LOCUS       RR207475                8919 bp    DNA     circular  SYN 
DEFINITION  Gprasp1 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor associated sorting protein 1 (Gprasp1), (10 ug)
ACCESSION   NM_134386
VERSION     NM_134386.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5076..5167 
                     /label="Myc-DDK"  
     misc_feature    5197..5782 
                     /label="PolyA signal"  
     misc_feature    5254..5273 
                     /label="Restriction Enzyme"  
     misc_feature    6058..6730 
                     /label="ColE1"  
     misc_feature    7160..7954 
                     /label="Kan/Neo"  
     misc_feature    7989..8291 
                     /label="SV40 ori"  
     misc_feature    8377..8854 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5058..5064 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5058 
                     /label="Gprasp1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGGGGCA GAGGTTGAGC CTGGTGCCCA GGCAAAGGCT GAAAATAAGC 
     1081 CTGGGGATGA GAATGCTAAT GCTGCTGAAG TAGAGCCTGA AGTTCCGTTA GTGGTCAGAC 
     1141 CCAAGGTTAG GACACAAATG ATGACTGGAG CAAGACCCAA AGTGAAGCCT AAGGGTACCC 
     1201 CTGGAGCAAG GCCTAAAGGT GAAACCAGTT CACCAGGTGG GGCATATGCT AAATGTAAGC 
     1261 CCAGGTCAAT CCCTATTTCA CGATCTAAGC ATGATGCACA AGTCTGGGCC CCGAGTAAGT 
     1321 TTCGTGGTGA GTCCATGTCA AAGATGGGGA AGCAATGTCA GATCAGTGCT GCAGACTCCC 
     1381 CACTGGTCAG TAATGATTCT GGGGCGGTTG CTCAAGCTAA GTGCCTGTCT GTAGATAGAG 
     1441 AACTTGCTAA TATGGACACT GAGAGTATTC CCAAAAAGGC CAGTTCTCCA GCAAGATTCC 
     1501 AGCCTTCATT TGGCCCAGAG GAGGGAACCA GTATGGGATC CTGGTACCGT CCCAGGCCTA 
     1561 TCCCTAAAGG AGAGGCATAT GAGAATTCTG ATTTCAAATG GGCAGATAAA TCCTCAGGAA 
     1621 GTTCCTCCTT CTGGAATAGA GATGAAACCA GTACAAGATT TCGTCCTAGG AAAAGCATGA 
     1681 AATCCAATAC CAGATTCAGG CACATGGCCA AACAAGAGGC AAATACCATG TCTAGACACA 
     1741 AGAACAAACA GGAGTTCTAT AATATTTCCA GTTCTGATTC TGAAGATGAG TCTGCTAAGA 
     1801 CTCCCTGGTT CTGGGCCAAA GACAAGCCCA AAGTCTGGTC TAGGCCCAAA GAAGAGCCCA 
     1861 ATACTAGGTC CTGGTTTAGG TCTAAGAAAG AAGTCCGTGT TGAATCCACT TCTGGGTCTG 
     1921 AATGTGAAAA CCATACAAAA TCTTTGTTTT GGTCTGGAGA AGAGGCTAAA TGTAGGTCTA 
     1981 AACCCAGAGC CAGGAAAGGG GTCAATATGA GGGCCAGGCA CCAGGCCAAG CGAGAAGCTT 
     2041 ATAGTGATGT CACATCTGGA TCTGTAGATA AAAATAAGAA AGATTCCTGG TTCTTGCCTG 
     2101 AAGAGAAAGC TAATGCCTTT TCAAAGTCTA AGACAAAGAA AGAGCCCAGA ACCAGAGCAA 
     2161 TGCCAAGGGA AGAAGTCAAA ACTAAGGCCA GAGCAAGTAC CAAACAAGAA GCCAGGCCAG 
     2221 AGGAGGAAGT CCTCGTTGGG GCTTGGGTTC TGGACACCCA GGACAACACT ATGGGGGAGA 
     2281 GGATAAGCAT GAAGACCACT TGTGTAGAGG AGGAGCCTAT TGTTGGAGAT TGGTTCTGGA 
     2341 GTGAAGAAGA AGCCAGTGTG GACTCTGAGA CCGGTCTTAA ATCCAGACCA AGGGCTAAGG 
     2401 AGGAACAAGT TAGTAGTTTC TGTTTAGGAT CTGGAAAGAA GACCAGTATG GAAAGTGGGC 
     2461 CAAAGGCTAC TTCTAAATCT ATGCCAGTAG CTAAGGATGA TGAGGTCATT ATTGGATCCT 
     2521 GGTTCTGGGC TGATGATGAA GAGATCAGCT TACAGGCTGA TGATGAGTCT ATTTTTGGAT 
     2581 CTTGGTTCTG GGGCACTGGT GAGAAAAGCC TTAGATCTGT TGGAGTCAGT TGTGAGAAGA 
     2641 TGCCCAAGTC TGGGGAAAAA GAAGTTACTG ATTCCTGGTT CTGGGCTGGA GAAGTCAATA 
     2701 CTGAGGCTGA GATGGAGGAA CAGGCCAGTT CAGCATCCAC AAAAGGAACA ATCTTTGTGC 
     2761 CTTGGTTTTG GTCTGAAAAG CAAGCACATA TGGATTTAGG AACCGAACCT TGCTCTGACA 
     2821 TCATGGCAGG AGCTGAGGAG GAGCCCATAA TTGGGCCCTG GTTCTGGGCT AAAGTAGATA 
