LOCUS       RR207386                7662 bp    DNA     circular  SYN 
DEFINITION  Slc8a3 (Myc-DDK-tagged ORF) - Rat solute carrier family 8 (sodium/calcium exchanger), member 3 (Slc8a3), (10 ug)
ACCESSION   NM_078620
VERSION     NM_078620.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3819..3910 
                     /label="Myc-DDK"  
     misc_feature    3940..4525 
                     /label="PolyA signal"  
     misc_feature    3997..4016 
                     /label="Restriction Enzyme"  
     misc_feature    4801..5473 
                     /label="ColE1"  
     misc_feature    5903..6697 
                     /label="Kan/Neo"  
     misc_feature    6732..7034 
                     /label="SV40 ori"  
     misc_feature    7120..7597 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3801..3807 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3801 
                     /label="Slc8a3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTGGTTA CGGCTGCAGC CTCTCACCTC TGCCTTCCTC CATTTCGGGC 
     1081 TGGTTACTTT TGTGCTCTTC CTGAATGGTC TTCGAGCAGA GGCTGGTGAC TTGAGGGATG 
     1141 TGCCCAGTGC AGGACAGAAC AATGAGTCCT GTTCAGGGTC ATCAGACTGC AAGGAGGGTG 
     1201 TCATCTTGCC AATCTGGTAT CCAGAGAACC CTTCCCTTGG GGACAAGATT GCAAGGGTCA 
     1261 TTGTCTATTT TGTGGCCCTG ATATACATGT TTCTTGGAGT GTCTATCATT GCTGACCGAT 
     1321 TCATGGCATC TATTGAAGTT ATTACCTCCC AAGAGAGGGA GGTGACAATC AAGAAGCCCA 
     1381 ATGGAGAGAC CAGCACAACT ACAATTCGGG TATGGAATGA AACTGTCTCC AACCTGACCC 
     1441 TGATGGCCCT AGGCTCTTCT GCTCCGGAGA TTCTCCTGTC TTTAATTGAG GTGTGTGGTC 
     1501 ACGGGTTCAT TGCTGGTGAT TTGGGACCGT CTACCATTGT CGGCAGTGCA GCCTTCAACA 
     1561 TGTTCATCAT CATTGGCATC TGTGTCTATG TGATCCCAGA TGGGGAGACT CGCAAGATCA 
     1621 AGCACCTTCG AGTCTTCTTT GTCACGGCTG CTTGGAGCGT CTTTGCCTAT ATTTGGCTCT 
     1681 ACATGATCCT GGCAGTCTTC TCTCCTGGTG TGGTCCAGGT TTGGGAAGGC CTCCTTACTC 
     1741 TCTTCTTCTT TCCAGTGTGT GTCCTGCTGG CTTGGGTGGC AGATAAGCGA CTGCTCTTCT 
     1801 ACAAATACAT GCACAAAAGA TACCGCACAG ATAAACACCG AGGAATTATC ATTGAGACAG 
     1861 AGGGTGAACA CCCTAAGGGC ATTGAGATGG ATGGGAAAAT GATGAATTCT CACTTCCTGG 
     1921 ATGGGAACCT TATACCCTTG GAAGGAAAGG AGGTAGATGA ATCTCGCAGG GAAATGATCC 
     1981 GAATTCTTAA GGATCTGAAG CAAAAACACC CAGAAAAAGA CTTAGATCAG CTGGTGGAGA 
     2041 TGGCCAATTA CTATGCTCTT TCCCACCAAC AGAAGAGCCG TGCTTTCTAC CGCATCCAAG 
     2101 CCACCCGGAT GATGACTGGT GCAGGCAATA TACTTAAGAA ACATGCAGCA GAGCAAGCCA 
     2161 AGAAGACCGC CAGCATGAGT GAGGTGCATA CTGATGAGCC TGAGGACTTT GCCTCGAAGG 
     2221 TCTTTTTTGA CCCATGCTCT TACCAGTGCC TGGAGAACTG TGGAGCTGTC CTCCTGACTG 
     2281 TGGTGAGGAA AGGGGGAGAT ATATCCAAGA CTATGTACGT GGACTACAAA ACAGAGGACG 
     2341 GCTCTGCCAA TGCAGGGGCT GACTATGAGT TCACAGAAGG CACTGTGGTT CTGAAGCCAG 
     2401 GAGAGACCCA GAAGGAGTTC TCCGTGGGCA TCATCGATGA TGACATTTTT GAAGAGGATG 
     2461 AACACTTCTT CGTGAGACTG AGCAATGTCC GTGTAGAAGA GGAGCAGCTG GAAGAGGGGA 
     2521 TGACCCCCGC CATCCTCAAT AGTCTTCCTT TGCCACGGGC TGTCCTGGCT TCCCCTTGTG 
     2581 TGGCCACAGT AACCATCTTG GATGATGACC ATGCAGGAAT TTTCACTTTT GAATGTGATA 
     2641 CCATTCATGT CAGTGAAAGT ATTGGTGTTA TGGAAGTCAA GGTTTTGAGG ACATCGGGTG 
     2701 CCCGGGGCAC AGTCATCGTC CCTTTTAGGA CAGTAGAAGG AACAGCCAAG GGTGGTGGTG 
     2761 AGGACTTTGA AGATACTTAT GGGGAGCTGG AGTTTAAGAA TGATGAAACG GTGAAAACCA 
     2821 TAAGGGTTAA AATAGTAGAT GAGGAGGAGT ACGAAAGGCA AGAGAATTTC TTCATTGCCC 
     2881 TTGGTGAACC GAAATGGATG GAACGTGGAA TATCAGCGCT CCTGTTATCT CCAGAGGTGA 
     2941 CAGACAGGAA GCTGACTATG GAGGAAGAGG AGGCCAAGAG AATAGCAGAG ATGGGAAAGC 
     3001 CAGTATTGGG TGAACACCCT AAACTAGAGG TCATCATTGA AGAGTCCTAT GAGTTCAAGA 
     3061 GTACAGTGGA TAAACTGATC AAGAAGACAA ACCTGGCATT GGTTGTGGGG ACCCATTCCT 
     3121 GGAGGGACCA ATTCATGGAA GCCATCACTG TTAGTGCAGC AGGAGATGAG GAGGAAGATG 
     3181 AATCTGGAGA GGAGAGGCTG CCATCATGCT TTGACTATGT CATGCACTTC CTGACGGTCT 
     3241 TTTGGAAGGT GCTCTTTGCC TGTGTGCCCC CCACAGAGTA CTGCCATGGC TGGGCCTGCT 
     3301 TCGTGGTCTC CATCCTCATC ATCGGCATGC TCACCGCCAT CATCGGGGAC CTGGCCTCTC 
     3361 ACTTCGGCTG CACCATCGGG CTCAAGGATT CGGTCACAGC TGTTGTTTTT GTGGCATTCG 
     3421 GCACCTCTGT GCCAGATACA TTTGCCAGCA AAGCTGCTGC CCTGCAGGAT GTGTATGCAG 
     3481 ATGCTTCTAT TGGCAATGTC ACCGGCAGTA ATGCTGTCAA TGTCTTCCTG GGTATTGGTT 
