LOCUS       RR207050                8454 bp    DNA     circular  SYN 
DEFINITION  Mtmr4 (Myc-DDK-tagged ORF) - Rat myotubularin related protein 4 (Mtmr4), (10 ug)
ACCESSION   NM_001105827
VERSION     NM_001105827.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4611..4702 
                     /label="Myc-DDK"  
     misc_feature    4732..5317 
                     /label="PolyA signal"  
     misc_feature    4789..4808 
                     /label="Restriction Enzyme"  
     misc_feature    5593..6265 
                     /label="ColE1"  
     misc_feature    6695..7489 
                     /label="Kan/Neo"  
     misc_feature    7524..7826 
                     /label="SV40 ori"  
     misc_feature    7912..8389 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4593..4599 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4593 
                     /label="Mtmr4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTGAGGAG GGCCCCCCCA GCCTGGAGTA CATCCAAGCC AAGGATCTGT 
     1081 TCCCCCCCAA GGAACTGGTG AAGGAGGAGG AAAATCTTCA GGTACCCTTC ACAGTGCTAC 
     1141 AGGGTGAGGG AGTGGAATTC CTGGGCCGGG CAGCTGATGC CCTCATTGCA ATCTCCAACT 
     1201 ACCGGCTGCA TATCAAGTTC AAAGACTCTG TCATCAACGT GCCCCTCCGT ATGATTGACA 
     1261 GTGTGGAAAG CCGTGATATG TTTCAGTTGC ACATTGCCTG CAAGGACTCT AAAGTGGTGA 
     1321 GGTGCCACTT CTCCACTTTC AAGCAGTGCC AAGAGTGGCT CTCAAGGCTA AGCCGGGCCA 
     1381 CAGCAAGACC TGCCAAGCCC GAGGATCTCT TTGCCTTTGC CTACCATGCC TGGTGCCTGG 
     1441 GGCTGACTGA GGAGGATCAG CATACCCACC TGTGTCAGCC AGGAGAGCAC ATCCGATGTC 
     1501 GACAGGAGGC CGAGCTCGCA AGGATGGGTT TTGACCTGCA GAATGTCTGG AGAGTCTCGC 
     1561 ATATCAACAG CAATTACAAG CTGTGCCCTA GCTACCCCCA GAAGCTGCTG GTTCCTGTGT 
     1621 GGATCACAGA TAAAGAGCTG GAGAATGTGG CTTCTTTCCG CTCTTGGAAG CGCATCCCTG 
     1681 TTGTGGTGTA CAGACACCTG CGCAACGGGG CTGCCATTGC CCGCTGCAGC CAGCCTGAGA 
     1741 TCAGCTGGTG GGGCTGGCGC AATGCTGATG ATGAATACCT CGTCACGTCC ATTGCCAAAG 
     1801 CCTGCGCCTT AGACCCAGGG ACCAGGGCCA GTGGGGGCTC CCTCAGTGCT GGGACTAATG 
     1861 ATGCCAGCGA GGCATGTGAC ACGGATTTCG ATTCTTCTCT GACTGCATGC TCTGGGGTAG 
     1921 AGAGCACAGC AGCCCCCCAA AAACTGCTGA TCCTGGATGC ACGGTCCTAC ACAGCAGCAG 
     1981 TGGCCAACCG GGCCAAGGGT GGAGGCTGTG AATGTGAAGA ATACTATCCG AACTGTGAGG 
     2041 TCGTGTTTAT GGGAATGGCC AACATCCATG CCATCCGGAA CAGCTTCCAG TACCTCCGGG 
     2101 CTGTGTGTAG CCAGATGCCA GATCCCAGCA ACTGGTTGTC GGCTCTGGAG AGTACCAAAT 
     2161 GGCTACAGCA CTTGTCAGTG ATGCTAAAAG CGGCTGTGTT GGTAGCCAAT ACAGTAGATC 
     2221 GGGAAGGCCG GCCTGTGCTG GTGCACTGCT CTGATGGCTG GGATCGTACG CCACAGATTG 
     2281 TAGCCCTGGC CAAAATACTA CTGGATCCGT ATTACAGGAC TCTGGAGGGC TTCCAAGTGT 
     2341 TAGTGGAGTC TGATTGGCTG GATTTTGGGC ACAAGTTTGG AGATCGCTGT GGCCATCAGG 
     2401 AAAATGCAGA GGACCAAAAT GAACAGTGTC CGGTGTTCCT CCAGTGGCTT GATTCTGTAC 
     2461 ATCAGCTGCT TAAGCAGTTC CCCTGCCTGT TTGAATTCAA TGAAGCATTT CTGGTAAAAC 
     2521 TGGTGCAGCA CACGTACTCC TGCCTCTACG GCACATTCCT GGCCAACAAC CCCTGCGAGC 
     2581 GAGAGAAGCG CAACATCTAT AAACGGACAT GCTCGGTGTG GGCACTCCTG CGAGCTGGCA 
     2641 ACAAGAACTT CCATAACTTC CTCTACACAC CTGGGTCAGA CATGGTCCTA CATCCCGTGT 
     2701 GTCACGTCCG GGCCCTGCAC CTCTGGACAG CTGTTTACCT GCCTGCGTCA TCGCCGTGCA 
     2761 CCCTTGGAGA GGAGAATATG GATCTTTACC TTTCCCCAGT GGCTCAGAGC CAGGAATTTT 
     2821 CTGGTCGTTC TCTGGACAGG TTACCTAAAA CCAGATCCAT GGATGATCTT CTTTCTGCCT 
     2881 GTGATACAAG CAGCCCCCTC ACTCGTACTT CCAGTGACCC GAACCTGAAT AACCACTGTC 
     2941 AGGAGGTCAG AGTCAGCCTG GAGCCCTGGC ACAGCAATCC TGAAGGAGCA GAGGCAGTCA 
     3001 TAGAATCTGG AGTCGGAAGC CCACAGCTGA CTGTGGGAGA CGTGGGCCTT CCTCCTCCTC 
     3061 TGCCCAGTAG CCAGAAAGAC TACTTGAGCA ATAAACCTTT CAAGGGTCAC AAAAGTTGTT 
     3121 CTCTTAGTTA TAAGTTGCTT AATACTTCTG TGTCCTGGGA AATGAAGAAC ACCTCTGAGA 
     3181 TCAAAGTCCT GGAAGAAACT GAAGCACTGG CCCCGGGCCC TCCAGCCCGA GAGGAGCAGA 
     3241 GTAGGACTTC TGATGGCTTA GGGAAGCCAC CTGAACAGTG TCCAGAGAAG GAAGCTACCA 
     3301 GCTCACTCTC CAATGTTTCC AGCAAGTGTG GAGGAGCCTG TGATTTTCCT GGGGAGCCTC 
     3361 CCCAGGATTC CTTTACAGGT ACTCCCCAAC AGCCCCATCT AGACTCCATG CAGGCTGTGC 
     3421 CCATCAGGTG TACTCCAGAT CACGGTCAGG GCAGCCTTTG CAACCCACCA AGTGCTGCCA 
     3481 GCCAAACTCT TCAAGAGCCA AATACTGACC TCATCAGCCA AGATCCCCCT GGGTCTATGG 
     3541 CAGGTATCTC CTACCAGGAA CAGCCTAGTT CTGTGTCGGA TCTGATCTAT AAGAAGGAAG 
     3601 ATGCTGGCAA GAGAGGAAGT AAAAATGGGC AGTTGGTGGA AAATCCTCGC TTTGGGAAAA 
     3661 TGCCATTAGA GTTGGCCCGA AAGCCAATTT CTCAGAGCCA GATCAGTGAG TTCTCATTTT 
     3721 TAGGGTCAAA CTGGGACAGC TTCCAAGGGA TGATGACGTC ATTCCCCAGT GGAGAGACCA 
     3781 CTCCTCGGCG GCTGCTTGCT TATGGCTGTT GTAGCAAGAG GCCGAGCAAT AAGCATATCC 
