LOCUS       RR207109                8613 bp    DNA     circular  SYN 
DEFINITION  Skiv2l (Myc-DDK-tagged ORF) - Rat superkiller viralicidic activity 2-like (S. cerevisiae ) (Skiv2l), (10 ug)
ACCESSION   NM_213559
VERSION     NM_213559.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4768..4859 
                     /label="Myc-DDK"  
     misc_feature    4889..5474 
                     /label="PolyA signal"  
     misc_feature    4946..4965 
                     /label="Restriction Enzyme"  
     misc_feature    5750..6422 
                     /label="ColE1"  
     misc_feature    6852..7646 
                     /label="Kan/Neo"  
     misc_feature    7681..7983 
                     /label="SV40 ori"  
     misc_feature    8069..8546 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4743..4750 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4743 
                     /label="Skiv2l"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGAGACC GAGCGACTCG TGCTGCCTCC CCCAGACCCC CTGAACCTGC 
     1081 CCCTTCGAGC TCTGGAAGTG GGGTGCACTG GCCGTTGGGA GCTGCTAAAC GTGCCAGGGC 
     1141 CTCCAGAGAG TACTCTTCCC CATGGCCTCC CTCCCTGTGC CCCAGATCTG TGCCAGGAAG 
     1201 CAGAGCAGTT GTTTCTGTCG TCTCCGGCCT GGCTTCCTCT ACACGGTGTG GAGCACTCAG 
     1261 CTCGAAAATG GCAGAGGAAG ACAGATCCCT GGTCGCTTCT GGCTGCTCTG GGGACTCCAG 
     1321 TCCCATCCGA CCTTCAGGCC CAGAGACACC CAACCACAGG CCATATACTG GGCTATAAGG 
     1381 AGGTCCTGCT GGAGAACACG AACCTGTCGG CCACCACCTC CTTGTCCCTT CGCCGGCCTC 
     1441 CAGGGCCAGC CTCCCAGTCG CTATGGGGCA ATCCGACACA GTACCCTTTC TGGCCGGGTG 
     1501 GGATGGACGA GCCCAGCATA ACTGATCTGA GTACTCGGGA GGAAGCCGAG GAGGAGATAG 
     1561 ACTTTGAGAA AGATCTTCTC ACCGTTCCAC CCGGCTTCAA GAAAGGGGTG GATTTTGCCC 
     1621 CCAAAGCTCC TGTGCCAGGT TTGCTCAGCC TCAGCCGTCT GCTGGAGCCT CTGGATCTGA 
     1681 GTGGAGGTGA TGAGGGTGAG GGAGAGGCAG CAGGAGGTCC TAGAGGGGAT GCGGCCTCAG 
     1741 CCTCTCCCTC CAGCACTCCC CTGATCCGGG CAAGCAGCTT GGAGGACCTA GTGTTGAAGG 
     1801 AAGCTTCCAC AGTCGTATCC ACCCTGGAGC CCCTCAAACC TCCACCTCAG GAACAGTGGG 
     1861 CTGTGCCTGT GGACGTCACC TCCCCCGTGG GTGATTTTTA CCGTCTCATC CCTCAGCCGG 
     1921 CCTTCCAGTG GGCGTTCGAG CCAGATGTGT TTCAGAAGCA GGCCATCCTG CACTTGGAGC 
     1981 AGCACGACTC TGTCTTTGTT GCAGCTCACA CATCCGCTGG GAAGACGGTG GTGGCCGAGT 
     2041 ATGCCATTGC CCTGGCCCAG AAACACATGA CCCGTACCAT CTACACTTCT CCTATCAAAG 
     2101 CCCTGAGCAA CCAGAAGTTT CGGGACTTCC GAAACACATT CGGGGATGTG GGACTGCTCA 
     2161 CGGGGGATGT GCAGCTGCAC CCAGAGGCCT CCTGCCTCAT CATGACCACG GAGATCCTTC 
     2221 GCTCCATGCT GTACAGTGGC TCAGACGTCA TCCGAGACCT GGAGTGGGTC ATTTTTGATG 
     2281 AAGTCCACTA TATCAATGAT GCTGAGCGTG GGGTCGTGTG GGAGGAGGTG CTCATTATGC 
     2341 TCCCTGAGCA CGTCTCCATC ATCCTGCTGA GTGCCACCGT CCCCAACGCC CTGGAGTTTG 
     2401 CTGACTGGAT CGGGAGGCTG AAGCGGCGGC AGATCTATGT GATCAGCACG GTGGCCCGTC 
     2461 CCGTCCCCTT GGAGCACTAT CTCTTCACAG GAAACAGCCC CAAGACACAG GGCGAACTCT 
     2521 TCCTTCTGCT GGACTCCCGA GGGGCCTTCC ACACCAAGGG GTACTACGCT GCCGTGGAGG 
     2581 CCAAGAAAGA GAGGATGAGC AAACACGCCC AGACCTTCGG GGCCAAGCAG CCCACACACC 
     2641 AGGGTGGGCC TGCGCAGGAC CGTGGGGTGT ACCTGGCCTT GCTGGCTTCC CTCCGGACCC 
     2701 GGGCTCAGTT GCCTGTGGTA GTGTTCACCT TCTCCCGAGG CCGCTGTGAT GAACAGGCTT 
     2761 CAGGTCTCAC CTCCCTAGAC CTCACAACAA GCTCAGAGAA GAGTGAGATC CACCTCTTCC 
     2821 TGCAGCGCTG TCTTGCCCGG CTCCGAGGCT CTGACCGACA GCTCCCCCAG GTCCTGCACA 
     2881 TGTCGGAGCT CCTCCGCCGA GGCCTGGGTG TGCACCACAG CGGCATCCTG CCCATCCTTA 
     2941 AGGAGATTGT GGAGATGCTT TTCAGCCGGG GCCTAGTCAA GGTCCTGTTT GCCACCGAGA 
     3001 CTTTTGCCAT GGGGGTAAAC ATGCCAGCCA GGACAGTGGT GTTTGACTCC ATGCGCAAAC 
     3061 ATGATGGCTC CACCTTCCGG GACCTGCTGC CTGGAGAGTA CGTGCAGATG GCAGGCCGGG 
     3121 CGGGCCGGAG GGGCCTGGAC CCCACGGGCA CCGTCATCCT TCTCTGTAAG GGCCGAGTGC 
     3181 CTGAGATGGC AGATCTACAC CGCATGATGA TGGGGAAACC ATCACAGCTT CAGTCCCAGT 
     3241 TCCGCCTCAC ATATACCATG ATCCTCAACC TGCTGCGGGT GGACGCCCTC AGAGTGGAAG 
     3301 ACATGATGAA GAGGAGCTTC TCAGAGTTCC CCTCCCGCAA GGACAGCAAG GCCCATGAGC 
     3361 AGGCTCTGGC TGACCTCACC AAGAGGCTGG GGGCTTTGGA GGAGCCTGAT GTGACTGGTC 
     3421 AGTTGGCTGA CCTGCCTGAG TATTACAGCT GGGCGGAGGA GCTGACAGAG ACCCGGAACA 
     3481 TGATCCAGCG ACGCATCATG GAGTCTGTGA ACGGGCTGAA ATCTCTGTCA GTGGGAAGGG 
     3541 TGGTGGTTGT GAAGAATGAA GAGCATCACA ATGCGCTGGG TGTGATCCTG CAGGTCTCCT 
     3601 CAAACTCCAC TAGCCGAGTA TTTACAACCT TGGTCTTGTG TGATAAACCT GCTGTGTCTG 
     3661 AGAACCCAAG GGACAAGGGG CCAGCCACTC CAGATGTGCC CCACCCAGAT GACCTTGTAG 
     3721 GCTTCAAGTT GTTCCTGCCT GAAGGGCCCT GCGAGCACAC TGTGGCCAAG CTCCAGCCAG 
     3781 GGGATGTGGC TGCCATCTCT ACCAAGGTGC TCCGGGTGAA TGGGGAGAAG ATTTCTGAGG 
     3841 ACTTCAGCAA GAGGCAGCAA CCGAAATTCA GGAAGGACCC TCCCCTCGCG GCCGTGACCA 
     3901 CTGCTGTTCA GGAGCTGCTG CGTCTGGCCC AGGCCTATCC AGCAGGACCC CCAACCCTCG 
