LOCUS       RR207698                8247 bp    DNA     circular  SYN 
DEFINITION  Stag1 (Myc-DDK-tagged ORF) - Rat stromal antigen 1 (Stag1), (10 ug)
ACCESSION   NM_001108179
VERSION     NM_001108179.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4404..4495 
                     /label="Myc-DDK"  
     misc_feature    4525..5110 
                     /label="PolyA signal"  
     misc_feature    4582..4601 
                     /label="Restriction Enzyme"  
     misc_feature    5386..6058 
                     /label="ColE1"  
     misc_feature    6488..7282 
                     /label="Kan/Neo"  
     misc_feature    7317..7619 
                     /label="SV40 ori"  
     misc_feature    7705..8182 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4386..4392 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4386 
                     /label="Stag1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTACTTCA GAGTTACCAG TGTTACAGGA TTCAACTAAT GAAACTACTG 
     1081 CCCATTCTGA TGCTGGCAGC GAACTTGAAG AAACAGAGGT CAAAGGAAAG AGAAAAAGGG 
     1141 GTCGTCCTGG CCGGCCTCCA TCAACAAATA AGAAACCTCG AAAATCTCCA GGAGAAAAGA 
     1201 GCAGAATTGA AGCTGGAATT AGAGGAGTGG GTCGCGGAAG AGCTAATGGG CACCCTCAAC 
     1261 AGAACGGCGA AGGGGATCCC GTCACCTTAT TTGAGGTGGT GAAGCTGGGG AAGAGTGCAA 
     1321 TGCAGTCTGT GGTGGATGAC TGGATTGAAT CATATAAACA AGACCGGGAC ATCGCACTTC 
     1381 TGGATTTAAT CAACTTTTTT ATCCAGTGTT CAGGATGTCG AGGTACTGTC AGAATAGAGA 
     1441 TGTTTCGAAA TATGCAGAAC GCAGAAATAA TCAGAAAAAT GACTGAAGAA TTTGATGAGG 
     1501 ACAGTGGTGA CTACCCCCTT ACCATGCCTG GTCCTCAGTG GAAAAAATTT CGTTCCAATT 
     1561 TTTGTGAATT TATTGGAGTC CTGATTCGAC AGTGTCAATA TAGCATAATT TATGATGAAT 
     1621 ATATGATGGA CACCGTAATT TCCCTTTTGA CTGGTTTGTC AGACTCCCAG GTCAGAGCTT 
     1681 TTAGGCATAC AAGTACCCTT GCTGCAATGA AGCTGATGAC TGCTCTGGTG AATGTTGCCT 
     1741 TAAACCTCAG TATTCATCAG GATAATACAC AGAGGCAATA CGAAGCCGAG AGAAATAAAA 
     1801 TGATTGGGAA GAGAGCCAAT GAAAGGCTGG AGTTACTACT TCAGAAACGT AAAGAGCTAC 
     1861 AAGAAAATCA AGATGAAATT GAAAATATGA TGAACTCTAT TTTTAAGGGT ATATTTGTTC 
     1921 ATAGATACCG TGATGCAATT GCTGAAATTC GAGCCATCTG TATTGAAGAA ATTGGAGTAT 
     1981 GGATGAAAAT GTATAGCGAT GCCTTCCTCA ATGACAGTTA CCTGAAATAT GTAGGATGGA 
     2041 CTCTTCATGA TAGGCAAGGT GAAGTCAGAC TGAAGTGTTT GAAAGCTCTA CAAAGCCTGT 
     2101 ATACCAATAG AGAATTATTC CCCAAATTGG AGCTGTTTAC TAATCGATTC AAGGATCGCA 
     2161 TTGTATCAAT GACTCTTGAC AAAGAATATG ATGTTGCTGT GGAAGCCATC CGGTTGGTTA 
     2221 CTCTGATACT TCATGGAAGT GAAGAAGCTC TTTCCAATGA AGACTGTGAA AACGTCTACC 
     2281 ATTTGGTGTA CTCAGCACAT CGCCCTGTTG CTGTGGCAGC TGGAGAGTTT CTGCACAAAA 
     2341 AGCTGTTTAG CAGACATGAC CCACAAGCTG AGGAAGCATT AGCAAAGAGA AGAGGAAGAA 
     2401 ACAGTCCAAA TGGGAACCTC ATTAGGATGC TTGTTCTTTT CTTTCTTGAA AGTGAGTTAC 
     2461 ATGAACATGC AGCCTACTTG GTGGACAGCT TGTGGGAGAG CTCTCAAGAA CTGTTGAAAG 
     2521 ACTGGGAATG TATGACAGAG TTACTATTAG AAGAACCTGT TCAAGGAGAG GAAGCCATGT 
     2581 CTGACCGTCA GGAGAGCGCT CTTATAGAGC TAATGGTCTG TACAATTCGT CAAGCAGCTG 
     2641 AGGCACATCC TCCAGTGGGA AGGGGAACCG GCAAGAGAGT GCTAACAGCC AAAGAAAGGA 
     2701 AAACTCAAAT TGATGATAGA AATAAATTAA CTGAACATTT TATCATTACA CTTCCTATGT 
     2761 TACTATCAAA GTATTCTGCA GATGCAGAGA AGGTAGCGAA CTTGCTGCAG ATTCCACAGT 
     2821 ATTTTGATCT GGAAATCTAC AGCACAGGTC GGATGGAAAA GCACCTGGAT GCTTTATTAA 
     2881 AACAGATTAA GTTTGTTGTA GAGAAACATG TAGAGTCAGA TGTCCTAGAA GCCTGCAGTA 
     2941 AAACCTACAG CATTTTATGC AGTGAAGAGT ACACCATTCA AAATCGAGTC GATATTGCTC 
     3001 GGAGCCAGCT CATTGATGAA TTTGTAGATC GATTCAATCA TTCTGTAGAA GACCTATTGC 
     3061 AAGAGGGTGA AGAAGCTGAT GATGATGATA TCTACAATGT TCTTTCCACC TTAAAGCGTT 
     3121 TAACTTCTTT TCACAATGCT CATGATCTCA CTAAATGGGA TCTGTTTGGT AATTGCTACA 
     3181 GACTATTGAA GACTGGAATT GAACATGGAG CTATGCCAGA ACAGATAGTC GTACAAGCAC 
     3241 TGCAGTGTTC CCATTACTCA ATTCTTTGGC AGCTGGTGAA AATTACTGAT GGTTCTCCTT 
     3301 CCAAATATTA CAATGACTAT GGTGATATTA TTAAGGAAAC CCTGAGTAAG ACCAGGCAGA 
     3361 TTGATAAGAT TCAGTGTGCT AAGACTCTCA TTCTCAGTCT ACAACAGTTG TTTAATGAAC 
     3421 TTGTTCAAGA ACAAGGTCCT AATCTGGATA GGACATCGGC CCATGTCAGT GGAATTAAAG 
     3481 AACTGGCACG TCGTTTTGCC CTTACATTTG GATTGGACCA GATCAAGACC CGAGAAGCAG 
     3541 TTGCCACACT TCACAAGGAT GGAATAGAGT TTGCCTTTAA ATACCAGAAT CAGAAAGGAC 
     3601 AAGAGTATCC ACCTCCTAAT CTGGCTTTTC TTGAAGTACT AAGTGAATTT TCTTCTAAAC 
     3661 TCCTTCGACA GGACAAGAAA ACTGTCCATT CCTATCTTGA AAAATTCCTC ACTGAGCAGA 
