LOCUS       RR207746                8286 bp    DNA     circular  SYN 
DEFINITION  Itga7 (Myc-DDK-tagged ORF) - Rat integrin alpha 7 (Itga7), (10 ug)
ACCESSION   NM_030842
VERSION     NM_030842.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4443..4534 
                     /label="Myc-DDK"  
     misc_feature    4564..5149 
                     /label="PolyA signal"  
     misc_feature    4621..4640 
                     /label="Restriction Enzyme"  
     misc_feature    5425..6097 
                     /label="ColE1"  
     misc_feature    6527..7321 
                     /label="Kan/Neo"  
     misc_feature    7356..7658 
                     /label="SV40 ori"  
     misc_feature    7744..8221 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4425..4431 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4425 
                     /label="Itga7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGGATT CCGAGGTGCG ATTTCCTCGG GCTCCCCGGA ATTTGCTACC 
     1081 TTCTTAGTTT CTTGCTTGCC GGACTGCTCT TACCACGGGC TAGCGCCTTC AATCTGGATG 
     1141 TGATGGGTGC CATACGCAAG GAGGGAGAAC CTGGCAGCTT ATTCGGCTTT TCGGTGGCCC 
     1201 TACACCGACA ATTACAGCCC CGACCCCAGA GCTGGCTGCT GGTGGGTGCC CCCCAGGCCC 
     1261 TGGCTCTTCC CGGACAGCAG GCAAATCGCA CGGGAGGCCT CTTTGCTTGT CCCCTGAGCC 
     1321 TAGAGGAGAC AGACTGCTAC AGAGTGGACA TCGACAGGGG AGCTAATGTG CAGAAGGAAA 
     1381 GCAAGGAGAA CCAGTGGCTG GGAGTCAGCG TGCGGAGCCA GGGAGCCGGG GGCAAGGTTG 
     1441 TTACCTGTGC ACACCGATAT GAGTCTCGAC AGAGAGTGGA CCAGGTTTTG GAGACTCGGG 
     1501 ATGTGATTGG TCGTTGCTTT GTGCTGAGCC AGGACCTGGC CATCCGTGAT GAGTTGGATG 
     1561 GTGGGGAGTG GAAGTTCTGT GAAGGGCGCC CCCAGGGCCA CGAACAATTT GGGTTCTGCC 
     1621 AGCAGGGCAC AGCTGCCACC TTCTCCCCCG ACAGCCACTA CCTCATTTTT GGGGCTCCAG 
     1681 GAACCTATAA CTGGAAGGGG TTGCTTTTTG TGACCAACAT TGATAGCTCA GACCCCGACC 
     1741 AGCTGGTGTA TAAAACTTTG GACCCCGCTG ACCGGCTCAC AGGACCAGCC GGAGACTTGA 
     1801 CCTTGAATAG CTATTTAGGT TTCTCCATTG ATTCTGGGAA GGGTCTCATG CGCTCAGAAG 
     1861 AGCTGAGTTT TGTGGCAGGG GCCCCACGTG CCAACCACAA AGGGGCTGTG GTCATTCTGC 
     1921 GCAAGGATAG TGCCAGCCGC CTGATTCCTG AGGTTGTGCT GTCTGGGGAG CGCCTGACCT 
     1981 CTGGCTTTGG CTACTCCCTG GCTGTGACTG ACCTCAACAG TGATGGCTGG GCAGACCTGA 
     2041 TTGTGGGTGC TCCCTACTTC TTTGAACGCC AAGAAGAACT TGGAGGTGCC GTGTATGTGT 
     2101 ACATGAACCA GGGTGGCCAT TGGGCAGATA TCTCTCCTCT CCGACTCTGT GGCTCCCCTG 
     2161 ACTCCATGTT TGGGATCAGT TTGGCTGTAC TGGGGGACCT CAATCAAGAT GGCTTTCCAG 
     2221 ACATTGCTGT AGGAGCTCCC TTTGACGGAG ATGGGAAAGT CTTTATCTAC CATGGGAGCA 
     2281 GCCTGGGGGT GGTCACCAAA CCTTCACAGG TGCTGGAGGG TGAGGCTGTG GGTATCAAGA 
     2341 GCTTTGGTTA CTCCCTGTCT GGTGGCCTGG ATGTGGACGG AAATCACTAC CCGGACCTGC 
     2401 TTGTGGGCTC CCTGGCTGAT ACTGCTGCTC TGTTCAGGGC CAGACCTGTT CTACATGTCT 
     2461 CCCAAGAAAT CTTCATTGAT CCAAGAGCCA TCGATCTGGA ACAGCCCAAC TGTGCCGACG 
     2521 GACGCTTGGT CTGCGTGCAC GTAAAGGTCT GTTTCAGTTA TGTTGCTGTG CCCAGCAGCT 
     2581 ACAGCCCTAT TGTGGTCCTA GATTATGTGT TAGATGGGGA CACAGACCGG AGGCTCCGGG 
     2641 GCCAGGCTCC ACGTGTGACT TTCCCGGGCC GAGGCCCGGA TGATCTCAAG CATCAGTCCT 
     2701 CGGGCACCGT GTCGTTGAAG CACCAACATG ACAGAGTCTG TGGAGACACT GTGTTCCAGC 
     2761 TGCAGGAAAA CGTCAAAGAC AAGCTACGGG CCATTGTGGT GACCCTGTCA TATGGTCTCC 
     2821 AAACCCCTCG ATTAAGGAGG CAAGCGCCTG ACCAGGGGCT CCCCCTTGTG GCTCCCATCC 
     2881 TCAACGCTCA CCAGCCCAGT ACACAGAGGA CTGAGATCCA CTTCCTGAAG CAAGGCTGTG 
     2941 GTGACGATAA GATCTGTCAG AGCAACCTCC AGCTTGCGCA GGCCCAGTTC TGTTCCCGGA 
     3001 TCAGCGACAC AGAGTTCCAG GCTCTGCCCA TGGATCTGGA TGGGACCGCC CTGTTTGCGC 
     3061 TGAGCGGGCA GCCATTCATA GGCCTGGAGC TGACAGTCAC CAACCTGCCC TCTGACCCAG 
     3121 CCCGGCCTCA GGCAGATGGG GATGATGCTC ATGAAGCCCA GCTCCTGGCC ACCCTCCCAG 
     3181 CCTCTCTTCG ATACTCGGGA GTCCGCACTC TGGACTCTGT GGAGAAGCCG CTCTGCCTGT 
     3241 CCAACGAGAA TGCCTCTCAT GTCGAGTGTG AGCTCGGGAA CCCTATGAAG AGAGGCACTC 
     3301 AGGTCACCTT CTACCTCATC CTCAGCACCT CTGGAATCAC CATTGAGACC ACAGAGCTGA 
     3361 AGGTGGAATT GCTCTTAGCC ACGATCAGTG AGCAGGACCT GCATCCGGTC TCCGTTCGGG 
     3421 CTCATGTCTT CATTGAATTG CCGCTGTCCA TTTCAGGGGT GGCCACTCCT CAGCAACTCT 
     3481 TCTTCTCTGG CAAGGTGAAG GGGGAGAGCG CCATGCGGTC TGAGAGGGAC GTGGGCAGCA 
     3541 AGGTCAAGTA CGAGGTCACG GTCTCCAATC AAGGCCAGTC ACTCAATACT CTGGGCTCTG 
     3601 CCTTCCTCAA CATCATGTGG CCCCATGAGA TCGCCAATGG GAAATGGCTG TTGTACCCCA 
     3661 TGCGGGTGGA GCTGGAGGGT GGACAGGGGC CTGAGAAGAA AGGGATCTGC TCCCCAAGAC 
     3721 CCAACATCCT CCACCTGGAT GTGGACAGCA GGGATAGGAG GCGGCGAGAG CTGGGGCAGC 
     3781 CGGAGCCTCA GGAGCCTCCA GAGAAGGTGG AACCTAGCAC ATCTTGGTGG CCAGTGTCCT 
