LOCUS       RR207365                9021 bp    DNA     circular  SYN 
DEFINITION  Enam (Myc-DDK-tagged ORF) - Rat enamelin (Enam), (10 ug)
ACCESSION   NM_001106001
VERSION     NM_001106001.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5178..5269 
                     /label="Myc-DDK"  
     misc_feature    5299..5884 
                     /label="PolyA signal"  
     misc_feature    5356..5375 
                     /label="Restriction Enzyme"  
     misc_feature    6160..6832 
                     /label="ColE1"  
     misc_feature    7262..8056 
                     /label="Kan/Neo"  
     misc_feature    8091..8393 
                     /label="SV40 ori"  
     misc_feature    8479..8956 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5160..5166 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5160 
                     /label="Enam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGTCTTGCT CCTTGGCTCT CTGTGGCTTC AGCAGTTTGT AGTCATTTCT 
     1081 ACCATAGACT ATTAAGAACA TATTTCAAAG ACAGGTTAAC AAGGTTCAAG TTTACTTTTT 
     1141 ATATAGTACA GCTTCTAAGT GGCATTGGCT CTCATCCTAA TCTTAAGGCA CCGAAGATTA 
     1201 AGCTCGATTG TGAGGACCCT CTCATAAGGG CTTATGAAAG TTTTGGTGAA AACATGTTGC 
     1261 TGCTTCAGTG CAGAAATACA GCTTCTCCTC CAAAGCCATG TGACATGGTA AAGATGAGTC 
     1321 TCCTTGTTTT CCTGGGTCTG CTTGGGGTCT CGGCTGCCAT GCCAATGCCA ATGCCCCGAA 
     1381 TGCCTGGATT TAGCAGTAAA AGTGAAGAGA TGATGCGATA TAATCAATTC AATTTTATGA 
     1441 ATGCCCCACC AATGATGCAT ATGGGCCCAT GGCAAAACGG TTTGCCGATG CCTCCGCACA 
     1501 TGCCTCCGCA GTACCCTCCA TACCAGATGC CCATGTGGCC TCCGCCAGTC CCCAATGGAT 
     1561 GGCAGCAACC CCCAATGCCC AATTTCCCAA GCAAAACTGA ACAAACCCAG GAGACCGCCA 
     1621 AACCCAACCA GACCGATCCA CAAGAGCCAC AACCACAAAA GCAGCCTTTA AAGGAACCAC 
     1681 CAAATGAAGC AGCACGAGCC AAAGATGAAG CCCAGCCACC TCAGCCATTC CCACCATTTG 
     1741 GCAATGGGCT TTACCCCTAT CAACAACCAC CATGGCCAAT TCCACAGACA GGACCACCAA 
     1801 CAGGGTTCGG ACGACCCAAG TTCAGCAATG AAGAAGGAAA TCCTTATTAT GCATATTTTG 
     1861 GATATCACGG CTTTGGGGGC CGTCCTTATT ACTCAGAAGA GATGTTTGAA GATTATGAAA 
     1921 AACCCAAAGA AAAAGATCCT CCTAAACCTG AGGACCCGCC CCCAGATGAC CCACCCCCAG 
     1981 AGGCCTCTAC AAATTCAACA GTGCCTGATG CTAATGCCAC TCAATCAATT CCTGGAGGGG 
     2041 GTCAAGGTGG AAATGACTCT AGCCCAGTAG GAAACACAGG CCCTGGGCCT AATGCTGGGA 
     2101 ACAATCCTAC AGTTCTAAAT GGTGTCTTCC CTCTCCCTAA AGTTAATGTT TCAGGCCAGG 
     2161 GAGTACCGAA AAACCAAATT CCGTGGAGAC CAAGTCAGCC AAATATTTAT GAAAATTATC 
     2221 CTTATCCAAA TTATCCTTCA GAAAGACAAT GGCAAACCAC TGATACCCAG GGGCCTAAAC 
     2281 AGAACGGACC TGGTTACCAA AATCCACAAA TCCAAAGGGG TCCTCAGTGG AATTCCTTTG 
     2341 CTTGGGAAGG CAAACAAGCT ACTACTCATC CAGGAAATCC AACTTACCAC AAACCTTCCC 
     2401 CTCCTACCTC AAGAGTTAAT TATCCCAACT ATGCAGGAAA TCCAGTCAAT TTCGGAAGAA 
     2461 AACTGCCAGG GCCAAAAAAA CCCTTTATGG GAGCCAATCC AGCTTCAAAC AAACCCTTTG 
     2521 TGGGAGCCAA TCCAGCTTCA AACAAACCCT TTGTGGGAAC CAATCCAGCC TCAAACAAAC 
     2581 CCTTTGTGGG AGCCAATCCA GCCTCAAACA AACCCTTTGT GGGAGCCAAT CCAGCCTCAA 
     2641 ACAAACCCTT TGTGGGAGCC AATCCAGCCT CAAACAAACC CTTTGTGGGA GCCAACCCAG 
     2701 CCTCAAACAA ACCCTTTATG GGCACCAATC CTGCTGCAAA TAAACCATCT ATAGGAACCA 
     2761 ATGCAGCCTC AAACAAACCA TTTGTGGGAA CCAATGCAGG TACAAATAAA CCGTTTATGG 
     2821 GAATCAATGT TGCCTCAAAT AAACCCTTTA TGGGAACCAA TCCGGCCTCA AATAAACCCT 
     2881 TTGTGGGAAC CAATCCAGCC TCAAATAAAC CATTTGTGAG AAGCAATCAT GCCTCAAATA 
