LOCUS       RR207434                8709 bp    DNA     circular  SYN 
DEFINITION  Scap (Myc-DDK-tagged ORF) - Rat SREBF chaperone (Scap), (10 ug)
ACCESSION   NM_001100966
VERSION     NM_001100966.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4866..4957 
                     /label="Myc-DDK"  
     misc_feature    4987..5572 
                     /label="PolyA signal"  
     misc_feature    5044..5063 
                     /label="Restriction Enzyme"  
     misc_feature    5848..6520 
                     /label="ColE1"  
     misc_feature    6950..7744 
                     /label="Kan/Neo"  
     misc_feature    7779..8081 
                     /label="SV40 ori"  
     misc_feature    8167..8644 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4848..4854 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4848 
                     /label="Scap"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCCTGACT GAAAGGCTTC GTGAGAAGAT ATCTCAGGCG TTCTACAACC 
     1081 ATGGGCTGCT CTGCGCATCC TACCCCATTC CCATCATCCT CTTCACAGGA CTCTGCATCC 
     1141 TAGCCTGCTG CTACCCGCTG CTGAAGCTTC CCTTGCCTGG AACGGGACCC GTGGAATTCT 
     1201 CCACGCCTGT GAAGGGTTAC TCGCCCCCGC CTGCGGACTC TGACCACAAA CAAGGAGAGC 
     1261 CCAGTGAGCA GCCAGAGTGG TATGTGGGTG CCCCCGTGGC ATACATCCAG CAGATATTCG 
     1321 TGAAGTCATC AGTGTCTCCC TGGCACAGAA ACCTTCTGGC AGTAGATGTG TTCCGGTCAC 
     1381 CTCTGTCCCG AGCATTCCAA CTGGTGGAAG AGATCCGGAA CCATGTGCTG AGAGACAGCT 
     1441 CAGGGACCAA GAGCCTGGAG GAAGTTTGCC TGCAGGTGAC AGACCTGCTG CCAGGCCTCA 
     1501 GGAAACTCCG GAGCCTACTT CCCGAACATG GCTGCCTGCT GCTGTCACCT GGGAACTTCT 
     1561 GGCAGAATGA CTGGGAAAGA TTCCATGCTG ACCCTGACAT CATTGGAACC ATCCATCAGC 
     1621 ATGAGCCTAA AACCCTACAG ACATCAGCCA CACTCAAAGA CTTGCTGTTC GGTGTTCCTG 
     1681 GGAAGTACAG TGGGGTCAGC CTCTACACGA GGAAGAGGAT GGTCTCATAC ACCATCACCC 
     1741 TGGTCTTCCA GCGCTACCAT GCCAAGTTTC TGAGCAGCCT CCGTGCCCGG CTCATGCTTC 
     1801 TGCACCCCAG CCCCAACTGC AGCCTCCGAG CAGAGAACCT GGTGCATGTG CACTTCAAAG 
     1861 AGGAGATTGG CATTGCCGAG CTCATCCCCC TCGTGACCAC CTACATCATC CTGTTTGCCT 
     1921 ACATCTACTT CTCCACACGC AAGATCGACA TGGTCAAGTC CAAGTGGGGC CTCGCCCTGG 
     1981 CAGCCGTGGT CACAGTGCTT AGCTCGCTGC TCATGTCTGT GGGGCTCTGC ACTCTCTTCG 
     2041 GCCTGACGCC CACACTCAAT GGCGGCGAGA TTTTCCCATA TCTGGTGGTG GTTATTGGGC 
     2101 TAGAGAATGT GTTGGTGCTC ACCAAGTCAG TGGTATCAAC TCCAGTGGAC CTTGAGGTGA 
     2161 AGCTTCGAAT TGCACAAGGC TTAAGCAGTG AGAGCTGGTC CATCATGAAG AACGTAGCAA 
     2221 CTGAACTGGG CATCATCCTC ATTGGCTACT TCACCCTTGT GCCTGCCATC CAAGAGTTCT 
     2281 GCCTCTTTGC TGTGGTGGGC CTGGTGTCTG ACTTCTTCCT CCAGATGCTG TTCTTCACCA 
     2341 CCGTGCTGTC CATCGACATT CGCCGGATGG AGCTAGCAGA CCTGAACAAG CGGCTGCCCC 
     2401 CTGAGTCCTG CCTGCCCTCA GCCAAGCCTG TGGGGAGGCC AGCCCGATAT GAGAGACAGC 
     2461 TAGCTGTACG GCCGTCCACA CCACACACCA TCACATTGCA ACCATCTTCC TTCCGAAACC 
     2521 TGCGGCTTCC CAAAAGGCTG CGTGTCATCT ACTTCCTGGC CCGCACTCGC CTGGCACAGC 
     2581 GCCTCATCAT GGCTGGTACA GTTGTCTGGA TTGGCATCCT GGTATATACA GACCCGGCAG 
     2641 GGCTGCGCAC CTACCTCGCT GCCCAGGTGA CAGAACAGAG CCCACTGGGT GAGGGTTCCC 
     2701 TGGGGCCCAT GCCTGTGCCT AGTGGAGTGC TGCCTGCCAG CCACCCGGAC CCTGCCTTCT 
     2761 CCATCTTCCC ACCTGATGCT CCTAAACTGC CAGAGAACCA GACGTTGCCA GGTGAGCTGC 
     2821 CTGAGCATGC CGTTCCAGCA GAGGGCGTCC AGGACAGCCG AGCCCCAGAG GTGACTTGGG 
     2881 GGCCCGAGGA TGAGGAGCTG TGGAGGAAAT TGTCCTTCCG CCACTGGCCC ACACTCTTCA 
     2941 ACTACTATAA TATCACACTG GCCAAAAGGT ACATCAGCCT GCTGCCTGTC ATCCCTGTCA 
     3001 CACTACACCT GAATCCACGG GAGGCTCTGG AGGGGCGACA CCCTCAGGAT GGCCGCACTG 
     3061 CCTGGGCCCC ACCAGAGCCT TTGCCTGCTG GCCTGTGGGA GACCGGACCT AAGGGGCCAG 
     3121 GTGGAACACA GACCCATGGC GACATTACCT TGTACAAGGT GGCTGCACTT GGCCTGGCAG 
     3181 CGGGCATTGT CCTAGTGCTG CTGCTGCTCT GCCTCTACCG GGTGCTCTGC CCGCGAAACT 
     3241 ACGGGCAGCC GGGTGGTGGT GCGGGCAGGC GGAGGCGCGG AGAGCTGCCT TGCGATGACT 
     3301 ATGGCTACGC ACCGCCTGAG ACGGAGATAG TGCCGCTGGT GCTGCGAGGG CACCTCATGG 
     3361 ACATCGAGTG TCTGGCTAGC GATGGGATGC TCCTGGTGAG CTGCTGCCTG GCTGGCCAAG 
     3421 TCTGCGTGTG GGATGCACAG ACCGGGGACT GCCTCACTCG CATCCCGCGC CCTGGGCCAC 
     3481 GCCGGGACAG CTGCGGAGGC GGAGCTTTTG AAGCTCAGGA GAACTGGGAA AGACTGTCTG 
     3541 ATGGGGGCAA AGCTAGCCCG GAAGAGCCTG GCGACAGCCC TCCGCTGCGA CGCCGCCCTC 
     3601 GAGGGCCTCC ACCGCCTTCC CTCTTTGGGG ACCAGCCAGA CCTCACCTGC TTAATCGACA 
     3661 CCAACTTCTC AGTGCAGCTG CCCCCAGAGC CCACTCAGCC CGAGCCTCGG CACCGGGCGG 
     3721 GCTGTGGCCG CTCTAGAGAC TCTGGTTACG ACTTCAGCCG TCTGGTGCAG CGTGTGTACC 
     3781 AAGAGGAAGG CCTGGCTGCT GTGCACATGT CGGCCCTGCG CCCACCCTCC CCGGGACCTC 
     3841 CCCTGCCCCA GGCCTCTCAA GAAGAGGGGA CTGCTCCCGA GAAGGGCTCC CCCCCTCTGG 
     3901 CCTGGGCCCC CAGCACAGCC GGTTCCATCT GGAGCTTAGA GTTGCAAGGC AGTCTCATCG 
     3961 TGGTTGGGCG AAGCAGCGGC CGGCTGGAGG TGTGGGATGC CATTGAGGGC GTGCTCTGCT 
