LOCUS       RR207317                8046 bp    DNA     circular  SYN 
DEFINITION  Usp25 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 25 (Usp25), (10 ug)
ACCESSION   NM_001107114
VERSION     NM_001107114.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4203..4294 
                     /label="Myc-DDK"  
     misc_feature    4324..4909 
                     /label="PolyA signal"  
     misc_feature    4381..4400 
                     /label="Restriction Enzyme"  
     misc_feature    5185..5857 
                     /label="ColE1"  
     misc_feature    6287..7081 
                     /label="Kan/Neo"  
     misc_feature    7116..7418 
                     /label="SV40 ori"  
     misc_feature    7504..7981 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4185..4191 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4185 
                     /label="Usp25"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCGTGGAG CAGAATGTGC TGCAGCAGAG CGCGGCGCAG AAGCACCAGC 
     1081 AGACATTTCT GAATCAGCTG AGAGAAATAA CTGGGATCAA TGACGCCCAG ATACTGCAGC 
     1141 AAGCCCTGAA GGATAGTAAT GGAAACTTGG AATTAGCAGT GGCCTTCCTC ACTGCAAAGA 
     1201 ATGCCAAGAC CCCCCCGCAG GAGGAGACGG CCTATTACCA GACAGCAGCA CTTCCGGGCA 
     1261 GCGACAGGTA CATCAGCGTG GGGAGCCAGG CAGATGCGAA TGTGATTGAT CTCACTGGAG 
     1321 ACGACAAAGA TGATCTTCAG AGAGCGATTG CTTTGAGTTT GGCGGAATCA AACAGGACGT 
     1381 TCAGGGAAAC TGGCATAACC GATGAGGAAC AAGCCATCAG CAGAGTTCTT GAAGCCAGCA 
     1441 TAGCAGAAAA TAAAGCATGT TTGAAGAGAA CACCTATAGA AGTTTGGAGG GATTCTCGAA 
     1501 ACCCTTATGA CCGGAAAAGA CAAGATAAGG CTCCGGTTGG GCTGAAGAAC GTTGGCAACA 
     1561 CCTGCTGGTT TAGCGCAGTT ATTCAGTCAT TATTCAATCT TTTGGAATTT AGAAGATTAG 
     1621 TTCTGAATTA CAAGCCTCCA TCAAATGCTC AAGATTTACC CCGAAACCAA AAGGAACATC 
     1681 GGAATTTGCC TTTTATGCGG GAATTGAGGT ATCTGTTTGC ACTTCTTGTT GGTACGAAGA 
     1741 GGAAATATGT TGATCCATCC AGAGCAGTTG AGATTCTAAA GGATGCCTTC AAATCAAATG 
     1801 ACTCACAGCA GCAAGATGTG AGTGAGTTTA CACACAAGTT ATTGGATTGG CTGGAAGATG 
     1861 CCTTCCAAAT GAAAGCTGAA GAGGAAACGG ATGAAGAGAA GCCAAAGAAC CCTATGGTAG 
     1921 AGTTGTTCTA CGGAAGATTC CTAGCCATGG GAGTGCTTGA AGGAAAAAAA TTTGAAAACA 
     1981 CTGAAATGTT TGGTCAGTAT CCGCTCCAGG TCAATGGGTT CAAAGACCTA CATGAGTGCC 
     2041 TGGAAGCTGC AATGATCGAA GGAGAGATCG AGTCCTTACA CTCGGATAAT TCAGGAAAAT 
     2101 CGGGCCAAGA GCATTGGTTC ACTGAATTGC CACCTGTATT AACATTTGAA CTGTCAAGAT 
     2161 TTGAATTTAA TCAGGCATTG GGAAGACCAG AAAAAATTCA CAATAAACTA GAATTTCCCC 
     2221 AAGTTTTATA CCTGGACAGG TATATGCACA GAAACAGAGA AATAACAAGA ATTAAACGTG 
     2281 AAGAGATCAA GAGACTTAAA GATTACCTCA CAGTATTACA ACAAAGATTA GAGAGATATT 
     2341 TAAGCTATGG CTCCGGTCCC AAAAGATTCC CACTGGTCGA TGTTCTACAG TATGCGTTGG 
     2401 AGTTCGCCTC CAGTAAGCCT GTCTGCACCT CTCCTGTGGA TGATATCGAT GCTACTTCCT 
     2461 CAACCAGTGG CTCCACATCA TCACAGTCGT TACCAAGCAC AACAGAACAG CAGGGAACCC 
     2521 CTGCTTCAGA CCTGCCCAGC TCGTCACCTG CATCAGGTGC TGCCCTGTCA CTGAGGTCAG 
     2581 TGATACACAA ACCCTTCACT CAGTCGCGGA TCCCTCCAGA CTTGCCCATG CATCCGGCAC 
     2641 CAAGGCACAT CACAGAGGAA GAACTGTCTG TGCTGGAGAG CTGTTTACAT CGCTGGAGGA 
     2701 CGGAAATAGA AAATGACACA CGAGACCTGC AGGAAAGCAT ATCTAGAATC CATCGAACAA 
     2761 TCGAACTGAT GTACTCGGAC AAATCCATGA TCCAGGTCCC TTACCGCCTC CATGCTGTCT 
     2821 TAGTTCATGA GGGCCAGGCT AATGCCGGCC ACTACTGGGC GTACATCTTT GACCATCGTG 
     2881 AGAGCAGGTG GATGAAGTAC AATGATATTG CTGTGACCAA GTCATCATGG GAAGAACTAG 
     2941 TGAGGGACTC TTTTGGTGGT TATAGAAATG CCAGTGCATA CTGCTTAATG TACATAAATG 
     3001 ACAAGGCACA GTTCTTAATA CAAGAGGAGT TCAATAAAGA GACTGGGCAG GCCCTTGTTG 
     3061 GAATGGAAAC ATTACCTCCA GACTTGAGGG ATTTTGTGGA GGAAGACAAC CAGCGCTTCG 
     3121 AGAAGGAGCT GGAAGACTGG GACACACAGC TCGCCCAACG AGCACTGCAA GAAAAGCTGC 
     3181 TGGCTGCTCA GAAGCTGCGG GAAGCAGAGT CTTCTGCGCC CACAGCACAA GCAGGAGGAC 
     3241 CAGACTATCT AGAGCAGCCA TCAAGGAGTG ACTTGTCAAA ACACTGGAAA GAAGAAACTC 
     3301 TCCGAGTGAT TGCCAAGGCA TCACATGATC ACGAAGATAA AGGGCCAGAG ACAGTTTTGC 
     3361 AGTCGGCAAT AAAATTAGAG TATTCAAGGC TGGTCAAGTT GGCCCAAGAA GATACCCCAC 
     3421 CAGAAACAGA TTATCGTTTA CATCATGTCC TGGTCTACTT TATCCAGAAC CAGGCACCAA 
     3481 AGAAAATCAT TGAGAAAACA TTACTCGAAC AATTTGGAGA TAGAAATTTG AGTTTTGATG 
     3541 AAAGGTGTCA CAACATAATG AAAGTTGCCC AAGCCAAACT GGAAATGATC AAACCTGAAG 
     3601 AAGTGAACTT GGAGGAATAT GAGGAGTGGC ACGCAGATTA TAAGAAATTC AGAGAAACAA 
     3661 CTATGTATCT CATAATTGGA CTAGAAAACT TTCAGAGAGA AAGTTATATA GACTCCTTGC 
