LOCUS       RR207102                8532 bp    DNA     circular  SYN 
DEFINITION  Evc2 (Myc-DDK-tagged ORF) - Rat Ellis van Creveld syndrome 2 homolog (human) (Evc2), (10 ug)
ACCESSION   NM_001106012
VERSION     NM_001106012.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4689..4780 
                     /label="Myc-DDK"  
     misc_feature    4810..5395 
                     /label="PolyA signal"  
     misc_feature    4867..4886 
                     /label="Restriction Enzyme"  
     misc_feature    5671..6343 
                     /label="ColE1"  
     misc_feature    6773..7567 
                     /label="Kan/Neo"  
     misc_feature    7602..7904 
                     /label="SV40 ori"  
     misc_feature    7990..8467 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4671..4677 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4671 
                     /label="Evc2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGCGACC GGTCTCACGG GCACTGGGGG ACAAGCCACG TGGGTGCTGG 
     1081 CTGGAAATAT CCTGGCCGCG GCCTTGGTAC TGGGTGTGGG TCCGTGTGCG CTTCCACCCT 
     1141 CCTTCCCTGC CCTGGGCCCA GGGTCGCCAT CCCCACCCGA CCCTGTAGGA CCCCGGGCAA 
     1201 GCTCACAGCA CAGCGATATT TCAAGAGATG CCCAAGGTCC CTCAGAGAAC GGAGTGATAT 
     1261 TTCAAAAATG CTCGCTGGTG TCTGGGCAGA GCGAATCGCA GACCATGCAT GTGCAGCTGT 
     1321 CAGTGAACAA CACCAGGACG CCCATGTCAG CCAACCTTTC GGACCTGCTT GTGCTGGATG 
     1381 ACATCACTGG CCTCACTGTG AAGGAGTCAC CTGGAAATGT GACTCAGGAA GGAATCCAGA 
     1441 CTTTTAGAAA GCGGTTTCTG CAAGTGGGAG AGTGCTACTC CGTCAGCTAC ATGGCCTCGC 
     1501 TGGACCCCAC AGCCCTTGGG CCTGGAGAGA GCCTGGATCT TCCCGCCCAG CTCATCTTTC 
     1561 AGAGACCCTC ACAGAACAGA ACTCAGCTAA GAGCCCGCTT TACCATCACC GTGGAAGAAA 
     1621 AGATAATGGT TCTGCCTAAC CATGGTCTCC ACACAGCAGG CTTCATCGCA GCCTTCCTCA 
     1681 TCTCCTTCCT CCTGACCTTG GCAGCCCTGT TCTTCCTGGC TCGGGGTCGG TGTCTGCAGG 
     1741 GAAGCATGCT GTTGAGATGC CGGGTTCAGC ATCCTGAGAA CAAGCTGGAG CCCTCACCCT 
     1801 TCACCTCTGC TAATGGTGTG AGCCAGGACC TTTCCCTCAA TGACCAACTG GTTGCCATCC 
     1861 TGACTTCTGA GGAGCCTGGG AGCATGCTTC AAGCCCTAGA AGAGTTGGAG ATCGCAACCC 
     1921 TGAATCAGGC AGATGCGGAT CTGGAGGCTT GTCGAAACCA GATTAGTAAG GACATCATTG 
     1981 CCCTTCTGAT GAAGAATCTG ATCAGTGGTG GTCACCTCTC TCTGCAAACA GAAAGGAAGA 
     2041 TGGCTGCTGC TTTTAAAAAG CAGTTTCTGC TGCTGGAAAA CGAAATCCAG GAGGAGTATG 
     2101 AGCGGAAGAT GTTGGCACTC ACGGCAGAGT GTGACCTAGA AGTGAGGAAG AAGACAGAAA 
     2161 ACCAGTACCA GAGGGAGATG GTGGCCATGG AGGAAGCAGA AGAGGTGTTA AAGCGAGTTA 
     2221 GTGAGAGGTC TGCTGCAGAG TGCAGCAGCC TTTTGCGGAC CCTCCATGGG CTGGAGCAGG 
     2281 AGGACCTGCA GAGGTCCCTC ACTCTGCAGC AAGCAGAGGA CTTTGCCCAA GCACACAGGC 
     2341 AGCTGGCTGT TTTCCAGAGA AACGAATTAC ACAGCATCGT CTATACCCAG ATACAGAGTG 
     2401 CTGTTGCCAA AGGAGAACTG AGGCCCGAGG TGGCTAAAAT GCTGCTGCAA GACTATTCTA 
     2461 AAACACAGGA GAGTATAGAA GAGCTGATGG ACTTCTTCCA GGCCACCAAG CGATACCATC 
     2521 TCAGCAAGAG GTTTGGCCAC AGAGAGTACC TGGTCCAGAG ACTGCAGGCC ATGGAGACCC 
     2581 GCGTGCAAGG GCTGCTGAGC ACTGCAGCTA CCCAGCTAAC AAGTCTCATT CATAAACATG 
     2641 AGAGGGCTGG TTACTTGGAT GAAGATCAGA TGGAAATGCT ATTGGAGCGT GCACAGACAG 
     2701 AAACCTTTTC TATAAAACAG AAGCTGGACA ATGACTTAAA GCAAGAAAAG AAAAGGCTCC 
     2761 ACCAAAAATT AATAACCAAG AGACGGCGAG AACTTCTGCA AAAGCACAAG GAGCAGCAGA 
     2821 AAGAGCAGGT GTCCCTTGGC GAGGCCTCCA GCACTGCGGA GGATGCCATT CAGTACCTGC 
     2881 AGCAGTGGAG GAGCGTGATG GCCGAGCACA CCACTGCCCT GGAGGAGCTG CAGGAGCGGC 
     2941 TGGACCAGGC TGCCCTGGAT GACCTCCGGG TCCTGACTGT GTCACTGTCT GAGAAAGCCA 
     3001 CAGAGGAGCT GAGGCGCCTG CAGAGCACGG CCATGACACA GGAGCTGCTG AAGCGCAGTG 
     3061 CTCCCTGGCT CTTCCTGCAG CAGATCCTAG AGGAGCATAG CCGGGAGTCT GCGGCCCGTA 
     3121 CCGCGCAGCT GGAGGCAGAG GAGAGAGAGC GTGGCCAGGA GCTGGTGCAG GGCGTGAGAC 
     3181 AGAGGCTACA ACAGGATGCA CCGGAGGCTT GCACAGAAGA GCAGGCAGAG CTGAGGCATT 
     3241 GGGAGCACTT GGTATTCACG AAGCTCTGCT CTGCGGCCAT CTCACTGTCT GAAGAAGACC 
     3301 TGCTCCGGGT GAGGCAGGAG GCACAGGGCT GCTTCACCCA GCTGGACCGG AGCTTGGCCC 
     3361 TCCCCAGAGT CCGTGCACGG GTGCTGCAGC AGCAGATGCA GATGACCTGG CGGGAAGCAG 
     3421 AGTTCCGGAA ACTGGACCAG GCTCTGGCTG TGCCTGAGCT GCAGTCCAAG TCAAGAAAGC 
     3481 TGCGTTCCAA GGGCAGAGGC AAGGCGGACC TTCTGAAGAA GAACCTGGAG GATAAGATCC 
     3541 GCCTGTTCGA GGAGCGGGCC CCTGAAGACC TGGCTGACCA GGTACGAGGG GAATTGCTCC 
     3601 AGGAGAGGGT CCAGAGGCTG GAGGCCCAGG AGGCACACTT TGCCGAGTCA CTTGTGGCTC 
     3661 TGCAGTTCCA GAAGGTGGCC CGGGCTGCTG AGACCCTCTC CGTCTACACC GCCCTGCTTA 
     3721 GCATCCAAGA CCTGCTGCTG GGGGAGCTGA GTGAGTCGGA GACACTGACC AAGTCAGCCT 
     3781 GCGTGCAGAT CCTGGAGTCC CACAGGCCGG AGCTGCTGGA GCTGGAGAGG ATGCTGGAGG 
     3841 ACCGGCTGGT GCAGCAGGAA GAGGCTGAGC AGCGGCGGGT CCTGGAGAGC AGGCAGCGGT 
     3901 GGGCGGCTGA CGGACCTGCG CTGGGCGAGC CTGAGGAGAT AGATCCCGAG AGGCAGGTCT 
