LOCUS       RR207629                7317 bp    DNA     circular  SYN 
DEFINITION  Plg (Myc-DDK-tagged ORF) - Rat plasminogen (Plg), (10 ug)
ACCESSION   NM_053491
VERSION     NM_053491.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3474..3565 
                     /label="Myc-DDK"  
     misc_feature    3595..4180 
                     /label="PolyA signal"  
     misc_feature    3652..3671 
                     /label="Restriction Enzyme"  
     misc_feature    4456..5128 
                     /label="ColE1"  
     misc_feature    5558..6352 
                     /label="Kan/Neo"  
     misc_feature    6387..6689 
                     /label="SV40 ori"  
     misc_feature    6775..7252 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3456..3462 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3456 
                     /label="Plg"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCATAAG GAAATAATCC TTCTGTTTCT CTTGTTTCTG AAACCAGGAC 
     1081 AAGGGGACTC ACTGGATGGC TATGTAAGCA CACAGGGGGC TTCACTGCAT AGCCTCACCA 
     1141 AGAAGCAGCT AGCGGCAGGA AGCATAGCAG ACTGTTTGGC CAAATGTGAA GGGGAAACAG 
     1201 ACTTCATCTG CAGGTCATTC CAGTACCACA GCAAAGAGCA GCAGTGCGTG ATCATGGCCG 
     1261 AGAACAGCAA GACTTCCTCC ATCATCCGGA TGAGAGATGT CATCTTATTC GAAAAGAGAG 
     1321 TGTATCTGTC AGAGTGTAAG ACTGGCATCG GCAAGGGCTA CAGAGGAACC ATGTCCAAGA 
     1381 CAAAGACTGG TGTTACCTGT CAAAAGTGGA GTGACACGTC CCCCCACGTA CCCAAATACT 
     1441 CCCCCAGCAC ACACCCCAGC GAGGGACTAG AGGAGAACTA CTGTAGGAAC CCAGACAATG 
     1501 ATGAACAAGG GCCCTGGTGC TACACAACAG ACCCGGACCA GAGATATGAG TACTGCAACA 
     1561 TCCCCGAATG CGAAGAGGAA TGCATGTACT GCAGTGGAGA AAAGTACGAG GGCAAAATCT 
     1621 CCAAGACCAT GTCTGGACTC GACTGCCAGT CCTGGGATTC TCAGAGCCCA CATGCTCACG 
     1681 GATACATCCC TGCCAAATTT CCAAGCAAGA ACTTGAAGAT GAATTACTGT CGCAACCCTG 
     1741 ACGGGGAGCC GCGGCCCTGG TGCTTCACCA CAGACCCCAA CAAACGCTGG GAATACTGTG 
     1801 ACATCCCCCG CTGCACAACA CCCCCACCCC CACCGGGCCC AACCTACCAA TGTCTGAAGG 
     1861 GGAGAGGTGA AAATTACCGA GGGACCGTGT CTGTCACTGC GTCTGGGAAA ACCTGTCAGC 
     1921 GCTGGAGTGA GCAAACACCT CATAGGCACA ACAGGACGCC AGAAAACTTC CCCTGCAAAA 
     1981 ATCTGGAAGA GAACTACTGC CGGAACCCAG ATGGAGAAAC TGCTCCCTGG TGCTATACCA 
     2041 CTGACAGCCA GCTGAGGTGG GAGTACTGTG AGATTCCGTC CTGCGGGTCC TCGGTATCAC 
     2101 CAGACCAGTC AGATTCCTCA GTTCTTCCGG AGCAAACACC TGTGGTCCAG GAGTGCTACC 
     2161 AGGGCAATGG AAAGAGCTAT CGGGGCACAT CGTCCACTAC CAACACAGGG AAGAAGTGCC 
     2221 AGTCCTGGGT ATCTATGACT CCACATAGTC ACTCGAAGAC CCCAGCGAAC TTCCCAGATG 
     2281 CTGGCTTGGA GATGAACTAC TGCAGGAACC CAGATAATGA CCAGAGGGGC CCTTGGTGCT 
     2341 TCACCACTGA CCCGAGCGTC AGGTGGGAAT ACTGCAACCT GAAGCGGTGC TCGGAGACAG 
     2401 GAGGGGGTGT TGCGGAATCA GCCATAGTCC CCCAAGTTCC CAGTGCGCCA GGCACCTCTG 
     2461 AGACAGACTG CATGTATGGG AATGGCAAAG AATATCGGGG CAAAACGGCC GTCACTGCAG 
     2521 CTGGAACCCC CTGCCAGGAA TGGGCTGCCC AGGAGCCCCA CAGTCACAGA ATCTTCACCC 
     2581 CACAGACAAA CCCACGGGCA GGTCTGGAAA AGAATTACTG CCGAAACCCG GATGGGGATG 
     2641 TCAATGGGCC CTGGTGCTAT ACAATGAACC CCAGAAAACT TTACGACTAT TGTAACATTC 
     2701 CCCTTTGTGC ATCATTATCG TCCTTTGAAT GTGGGAAGCC TCAGGTGGAG CCGAAGAAAT 
     2761 GCCCTGGGAG GGTGGTGGGT GGCTGTGTGG CCAACCCTCA CTCCTGGCCC TGGCAAATCA 
     2821 GCCTTAGAAC AAGATTTTCT GGACAGCACT TCTGTGGCGG TACTTTAATA TCCCCAGAGT 
     2881 GGGTGCTGAC TGCCGCTCAC TGCTTGGAGA AATCTTCGAG ACCTGAATTC TACAAGGTTA 
     2941 TCCTGGGAGC ACACGAAGAA CGAATCCTTG GGTCAGATGT TCAGCAAATA GCAGTAACCA 
     3001 AACTGGTCTT GGAACCCAAC GACGCTGACA TTGCCCTGCT GAAGCTAAGC CGCCCAGCCA 
     3061 CCATCACAGA TAATGTCATC CCAGCTTGTC TGCCATCTCC AAATTATGTG GTTGCCGACC 
     3121 GGACACTGTG TTACATCACC GGCTGGGGAG AAACGAAAGG GACTCCAGGT GCCGGCCGTC 
     3181 TCAAGGAGGC CCAGCTGCCC GTGATCGAGA ACAAGGTGTG CAACCGCGCT GAGTATCTAA 
     3241 ACAACAGAGT CAAATCCACC GAGCTCTGTG CCGGGCATCT GGCTGGTGGC ATCGACAGTT 
     3301 GCCAGGGCGA CAGTGGAGGA CCTCTGGTTT GCTTCGAGAA GGACAAGTAT ATTTTACAAG 
     3361 GAGTCACTTC TTGGGGTCTT GGCTGTGCTC GCCCCAATAA GCCTGGTGTC TATGTTCGTG 
     3421 TTTCCCGGTA CGTTAATTGG ATTGAAAGGG AGATGAGGAA TGACACGCGT ACGCGGCCGC 
     3481 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3541 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3601 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3661 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3721 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3781 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3841 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3901 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3961 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4021 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4081 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4141 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4201 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4261 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4321 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4381 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4441 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4501 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4561 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4621 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4681 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4741 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4801 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4861 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4921 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4981 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5041 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5101 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5161 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5221 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5281 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5341 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5401 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5461 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5521 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5581 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5641 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5701 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5761 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5821 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5881 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5941 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6001 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6061 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6121 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6181 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6241 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6301 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6361 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6421 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6481 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6541 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6601 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6661 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6721 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6781 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6841 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6901 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6961 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7021 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7081 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7141 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7201 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7261 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//