LOCUS       RR207506                8634 bp    DNA     circular  SYN 
DEFINITION  Ppfia2 (Myc-DDK-tagged ORF) - Rat protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (Ppfia2), (10 ug)
ACCESSION   NM_001108745
VERSION     NM_001108745.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4791..4882 
                     /label="Myc-DDK"  
     misc_feature    4912..5497 
                     /label="PolyA signal"  
     misc_feature    4969..4988 
                     /label="Restriction Enzyme"  
     misc_feature    5773..6445 
                     /label="ColE1"  
     misc_feature    6875..7669 
                     /label="Kan/Neo"  
     misc_feature    7704..8006 
                     /label="SV40 ori"  
     misc_feature    8092..8569 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4773..4779 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4773 
                     /label="Ppfia2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGCGAA GTGATGCCCA CGATTAATGA GGACACCCCA ATGAGCCAAC 
     1081 GGGGGTCCCA AAGTAGTGGC TCTGACTCGG ACTCCCACTT TGAGCAACTG ATGGTCAATA 
     1141 TGCTAGATGA AAGGGACCGT TTACTGGACA CACTTCGAGA GACTCAGGAA AGCCTCTCGC 
     1201 TTGCCCAGCA AAGACTCCAG GATGTCATCT ACGACCGAGA TTCACTCCAG AGACAGCTTA 
     1261 ATTCAGCCCT GCCTCAGGAC ATCGAATCTC TAACAGGAGG GCTGACTGGT TCTAAGGGGG 
     1321 CTGATCCACC GGAGTTTGCT GCACTGACTA AAGAGCTGAA TGCTTGCCGT GAGCAACTTC 
     1381 TAGAAAAGGA AGAAGAGATT TCAGAGCTAA AAGCTGAAAG AAACAACACA AGACTGTTGC 
     1441 TGGAACACTT GGAGTGCCTT GTGTCCCGAC ATGAAAGGTC ACTAAGAATG ACAGTGGTCA 
     1501 AGCGGCAAGC ACAGTCTCCC TCTGGAGTGT CCAGTGAAGT GGAGGTTCTC AAGGCACTGA 
     1561 AATCTTTGTT TGAGCACCAC AAAGCCTTGG ATGAAAAGGT AAGGGAGCGA CTGAGGGTTT 
     1621 CTTTAGAAAG AGTCTCTGCA CTGGAAGAGG AACTAGCTGC AGCTAATCAG GAGATTGTTG 
     1681 CCTTGCGTGA GCAAAATGTT CATATTCAAA GAAAAATGGT ATCGAGTGAG GGGTCCACGG 
     1741 AGTCAGAACA TCTTGACGGG ATGGAACCAG GCCAGAAAGT CCATGAAAAG CGTCTATCCA 
     1801 ATGGCTCCAT AGATTCCACA GATGACACCA GCCAGATCGT CGAGCTGCAA GAATTGCTTG 
     1861 AAAAGCAAAA CTATGAAATG GCCCAAATGA AAGACCGCTT AGCAGCTCTT TCTTCCCGAG 
     1921 TGGGAGAAGT GGAACAAGAA GCCGAGACGG CAAGAAAGGA CCTCATTAAA ACAGAAGAAA 
     1981 TGAACACCAA ATATCAAAGG GACATCCGAG AGGCCATGGC TCAGAAGGAA GATATGGAAG 
     2041 AAAGAATCAC AACTCTGGAG AAGCGTTACC TGAGTGCCCA GAGAGAATCT ACCTCCATTC 
     2101 ACGACATGAA TGATAAACTA GAAAATGAGT TGGCAAACAA GGAAGCCATC TTGCGGCAGA 
     2161 TGGAAGAGAA AAACAGGCAA TTGCAAGAGC GTCTTGAGCT GGCTGAGCAA AAGTTACAGC 
     2221 AGACCATGAG GAAAGCTGAA ACTTTACCTG AAGTAGAGGC AGAACTTGCT CAGAGAATTG 
     2281 CAGCCCTAAC AAAGGCTGAA GAGAGACATG GAAATATTGA AGAGCGTATG AGACACCTAG 
     2341 AGGGTCAACT TGAAGAGAAG AATCAAGAAC TTCAAAGAGC TAGGCAAAGG GAGAAAATGA 
     2401 ATGAAGAGCA TAACAAGAGA TTATCTGACA CCGTGGACAG GCTTCTGACA GAATCCAATG 
     2461 AGCGCCTGCA GCTGCACCTA AAGGAAAGGA TGGCTGCCTT GGAAGAGAAG AATGTTTTGA 
     2521 TTCAAGAATC AGAAACCTTC AGAAAAAATC TTGAAGAGTC CTTACATGAT AAGGAAAGAC 
     2581 TAGCAGAAGA AATAGAGAAG TTGAGGTCTG AACTTGACCA AATGAAAATG AGAACTGGCT 
     2641 CTCTAATTGA ACCAACTATA TCGAGAACTC ATCTAGACAC CTCCGCTGAG TTGCGGTATT 
     2701 CAGTTGGGTC CCTCGTAGAC AGCCAGTCTG ATTACAGAAC CACTAAAGTA ATACGAAGAC 
     2761 CAAGGAGAGG CCGCATGGGT GTGCGAAGAG ATGAGCCAAA GGTGAAATCC CTTGGGGATC 
     2821 ATGAATGGAA CAGGACTCAG CAAATTGGAG TACTAGGCAG TCACCCATTC GAAAGTGACA 
     2881 CTGAAATGTC AGATATTGAT GATGACGACA GAGAAACGAT TTTCAGCTCA ATGGATCTTC 
     2941 TCTCTCCAAG TGGCCATTCT GATGCCCAGA CGCTAGCTAT GATGCTTCAA GAACAACTGG 
     3001 ATGCAATCAA CAAAGAAATA AGGCTAATCC AGGAAGAAAA GGAATCTACA GAATTGCGTG 
     3061 CTGAAGAAAT CGAGAATAGG GTGGCTAGTG TAAGCTTGGA AGGTCTGAAC TTGGCAAGGG 
     3121 TCCATCCTGG TACCTCCATC ACTGCTTCTG TTACAGCTTC ATCACTGGCC AGTTCATCTC 
     3181 CCCCAAGTGG ACACTCAACG CCAAAGCTTA CACCAAGAAG CCCGGCCAGG GAAATGGACC 
     3241 GTATGGGAGT CATGACCCTG CCAAGTGATC TAAGGAAACA TCGGAGAAAG ATTGCAGTGG 
     3301 TGGAAGAAGA TGGGCGGGAA GATAAAGCAA CAATTAAATG TGAAACTTCT CCTCCCCCAA 
     3361 CACCCAGAGC CATCAGAATG ACTCACACCC TACCTTCTTC CTACCACAAT GAGGCCCGGA 
     3421 GCAGTTTATC TGCCTCTCTT GAGCCAGATA GACTTGGGCT TGGCAGTGCC AATAGCAGCC 
     3481 AGGACTCTCT TCACAAAGCC CCGAAGAAGA AAGGAATCAA GTCTTCAATT GGGCGTTTGT 
     3541 TTGGTAAAAA AGAGAAGGCT CGACTTGGGC AGCTTCGAGG CTTCATGGAG ACAGAAGCTG 
     3601 CAGCACAGGA ATCGCTGGGC TTAGGCAAAC TTGGCACTCA AGCTGAAAAG GACAGAAGGC 
     3661 TGAAGAAAAA GCATGAACTT CTTGAAGAAG CTCGTAGAAA AGGATTACCC TTTGCCCAGT 
     3721 GGGACGGACC TACTGTGGTT GCGTGGCTGG AGCTCTGGCT GGGAATGCCT GCTTGGTATG 
     3781 TGGCAGCATG CAGAGCCAAC GTGAAAAGTG GTGCCATCAT GTCGGCTTTA TCAGACACTG 
     3841 AAATCCAAAG GGAGATTGGA ATCAGCAACC CTCTGCACCG CTTAAAGCTC AGACTAGCCA 
     3901 TCCAGGAGAT GGTTTCCCTC ACAAGCCCTT CAGCTCCTCC GACATCGCGA ACTCCTTCAG 
     3961 GCAATGTCTG GGTGACCCAT GAAGAAATGG AAAACCTCGC AGCTCCAGCA AAAACGAAAG 
