LOCUS       RR207193                6729 bp    DNA     circular  SYN 
DEFINITION  Sphk2 (Myc-DDK-tagged ORF) - Rat sphingosine kinase 2 (Sphk2), (10 ug)
ACCESSION   NM_001012066
VERSION     NM_001012066.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2886..2977 
                     /label="Myc-DDK"  
     misc_feature    3007..3592 
                     /label="PolyA signal"  
     misc_feature    3064..3083 
                     /label="Restriction Enzyme"  
     misc_feature    3868..4540 
                     /label="ColE1"  
     misc_feature    4970..5764 
                     /label="Kan/Neo"  
     misc_feature    5799..6101 
                     /label="SV40 ori"  
     misc_feature    6187..6664 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2868..2874 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2868 
                     /label="Sphk2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCACCA CCACTACTGC CAGTGGCTGC CAGCACTCCG ATCCTGCACG 
     1081 GCGAGTTTGG TTCCTACCCA GCCCATGGCC CCAGGTTTGC CCTCACCCTC ACGACACAAG 
     1141 CCCTACACAT ACAGCGACTG CGCCCAAAGC CAGAAGCCCG GCCCCGAGAT GGTCTAGTCC 
     1201 CTCTGGACGA GGTCTCAGGC TGTGGCACTC TGCAGAGCCG TAGCCCCGAA GACACTGCAG 
     1261 CCTACTTCTG CATCTACACC TACCCACGTG GCCGGCGTGG GGGCCGGCGC AGAGCTACTC 
     1321 GTACCTTCCG GGCCGACGGG GCCGCCACTT ATGTGGAGAA TCGTGCAGAG GCCCAGCGTT 
     1381 GGGCAACTGC TCTCATGTGT CTCCTCCGAG GAGTGCCTCT GTCTGGGGAC CAGGAAATCA 
     1441 CCCCTGAATT GCTGCCCCGG AAGCCCAGGC TGCTCCTATT GGTCAATCCT TTTGGGGGGC 
     1501 GGGGCCTGGC CTGGCAGCGC TGTATGGACC ACGTGGTGCC CATGATCTCT GAAGCTGGGC 
     1561 TGTCCTTCAA CCTCATACAG ACAGAACGAC AAAACCATGC ACGTGAGCTG GTGCAGGGGT 
     1621 TGAGCCTGAG TGAGTGGGAA GGCATTGTCA CTGTGTCCGG AGATGGGCTG CTTTATGAGG 
     1681 TGCTGAATGG GCTCCTCGAC CGCCCAGATT GGGAAGACGC TGTGCGGATG CCCATTGGTG 
     1741 TCCTCCCCTG TGGTTCGGGC AATGCACTAG CTGGGGCAGT TAACCATCAT GGCGGGTTTG 
     1801 AGCAAACTGT TGGTGTTGAC CTGTTGCTCA ACTGCTCGCT TCTTCTCTGC CGGGGTGGCA 
     1861 GCCATCCTCT GGACCTGCTC TCTGTGACGC TAGCCTCAGG ATCCCGCTGT TTTTCCTTCC 
     1921 TGTCAGTTGC CTGGGGATTC TTGTCAGATG TGGACATTCA CAGCGAGCGC TTCAGAGCCC 
     1981 TGGGCAGTGC TCGATTCACA CTGGGTGCAG TGCTAGGCCT GGCCACATTG CATACCTACC 
     2041 GTGGACGTCT CTCCTACCTC CCTGCTACCA CAGAACCAGC CTTGCCCATC CCAGGCCATG 
     2101 GTCTGCCAAG AGCCAAGTCA GAACTAGCCT TGGCCCCAGC CCCAGCCCCA GCCGCCACCC 
     2161 ACTCACCTCT TCATCGATCT GTGTCTGACC TGCCCCTGCC TCTTCCCCAG CCTGCCCTGG 
     2221 CCTCCCCTGG CTCCCCTGAG CCCCTGCCTG ACCTGTCCCT CAATGGTGGT GGTCCAGAGC 
     2281 TGACTGGAGA CTGGGGGGGA GCTGGGGATG CACCTTTGTC CCCAGACCCA TTGCTGCCTT 
     2341 CATCCCCCAA CGCTTTCAAA ACAGCTCAGC TTTCACCCAT CGCCGAAGGG CCCTCAGAAA 
     2401 TGCCAGCATC AGGGCTGCTG CCTCCCATCC ACAATGCTCC AGAAGCCTCT ACCTGGGGCC 
     2461 CAGCGGACCA CCTGCTCCCT CCCCTGGGCT CTCCACTGCC CCAAGACTGG GTGACAATAG 
     2521 AGGGGGAGTT TGTACTCATG TTGGCCATCT TGCCAAGCCA CCTCTGCGCA GACCTGATGG 
     2581 CAGCCCCACA CGCGCGTTTT GACGATGGTG TGGTGCACCT GTGCTGGGTG CGGAGCGGCA 
     2641 TCTCACGGGC CGCACTTCTA CGAATTTTGC TGGCCATGGA ACATGGAAAC CACTTCAGCC 
     2701 TGGGCTGCCC ACATCTGGGC TATGCTGCAG CACGTGCCTT CCGCCTTGAG CCACTCACGC 
     2761 CTCGTGGGGT GCTCACTGTA GATGGGGAAT TAGTAGAGTA TGGGCCAATA CAGGCACAGG 
     2821 TGCATCCAAG TCTCGCCACA CTGCTCACTG GGCCTGCAGG TCAAAAGCCT CGAGCCACGC 
     2881 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     2941 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3001 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3061 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3121 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3181 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3241 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3301 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3361 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3421 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3481 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     3541 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     3601 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     3661 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     3721 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     3781 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     3841 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     3901 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     3961 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4021 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4081 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4141 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4201 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4261 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4321 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4381 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4441 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4501 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     4561 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     4621 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     4681 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     4741 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     4801 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     4861 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     4921 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     4981 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5041 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5101 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5161 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5221 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5281 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5341 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5401 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5461 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     5521 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     5581 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     5641 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     5701 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     5761 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     5821 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     5881 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     5941 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6001 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6061 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6121 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6181 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6241 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6301 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6361 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6421 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6481 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     6541 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     6601 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     6661 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     6721 GCGAATTTT
//