LOCUS       RR207370                8496 bp    DNA     circular  SYN 
DEFINITION  Timeless (Myc-DDK-tagged ORF) - Rat timeless homolog (Drosophila) (Timeless), (10 ug)
ACCESSION   NM_031340
VERSION     NM_031340.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4653..4744 
                     /label="Myc-DDK"  
     misc_feature    4774..5359 
                     /label="PolyA signal"  
     misc_feature    4831..4850 
                     /label="Restriction Enzyme"  
     misc_feature    5635..6307 
                     /label="ColE1"  
     misc_feature    6737..7531 
                     /label="Kan/Neo"  
     misc_feature    7566..7868 
                     /label="SV40 ori"  
     misc_feature    7954..8431 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4635..4641 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4635 
                     /label="Timeless"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTTGTAC ATGATGAACT GTGAACTTCT AGCCACTTGT AGCGCCCTTG 
     1081 GGTACTTGGA AGGAGGCACT TACCACAAGG AGCCGGACTG CTTGGAGAGT GTGAAGGATT 
     1141 TGATCCGATA CCTGAGGCAT GAGGACGAGA CTCGAGATGT GCGGCAGCAG CTGGGAGCCG 
     1201 CGCAGATCCT GCAGAGTGAT CTCCTGCCAA TCCTCACCCA GCACCGCCAG GACAAGCCTC 
     1261 TCTTTGATGC TGTGATCAGG CTGATGGTAA ATTTGACTCA GCCAGCCTTG CTCTGTTTTG 
     1321 GCAGCGTGCC TAAGGACCCC ACTGTACGAC ACCATTTTCT GCAGGTTCTA ACCTACCTGC 
     1381 AGGCCTACAA AGAGGCCTTT GCCAGTGAAA AGGCATTTGG AGTCCTCAGC GAAACCTTGT 
     1441 ATGAGCTACT GCAGCTGGGC TGGGAGGATC GGCAAGAAGA AGACAACTTG CTGATTGAAC 
     1501 GGATCCTTCT GTTGGTCAGA AATATTCTCC ATGTCCCAGC CAACCTTGAG CAGGAGAAGA 
     1561 GGATCGATGA CGATGCCAGC ATTCATGACC GGCTTCTGTG GGCAATTCAC CTCAGTGGCA 
     1621 TGGACGACTT GCTCCTCTTC CTGTCCAGCT CATCCGCTGA GCAGCAGTGG AGCCTCCATG 
     1681 TGCTGGAGAT CATCTCCCTC ATGTTCCGAG ACCAGAAGCC TGAGCAGCTG GCAGGAGTAG 
     1741 GGCAGGGACG CTTGGCTCAG GAGCGAAGCA CAGATTTGGC AGAATTGGAA GTGCTGCGCC 
     1801 AACGGGAGGT GGCCGAGAAG AGAGCCCGAG CCCTGCAGCG AGGAAACAGG CACTCTCGAT 
     1861 TTGGGGGCTC CTATGTTGTC CAGGGGTTGA AATCTATTGG GGAGAGGGAT GTCGTCTTTC 
     1921 ACAAAGGCCT TCACAACCTC CAGAACTACA GCTCAGATCT AGGAAAGCAG CCCCGGAGGG 
     1981 TGCCTAAGCG TCGTCAGGCT GCCCAGGAGC TGTCTGTCCA TCGCCGCTCT GTCCTGAATG 
     2041 TGAGGCTCTT CCTCAGAGAC TTCTGCTCTG AGTTCCTGGA GAACTGTTAC AACCCACTCA 
     2101 TGGGTGCAGT CAAGGATCAC CTGCTTCGGG AGAAAGCTCA GCAACATGAT GAGACGTACT 
     2161 ACATGTGGGC GATGGCTTTC TTTATGGCCT TCAACCGAGC TGCTGCCTTC CGCCCTGGCT 
     2221 TTGTCTCTGA GACCCTCAGT ATCCGGACCT TTCACTTTGT GGAGCAGAAC CTCACCAACT 
     2281 ACTATGAGAT GATGCTGACA GACCGCAAGG AGGCCGCCTC CTGGGCACGC AGGATGCACC 
     2341 TGGCTCTGAA GGCCTATCAG GAGCTGCTGG CCACAGTGAA TGAGATGGAC ATGTGCCCAG 
     2401 ACGACGCTGT CAGGGAGAGC AGTCGCATCA TCAAAAACAA CATTTTCTAC ATGATGGAGT 
     2461 ACCGAGAACT ATTTCTGGCA CTCTTTCGAA AATTCGATGA GAGATACCAT CCACGTTCAT 
     2521 TCCTTTGTGA CCTGGTGGAG ACCACTCACC TCTTCCTCAA AATGTTGGAG CGCTTCTGTC 
     2581 GGAGCCGAGG GAACCTGATG GTGCAGAACA AAAGAAAGAA GAGAAAAAAG AAAAAGAAGG 
     2641 CCCAGGAGCA GGGTGTTGCT TTCTCACGTA GCCCTGAGGA GCTGCAGGCC ATGTGGTCAG 
     2701 CGCTGGCAGA GCGGCTGCTG CAGTGCGCCC AGGAACCTGA GCTCAGTGTG GACTCCATCA 
     2761 TTCCCTTTGA TGCGGCCTCA GAGGTGCCAG TGGAGGAGCA GCGGGTAGAA GCTATGGTGA 
     2821 GGATCCAAGA CTGCCTTGTG GCTGGCCAGG CCCCGCAAGC CTTGGCCCTC CTGCGGTCTG 
     2881 CCCGGGAAGT GTGGCCTGAA GGAAATGTGT TTGGCTCTCC AGTCATTTCC CCAGGGGAAG 
     2941 AAATGCAGTT GCTAAAACAA ATCCTCTCTG CAACCCTTCC CCGGCAGCAA GAGCCAGTAG 
     3001 AAGGAGATGC AGAGGAGGAA GATGAAGAGG AGGAGGAGGA GGAAGAGGAG GAGTTACAGG 
     3061 TGGTCCAGGT GTCAGAGAAG GAGTTTAAGT TTTTGGACTA CTTGAAACGC TTTGCATGCT 
     3121 CAACCATTGT GCGAGCCTAT GTGCTTCTCC TGCGGAGCTA CCGGCAGAAC AGCGCTCACA 
     3181 CCAACCACTG CATCGCCAAG ATGCTGCACC GGCTGGCCCA TGACCTGGGG ATGGAAGCCC 
     3241 TGCTTTTCCA GCTCTCCCTG TTCTGCCTCT TCAACCAGCT ACTCAGTGAC CCAGCCGCTG 
     3301 CAGCCTACAA AGAGCTAGTG ACTTTTGCCA AATACATCCT CGGCAAGTTC TTTGCGTTGG 
     3361 CTGCAGTGAA CCAGAAAGCA TTTGTGGAGC TGCTGTTCTG GAAGAACACC TCGGTGGTTC 
     3421 GGGAAATGAC CCAGGGATAT GGCTCCCTTG ACAGTGGGTC TTGCAGCCAC AGAGCCCCTG 
     3481 TGTGGAGCTC TGAGGAAGAG GCCCAGCTTC AAGAACTATA CCTCGCCCAC AAGGATGTAG 
     3541 AAGGTCAGGA TGTAGTGGAG ACCATCTTGG CACACCTGAA AGCTGTCCCT CGGACACGCA 
     3601 AGCAGGTCAT CCACCATCTG GTACGCATGG GCCTGGCCGA CAGTGTCAAG GACTTCCAGA 
     3661 GGAAAGGGAC CCAGATTGTC TTGTGGACAG AGGATCAGGA GCTGGAGTTA CAGCGGCTCT 
     3721 TTGAGGAGTT CCAGGATTCC GATGATGTTC TTGGTCATAT CATGAAGAAT ATCACAGCCA 
     3781 AACGTTCACG GGCTCGAGTA GTGGACAAAC TGTTGGCCCT GGGACTCGTG TCCGAGCGCA 
     3841 GGCAACTGCA CAAGAAACGG AGGAAGAAGC TGGCGCCCTC TTGCATGCAG AATGGGGAAG 