     3541 TGGCCTGGTC CGTCGCCGCC ATCTACTGGG CCATGCAGGG ACAGGAGTTC CATGTGTCCG 
     3601 CTGGTACTCT GGCCTTCTCG GTCACTCTTT TCACCATCTT TGCATTTGTC TGCCTCAGTG 
     3661 TGCTCTTGTA CCGTCGGCGG CCCCACCTGG GCGGGGAGCT TGGAGGTCCT CGTGGCTGCA 
     3721 AGCTTGCCAC AACGTGGCTC TTTGTGAGCC TGTGGCTCCT CTACGTACTC TTTGCCACGC 
     3781 TGGAGGCCTA CTGCTACATC AAGGGGTTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3841 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3901 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3961 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4021 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4081 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4141 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4201 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4261 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4321 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4381 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4441 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4501 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4561 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4621 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4681 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4741 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4801 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4861 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4921 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4981 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5041 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5101 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5161 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5221 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5281 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5341 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5401 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5461 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5521 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5581 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5641 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5701 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5761 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5821 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5881 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5941 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6001 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6061 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6121 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6181 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6241 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6301 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6361 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6421 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6481 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6541 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6601 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6661 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6721 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6781 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6841 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6901 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6961 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7021 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7081 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7141 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7201 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7261 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7321 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7381 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7441 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7501 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7561 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7621 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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