LOCUS       RR207754                7171 bp    DNA     circular  SYN 
DEFINITION  Spns2 (Myc-DDK-tagged ORF) - Rat spinster homolog 2 (Spns2), (10 ug)
ACCESSION   NM_001144991
VERSION     NM_001144991.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3328..3419 
                     /label="Myc-DDK"  
     misc_feature    3449..4034 
                     /label="PolyA signal"  
     misc_feature    3506..3525 
                     /label="Restriction Enzyme"  
     misc_feature    4310..4982 
                     /label="ColE1"  
     misc_feature    5412..6206 
                     /label="Kan/Neo"  
     misc_feature    6241..6543 
                     /label="SV40 ori"  
     misc_feature    6629..7106 
                     /label="f1 ori"  
     misc_feature    1060..1067 
                     /label="Restriction Enzyme"  
     misc_feature    3310..3316 
                     /label="Restriction Enzyme"  
     misc_feature    1060..3310 
                     /label="Spns2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCAGAT CTCAAGCTTA ACTAGCTAGC GGACCGACAT GATGTGCCTG 
     1081 GAATGCGCCT CGGCGGCGGC GGGCGGCGCG GAGGAGGAGG AGGCGGACGC CGAACGGCGG 
     1141 CGCCGGCGCC GGGGGGCGCA GCCAGGGGCT GGAGGTAGCG CTTGCTGCGG GGCGCGGGGT 
     1201 GTTGGGGGCG CTGGAGTCGT GTCCGCGGAC GAAGAGGTGC AGACGTTGTC CGGCAGCGTG 
     1261 AGGAGGGTCC CGTCCGGACT CCCCAGCATC CCCAGTACCC CGGGCTGCGC AGCCGCTGCC 
     1321 AAGGGCCCCG GCGCGCCGCA GCCCAAACCC GCCAGCTTGG GCCGAGGGCG GGGGGCAGCC 
     1381 GCTGCCATCC TGAGCTTGGG CAACGTGCTC AACTACCTGG ACAGGTACAC TGTGGCAGGT 
     1441 GTTCTTCTGG ACATCCAGCA GCACTTTGGG GTCAAGGACC GGGGCGCTGG CTTGCTGCAG 
     1501 TCAGTGTTCA TCTGCAGCTT CATGGTGGCT GCCCCCATCT TTGGCTACCT GGGCGATCGC 
     1561 TTCAATAGAA AGGTGATCCT CAGCTGCGGC ATCTTCTTCT GGTCGGCCGT CACCTTCTCT 
     1621 AGTTCTTTCA TCCCTCAGCA GTACTTCTGG CTGCTGGTCC TGTCCCGGGG GCTAGTAGGT 
     1681 ATTGGTGAAG CTAGCTATTC CACCATAGCA CCCACCATCA TCGGCGACTT ATTTACCAAG 
     1741 AACACACGCA CACTGATGCT ATCAGTCTTC TATTTTGCCA TCCCTCTGGG CAGTGGCCTG 
     1801 GGCTACATCA CAGGTTCCAG CGTGAAGCAG GCAGCTGGAG ACTGGCATTG GGCCCTGCGG 
     1861 GTTTCCCCTG TCCTGGGCAT GATCACAGGA ACACTCATCC TCATCTTGGT CCCAGCCACT 
     1921 AAGAGAGGCC ATGCTGATCA ACTTGGGGGG CAGCTCAAAG CACGGACCTC CTGGCTCCGA 
     1981 GACATGAAGG CCCTGATCCG GAACCGCAGT TACGTCTTCT CCTCCCTGGC CACATCCGCT 
     2041 GTGTCCTTCG CCACAGGAGC CCTGGGCATG TGGATTCCCC TCTATCTGCA CCGTGCTCAA 
     2101 GTTGTACAAA AGACAGCAGA GACTTGCAAC AGCCCGCCCT GTGGAGCCAA AGACAGCCTC 
     2161 ATCTTTGGGG CCATTACCTG CTTTACTGGC TTTCTGGGCG TAGTCACAGG CGCGGGAGCC 
     2221 ACTCGCTGGT GCCGCTTGCG CACTCAGCGC GCTGACCCCT TGGTGTGCGC CGTGGGCATG 
     2281 CTGGGATCTG CCATCTTCAT CTGCCTCATC TTTGTGGCTG CCAAGACCAG CATCGTGGGC 
     2341 GCCTATATCT GCATCTTTGT TGGCGAGACA CTGCTGTTTT CTAACTGGGC CATCACTGCA 
     2401 GACATCCTCA TGTATGTGGT CATCCCCACT CGACGAGCCA CTGCTGTGGC CTTGCAGAGC 
     2461 TTCACCTCCC ATCTGCTGGG GGACGCTGGA AGCCCCTACC TCATTGGCTT TATCTCGGAC 
     2521 CTAATCCGCC AGAGCACCAA GGACTCCCCA CTCTGGGAGT TTCTGAGCCT GGGCTATGCC 
     2581 CTCATGCTGT GCCCTTTTGT TGTGGTCCTG GGTGGCATGT TCTTCCTTGC CACTGCTCTC 
     2641 TTCTTCCTCA GCGATCGTGC CAAGGCTGAG CAGCAGGCTG ACGAGGTCCC TGCCCAGCGC 
     2701 CTCCGGTCTA CCGGCCCCAG TGATTCGATG TGCCAGAGCC GTGCCTGGGC CCAGTCCCCG 
     2761 CTGATGTGCC ACCCTGACCC CCGTCCGTCT CTCCCTCAGG GTGAACCAGC TGGTGATGCC 
     2821 GCCTGCATCC GTGAAAATCT GAGGTGGTGC CGCTGGGACA GCAATGAACT GCAGTTTAGA 
     2881 TTGGGAGAGG TTCTCCAGCA TCCTGGACCT GCAGGACGGT CGCAAAGCTT CCTGTGTGAC 
     2941 TCACAGTTGG GTACACACCC TCTTTGGCCT AGGACTGCCA GGACTGAGTG GCCCTATCTT 
     3001 CAAGAAGCTG CATCCTCAGT GAACCTAGAA GGCTGTGTGT GCTGGAGTCA CTCAGTTGGA 
     3061 CACAACTCCA GCCCTGGTTG GGGGCTGAAG GGCCAAGATA GAAGCAGCCA CCCTGCAGTG 
     3121 GGTATTTGGG GAGATTCTGG TCCACCCTTA GCTCATGTGA TCTTGAGGGA GCCACTATCC 
     3181 TCCCTAGACA GTGGAACCAA GGAGCTGGAG CTTCCTCCAT TGTGTGCCAT GCATGGCACC 
     3241 CTCCACAACT TTGAAGAAAC AGGCCCGGGG CTGTACACAG AAGACACCGC TCAGGTCTCA 
     3301 GGACCTCAGT TCCGGTTCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG 
     3361 GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC 
     3421 GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC 
     3481 AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA 
     3541 ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG 
     3601 GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG 
     3661 GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT 
     3721 TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG 
     3781 GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT 
     3841 CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA 
     3901 GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA 
     3961 GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG 
     4021 CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG 
     4081 TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     4141 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     4201 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     4261 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     4321 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     4381 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     4441 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     4501 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     4561 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     4621 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     4681 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     4741 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     4801 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     4861 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     4921 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA 
     4981 TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG 
     5041 GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG 
     5101 TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC 
     5161 CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT 
     5221 CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC 
     5281 CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT 
     5341 AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA 
     5401 TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG 
     5461 CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC 
     5521 AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC 
     5581 CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA 
     5641 GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT 
     5701 GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC 
     5761 AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA 
     5821 TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC 
     5881 TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG 
     5941 CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT 
     6001 CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG 
     6061 GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC 
     6121 AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC 
     6181 CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC 
     6241 TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA 
     6301 GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG 
     6361 GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA 
     6421 CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG 
     6481 GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG 
     6541 CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC 
     6601 CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 
     6661 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 
     6721 TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT 
     6781 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT 
     6841 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT 
     6901 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG 
     6961 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA 
     7021 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC 
     7081 GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA 
     7141 GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//