LOCUS       RR207014                8154 bp    DNA     circular  SYN 
DEFINITION  Pdgfra (Myc-DDK-tagged ORF) - Rat platelet derived growth factor receptor, alpha polypeptide (Pdgfra), (10 ug)
ACCESSION   NM_012802
VERSION     NM_012802.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4309..4400 
                     /label="Myc-DDK"  
     misc_feature    4430..5015 
                     /label="PolyA signal"  
     misc_feature    4487..4506 
                     /label="Restriction Enzyme"  
     misc_feature    5291..5963 
                     /label="ColE1"  
     misc_feature    6393..7187 
                     /label="Kan/Neo"  
     misc_feature    7222..7524 
                     /label="SV40 ori"  
     misc_feature    7610..8087 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4284..4291 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4284 
                     /label="Pdgfra"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGACCTCC CAGGCCTTTC TGGTCCTCAG CTGTCTCCTC ACAGGGCCGA 
     1081 GCCTCATCGT CTGCCAGCTC TTATTACCCT CCATCCTTCC AAATGAGAAT GAGAAGATTG 
     1141 TGCCGCTGAG TTCGTCCTTC TCTCTGAGAT GCTTCGGGGA GAGCGAAGTG AGCTGGCAGC 
     1201 ACCCCATGTC TGAAGAAGAG GACCCCAACG TGGAAATCAG AACCGAGGAG AACAACAGTA 
     1261 GCCTCTTTGT CACGGTGCTG GAAGTGGTCA ACGCCTCAGC GGCCCACACC GGATGGTACA 
     1321 CTTGCTATTA CAACCACACT CAGACAGAAG AGAGTGAGAT CGAAGGCAGG CACATTTATA 
     1381 TCTATGTACC AGACCCAGAC ATGGCCTTCG TGCCTCTCGG CATGACGGAT TCTTTAGTCA 
     1441 TTGTGGAAGA GGACGATTCT GCCATCATAC CCTGCCGCAC CACGGATCCA GACACTGAAG 
     1501 TAACCTTGCA CAATAACGGG AGGCTGGTGC CTGCCTCCTA CGACAGCAGG CAGGGCTTCA 
     1561 ACGGAACCTT CAGTGTGGGG CCTTACATCT GTGAGGCCAC CGTCAGAGGG AGGACGTTCA 
     1621 AGACCAGCGA GTTTAACGTT TATGCCTTGA AAGCCACGTC AGAACTGAAT CTGGAGATGG 
     1681 ACACACGCCA GACTGTGTAT AAGGCAGGAG AAACGATCGT GGTGACCTGC GCCGTCTTTA 
     1741 ACAATGAGGT GGTTGACCTG CAGTGGACTT ACCCTGGAGA GGTGAGAAAC AAAGGCATCA 
     1801 CCATGCTGGA GGAGATCAAA CTCCCGTCCA TCAAACTGGT GTACACGCTG ACCGTCCCCA 
     1861 AGGCCACGGT GAAGGACAGT GGAGATTATG AATGTGCTGC CCGCCAGGCC ACGAAAGAGG 
     1921 TCAAGGAAAT GAAGACAGTC ACCATTTCTG TTCACGAGAA AGGCTTCGTC CAGATCAGGC 
     1981 CCACCTTTGG CCATCTGGAA ACTGTGAACC TGCATCAGGT CAGAGAGTTC GTGGTGGAGG 
     2041 TGCAGGCCTA CCCGACCCCC AGGATATCCT GGCTGAAGGA CAACTTGACC CTGATTGAGA 
     2101 ATCTCACTGA GATCACCACC GATGTGCAGA GGAGCCAGGA GACGAGGTAT CAAAGCAAAT 
     2161 TAAAGCTGAT CCGGGCTAAG GAAGAAGACA GCGGCCATTA TACCATTATA GTTCAGAATG 
     2221 ACGACGACAT GAAGAGCTAC ACGTTTGAGC TGTCAACTCT AGTTCCTGCC TCCATTCTGG 
     2281 AGCTTGTGGA TGACCACCAT GGCTCTGGCG GGGGACAGAC TGTGAGGTGC ACGGCTGAAG 
     2341 GCACCCCTCT TCCGAACATC GAGTGGATGA TCTGCAAGGA TATTAAGAAA TGTAATAATG 
     2401 ACACTTCATG GACAGTTTTG GCCAGCAACG TCTCGAATAT TATCACGGAG TTCCACCAGC 
     2461 GAGGCAGGAG TACAGTGGAG GGACGAGTGT CCTTCGCCAA GGTGGAAGAG ACCATCGCAG 
     2521 TTCGGTGCTT GGCAAAGAAC GACCTTGGCA TTGGGAACAG AGAGCTGAAG CTGGTGGCTC 
     2581 CCACCCTGCG ATCTGAGCTC ACGGTGGCGG CCGCGGTGCT GGTGCTGTTG GTGATTGTCA 
     2641 TTGTCTCTCT CATTGTCCTG GTTGTCATTT GGAAGCAGAA ACCACGGTAT GAAATTCGAT 
     2701 GGAGGGTCAT TGAGTCAATC AGCCCTGATG GACATGAGTA TATTTATGTG GACCCCATGC 
     2761 AGCTGCCTTA CGACTCCAGA TGGGAGTTCC CGAGAGATGG ACTAGTGCTT GGTCGGATCT 
     2821 TGGGATCCGG TGCATTTGGG AAAGTGGTTG AAGGCACAGC TTATGGATTA AGCCGGTCCC 
     2881 AGCCTGTAAT GAAGGTGGCT GTGAAGATGC TCAAACCCAC AGCCAGGTCT AGTGAGAAGC 
     2941 AAGCTCTCAT GTCTGAGCTG AAGATAATGA CTCACTTGGG ACCGCATCTG AACATTGTGA 
     3001 ATTTGCTGGG AGCCTGCACC AAGTCAGGCC CCATTTACAT CATCACAGAA TACTGCTTCT 
     3061 ATGGGGATTT GGTCAATTAC CTGCATAAGA ACAGAGATAG CTTCATGAGC CGACACCCAG 
     3121 AGAAGCCAAA GAAAGACCTG GACATCTTTG GATTGAATCC TGCAGATGAG AGTACCAGGA 
     3181 GTTACGTGAT TTTATCTTTT GAAAACAATG GTGACTACGT GGACATGAAG CAGGCTGACA 
     3241 CCACACAGTA CGTGCCAATG CTGGAAAGGA AGGAGGTTTC CAAATACTCC GACATCCAGA 
     3301 GATCGCTGTA CGATCGGCCG GCCTCCTATA AGAAGAAATC CATGCTAGAC TCAGAAGCTA 
     3361 AAAACCTCCT TTCGGATGAT GACTCCGAGG GTCTGACTTT GCTGGACCTA CTAAGCTTCA 
     3421 CCTACCAAGT GGCCCGAGGG ATGGAATTTT TGGCTTCGAA AAATTGTGTC CACCGGGACC 
     3481 TGGCTGCCCG CAACGTCCTT CTGGCGCAGG GGAAAATCGT GAAGATCTGT GACTTCGGGC 
     3541 TGGCCAGAGA CATCATGCAC GATTCTAACT ACGTGTCAAA GGGCAGCACT TTCCTGCCTG 
     3601 TGAAGTGGAT GGCACCTGAG AGCATTTTTG ACAACCTCTA CACCACGCTG AGTGACGTCT 
     3661 GGTCTTATGG CGTTCTGCTC TGGGAAATAT TCTCCCTTGG TGGCACACCC TACCCTGGCA 
     3721 TGATGGTTGA TTCTACTTTC TACAATAAGA TCAAGAGCGG ATACAGGATG GCCAAACCCG 
