCTGATT ATCATGAGAT 
     2881 ACCTCTTTGC CTTCCTCAAT CACTTATCAC AGTTCAGTGA AGAGAACATG ATGGACCCCT 
     2941 ACAACCTTGC CATCTGCTTC GGGCCCTCAC TGATGTCAGT GCCAGAGGGC CACGACCAGG 
     3001 TGTCCTGTCA AGCCCACGTG AATGAACTGA TCAAAACCAT CATCATCCAA CATGAGAACA 
     3061 TTTTCCCAAA TCCCAGGGAG CTGGAGGGTC CCATCTACAG CAGAGGAGGG AGCATGGAGG 
     3121 ATTACTGTGA CAGCACTCAT GGAGAGACTA TCTCTGCCGA AGACTCCACC CAGGACGTTA 
     3181 CAGCGGAACA CCACACAAGT GACGATGAGT GTGAGCCCAT TGAAGCCATT GCCAAGTTTG 
     3241 ACTACGTAGG CCGGACAGCC CGAGAACTGT CTTTCAAGAA GGGAGCATCC CTGCTGCTTT 
     3301 ACCAGCGAGC CTCTGACGAC TGGTGGGAAG GCCGGCACAA TGGTATAGAC GGACTCATCC 
     3361 CCCATCAGTA CATCGTAGTC CAAGACACCG AGGACGGTGT CGTGGAGAGG TCCAGCCCCA 
     3421 AGTCTGAGAT TGAGGTCATG TCTGAGCCAC CTGAAGAAAA GGTGACAGCC AGAACGGGGG 
     3481 CCAGCTGTCC CAGTGGGGGT CATGTAGCTG ATATTTATCT TGCAAACATC AACAAAGGGA 
     3541 TTCCTCGTTC GCCCGCCCAT GAGCTGCCGC CTCCCCAAGA GGCACTCAGG CTATTGGAGA 
     3601 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3661 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3721 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3781 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3841 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3901 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3961 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4021 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4081 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4141 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4201 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4261 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4321 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4381 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4441 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4501 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4561 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4621 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4681 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4741 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4801 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4861 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4921 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4981 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5041 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5101 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5161 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5221 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5281 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5341 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5401 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5461 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5521 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5581 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5641 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5701 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5761 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5821 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5881 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5941 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6001 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6061 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6121 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6181 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6241 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6301 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6361 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6421 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6481 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6541 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6601 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6661 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6721 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6781 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6841 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6901 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6961 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7021 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7081 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7141 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7201 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7261 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7321 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7381 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7441 TTTAACGCGA ATTTT
//