     2941 AACCATTTGT AGGAGCCAAT GCTGCTTCAA ATAAACCATT TGTGGGAACC AATGTGCCCT 
     3001 CAGTGGGTCC TAAACAGGTT ACTGTTAGCC ACAATATGAA AACCCAAAAT CCAAAGGAAA 
     3061 AGTCACTAGG TCAAAAAGAA AGAACAGTCA CTCCTACCAA AGATACAGGC AACCCCTGGA 
     3121 GAAGCTCTAA ACAATATGGA ATTAACAATC CAAACTATAA TTTGCCTCAC CCTGAGGGTA 
     3181 GCATGGTAGG CCCAAATTTT AATTCCTTTG ATCAACAAGA AAACACCTAC TTCTCAAGAG 
     3241 GAGATTCCAG AAAAGTAGCA AGTCCTAATA GACAAATCCA AAGTCAGAAT TTGCCCAAAG 
     3301 GAATTGCTTT AGAGCCAAGA AGAACCCCAT TTGAATCAGA AACTAAAAGA CCTGAATTAA 
     3361 AGCATAGTAC AAACATGCCT GTGTACCCTA AGAAAAACCC TTCTCCTACA AGAGGACATT 
     3421 TCCCTGCTGG AAGAAATAAC TGGAATCACC AAAAAACCCT TCCGCCCTTA AAAGAAGGCC 
     3481 ATGGGAGGAA AGAAGAAAAT TTACGTCATC CTCCCTATGG CTCTAGAGGA AATGTTTTTT 
     3541 ACCATGAATA TAACAATCCT TATCCTAATG AGAAATCACC GTACCTTAGA GGCAATACAT 
     3601 GGGATAAGAG GATCAGCACT CCCGGTACTA TGATGCAACC AGAAAATCCA CAGTATACCA 
     3661 TGTCTTCTCT AGACCAGAAG GAAACAGAGC AGTACAATGA AGAGGACCCA ATTGATCCAA 
     3721 ATGAAGATGA ATCTTTTCCA GGACAAAGTA GATGGGGTGA TGACGAGCTG AGCTTCAAAG 
     3781 GAAACCCAAC ACTTAGGCAG TATGAAGGTG AGCAATACAC CTCAACCCTG GCGAAGGAAT 
     3841 ACCTTCCCTA TTCCCTAAGT AATCCACCAA AGCCCAGTGA AGACTTTCCT TACAGTGAAT 
     3901 TCTATCCCTG GAACCCACAT GAAACTTTTC CAATCTATAA CCCAGGACCT ACTATAGCAC 
     3961 CACCTGTGGA CCCCAGAAGT TATTATGTTA ACAATGCAGT AGGACAAGAA GAAAGCACTC 
     4021 TCTTTCCTTC ATGGACCTCC TGGGACCACA GGAATCAAGC TGAGAGGCAG AAAGAAAGTG 
     4081 AGCCGTATTT CAACAGAAAT GTCTGGGATC AGTCAATGAG TTTACACAAA TCTAATATAC 
     4141 AAAACCCAAA CCATCCTTAT TCCACTACAT CCCCTGCTGG ATTTCCAAAA GATCCAACGT 
     4201 GGCTTGAAGG TGAGAATTTG AACTATGATT TACAAATTAC TAGTTTAAGT CAACCAGAGA 
     4261 GAGAACAGTT GGCTTTCCCA GACTTGAGGC CTCAAAGTTA CCCAACAGGC CAAAATGAAG 
     4321 ACCATTTATT TCACCAAAGT CAGAGAGGGT CTTGCTGCAT TGGTGGCTCC ACAGGACATA 
     4381 AAGAAAATGT GCTGGCTTTA CACGACTACA CTTCATCCTA TGGCCTTCCA CCAAGAAAGA 
     4441 CCCAAGAAAT CAGTCCTGTG CATACAGAAA GCAGTTATAC CAAATATGCA AAACCTAATG 
     4501 TTTCCCCAGC AAGCATCCTA CCTAATCAAA GAAATATCTC AGAGAACAAA CTGACTGCAG 
     4561 AAAGCCCAAA CCCAAGTCCA TTTGGAGATG ATGTGCCTAC TGTGAGAAAA AACACTCCAA 
     4621 ATTCTGGAAA GAATCAACTG GAGACAGGAA TTATAGCCCT TTCTGAAGCC AGCTCTTCTC 
     4681 AGCCAAAAAA CACAGCCTGT CTTAAAAGTG ACCTTGGAGG AGATCGGAGG GATGTTCTGA 
     4741 AACAGTTTTT CGAAGGCACC CAGCTCGGTG AAAGAACTGC TGGCCTGACC CCTGAGCAGC 
     4801 TCGTCATTGG TAGTCCTGAT AAAGGCTCTG GCCCAGATGG CACACACAGT GAAGTCCAAG 
     4861 GCAGTGAGGG TGAAATGCAG CAGCAGAGGC CACCCACCAT CCGGAAGTTG CCATGCTTTG 
     4921 GTTCCAAATT AGCAAAACTT CACTCTTCTA GCACTGGACC TCCAACTAAC AATGGAGGGC 
     4981 CAAGCCTACC TAATGGTGCT CTCTCCACAC CCACCGAAAG TCCTGACACA TTGGTTGGGT 
     5041 TAGCTACTAG GGAACAATTT AAAAGTATAA ATGTCGATCA ACTTAATGCA GATGGACACA 
     5101 CAACACTTGA ATCTTTTCAA GACACCAGTC AACAGGACCA AGCACACGGC TGTTTACTGC 
     5161 TTCAGGCTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5221 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5281 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5341 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5401 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5461 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5521 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5581 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5641 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5701 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5761 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5821 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5881 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5941 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6001 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6061 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6121 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6181 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6241 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6301 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6361 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6421 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6481 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6541 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6601 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6661 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6721 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6781 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6841 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6901 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6961 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7021 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7081 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7141 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7201 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7261 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7321 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7381 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7441 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7501 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7561 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7621 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7681 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7741 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7801 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7861 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7921 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7981 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8041 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8101 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8161 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8221 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8281 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8341 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8401 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8461 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8521 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8581 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8641 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8701 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8761 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8821 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8881 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8941 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9001 CAAAAATTTA ACGCGAATTT T
//