     3781 ACCATGCCAC CAGTGAAGTC TATGAGATTA TGGTGCAGTG CTGGAACAGT GAACCCGAAA 
     3841 AGAGACCCTC CTTCTACCAC CTCAGCGAGA TAGTGGAGAA TCTGCTGCCT GGACAGTACA 
     3901 AAAAGAGTTA TGAAAAGATT CACCTGGACT TCCTAAAGAG TGACCATCCG GCTGTGGCAC 
     3961 GCATGCGTGT GGACTCTGAC AACGCGTACA TCGGTGTCAC TTACAAAAAT GAAGAGGACA 
     4021 AGCTGAAGGA ATGGGAAGGT GGCCTGGATG AACAGAGATT GAGCGCAGAT AGTGGCTACA 
     4081 TCATTCCCCT GCCAGACATT GACCCTGTTC CAGAGGAGGA AGACCTGGGC AAGAGGAACA 
     4141 GACACAGCTC GCAGACTTCG GAAGAGAGCG CCATCGAGAC GGGTTCCAGT AGTTCCACTT 
     4201 TCATCAAGAG AGAGGACGAG ACCATTGAGG ACATCGACAT GATGGATGAT ATTGGCATAG 
     4261 ATTCCTCAGA CCTGGTGGAG GACAGCTTCC TGAGCGGACC GACGCGTACG CGGCCGCTCG 
     4321 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4381 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4441 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4501 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4561 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4621 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4681 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4741 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4801 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4861 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4921 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4981 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5041 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5101 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5161 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5221 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5281 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5341 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5401 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5461 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5521 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5581 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5641 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5701 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5761 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5821 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5881 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5941 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6001 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6061 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6121 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6181 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6241 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6301 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6361 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6421 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6481 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6541 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6601 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6661 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6721 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6781 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6841 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6901 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6961 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7021 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7081 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7141 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7201 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7261 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7321 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7381 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7441 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7501 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7561 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7621 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7681 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7741 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7801 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7861 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7921 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7981 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8041 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8101 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//