LOCUS       RR207699                8529 bp    DNA     circular  SYN 
DEFINITION  Recql4 (Myc-DDK-tagged ORF) - Rat RecQ protein-like 4 (Recql4), (10 ug)
ACCESSION   NM_001130494
VERSION     NM_001130494.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4686..4777 
                     /label="Myc-DDK"  
     misc_feature    4807..5392 
                     /label="PolyA signal"  
     misc_feature    4864..4883 
                     /label="Restriction Enzyme"  
     misc_feature    5668..6340 
                     /label="ColE1"  
     misc_feature    6770..7564 
                     /label="Kan/Neo"  
     misc_feature    7599..7901 
                     /label="SV40 ori"  
     misc_feature    7987..8464 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4668..4674 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4668 
                     /label="Recql4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGGCTT GCGACCGTTC GCGCGTGGCT GCAGGATTGG GAACGCGCTT 
     1081 TTGTACGGCT GCACGGGCGA CGGCCAGCAA AGGAGGATGT GGAGGCGGCA CCCGAGGAGA 
     1141 CCCGCGCGCT CTACCGTGAG TACCGTAACC TAAAGCAGGC AGTGAGTCAG GCCGACGACG 
     1201 GACACCGCGT CCAAAAGCAA TCACTTGCCA AGGCAGCTGA GGAGGAACAG GAGCCAAGCT 
     1261 GCTGGGGTTC CCACCTGAAC CGAGCTGCAA CCCAGAACAC ACAGTCTATT CCAAAGCAGA 
     1321 GCCCACTGAG TTCTATACAA GACTATGGGA AGAGGCTCAA AGCCAATCTT AAAAACACAC 
     1381 TACAGGGCGG GCCAACCCTG AGTCGAAAAC TCCAGCATCA GAAGAGATCC TTGTCCATAG 
     1441 TGCCCACATC AAGGCCACCA GGCCCAAGGA CTGAATCCCC CTGTCCAGAA CAAGCTAATG 
     1501 ATGCTCTTCC CCAGGTTCCT GTGCCCCAGC CAAGGCAAGG CCAACTCCAG AAGCTCCAGT 
     1561 CATCCCTGAG CCTGAGGTTG AGTTCCCTAG ACCCTGGTTG GTTAGAGAGG TGTCAAAACA 
     1621 GAGTTTCAGA TCCTCTAGAG GTTCCAGGTG CTTGTGGGCT TGACCTGAGT GCAAAGGAGT 
     1681 CACAACCTCA GATGTCAGGC AAGGTGAATG TCATTAGTCC TGACATCCTT TCAGAAGTAT 
     1741 CTTCACAGAG CCCACAGGCC CTAGCCCAAC AGCCAGCCCA AGTTTTGTCA CAGATCCCCA 
     1801 AATCCAGCAA CAGTAAAGAC AGGAAGCGGA AACGGAATGA GAAGGGGGAG GACTTTGCAC 
     1861 AAGCCCAGCC CAGCAGCCAA GCAGGACCCC TGTCTGAGGG AGCTGGGGCT ACAGCACATG 
     1921 GGCAAGGCCC TCCAGGAGAA CCCACACAAG TGACTGTCCC TCAGCACCGC TGCTCTTCAA 
     1981 ACCAGGCCAG GACAGAGAAT GCTAAGAGTG CGGCCCACCT CCCCATGTCC CCTCGGCCAG 
     2041 CTTCCCTAGA CAGAGGGAAC TATGTTCGAC TTAACATGAA AACCAAATGC TTTGTACGAG 
     2101 TTGGGGCCAG TCGGGGCAGG CTTCTCCGAA AGCAGGTATG GAAGCAAAAG TGGAAGAAGA 
     2161 AACAAGAGTG GTTTGGGAAA AGTGGACCCA GGGCCACAGT CAAGGACACT TGTTTCCGGT 
     2221 GTGGGCAGTT TGGTCACTGG GCATCCCAGT GTTCCCAACC AGGCTCCACC CTGACCATCC 
     2281 AGCCAAAAGG TGACAGGAAT GACAAACATC CCATTTCCAC GTTGGGGGGA GTAGCACAGA 
     2341 AGACTGGCAC CACTTCCTGT AACCACTCTG GTGAGGAAAC TGAACCTGCT GGACCAGTGC 
     2401 TACCGGTGCC TCATTGCCCC ACGCCAGTGT TACCCCTCTA CCCACCAGGG CCCTTGGGAC 
     2461 AGGTAGCAGA CACCCCTGCT GAGGTATTCC AAGTCCTGGA GCAGCTAGGG CACCACACCT 
     2521 TCCGCCCTGG GCAAGAGCGT GCTGTCATGA GGATTCTTTC TGGCATCTCC ACACTGTTAG 
     2581 TGTTGCCCAC GGGTGCTGGC AAGTCTCTGT GCTACCAGCT TCCTGCACTG CTCTATGCCA 
     2641 AGCGAAGCCC CTGTCTCACA CTGGTCGTCT CTCCTCTCCT GTCACTCATG GATGACCAGG 
     2701 TGTCTGATCT GCCTTCATGT CTGAAGGCGG CCTGCCTCCA CTCAGGAATG ACCAAGAAAC 
     2761 AACGAGAATC TGTCTTGAAG AAGGTACGGG CAGCCCAGGT GCATGTGCTG ATTGTGTCCC 
     2821 CAGAGGCCTT GGTGGGATGC GGGGCTAAGG GTCCTGCCAA CCTCCCCCCG GCCGCTCAGC 
     2881 TGCCTCCAGT CGCCTTTGCC TGCATTGATG AGGTCCACTG CCTCTCTCAG TGGTCACATA 
     2941 ACTTCCGGCC CTGCTACTTA CGTGTTTGTA AAGTACTTCG GGAGCATATG GGTGTGCGCT 
     3001 GCTTTTTGGG TCTCACAGCC ACAGCCACAC GAAGCACTGC TCGTGATGTG GCCCAGCACC 
     3061 TGGGCATAGC TGAAGAGCTT GAGGTCAGTG GGTCAGCCAG CATCCCTGCC AATCTGCACC 
     3121 TCTCCGTGTC CATGGATCGA GACTCAGACC AGGCTCTGGT GACACTGCTA CAAGGTGACC 
     3181 GCTTCCGTAC CCTGGATTCG ATTATCATTT ACTGTGCTCG ACGAAAGGAC ACAGAGCGGG 
     3241 TGGCTGCCCT CCTCCGGACC TGCCTGTCCA CAGTGAGAGA TTCAAAGCCA AGAGGCCGTG 
     3301 GCCCTGAGAC TTTAGCGGAA GCATACCATG CTGGCATGTG TAGCCAGGAA CGGAAACGAG 
     3361 TACAACAGGC CTTCATGCAG GGCCACCTGC GCATGGTAGT GGCCACAGTG GCATTTGGGA 
     3421 TGGGACTGGA CCGTCCAGAT GTTCGGGCTG TGCTACACCT GGGACTGCCT CCAAGCTTCG 
     3481 AGAGCTACGT GCAAGCTATT GGCCGTGCAG GACGTGATGG GAAGCCTGCC CATTGCCACC 
     3541 TGTTCCTGCA TCCTCAGGGT GAAGACCTCT GGGAACTGAG CAGACATGTC CATGCTGATA 
     3601 GCACTGACTT CCTGGCTGTG AAGAAGCTGG TACAGCGTGT GTTCCCAGCC TGCTCATGCG 
     3661 CCCAGAGGCC TATGTCCCCA CTTGAGGAAG TCAAAGAGCA CAGTGGCCAA CAAACATACC 
     3721 CTGTACTGGG ACTAGCCTGC CGGGGCCATA AGCGGGCACT CCCAGTGCAG TCTACAGTAC 
     3781 TGGCTCTGGA CATGAAAGAG GAGGCCATTG AGACTTTGCT GTGCTATTTG GAACTACACC 
     3841 CTCGGCACTG GTTGGAGCTG CTGCCATACA CCTATGCCCA CTGCCATCTG CGTTGCCCTG 
