LOCUS       RR207514                8628 bp    DNA     circular  SYN 
DEFINITION  Apaf1 (Myc-DDK-tagged ORF) - Rat apoptotic peptidase activating factor 1 (Apaf1), (10 ug)
ACCESSION   NM_023979
VERSION     NM_023979.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4785..4876 
                     /label="Myc-DDK"  
     misc_feature    4906..5491 
                     /label="PolyA signal"  
     misc_feature    4963..4982 
                     /label="Restriction Enzyme"  
     misc_feature    5767..6439 
                     /label="ColE1"  
     misc_feature    6869..7663 
                     /label="Kan/Neo"  
     misc_feature    7698..8000 
                     /label="SV40 ori"  
     misc_feature    8086..8563 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4767..4773 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4767 
                     /label="Apaf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGCAAAG GCCCGAAATT GTTTGCTTCA ACATAAAGAA GCTCTGGAAA 
     1081 AGGACATCAA AACATCCTAC ATCATGGATC ACATGATCAG TAATGGCGTC TTAACAGTAG 
     1141 TAGAGGAGGA AAAGGTCAAA AGTCAGGCCA CTCAATATCA ACGAGCAGCT GCTTTAATTA 
     1201 AAATGATACT TAATAAAGAC AATTATGCCT ACATTTCATT CTACAACGCT CTACTACACG 
     1261 AGGGCTATAA AGACCTTGCT GGACTTCTGC ATAGCGGCCT TCCTCTTGTG TCTTCTTCCA 
     1321 GCGGCAAGGA CACAGACGGT GGAAACACTT CATTTGTAAG GACAGTGCTG TGTGAAGGTG 
     1381 GAGTGCCACA GAGGCCAGTC ATCTTTGTCA CTAGGAAGAA GCTGGTCAGT GCGATTCAGC 
     1441 AGAAGCTCTG GAAACTGAAT GGGGAGCCAG GGTGGGTCAC CATCTATGGA ATGGCAGGCT 
     1501 GTGGCAAGTC TGTATTAGCT GCGGAAGCTG TTCGCGACCA TGCCCTCCTA GAAGGTTGTT 
     1561 TTTCAGGGGG CGTACACTGG GTTTCCATCG GAAAACAAGA CAAGTCCGGG CTTCTAATGA 
     1621 AGCTGCAGAA TCTTTGCACG CGGTTGGGTC AAGAAGAGAG TTTCTCTCAG AGGCTCCCGC 
     1681 TTAACATTGA GGAGGCCAAA GACCGTCTCC GTGTTCTGAT GCTGCGAAAA CACCCAAGGT 
     1741 CTCTGTTGAT CTTGGATGAC GTTTGGGATC CTTGGGTGTT AAAGGCTTTT GACAACCAGT 
     1801 GTCAGATTCT TCTTACCACC AGAGATAAGA GTGTTACCGA TTCAGTAATG GGTCCTAAGT 
     1861 ATGTTATCCC TGTGGAGAGT GGTCTAGGGA AAGAGAAGGG ACTTGAAATC TTGTCACTTT 
     1921 TTGTTAATAT GAAGAAAGAA GATCTGCCAG TGGAGGCTCA CAGTATTATA AAGGAGTGCA 
     1981 AAGGCTCTCC TCTCGTCGTG TCTTTAGTTG GTGCGCTTTT ACGGGATTTT CCCAATCGCT 
     2041 GGGCATACTA CCTCAGGCAG CTTCAGAATA AGCAGTTTAA GAGAATAAGG AAATCTTCAT 
     2101 CTTATGACTA CGAGGCTCTG GATGAAGCCA TGTCCATAAG TGTGGAGATG CTCAGAGAAG 
     2161 ACATCAAGGA CTACTACACA GACCTCTCCA TCCTTCAGAA GGATGTCAAG GTGCCTACGA 
     2221 AGGTGCTGTG TGTTCTCTGG GACTTGGAAA CGGAAGAAGT TGAAGACATC TTGCAGGAGT 
     2281 TCGTTAATAA GTCTCTCTTA TTCTGTAATC GGAATGGGAA GTCATTTTGT TATTATTTAC 
     2341 ACGATCTTCA AGTAGATTTC CTTACAGAGA AGAATCGAAG TCAGCTCCAG GATCTGCACA 
     2401 GGAAGATGGT CACTCAATTT CAGAGGTATC ACCAGCCGCA TACGCTGTCT CCAGGTCAGG 
     2461 AGGACTGCAT GTACTGGTAC AACTTCCTGG CCTATCATAT GGCCAGTGCC GGTATGCACA 
     2521 AAGAACTTTG TGCTTTAATG TTTTCCCTGG ATTGGATTAA AGCAAAAACA GAACTTGTCG 
     2581 GCCCTGCGCA TCTGATTCAT GAGTTCGTGG AATATAGGCA TATACTGGAT GAAAAGGATT 
     2641 GTGCAGTCTG TGAGAATTTT CAGGAGTTTT TATCTTTAAA CGGACACCTT CTTGGACGAC 
     2701 AGCCATTTCC TAACATTGTG CAACTGGGCC TCTGTGAACC AGAGACTTCC GAAGTTTATC 
     2761 AACAAGCAAA GCTGCAGGCC AAGCAGGAGG TGGATACCGG GCGGCTTTAC CTGGAATGGA 
     2821 TCAACAAAAA AACCATCAAG AATCTGTCCC GCTTAGTCGT TCGCCCCCAT ACAGACGCCG 
     2881 TGTACCATGC GTGTTTTTCT CAGGATGGTC AGAGAATAGC TTCTTGTGGA GCTGATAAAA 
     2941 CCTTGCAGGT GTTTAAAGCC GAGACAGGAG AGAAACTTCT TGACATTAAA GCTCATGAAG 
     3001 ATGAGGTGCT TTGCTGTGCG TTCTCCTCAG ATGACAGTTA CATAGCAACC TGCTCAGTGG 
     3061 ATAAGAAAGT TAAGATTTGG GATTCTGGGA CTGGGAAGCT AGTGCACACC TACGAAGAAC 
     3121 ACTCAGAGCA GGTCAATTGC TGCCATTTCA CCAATAAGAG CAACCACCTT CTTTTGGCCA 
     3181 CCGGGTCAAA TGACTCCTTC CTTAAGCTCT GGGATTTGAA TCAAAAAGAA TGCCGAAATA 
     3241 CCATGTTCGG TCACACGAAC TCAGTCACCC ACTGCAGGTT CTCACCAGAC GATGAGCTCT 
     3301 TGGCGAGTTG CTCAGCCGAC GGGACTTTAA AGCTTTGGGA TGTGAGATCA GCAAACGAGA 
     3361 AGAAAAGCAT TAATGTGAAG CGCTTCTTCC TGAGTTCAGA AGACCCACCA GAGGACGTGG 
     3421 AGGTGATCGT GAAATGTTGC TCCTGGTCTG CAGATGGCGA CAGAATAATA GTGGCAGCAA 
     3481 AAAACAAAGT CCTCCTTCTT GATATTCATA CTAGTGGCCT GCTGACTGAG ATCCACACAG 
     3541 GTCATCACAG CACCATCCAG TACTGTGACT TCTCCCCCTA CGACCATTTG GCAGTGATTG 
     3601 CCCTGTCTCA GTACTGTGTG GAGTTGTGGA ACATAGACTC CCGGGTAAAG GTGGCTGACT 
     3661 GCAGGGGACA TTTAAGTTGG GTCCACGGTG TGATGTTTTC TCCTGATGGC TCCTCGTTCT 
     3721 TGACAGCTTC TGATGACCAA ACAATAAGGG TCTGGGAGAC AAGAAAGGTG TGCAAGAACT 
     3781 CTGCCATTGT GTTAAAGCAG GAAATAGATG TCGTGTTTCA AGAAAACGAA ATGATGGTCC 
     3841 TTGCAGTTGA CAACATAAGA GGCCTACAAC TCATTGCTGG AAAAACAGGC CAGATTGATT 
     3901 ACCTGCCTGA AGCCCAAGTG AGTTGCTGCT GCCTAAGTCC ACATCTTGAG TACGTTGCAT 
