LOCUS RR207028 6507 bp DNA circular SYN DEFINITION Nefl (Myc-DDK-tagged ORF) - Rat neurofilament, light polypeptide (Nefl), (10 ug) ACCESSION NM_031783 VERSION NM_031783.2 SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 2664..2755 /label="Myc-DDK" misc_feature 2785..3370 /label="PolyA signal" misc_feature 2842..2861 /label="Restriction Enzyme" misc_feature 3646..4318 /label="ColE1" misc_feature 4748..5542 /label="Kan/Neo" misc_feature 5577..5879 /label="SV40 ori" misc_feature 5965..6442 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 2646..2652 /label="Restriction Enzyme" misc_feature 1020..2646 /label="Nefl" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GAGTTCGTTC AGCTACGAGC CGTACTTTTC GACCTCCTAC AAGCGGCGCT 1081 ACGTGGAGAC GCCCCGGGTG CACATCTCCA GCGTGCGCAG CGGCTACAGC ACGGCGCGCT 1141 CTGCGTACTC CAGCTACTCC GCGCCCGTCT CCTCCTCTCT GTCGGTGCGC CGCAGCTACT 1201 CATCCAGCTC CGGCTCTTTG ATGCCCAGCC TGGAGAACCT CGATCTGAGC CAGGTAGCCG 1261 CCATCAGCAA CGACCTCAAG TCTATCCGCA CACAGGAGAA GGCACAGCTG CAGGACCTCA 1321 ACGATCGCTT CGCCAGCTTC ATCGAGCGCG TGCACGAGCT GGAGCAGCAG AACAAGGTCC 1381 TGGAAGCCGA GCTGTTGGTG CTGCGCCAGA AGCACTCAGA GCCTTCCCGC TTCCGCGCCC 1441 TGTATGAGCA GGAGATCCGT GATCTGCGAC TGGCGGCCGA AGACGCCACT AACGAGAAGC 1501 AGGCGCTGCA GGGCGAGCGC GAGGGGCTGG AGGAGACTCT GCGCAACCTG CAGGCTCGCT 1561 ACGAGGAGGA GGTGCTGAGC CGCGAGGACG CCGAGGGCCG GCTGATGGAA GCCCGCAAAG 1621 GCGCGGATGA GGCTGCGCTC GCCCGCGCCG AGCTGGAGAA GCGCATCGAC AGCCTGATGG 1681 ACGAGATAGC CTTCCTGAAA AAGGTGCACG AGGAAGAGAT CGCCGAGCTG CAGGCTCAGA 1741 TCCAGTATGC TCAGATCTCC GTGGAGATGG ACGTGTCCTC CAAGCCCGAC CTCTCCGCCG 1801 CTCTCAAGGA CATCCGCGCT CAGTACGAGA AGCTGGCCGC CAAGAATATG CAGAATGCCG 1861 AAGAGTGGTT CAAGAGCCGC TTCACGGTGC TAACCGAGAG CGCCGCCAAG AACACCGACG 1921 CAGTGCGCGC TGCCAAGGAC GAGGTGTCGG AAAGCCGCCG CCTACTTAAG GCTAAGACCC 1981 TGGAGATCGA AGCCTGCCGG GGTATGAACG AAGCTCTAGA GAAGCAGCTG CAGGAGCTGG 2041 AGGACAAGCA GAATGCAGAC ATCAGCGCCA TGCAGGACAC AATCAACAAA CTGGAGAATG 2101 AGCTGCGAAG CACGAAGAGC GAGATGGCCA GGTACCTGAA GGAGTACCAG GACCTCCTCA 2161 ATGTCAAGAT GGCATTGGAC ATTGAGATTG CAGCTTACAG GAAACTCTTG GAAGGCGAAG 2221 AAACCAGGCT CAGTTTCACC AGCGTGGGTA GCATAACCAG CGGCTACTCT CAGAGCTCGC 2281 AGGTCTTTGG CCGTTCTGCT TACAGTGGCT TGCAGAGCAG CTCCTACTTG ATGTCTGCTC 2341 GAGCATTCCC AGCCTACTAT ACCAGCCACG TCCAGGAAGA GCAGTCAGAG GTGGAGGAGA 2401 CCATTGAGGC TACGAAAGCT GAGGAGGCCA AGGATGAGCC CCCCTCTGAA GGAGAAGCAG 2461 AAGAGGAGGA GAAGGAGAAA GAGGAGGGGG AGGAAGAGGA AGGTGCTGAG GAGGAAGAAG 2521 CTGCCAAGGA TGAGTCTGAA GATGCCAAAG AAGAAGAAGG TGGTGAGGGT GAAGAGGAAG 2581 ACACCAAAGA GTCAGAAGAG GAAGAGAAGA AAGAGGAAAG TGCTGGGGAG GAGCAAGCTG 2641 CTAAGAAGAA AGATACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 2701 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 2761 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 2821 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 2881 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 2941 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 3001 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 3061 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 3121 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 3181 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 3241 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 3301 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 3361 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 3421 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 3481 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 3541 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 3601 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 3661 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 3721 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 3781 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 3841 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 3901 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 3961 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 4021 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 4081 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 4141 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 4201 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 4261 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 4321 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 4381 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 4441 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 4501 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 4561 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 4621 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 4681 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 4741 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 4801 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 4861 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 4921 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 4981 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 5041 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 5101 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 5161 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 5221 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 5281 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 5341 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 5401 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 5461 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 5521 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 5581 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 5641 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 5701 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 5761 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 5821 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 5881 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 5941 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 6001 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 6061 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 6121 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 6181 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 6241 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 6301 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 6361 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 6421 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 6481 ATTTAACAAA AATTTAACGC GAATTTT //