     3721 TGTTCCTTAT CTGTGCTTAT CAGAATAACA AAGAGCTCTT GTCCAAGGGT CCATACAGAG 
     3781 GGCATGATGG AGACTTGATA TCACATTACA GAAGAGAGTG TTTACTGAAA TTAAATGAGC 
     3841 AAGCGGCAGA GCTGTTCGAG TCTGGAGAGG ACCGAGAAGT GAGCAATGGC TTAATTATTA 
     3901 TGAATGAGTT TATCGTTCCC TTTTTGCCCT TGTTGCTGGT GGATGAAACG GAAGAGAGAG 
     3961 ATATCCTTGC TGTGGAGGAC ATGAGGAACC GATGGTGTTC ATACCTGGGG CAAGACATGG 
     4021 AACCCAACCT CCAAGAAAAG CTGACGGATT TTCTGCCAAA ACTGCTTGAT TGTTCTACAG 
     4081 AGATTAAAGG TTTCCACGAG CCGCCAAAGT TACCTTCCTA TTCTGCGCAT GAACTCTGCG 
     4141 AGCGATTCGC CCGAATCATG TTGTCCCTCA GTCGCACTCC TGCTGATGGA AGAACGCGTA 
     4201 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4261 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4321 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4381 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4441 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4501 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4561 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4621 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4681 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4741 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4801 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4861 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4921 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4981 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5041 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5101 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5161 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5221 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5281 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5341 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5401 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5461 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5521 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5581 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5641 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5701 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5761 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5821 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5881 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5941 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6001 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6061 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6121 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6181 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6241 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6301 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6361 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6421 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6481 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6541 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6601 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6661 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6721 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6781 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6841 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6901 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6961 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7021 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7081 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7141 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7201 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7261 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7321 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7381 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7441 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7501 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7561 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7621 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7681 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7741 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7801 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7861 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7921 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7981 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8041 AATTTT
//