     3721 TGATGGAAAG GAGGGAGGAT GTATGGCTTC CACTCATCTC GTATAGAAAT TCGTTAGTCA 
     3781 CTGGGGGTGA AGACGACAGA ATGTCTGTGA ACAGTGGAAG TAGCAGCAGT AAAACTTCCT 
     3841 CAGTGCGCAG TAAGAAAGGA AGACCCCCAC TGCACAGAAA ACGAGCAGAA GATGAAAGTC 
     3901 TGGATAACAC ATGGCTAAAT AGGACTGACA CAATGATTCA GACTCCTGGA CCCTTGCCAA 
     3961 CCCCACAGCT CACCTCCACT GTACTTAGAG AGAACAGCCG TCCCATGGGA GAGCAGATTC 
     4021 AGGAGCCTGA GTCTGAGCAT GGTTCTGAAC CAGACTTTTT ACACAATCCC CAGATGCAGA 
     4081 TCTCTTGGTT AGGCCAGCCG AAGTTAGAAG ACTTGAATCG GAAGGACAGA ACAGGAATGA 
     4141 ACTACATGAA AGTGAGAGCT GGAGTAAGGC ATGCTGTTCG GGGTCTAATG GAGGAAGATG 
     4201 CTGAGCCCAT CTTTGAAGAT GTGATGATGT CATCACGGAG CCAGTTAGAA GACATGAATG 
     4261 AAGAATTTGA AGACACCATG GTTATTGATT TGCCCCCATC AAGAAATCGG CGAGAGCGAG 
     4321 CTGAGCTAAG GCCAGACTTC TTTGACTCTG CAGCTATCAT AGAAGATGAT TCAGGATTTG 
     4381 GAATGCCTAT GTTCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4441 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4501 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4561 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4621 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4681 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4741 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4801 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4861 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4921 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4981 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5041 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5101 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5161 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5221 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5281 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5341 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5401 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5461 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5521 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5581 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5641 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5701 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5761 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5821 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5881 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5941 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6001 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6061 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6121 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6181 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6241 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6301 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6361 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6421 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6481 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6541 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6601 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6661 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6721 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6781 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6841 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6901 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6961 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7021 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7081 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7141 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7201 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7261 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7321 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7381 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7441 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7681 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7741 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7801 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7861 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7921 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7981 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8041 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8101 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8161 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8221 ATTTAACAAA AATTTAACGC GAATTTT
//