     3961 ACCCTATCAA CGACCTGCAA CTGAAGGATG TGGCAGTGGT AGAAGGTGGG CTCCGGGCCC 
     4021 GGAAGCTGGA GGAGCTGATC CGAGGCGCTC AGTGTGTACA CAGCCCCCGG TTTCCTGCCC 
     4081 AGTATGTGAA GCTACAGGAG CGAATGCAGA TTCAGAAGGA AATGGAGAGG CTGCGCTTCC 
     4141 TCCTGTCTGA CCAGTCGCTG TTGCTGCTCC CCGAGTACCA CCAGCGTGTA GAGGTGCTTC 
     4201 GGACCCTGGG CTATGTGGAT GAGGCTGGCA CAGTGAAGCT GGCGGGCCGG GTGGCCTGTG 
     4261 CCATGAGCAG CCATGAGCTG CTCCTCACTG AACTCATGTT TGACAATGCA CTGAGTGCTC 
     4321 TGCGACCAGA GGAAATAGCA GCTCTGCTCT CTGGTCTGGT GTGCCAGAGC CCCGGAGACC 
     4381 CTGGGGATCA GCTGCCAAGC ACCCTCAAAC AGGGGGTAGA ACGCGTCAAA GCTGTGGCCA 
     4441 AGCGGATTGG TGAGGTTCAG GTGGCCTGCG GCCTGAACCA GACGGTGGAG GAATTTGTGG 
     4501 GGGAGCTGAA TTTTGGGCTG GTGGAAGTTG TTTATGAGTG GGCTCGGGGT ATGCCCTTCT 
     4561 CCGAGTTGGC AGGGCTCTCG GGGACCCCCG AAGGCTTGGT TGTCCGCTGT ATCCAGCGCC 
     4621 TGGCTGAGAT GTGCCGCTCA CTTCGAGGGG CGGCCCGTTT GGTAGGAGAG CCTGTGTTAG 
     4681 GGGCCAAGAT GGAGACGGCA GCCACCTTGC TCCGGAGGGA CATCGTCTTC GCAGCCAGCC 
     4741 TCTATACCCA GAGCGGACCG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4801 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4861 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4921 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4981 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5041 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5101 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5161 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5221 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5281 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5341 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5401 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5461 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5521 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5581 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5641 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5701 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5761 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5821 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5881 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5941 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6001 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6061 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6121 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6181 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6241 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6301 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6361 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6421 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6481 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6541 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6601 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6661 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6721 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6781 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6841 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6901 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6961 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7021 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7081 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7141 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7201 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7261 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7321 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7381 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7441 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7501 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7561 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7621 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7681 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7741 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7801 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7861 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7981 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8041 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8101 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8161 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8221 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8281 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8341 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8401 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8461 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8521 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8581 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//