LOCUS       RR207754L3                9345 bp    DNA     circular  SYN 
DEFINITION  Spns2 (Myc-DDK-tagged ORF) - Rat spinster homolog 2 (Spns2), (10 ug)
ACCESSION   NM_001144991
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7636..7816 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8544..9203 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7864..8483 
                     /label="ColE1"  
     misc_feature    5676..5699 
                     /label="DDK"  
     misc_feature    6511..6531 
                     /label="LR50"  
     misc_feature    5628..5699 
                     /label="MYC"  
     misc_feature    5709..5774 
                     /label="P2A"  
     misc_feature    5775..6374 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6520..7111 
                     /label="WPRE"  
     misc_feature    3360..3367 
                     /label="Restriction Enzyme"  
     misc_feature    5610..5616 
                     /label="Restriction Enzyme"  
     misc_feature    3360..5610 
                     /label="Spns2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCGG CGCGCCAGAT CTCAAGCTTA ACTAGCTAGC 
     3361 GGACCGACAT GATGTGCCTG GAATGCGCCT CGGCGGCGGC GGGCGGCGCG GAGGAGGAGG 
     3421 AGGCGGACGC CGAACGGCGG CGCCGGCGCC GGGGGGCGCA GCCAGGGGCT GGAGGTAGCG 
     3481 CTTGCTGCGG GGCGCGGGGT GTTGGGGGCG CTGGAGTCGT GTCCGCGGAC GAAGAGGTGC 
     3541 AGACGTTGTC CGGCAGCGTG AGGAGGGTCC CGTCCGGACT CCCCAGCATC CCCAGTACCC 
     3601 CGGGCTGCGC AGCCGCTGCC AAGGGCCCCG GCGCGCCGCA GCCCAAACCC GCCAGCTTGG 
     3661 GCCGAGGGCG GGGGGCAGCC GCTGCCATCC TGAGCTTGGG CAACGTGCTC AACTACCTGG 
     3721 ACAGGTACAC TGTGGCAGGT GTTCTTCTGG ACATCCAGCA GCACTTTGGG GTCAAGGACC 
     3781 GGGGCGCTGG CTTGCTGCAG TCAGTGTTCA TCTGCAGCTT CATGGTGGCT GCCCCCATCT 
     3841 TTGGCTACCT GGGCGATCGC TTCAATAGAA AGGTGATCCT CAGCTGCGGC ATCTTCTTCT 
     3901 GGTCGGCCGT CACCTTCTCT AGTTCTTTCA TCCCTCAGCA GTACTTCTGG CTGCTGGTCC 
     3961 TGTCCCGGGG GCTAGTAGGT ATTGGTGAAG CTAGCTATTC CACCATAGCA CCCACCATCA 
     4021 TCGGCGACTT ATTTACCAAG AACACACGCA CACTGATGCT ATCAGTCTTC TATTTTGCCA 
     4081 TCCCTCTGGG CAGTGGCCTG GGCTACATCA CAGGTTCCAG CGTGAAGCAG GCAGCTGGAG 
     4141 ACTGGCATTG GGCCCTGCGG GTTTCCCCTG TCCTGGGCAT GATCACAGGA ACACTCATCC 
     4201 TCATCTTGGT CCCAGCCACT AAGAGAGGCC ATGCTGATCA ACTTGGGGGG CAGCTCAAAG 
     4261 CACGGACCTC CTGGCTCCGA GACATGAAGG CCCTGATCCG GAACCGCAGT TACGTCTTCT 
     4321 CCTCCCTGGC CACATCCGCT GTGTCCTTCG CCACAGGAGC CCTGGGCATG TGGATTCCCC 
     4381 TCTATCTGCA CCGTGCTCAA GTTGTACAAA AGACAGCAGA GACTTGCAAC AGCCCGCCCT 
     4441 GTGGAGCCAA AGACAGCCTC ATCTTTGGGG CCATTACCTG CTTTACTGGC TTTCTGGGCG 
     4501 TAGTCACAGG CGCGGGAGCC ACTCGCTGGT GCCGCTTGCG CACTCAGCGC GCTGACCCCT 
     4561 TGGTGTGCGC CGTGGGCATG CTGGGATCTG CCATCTTCAT CTGCCTCATC TTTGTGGCTG 
     4621 CCAAGACCAG CATCGTGGGC GCCTATATCT GCATCTTTGT TGGCGAGACA CTGCTGTTTT 
     4681 CTAACTGGGC CATCACTGCA GACATCCTCA TGTATGTGGT CATCCCCACT CGACGAGCCA 
     4741 CTGCTGTGGC CTTGCAGAGC TTCACCTCCC ATCTGCTGGG GGACGCTGGA AGCCCCTACC 
     4801 TCATTGGCTT TATCTCGGAC CTAATCCGCC AGAGCACCAA GGACTCCCCA CTCTGGGAGT 
     4861 TTCTGAGCCT GGGCTATGCC CTCATGCTGT GCCCTTTTGT TGTGGTCCTG GGTGGCATGT 
     4921 TCTTCCTTGC CACTGCTCTC TTCTTCCTCA GCGATCGTGC CAAGGCTGAG CAGCAGGCTG 
     4981 ACGAGGTCCC TGCCCAGCGC CTCCGGTCTA CCGGCCCCAG TGATTCGATG TGCCAGAGCC 
     5041 GTGCCTGGGC CCAGTCCCCG CTGATGTGCC ACCCTGACCC CCGTCCGTCT CTCCCTCAGG 
     5101 GTGAACCAGC TGGTGATGCC GCCTGCATCC GTGAAAATCT GAGGTGGTGC CGCTGGGACA 
     5161 GCAATGAACT GCAGTTTAGA TTGGGAGAGG TTCTCCAGCA TCCTGGACCT GCAGGACGGT 
     5221 CGCAAAGCTT CCTGTGTGAC TCACAGTTGG GTACACACCC TCTTTGGCCT AGGACTGCCA 
     5281 GGACTGAGTG GCCCTATCTT CAAGAAGCTG CATCCTCAGT GAACCTAGAA GGCTGTGTGT 
     5341 GCTGGAGTCA CTCAGTTGGA CACAACTCCA GCCCTGGTTG GGGGCTGAAG GGCCAAGATA 
     5401 GAAGCAGCCA CCCTGCAGTG GGTATTTGGG GAGATTCTGG TCCACCCTTA GCTCATGTGA 
     5461 TCTTGAGGGA GCCACTATCC TCCCTAGACA GTGGAACCAA GGAGCTGGAG CTTCCTCCAT 
     5521 TGTGTGCCAT GCATGGCACC CTCCACAACT TTGAAGAAAC AGGCCCGGGG CTGTACACAG 
     5581 AAGACACCGC TCAGGTCTCA GGACCTCAGT TCCGGTTCAC GCGTACGCGG CCGCTCGAGC 
     5641 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5701 ACGATAAGGT TTGGGTAGGA AGCGGAGCTA CTAACTTCAG CCTGCTGAAG CAGGCTGGAG 