     3901 GGGGCAGTGC CCAGCTGCAA GCTCTGGTCC ACAGGTGTCC CCCTTTGGCT GCATGCTGGG 
     3961 CCAAGTGGCC ACCTAAAGAC ACAAGTCAGG GTAGCAGCGC CTTAGAGTTT GATGTGGTGG 
     4021 AACTGGCAGA CTCCATGGGC TGGGAGTTGG CCTCTGTACG GCAGGCTCTC CACCAGCTGA 
     4081 AGTGGGACCC AGAGCCAAAG AAAGGTGCAC CACAGGGCAC AGGAGTGCTT GTGGAGTTCA 
     4141 GTGAGTTGGC CTTTCACCTG CACAGTCGTG GGGACCTGAC AGATGAGGAG AAGGACCAGA 
     4201 TCTGTGACTT TCTGTACAGC CGTGTGCAGG CTCGTGAACA CAAGGCCCTG GCCCACCTAC 
     4261 ACCAAATGTC CAAGGCCTTT CAAAGTGTAG CATTTCCCAG TTGTGGACCC TGTTTAGAGC 
     4321 AGTCTGACGA GGGGCACAGC AATCGGGTGA AGACCCTGGT CAGCTACTAC TTTGAGGAGG 
     4381 AGGAGGAGGA GGAAGAGGAA GAAACTATGA AGGATACTCA GGATCCAAAA CCTGGGCAGA 
     4441 CCCAGCTTCA GGATTGGGAG GACCAAGTTC GCCGCGATGT CCACCAGCTC CTGTCCCTGA 
     4501 GGCCAGAAGA AAAGTTTTCA GGAAGGGCTG TGGCCCGCAT CTTCCATGGA ATTGCAAGTC 
     4561 CATGCTACCC AGCCCAGGTG TTTGGGCAGG ACCGGCGCTT CTGGAGAAAG TACCTACACC 
     4621 TGGACTTCCA CGCCCTCATG CACCTAGCCA CAGAGGAGCT CCTGCTCAGA GGCCGAACGC 
     4681 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4741 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4801 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4861 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4921 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4981 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5041 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5101 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5161 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5221 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5281 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5341 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5401 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5461 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5521 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5581 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5641 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5701 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5761 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5821 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5881 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5941 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6001 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6061 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6121 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6181 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6241 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6301 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6361 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6421 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6481 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6541 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6601 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6661 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6721 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6781 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6841 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6901 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6961 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7021 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7081 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7141 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7201 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7261 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7321 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7381 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7441 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7501 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7561 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7621 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7681 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7741 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7981 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8041 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8101 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8161 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8221 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8281 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8341 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8401 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8461 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8521 GCGAATTTT
//