     3841 CCGCTGAGAA GAGAAACGTG ACTCTGGACT GTGCCCAGGG CACGGCCAAG TGTGTGGTAT 
     3901 TCAGCTGCCC ACTCTACAGC TTTGACCGTG CAGCCGTGCT TCATGTCTGG GGCCGCCTCT 
     3961 GGAACAGCAC CTTTCTGGAG GAGTACATGT CCGTGAAGTC CCTGGAGGTG ATCGTCCGAG 
     4021 CCAACATCAC AGTGAAATCC TCCATTAAGA ACTTGCTGCT CAGAGATGCA TCCACAGTGA 
     4081 TCCCAGTGAT GGTATACTTG GACCCGGTGG CTGTGGTTGC AGAAGGAGTC CCCTGGTGGG 
     4141 TCATCCTCCT GGCGGTGCTG GCTGGGCTGC TAGTACTGGC CCTGCTGGTG CTGCTGCTGT 
     4201 GGAAGCTGGG ATTCTTCAAG CGGGCGAAGC ACCCTGAGGC CACTGTACCC CAGTACCACG 
     4261 CAGTGAAGAT CCTTCGGGAA GACCGGCAGC AGTTCAAGGA GGAGAAGACA GGCACCATTC 
     4321 AGAGGAGTAA CTGGGGCAAT TCCCAGTGGG AGGGCTCCGA CGCACACCCT ATCCTGGCTG 
     4381 CCGACTGGCA CCCTGAGTTG GGTCCTGATG GACATCCTGT GTCAGTCACT GCCACGCGTA 
     4441 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4501 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4561 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4621 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4681 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4741 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4801 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4861 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4921 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4981 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5041 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5101 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5161 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5221 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5281 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5341 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5401 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5461 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5521 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5581 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5641 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5701 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5761 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5821 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5881 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5941 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6001 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6061 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6121 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6181 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6241 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6301 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6361 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6421 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6481 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6541 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6601 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6661 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6721 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6781 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6841 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6901 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6961 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7021 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7081 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7141 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7201 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7261 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7321 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7381 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7441 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7501 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7621 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7681 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7741 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7801 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7861 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7921 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7981 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8041 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8101 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8161 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8221 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8281 AATTTT
//