LOCUS       RR207590                6499 bp    DNA     circular  SYN 
DEFINITION  Mgat3 (Myc-DDK-tagged ORF) - Rat mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), (10 ug)
ACCESSION   NM_019239
VERSION     NM_019239.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2656..2747 
                     /label="Myc-DDK"  
     misc_feature    2777..3362 
                     /label="PolyA signal"  
     misc_feature    2834..2853 
                     /label="Restriction Enzyme"  
     misc_feature    3638..4310 
                     /label="ColE1"  
     misc_feature    4740..5534 
                     /label="Kan/Neo"  
     misc_feature    5569..5871 
                     /label="SV40 ori"  
     misc_feature    5957..6434 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2638..2644 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2638 
                     /label="Mgat3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGATGAGA CGCTACAAGC TTTTTCTCAT GTTCTGTATG GCCGGCCTGT 
     1081 GCCTCATCTC CTTCCTGCAC TTCTTTAAGA CGTTATCCTA TGTCACCTTC CCGAGAGAAC 
     1141 TGGCCTCCCT CAGCCCTAAC CTCATATCCA GCTTCTTCTG GAACAATGCC CCTGTCACTC 
     1201 CCCAGGCCAG TCCGGAGCCC GGTGACCCCG ACTTGTTACG GACTCCACTC TACTCCCACT 
     1261 CCCCCCTGCT CCAGCCACTG TCCCCTAGCA AGGCCACCGA AGAACTGCAC CGGGTGGACT 
     1321 TCGTGTTGCC GGAGGACACC ACAGAGTATT TTGTGCGCAC CAAAGCTGGC GGTGTGTGCT 
     1381 TCAAACCAGG TACCAGGATG CTGGAGAAAC CTTCTCCAGG GCGGACAGAG GAGAAGACCA 
     1441 AGGTGGCTGA GGGGTCCTCG GTCCGGGGTC CTGCTCGGAG GCCTATGCGG CATGTGTTGA 
     1501 GTGCACGGGA GCGCCTGGGA GGCCGGGGCA CTAGGCGCAA GTGGGTTGAG TGTGTGTGCC 
     1561 TGCCAGGCTG GCACGGGCCC AGCTGCGGGG TGCCCACTGT GGTCCAGTAT TCCAACCTGC 
     1621 CCACCAAGGA GCGCCTGGTA CCCAGGGAGG TGCCGAGGCG GGTTATCAAC GCCATCAACA 
     1681 TCAACCATGA GTTCGACCTG CTGGATGTGC GCTTCCATGA GCTGGGCGAT GTTGTGGACG 
     1741 CCTTTGTGGT CTGCGAATCC AATTTCACCG CCTACGGGGA GCCTCGGCCG CTCAAGTTCC 
     1801 GAGAGATGCT GACCAATGGC ACCTTCGAGT ACATCCGCCA CAAGGTGCTC TACGTCTTCC 
     1861 TGGACCACTT CCCACCTGGT GGCCGTCAGG ACGGCTGGAT TGCAGACGAC TACCTGCGTA 
     1921 CCTTCCTCAC CCAGGATGGT GTCTCCCGCC TGCGCAACCT GCGACCTGAT GACGTCTTTA 
     1981 TCATCGACGA CGCGGACGAG ATCCCTGCGC GTGATGGTGT GCTGTTCCTC AAGCTCTACG 
     2041 ATGGCTGGAC AGAGCCCTTC GCCTTCCATA TGCGCAAGTC CCTGTATGGT TTCTTTTGGA 
     2101 AGCAACCAGG CACACTGGAG GTGGTGTCAG GCTGCACCAT TGACATGCTG CAGGCTGTGT 
     2161 ATGGGCTGGA CGGCATCCGC CTGCGCCGCC GTCAGTACTA CACCATGCCC AACTTTCGAC 
     2221 AGTATGAGAA CCGCACCGGC CACATCCTAG TGCAGTGGTC TCTCGGCAGC CCCCTGCACT 
     2281 TCGCGGGCTG GCACTGCTCC TGGTGCTTCA CACCCGAGGG CATCTACTTC AAACTCGTGT 
     2341 CGGCCCAGAA TGGTGACTTC CCCCGCTGGG GTGACTACGA GGACAAGAGG GACCTCAATT 
     2401 ACATCCGAAG CTTGATTCGC ACTGGGGGAT GGTTCGACGG CACGCAGCAG GAGTACCCTC 
     2461 CTGCAGACCC CAGTGAACAC ATGTATGCTC CTAAGTACCT GCTCAAGAAC TATGACCAGT 
     2521 TCCGCTACTT GCTCGAAAAT CCCTACCGGG AGCCCAAGAG CACTGTAGAG GGTGGGCGCC 
     2581 GGAACCAGGG CTCAGACGGA AGGTCATCTG CTGTCAGGGG CAAGTTGGAT ACAACGGAGG 
     2641 GCGGGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA 
     2701 AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT 
     2761 AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT 
     2821 GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT 
     2881 CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG 
     2941 CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG 
     3001 GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT 
     3061 CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT 
     3121 TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA 
     3181 ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC 
     3241 TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC 
     3301 ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC 
     3361 TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC 
     3421 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     3481 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     3541 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     3601 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     3661 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     3721 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     3781 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     3841 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     3901 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     3961 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     4021 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     4081 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     4141 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     4201 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     4261 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG 
     4321 CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG 
     4381 AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT 
     4441 GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT 
     4501 TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT 
     4561 CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA 
     4621 TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG 
     4681 TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA 
     4741 GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG 
     4801 AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC 
     4861 AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA 
     4921 GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC 
     4981 ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC 
     5041 GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC 
     5101 CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC 
     5161 CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG 
     5221 AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT 
     5281 TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA 
     5341 CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC 
     5401 AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT 
     5461 TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC 
     5521 GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT 
     5581 TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG 
     5641 AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG 
     5701 CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG 
     5761 ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC 
     5821 ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG 
     5881 GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT 
     5941 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT 
     6001 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT 
     6061 GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 
     6121 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT 
     6181 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT 
     6241 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG 
     6301 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 
     6361 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 
     6421 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 
     6481 AAAATTTAAC GCGAATTTT
//