     5761 ACGTGGAGGA GAACCCTGGA CCTATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC 
     5821 GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA 
     5881 CGCGCCACAC CGTCGATCCG GATCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT 
     5941 TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG 
     6001 TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC 
     6061 CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC 
     6121 TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG 
     6181 ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC 
     6241 GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC 
     6301 GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA 
     6361 TGACCCGCAA GCCCGGTGCC TGATGTACAC CCAAACGGCC GGCCGCGGTC TGTACAAGTA 
     6421 GGATTCGTCG AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATACATGTT 
     6481 TAAGGGTTCC GGTTCCACTA GGTACAATTC GATATCAAGC TTATCGATAA TCAACCTCTG 
     6541 GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA 
     6601 TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT 
     6661 TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC 
     6721 AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT 
     6781 GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG 
     6841 GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC 
     6901 AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC 
     6961 ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC 
     7021 CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT 
     7081 CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGTCG ACCTCGATCG 
     7141 AGACCTAGAA AAACATGGAG CAATCACAAG TAGCAATACA GCAGCTACCA ATGCTGATTG 
     7201 TGCCTGGCTA GAAGCACAAG AGGAGGAGGA GGTGGGTTTT CCAGTCACAC CTCAGGTACC 
     7261 TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG 
     7321 GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA 
     7381 CCACACACAA GGCTACTTCC CTGATTGGCA GAACTACACA CCAGGGCCAG GGATCAGATA 
     7441 TCCACTGACC TTTGGATGGT GCTACAAGCT AGTACCAGTT GAGCAAGAGA AGGTAGAAGA 
     7501 AGCCAATGAA GGAGAGAACA CCCGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA 
     7561 CCCGGAGAGA GAAGTATTAG AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACATGGC 
     7621 CCGAGAGCTG CATCCGGACT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA 
     7681 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT 
     7741 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT 
     7801 TTAGTCAGTG TGGAAAATCT CTAGCAGCAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     7861 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     7921 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     7981 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     8041 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     8101 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     8161 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     8221 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     8281 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     8341 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     8401 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     8461 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     8521 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GATTACGCCC CGCCCTGCCA CTCATCGCAG 
     8581 TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA 
     8641 ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGTG 
     8701 AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC 
     8761 ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC 
     8821 AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG 
     8881 TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA 
     8941 CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAACTCC 
     9001 GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA 
     9061 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA 
     9121 CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA 
     9181 ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATACTCTTCC TTTTTCAATA TTATTGAAGC 
     9241 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     9301 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGAC
//