LOCUS       RR207055                6969 bp    DNA     circular  SYN 
DEFINITION  Sestd1 (Myc-DDK-tagged ORF) - Rat SEC14 and spectrin domains 1 (Sestd1), (10 ug)
ACCESSION   NM_001134514
VERSION     NM_001134514.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3126..3217 
                     /label="Myc-DDK"  
     misc_feature    3247..3832 
                     /label="PolyA signal"  
     misc_feature    3304..3323 
                     /label="Restriction Enzyme"  
     misc_feature    4108..4780 
                     /label="ColE1"  
     misc_feature    5210..6004 
                     /label="Kan/Neo"  
     misc_feature    6039..6341 
                     /label="SV40 ori"  
     misc_feature    6427..6904 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3108..3114 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3108 
                     /label="Sestd1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCCTCG GTGATATTGC CCATCCTGAA GAAGAAGCTG GCCTTCCTTT 
     1081 CAGGAGGAAA GGACAGACGC AGTGGACTCA TCTTGACAAT TCCATTATGC CTTGAACAGA 
     1141 CAAATATGGA TGAGCTGAGT GTCACTCTGG ACTATCTACT CAGCATTCCA AGTGAGAAAT 
     1201 GTAAGGCCAG AGGATTCACT GTGATCGTGG ACGGCAGGAA GTCTCAATGG AACGTGGTGA 
     1261 AGACAGTGGT CCTAATGCTG CAGAACGTTG TTCCAGCTGA GGTATCCCTT GTTTGTGTAG 
     1321 TGAAGCCAGA TGAATTCTGG GATAAGAAAG TAACACATTT TTGTTTTTGG AAAGAAAAGG 
     1381 ATAGACTTGG CTTTGAGGTT ATTTTAGTGT CTGCCAATAA GCTGACTCGA TACATAGAGC 
     1441 CCTGCCAGTT GACAGAAGAC TTTGGTGGGA GTCTCACCTA CGATCACATG GACTGGCTAA 
     1501 ATAAGAGGCT GGTCTTTGAG AAGTTTACAA AGGAGTCTAC ATCATTGCTA GATGAACTCG 
     1561 CTCTGATTAA CAATGGAAGT GACAAGGGGA ATGAGCAGGA GAAGGAAAGG TCCGTGGATT 
     1621 TAAACTTTCT TCCATCTGTT GATCCTGAGA CAGTTCTTCA GACAGGGCAT GAGTTGTTAT 
     1681 CTGAATTACA GCAGCGTCGA TTTAATGGCT CTGATGGAGG GGTCTCCTGG TCTCCTATGG 
     1741 ATGACGAACT GCTCGCACAG CCACAGGTTA TGAAGTTGTT AGACTCACTC CGGGAGCAGT 
     1801 ATACGCGCTA CCAGGAGGTC TGCAGGCAGC GCAGTAAGCG CACACAGTTA GAGGAGATCC 
     1861 AGCAGAAGGT GATGCAGGTT GTGAACTGGC TTGAAGGGCC TGGGTCTGAG CAACTGAGAG 
     1921 CACAGTGGGG CATTGGTGAC TCCATCAGGG CATCCCAGGC CCTGCAACAG AAGCATGAGG 
     1981 AGATCGAGAG CCAGCACAGT GAGTGGTTTG CAGTGTACGT GGAGCTCAAC CAACAGATCG 
     2041 CAGCGCTCCT GAATGCCGGA GATGAGGAAG ACCTTGTGGA GTTGAAGTCC CTGCAGCAGC 
     2101 AGCTCAGCGA CGTCTGCTAC CGGCAGGCCA GCCAGCTAGA ATTTAGGCAG AACCTCCTGC 
     2161 AGGCAGCTTT GGAGTTTCAT GGTGTGGCCC AAGATTTGTC TCAGCAGTTG GACGGCTTAT 
     2221 TAGGGATGTT GTGCGTAGAC GTAGCACCAG CTGATGGAGC ATCGATTCAG CAAACTTTAA 
     2281 AACTGCTTGA AGAGAAGCTG AAAAGTGTTG ATGTGGGATT GCAAGGCTTA CGAGAAAAAG 
     2341 GTCAAGGTCT CCTGGATCAG ATCTCCAATC AGGCTTCCTG GGCCTATGGA AAGGATGTAA 
     2401 CAATTGAGAA TAAAGAAAAC GTGGACCACA TACAAGGAGT GATGGAAGAT ATGCAACTTA 
     2461 GGAAACAAAG ATGTGAGGAC ATGGTAGACG TGCGGAGGCT GAAGATGCTT CAGATGGTGC 
     2521 AGTTGTTTAA ATGCGAAGAG GATGCTTCAC AGGCAGTAGA ATGGCTAAGT GAACTTCTGG 
     2581 ATGCCCTGTT GAAGACCCAT ATCAGATTGG GTGATGATGC TCAGGAAACA AAGGTTTTAC 
     2641 TGGAAAAACA CAGAAAATTT GTGGATGTTG CACAGAGCAC TTACGACTAT GGCAGACAGC 
     2701 TGCTGCAGGC CACAGTTGTG CTGTGCCAGT CTCTGCGCTG TACTTCCCGA TCCTCAGGGG 
     2761 ACACGCTTCC TCGACTGAAC AGAGTGTGGA AGCAGTTCAC GGTAGCCTCT GAAGAGAGAG 
     2821 TGCACAGGTT GGAAATGGCT ATTGCCTTCC ACTCGAATGC TGAAAAGATT TTGCAAGATT 
     2881 GCCCAGAAGA GCCTGAAGCA ATGAACGATG AGGAGCAGTT TGAGGAAATT GAAGCAATTG 
     2941 GAAAATCACT TCTGGATAGA CTCACTATCC CCGTCGTTTA CCCGGATGGA ACTGAACAAT 
     3001 ATTTTGGAAG TCCAAGTGAC ATGGCTTCTA CTGCAGAGCA CATCAGAGAC AGAATGAAAC 
     3061 TGGTCAGTCT CAAAAGGCAG CAGCTGAGAC ATCCTGAAAT GGTGACCACG GAGAGCACGC 
     3121 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3181 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3241 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3301 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3361 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3421 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3481 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3541 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3601 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3661 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3721 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     3781 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     3841 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     3901 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     3961 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4021 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4081 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4141 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4201 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4261 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4321 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4381 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4441 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4501 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4561 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4621 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4681 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4741 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     4801 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     4861 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     4921 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     4981 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5041 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5101 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5161 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5221 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5281 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5341 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5401 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5461 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5521 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5581 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5641 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5701 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     5761 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     5821 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     5881 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     5941 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6001 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6061 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6121 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6181 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6241 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6301 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6361 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6421 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6481 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6541 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6601 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6661 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6721 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     6781 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     6841 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     6901 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     6961 GCGAATTTT
//