     3901 AGTCCCAGAG AGATGCTTGG CAGGAAGATC CAGAAGAAGA AGAGGAAAAA GAAGGCTTGC 
     3961 CAGAGAGTGA GGGTGAGGAG AACGAGGAAG ACTTGTTAGC AGGACAGGTC CAGGGCAGCT 
     4021 CATCTCTTTC TGCTGAAAAC CTCCGTCAGA GCCTTTGTCA GGACGGCCTC TCTGCTCCCC 
     4081 TCCTGTGGCT CCAGAGCTCC CTGATTCGCG CAGCAGATGA CCGAGAAGAG GATGGCTGCT 
     4141 CCCAGGCCAT CCCTCTGGTG CCTCTGACGG AAGAAAATGA GGAAGCAATG GAGAACAAAC 
     4201 AGTTTCAGCA TCTGCTACGT AAGCTAGGAA TCCGGGCCCC CTGCTCAGGA CAGGAAACCT 
     4261 TCTGGCGAAT TCCAGCCAAA CTGAGCTCCA CCCAGCTTCG GAGAGTGGCT GCTTCTCTGA 
     4321 GTGAGCGAGA AAACAAGGAG GAAAGAGAGG AGGAGCCAGA GCCGAATCCA GGAGTCCCTG 
     4381 GAGAGCAGAG TCCCAGTGAG GAGCACCAAG TGAGAGCCCC GAGAGCCCTT TTGTCAGCTC 
     4441 GTAAAAGGAA AGCAGGCCTG GTGTTTCCAA AAGAGGAGGC CACTGGGGAG GAAGAATGGA 
     4501 AATCGGTGCC TAAGAAGCAG CAACTGCTGG ACAGCGACGA AGAGGACACC GACGACGAGA 
     4561 GGGGAGGGCA AGCAGCAGTG TCGGGAACCC TGAGAATCCA CAAGGAGAAA CGGTTTCTGG 
     4621 TTGAGGATGA AGATGAGGAT TACACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4681 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4741 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4801 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4861 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4921 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4981 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5041 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5101 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5161 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5221 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5281 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5341 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5401 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5461 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5521 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5581 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5641 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5701 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5761 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5821 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5881 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5941 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6001 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6061 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6121 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6181 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6241 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6301 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6361 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6421 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6481 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6541 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6601 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6661 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6721 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6781 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6841 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6901 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6961 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7021 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7081 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7141 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7201 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7261 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7321 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7381 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7441 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7501 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7561 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7621 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7681 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7741 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7861 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7921 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7981 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8041 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8101 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8161 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8221 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8281 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8341 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8401 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8461 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//