LOCUS       RR207492                7260 bp    DNA     circular  SYN 
DEFINITION  Fgd1 (Myc-DDK-tagged ORF) - Rat FYVE, RhoGEF and PH domain containing 1 (Fgd1), (10 ug)
ACCESSION   NM_001037546
VERSION     NM_001037546.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3417..3508 
                     /label="Myc-DDK"  
     misc_feature    3538..4123 
                     /label="PolyA signal"  
     misc_feature    3595..3614 
                     /label="Restriction Enzyme"  
     misc_feature    4399..5071 
                     /label="ColE1"  
     misc_feature    5501..6295 
                     /label="Kan/Neo"  
     misc_feature    6330..6632 
                     /label="SV40 ori"  
     misc_feature    6718..7195 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3399..3405 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3399 
                     /label="Fgd1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGGGTG CTTCCTCCTC CTGAGCCCAT TCCTCCGCCA CCATCACGCC 
     1081 CTCTGCCTGC AGACCCTAGA GTGGCCAAGG GTCTTGCCCC CAGAGCTGAG GCCAGCACTA 
     1141 GTTCTGCAGC AGTATCATCA TTGATCGAGA AGTTTGAAAG AGAGCCTGTG ATTGTCGCTT 
     1201 CGGATAGGCC AGCCCCTGGC CCCTGCCCAG TTCCCCCAGA GCCAGCCATG TTGCCACAGC 
     1261 CACCCCCGCA GCCAACAGGG TCCCAGCTCC CTGAGGGTGA GGCTTCTCGC TGCCTGTTCC 
     1321 TGCTGGCTCC TGGGCCCCGG GATGGTGAGA AGGTGCCTAA CCGGGACAGC GGCATTGACA 
     1381 GCATCAGCTC GCCATCCAAC AGTGAAGAGA CCTGCTTCGT CAGTGATGAC GGGCCCCCCA 
     1441 GCCACAGCCT CTGTCCTGGG CCCCCTGCCC TGGCTAGTAT GCCAGTGGCT TTGGCCGACC 
     1501 CCCACCGGCC TGGCTCCCAG GAGGTTGACA GTGACCTGGA AGAGGAAGAG GAGGAGGAGG 
     1561 AAGAAGAGGA GGAAAAGGAA AGAGAAATTC CAGTGCCCCC GATGGAGAGA CAGGAGTCTG 
     1621 TGGAGCTGAC CGTGCAGCAG AAGGTATTCC ATATTGCCAA TGAACTCCTG CAAACTGAGA 
     1681 AGGCCTATGT TTCCAGGCTT CATCTCCTGG ATCAGGTATT CTGTGCCCGG CTGCTGGAAG 
     1741 AAGCTCGGAA CCGCAGTTCC TTCCCTGCAG ATGTTGTCCA TGGCATCTTC TCTAACATCT 
     1801 GCTCCATCTA CTGCTTCCAT CAGCAGTTCC TGCTGCCTGA GCTGGAGAAG CGCATGGAGG 
     1861 AATGGGACCG CTACCCACGC ATTGGTGACA TCCTTCAGAA GTTGGCACCC TTCCTCAAGA 
     1921 TGTATGGCGA GTATGTGAAA AACTTTGACC GGGCTGTAGA GCTGGTCAAC ACATGGACAG 
     1981 AGCGTTCTAC CCAGTTTAAA GTCATCATCC ATGAAGTACA GAAGGAGGAA GCCTGTGGCA 
     2041 ACCTGACCCT GCAGCACCAC ATGCTGGAGC CCGTGCAGCG TATCCCCCGC TATGAGCTTC 
     2101 TTCTTAAGGA CTATCTGTTA AAGCTGCCTC ATGGCTCCCC GGACAGCAAG GATGCCCAGA 
     2161 AGTCTTTGGA GCTGATAGCC ACAGCAGCAG AACACTCTAA TGCTGCCATC CGTAAAATGG 
     2221 AACGAATGCA CAAGCTGCTG AAGGTCTATG AGCTGCTAGG GGGTGAGGAG GACATTGTCA 
     2281 GTCCCACCAA AGAGCTCATA AAAGAAGGTC ATATCCTTAA GCTGTCAGCA AAGAATGGGA 
     2341 CCACTCAAGA TCGATACCTC ATATTATTCA ACGACCGCCT TCTTTACTGC GTGCCCAGGC 
     2401 TGCGGCTCCT TGGCCAGAAG TTTAGCGTGC GGGCACGCAT TGATGTAGAT GGCATGGAGC 
     2461 TAAAGGAAAG CTCCAACCTC AACATGCCTC GGACCTTCTT GGTGTCAGGA AAACAGCGCT 
     2521 CTCTTGAACT CCAGGCCAGG ACTGAGGAGG AGAAGAAAGA CTGGGTCCAG GCCATCAATT 
     2581 CTACCCTCCT GAAGCATGAA CAGACCCTGG AGACTTTCAA ACTGTTGAAC TCAACAAACA 
     2641 GGGATGATGA AGATACTCCC CCTAACTCTC CAAACGTGGA TCTTGGGAAG AGGGCACCTA 
     2701 CGCCTATCCG GGAAAAGGAA GTCACCATGT GCATGCGCTG CCAGGAGCCC TTCAATTCCA 
     2761 TCACCAAACG CAGGCACCAC TGCAAGGCCT GTGGGCATGT GGTTTGTGGG AAATGCTCTG 
     2821 AGTTCCGAGC ACGACTCATC TATGACAACA ATCGTTCCAA CCGTGTGTGC ACTGATTGCT 
     2881 ATGTGGCCTT GCATGGAGCA CCCGGGAGCA GTCCAGCCTG TAGCCAACAT ACACCCCAGC 
     2941 GCAGGAGGTC CATCCTAGAG AAACAGGCCT CAGTGGCTGC TGAGAACAGC GTCATCTGCA 
     3001 GCTTCCTGCA CTACATGGAG AAGGGTGGGA AAGGATGGCA CAAGGCATGG TTTGTGGTTC 
     3061 CCGAGAATGA ACCCTTGGTG CTGTACATCT ATGGAGCCCC TCAGGATGTG AAAGCCCAAC 
     3121 GCAGCCTGCC CCTCATTGGC TTTGAGGTGG GGCCTCCTGA GGCAGGGGAG CGGCCAGACA 
     3181 GGAGGCATGT CTTCAAGATC ACCCAGAGCC ACCTAAGCTG GTACTTCAGC CCAGAGACAG 
     3241 AAGAACTCCA GCGGCGCTGG ATGGCTGTGC TTGGCAGGGC GGGCCGTGGG GACACATTCT 
     3301 GCCCAGGGCC CACACTGTCT GAGGACAAGG AGATGGAGGA GACAACAGTG GCAGCTTCAG 
     3361 GAGCCACTGC TGAACCTCCT GAAGCCTCCC AGACCCGAGA CAAGACCACG CGTACGCGGC 
     3421 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3481 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3541 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3601 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3661 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3721 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3781 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3841 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3901 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3961 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4021 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4081 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4141 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4201 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4261 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4321 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4381 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4441 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4501 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4561 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4621 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4681 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4741 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4801 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4861 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4921 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4981 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5041 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5101 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5161 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5221 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5281 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5341 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5401 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5461 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5521 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5581 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5641 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5701 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5761 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5821 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5881 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5941 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6001 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6061 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6121 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6181 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6241 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6301 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6361 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6421 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6481 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6541 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6601 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6661 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6721 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6781 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6841 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6901 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6961 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7021 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7081 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7141 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7201 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//