     3961 CTGCCGTCCT GCGGCAGGCT CTGAACAAGG GCCAGAAGCT ACTGGAACAG TATCAGCAGA 
     4021 GGGTGAGAGA GGAGCAGCAG AACGGTGCAG TGCTGGAAGA TTCTCTGGAA AGCATGGAGA 
     4081 CCGACACCAT GGCCAGCCTC TGCAGCCAGG GACTGAGGCT GGTGTCCTAC CTCTCCAGGA 
     4141 TGACAATGGT GCCGGGGGGC ACCCTACTGA GACTCCTGAG TGTGGTGCTT CCCGCAGCTT 
     4201 CACAGCCTCA GCTACTGGCC TTGCTGGATG CAGTCAGCGA GAAGCACTCA GACCACACAG 
     4261 CAGAGAATGA GAGTGGTGGT GAGCAGGCAC AGGCGGAGCA GGGCAAGAGG AGAAAACACC 
     4321 AGAGCTGGTG GAAAGCTTTG GACAGCAGGT TCAGAGCAGA TCTGGTGAGT CAAGGACTGG 
     4381 AGAGGATGCT CTGGGCCCGC CGGAAGAAGG AGAGCATATT GAAGAAGAGA TATGTTCCTG 
     4441 TCCAGGAGAG GGTGGTGTTC CCCGGGAAAG GAAGCTGGCC GCACCTGTCC CTAGAGCCCA 
     4501 TCGGAGAACT GGCGCCCATC CCCATCACAG GGCTAGACAC TATGGACATA TTAAACACAG 
     4561 GAGAAAAGAT CTTTGTATTC AGAAGCCCAA AGGAGCCTGA GATCTCTTTG CGTGTTCCTC 
     4621 CCAGAAGGAA GAAGAATTTC CTGAATGCCA AGGCAGCCAG CAGGGCCTTG GGCTTGGACA 
     4681 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4741 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4801 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4861 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4921 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4981 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5041 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5101 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5161 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5221 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5281 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5341 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5401 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5461 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5521 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5581 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5641 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5701 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5761 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5821 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5881 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5941 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6001 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6061 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6121 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6181 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6241 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6301 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6361 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6421 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6481 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6541 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6601 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6661 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6721 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6781 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6841 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6901 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6961 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7021 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7081 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7141 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7201 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7261 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7321 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7381 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7441 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7501 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7561 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7621 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7681 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7741 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7801 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7861 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7921 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7981 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8041 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8101 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8161 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8221 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8281 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8341 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8401 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8461 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8521 AACGCGAATT TT
//