     3961 TTGGAGATGA AGAGGGAGCC ATTAAGATTA TAGAACTTCC AAACAACAGA GTTTTCAGCT 
     4021 CTGGGATTGG GCACAAGAAA GCTGTACGGC ACATCCAGTT CACAGCTGAT GGGAAGACAC 
     4081 TGATTTCAAG TTCTGAAGAC TCTGTGATTC AGGTGTGGAA TTGGCAGACA GAGGAGTATG 
     4141 TATTTCTGCA AGCCCATCAG GAAACAGTAA AGGACTTCAG ACTCCTGAGA GATTCACGAT 
     4201 TGCTTTCCTG GTCATTCGAT GGAACGGTGA AGGTGTGGAA TGTCATTACC GGAAGAATTG 
     4261 AAAGAGACTT TACTTGTCAT CAGGGCACAG TGCTTTCCTG TGCTATCTCT TCTGATGCCA 
     4321 CCAAGTTTTC ATCTACCTCA GCTGATAAGA CCGCCAAGAT CTGGAGTTTT GAACTCCCTT 
     4381 CCCCTCTTCA TGAGCTGAAG GGCCATAACA GCTGTGTCCG CTGCTCTGCC TTCTCGCTGG 
     4441 ATGGCATCCT GTTAGCAACT GGAGATGACA ATGGAGAAAT CAGGATATGG AATGTCTCAG 
     4501 ATGGCCAGCT TCTTCATTTG TGTGCTCCGA TTTCCATAGA AGAAGGAACG GCTACCCACG 
     4561 GAGGCTGGGT GACTGATGTT TGCTTCTCTC CTGACAGAAA AATGCTTGTC TCTGCCGGAG 
     4621 GATACCTTAA GTGGTGGAAT GTTGTTACTG GGGAGTCCTC ACAGACCTTC TACACCAATG 
     4681 GAACAAACCT CAAGAAAATA CATGTGTCCC CTGACTTCAG AACATATGTG ACCGTTGATA 
     4741 ATCTTGGTAT TTTATATATT TTACAGGTTT TAGAGACGCG TACGCGGCCG CTCGAGCAGA 
     4801 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4861 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4921 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4981 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5041 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5101 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5161 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5221 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5281 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5341 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5401 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5461 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5521 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5581 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5641 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5701 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5761 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5821 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5881 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5941 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6001 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6061 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6121 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6181 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6241 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6301 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6361 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6421 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6481 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6541 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6601 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6661 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6721 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6781 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6841 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6901 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6961 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7021 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7081 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7141 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7201 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7261 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7321 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7381 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7441 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7501 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7561 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7621 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7681 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7741 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7801 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7861 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8041 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8101 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8161 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8221 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8281 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8341 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8401 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8461 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8521 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8581 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//