     4021 AATCTGAGGA AGGAAGCTGG GCCCAGACCC TGGCTTATGG AGATATGAAC CATGAGTGGA 
     4081 TCGGGAACGA ATGGCTTCCC AGCCTCGGAT TACCGCAATA CCGAAGTTAC TTTATGGAAT 
     4141 GCTTGGTTGA TGCAAGAATG TTAGATCACC TCACAAAAAA GGATCTTCGT GTCCATTTAA 
     4201 AAATGGTGGA TAGTTTCCAT CGAACAAGTT TACAGTATGG GATTATGTGC TTGAAAAGAT 
     4261 TGAATTATGA TAGGAAAGAG CTAGAGAGAA GACGAGAAGC AAGTCAACAT GAAATAAAAG 
     4321 ATGTTTTAGT GTGGAGCAAT GATCGAGTTA TTCGCTGGAT CCAAGCGATT GGACTTCGGG 
     4381 AATATGCCAA TAACATTCTT GAGAGTGGAG TGCATGGCTC ACTCATAGCC CTGGATGAGA 
     4441 ACTTTGACTA CAGCAGCCTA GCTCTACTGC TGCAGATTCC CACGCAGAAC ACCCAGGCAA 
     4501 GGCAGATTCT TGAACGCGAG TATAATAACC TCTTGGCTCT GGGAACTGAG CGGCGACTGG 
     4561 ATGAAAGTGA TGACAAGAAT TTCAGACGCG GATCAACCTG GCGAAGGCAG TTCCCTCCTC 
     4621 GCGAGGTACA CGGAATCAGC ATGATGCCTG GATCCTCAGA AACATTACCA GCTGGATTCA 
     4681 GGTTGACCAC AACATCTGGG CAGTCCAGAA AAATGACGAC AGATGTTGCT TCATCAAGAC 
     4741 TGCAGAGGTT AGACAACTCC ACTGTTCGCA CATACTCATG TACGCGTACG CGGCCGCTCG 
     4801 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4861 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4921 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4981 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5041 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5101 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5161 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5221 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5281 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5341 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5401 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5461 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5521 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5581 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5641 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5701 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5761 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5821 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5881 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5941 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6001 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6061 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6121 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6181 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6241 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6301 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6361 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6421 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6481 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6541 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6601 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6661 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6721 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6781 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6841 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6901 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6961 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7021 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7081 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7141 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7201 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7261 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7321 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7381 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7441 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7501 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7561 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7621 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7681 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7741 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7801 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7861 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7921 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7981 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8041 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8101 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8161 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8221 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8281 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8341 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8401 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8461 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8521 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8581 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//