     4021 GCAGCAATGA GGAGATCTCC TCAGGCATCA CAGCCCTTGT CTTCTTAGAC AGGAGGATTG 
     4081 TAGCTGCTCG GCTCAACGGT TCCCTCGATT TCTTTTCCTT GGAGACCCAC ACTTCCCTCA 
     4141 GCCCCCTGCA GTTCAGAGGG ACCCCAGGGA GAGGCAGTTC TCCTTCCTCG CCTGTGTACA 
     4201 GCAGCAGCAA CACTGTGGCC TGTCACCTGA CCCACACAGT CCCCTGTGCA CACCAGAAAC 
     4261 CCATCACAGC CCTGAGAGCA GCAGCGGGGC GCCTGGTGAC AGGGAGCCAA GACCATACTC 
     4321 TGAGAGTCTT CCGACTGGAG GATTCGTGTT GCCTCTTTAC CCTGCAGGGC CACTCGGGGG 
     4381 CAATCACAAC TGTGTACATT GATCAGACCA TGGTATTGGC CAGTGGAGGA CAAGATGGAG 
     4441 CCATCTGCCT GTGGGATGTA CTAACAGGCA GCCGGGTCAG CCATACATTT GCTCACCGTG 
     4501 GAGATGTCAC CTCCCTCACC TGTACCACTT CCTGTGTTAT CAGTAGTGGC CTGGATGACT 
     4561 TCATCAACAT CTGGGACCGA AGCACAGGCA TCAAGCTGTA CTCCATTCAG CAGGACCTGG 
     4621 GCTGTGGTGC AAGCTTGGGT GTCATCTCTG ATAACCTTCT GGTGACCGGC GGCCAGGGAT 
     4681 GTGTCTCCTT TTGGGACCTA AACTATGGGG ACCTGTTACA GACAGTCTAC TTGGGAAAGA 
     4741 ACAGTGAAGC CCAGCCTGCC CGGCAGATTT TGGTGCTGGA CAATGCTGCC ATTGTCTGCA 
     4801 ACTTTGGCAG TGAGCTCAGC CTAGTGTATG TGCCCTCTGT GCTGGAGAAA CTGGACACGC 
     4861 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4921 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4981 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5041 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5101 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5161 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5221 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5281 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5341 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5401 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5461 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5521 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5581 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5641 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5701 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5761 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5821 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5881 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5941 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6001 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6061 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6121 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6181 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6241 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6301 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6361 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6421 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6481 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6541 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6601 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6661 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6721 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6781 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6841 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6901 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6961 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7021 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7081 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7141 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7201 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7261 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7321 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7381 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7441 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7501 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7561 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7621 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7681 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7741 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7801 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7861 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7921 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7981 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8041 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8101 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8161 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8221 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8281 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8341 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8401 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8461 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8521 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8581 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8641 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8701 GCGAATTTT
//