     3841 GAGCTGCAGC GCCCTGCTTT GGGGGCCAGT GGGCTCAGAG AGAAGGGATG AAGTCGCCCG 
     3901 TCTGCTCTAG TCATTCCAAT GGACACTGTA CAGGCCCAGG AGGAAAGAAC AACCGGATGT 
     3961 GGTTTTCCAG TCATCCAAAG CAAGTCTCCA GCACAAAGCC TTCTCTTCTG AGCTGCCCTT 
     4021 CTCCAGTGCC TCCTCTCTAC CTGGATGATG ATGGACTCCC CTTTCCCACG GATGTGATCC 
     4081 AACATAGGTT ACGGCAGATC GAAGCAGGGT ACAAGCAAGA GGTAGAACAG CTACGTCGAC 
     4141 AGGTTCGAGA GCTTCAGATG AGGCTAGACA TTCGTCACTG CTGTGCCCCT CCAGCAGAGC 
     4201 CCCCCATGGA CTATGAGGAT GACTTTACGT GTTTAAAGGA GTCAGATGGC AGTGACACTG 
     4261 AAGACTTTGG CTCTGATCAC AGTGAGGACT GCCTTTCAGA AGCAAGCTGG GAACCTGTTG 
     4321 ATAAGAAGGA GACGGAGGTG ACTCGCTGGG TTCCAGACCA TATGGCATCA CACTGCTATA 
     4381 ACTGTGACTG TGAATTCTGG TTGGCCAAAC GAAGGCATCA TTGCAGAAAT TGTGGGAACG 
     4441 TATTTTGTGC TGGATGCTGC CACCTTAAGC TGCCCATTCC CGATCAGCAA CTCTATGACC 
     4501 CAGTTCTCGT CTGTAACTCA TGTTACGAGC ACATTCAAGT GTCTCGTGCC AGGGAGCTCA 
     4561 TGAGCCAACA TCTGAAGAAA CCCATTGCTA CAGCTTCCAG TACGCGTACG CGGCCGCTCG 
     4621 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4681 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4741 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4801 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4861 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4921 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4981 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5041 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5101 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5161 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5221 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5281 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5341 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5401 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5461 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5521 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5581 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5641 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5701 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5761 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5821 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5881 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5941 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6001 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6061 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6121 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6181 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6241 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6301 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6361 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6421 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6481 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6541 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6601 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6661 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6721 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6781 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6841 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6901 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6961 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7021 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7081 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7141 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7201 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7261 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7321 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7381 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7441 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7501 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7561 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7621 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7681 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7861 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7921 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7981 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8041 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8101 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8161 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8221 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8281 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8341 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8401 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//