     2881 ATAGTGTGGA GGCTGAAGTT AATAGTAAGT CTAGCCTTGA AGATGAGGAA GAACCCATTA 
     2941 GATCACCTTG GTTTGGGGCC AGAGAACAAC CCAATATGAA GTATGCAGCT GGTGTCGGAT 
     3001 ACAAGCCTAT GGCAGAAGCC GAGGAGGCTA ACAAAAAGTC GTGTGTCTGG GCAAAAGAGC 
     3061 CCTGTTTGTA TCCTACCAAT AGAGAAAGCT TGAAATCTAC TCTGGGAGAG AAAGAGGACA 
     3121 CTGTTGATCC ATGGCTCTGG TCCAATAATT ACCCAAGGAC CGAGACCATC ACAGGGTCCT 
     3181 GGCTCTGGGC TGCAGAAGAG GGAAATATAG ATGATGAGAC TGGAGAAGAA ATCAAACTAC 
     3241 CAACTTTAGA GGACAATGTA TTCAATTCTT GGTCCTGGAA AGAAAATGAA GAAACCGTTG 
     3301 TAGAGGCTCC CAACAGAGAA GAGTCCAAGC CAGAAGCGGA AGAGGAAGAT ATTATTGGCT 
     3361 CTTGGTTTTG GGCTGGGGAT GAAGACAGGT TTCAGCCAGC TGCTAAGATT AAGGAAGAGA 
     3421 ACAAGATAGC ACCTGAAGAT GAAGATACAG TTGGATCCTG GTTCTGGGGC AAGGAAGAGG 
     3481 CCAGTGTAGA GGCAGTGAAA GGGGGTACTT TTGAGTCTGT GTCTGGAATT AAGGAAGAGA 
     3541 AAGCTACTGG GTCGTGGTTC TGGACTGATA AGGCCAAAAT AGGGGCTGGG CCACAGACTG 
     3601 TCGAAACTGG GTCAGAAACT GAAGATGAGG CAATTTTTGA GTCCCTGATC TGGGCCGCAA 
     3661 AAAAGGACAG TATGCAAACA GGAGTAAACC GTGTGTCCAA GCCAAAGGAT GAGGGTGAAG 
     3721 GGATTGAGTC CTGGCTCTGG TCTGGTGACA AGGCCACTAC AGAATCTAAA ACTGTGACGG 
     3781 TCAGTGAGTC TAGTCCAGAA AATGGGAAAG AGTCCATTGT TAAGTTTGGG TCAAGAGCAA 
     3841 AGGATGAAGT GATTAACAAG ACTGGCAGCG GTGACAATTG TAAATTTAGT ACAGAAGCTG 
     3901 AAAGCATAGT GGGACCCTGG TTCTGGGAAG GAGATGAAGC TAGCTTTGAA TCAAACCCTG 
     3961 TTCCTGTATG CAAGGCCGCT TGTGAACCTG AGTCTTCAAC TGAACATGAA CCTGATCCTT 
     4021 CCCGCAGGCC TCAGAGCTGG GATGAGGTCA CTGTTCAGTT TAAGCCTGGT CCATGGGGAA 
     4081 AGGCTGGCTT CCCATCCTTA AACCCCTTCC GATTCCCAAA AGAAGCAGCA TCTCTGTTTG 
     4141 CAGAAATGTT TGGGGGAAAG CCTAAGCTTG TGGAAGTTGG TACTGAAAGA GAGCCTGAAC 
     4201 CCCAGTTCCC ATTTCAGTAT GATCCTTCTT ACCGGTCAGT CCGGGAAATT CGGGAGCATC 
     4261 TTAAGGCCAG GGAGAGTGCA CAGGCTGAGA ACTGGTCTTG CAACTGCATC CAGTGTGAGC 
     4321 TTAGAATTGG TTCTGAGGAG TTTGAGGAAC TTCTTTTAAT GATGGACAGA AATCGTGATC 
     4381 CATTTATCCA TGAGATATCT AAAATTGCAA TGGGCATGAG AGGTGCTTCT CAGTTTACCC 
     4441 GAGATTTCAT TCGAAACTCG GGTGTTATCT CACTTATTGA AGCCCTGATG AATTATCCTT 
     4501 CCTCCCGAGC GAGGACTGCC TTTTTGGAAA ACATGATTCA AATGGCTCCA ACTTATCCAG 
     4561 ACCTAAACAT GATCGAGACA TATGTGTGTC AGGTGTGTGA GGATACCTTT GATTATGACT 
     4621 TGGATTCCTC TGACCAGTTG TCTGGACTGA CAATGATTAC ACACCTCACT ACTACTTTTG 
     4681 ACTATCACAA AGTGGTTGTC GCTTACTTGG CTGGCTTTTA CTACCTACTG AATTCGGGCA 
     4741 ATACCACAAC AAGGTTTCAT GTTCTGAAAC TGCTACTAAA TTTGTCTGAA AGCCTTGTCA 
     4801 TGACAAAACG CCTACTTATT ACTGATTCAG TGTCAGAATT TATGGCCCTT TTTAATAGGG 
     4861 AGGACTCAGA TGAAAACATT CAAATTATTC TTGCAATATT TGAGAATATC AGTAAAAATA 
     4921 TTCAAAAAGA GGCACTGTTT GCTGATGATG AGGAAGAGGA GGAGGAGGAG GAGGCGGTGA 
     4981 ATCTTGAACC GCTTATTTCA GCATTCCGTG AGGCTGAAAA ATTTGCCAAG GAACTGAAAC 
     5041 GTAAAACCGA CGATCAGAAA TCACCCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5101 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5161 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5221 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5281 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5341 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5401 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5461 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5521 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5581 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5641 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5701 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5761 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5821 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5881 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5941 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6001 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6061 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6121 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6181 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6241 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6301 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6361 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6421 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6481 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6541 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6601 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6661 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6721 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6781 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6841 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6901 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6961 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7021 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7081 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7141 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7201 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7261 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7321 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7381 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7441 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7501 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7561 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7621 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7681 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7741 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7801 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7861 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7921 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7981 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8041 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8101 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8161 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8221 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8281 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8341 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8401 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8461 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8521 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8581 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8641 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8701 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8761 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8821 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8881 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//