LOCUS       RR206917                7319 bp    DNA     circular  SYN 
DEFINITION  Clcn7 (Myc-DDK-tagged ORF) - Rat chloride channel 7 (Clcn7), (10 ug)
ACCESSION   NM_031568
VERSION     NM_031568.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3476..3567 
                     /label="Myc-DDK"  
     misc_feature    3597..4182 
                     /label="PolyA signal"  
     misc_feature    3654..3673 
                     /label="Restriction Enzyme"  
     misc_feature    4458..5130 
                     /label="ColE1"  
     misc_feature    5560..6354 
                     /label="Kan/Neo"  
     misc_feature    6389..6691 
                     /label="SV40 ori"  
     misc_feature    6777..7254 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3458..3464 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3458 
                     /label="Clcn7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAACGTT TCTAAGAAAG TGTCTTGGTC CGGCCGAGAT CGCGATGACG 
     1081 AGGAGGGGGC GCCGCTGCTT CGAAGGACGG GGCAACCTGA CGAGGAGACG CCGCTGCTGA 
     1141 ACGGAGCCGG GCCGGGCGCG CGCCAGTCTC ATTCTGCACT TTTCCGAATT GGACAGATGA 
     1201 ACAACGTGGA GCTGGATGAT GAACTCCTGG ACCCGGAAGT GGACCCTCCT CACACCTTCC 
     1261 CCAAGGAGAT TCCACACAAC GAGAAGCTCC TCTCCCTCAA GTATGAGAGC CTGGACTATG 
     1321 ACAATAGTGA GAATCAGCTC TTCCTGGAGG AGGAAAGACG AATCAACCAC ACGGCTTTCC 
     1381 GGACAGTGGA GATCAAGCGC TGGGTTATCT GTGCCCTCAT TGGAATCCTC ACAGGCCTAG 
     1441 TAGCCTGCTT CATTGACATT GTAGTGGAGA ACCTGGCAGG CCTCAAGTAC CGAGTCATCA 
     1501 AGGACAACAT CGACAAGTTC ACAGAGAAGG GCGGCCTGTC CTTCTCCCTC CTGCTGTGGG 
     1561 CCACACTGAA CTCTGCCTTC GTGCTCGTGG GGTCTGTGAT TGTGGCCTTC ATAGAGCCAG 
     1621 TTGCTGCTGG CAGCGGAATC CCTCAGATCA AGTGCTTCCT CAATGGGGTG AAGATCCCCC 
     1681 ACGTGGTGCG GCTCAAGACG CTGGTGATCA AGGTGTCTGG CGTGATTCTG TCTGTGGTAG 
     1741 GGGGACTGGC TGTGGGAAAG GAAGGGCCAA TGATCCACTC AGGATCCGTG ATTGCTGCAG 
     1801 GGATTTCACA GGGAAGGTCG ACGTCACTCA AGCGAGATTT TAAGATCTTT GAATATTTCC 
     1861 GCAGAGATAC AGAGAAGCGG GATTTTGTCT CAGCTGGAGC TGCAGCTGGA GTGTCTGCTG 
     1921 CGTTTGGAGC ACCTGTGGGT GGGGTCCTGT TCAGCCTGGA AGAGGGCGCC TCCTTCTGGA 
     1981 ATCAGTTCCT GACATGGAGA ATTTTCTTTG CTTCCATGAT TTCGACCTTT ACACTGAATT 
     2041 TTGTTCTGAG CATCTACCAT GGAAACATGT GGGACCTGTC CAGCCCTGGC CTCATAAATT 
     2101 TTGGAAGATT CGACTCAGAG AAAATGGCCT ACACAATCCA TGAGATTCCT GTCTTCATCG 
     2161 CCATGGGTGT GGTGGGTGGC ATTCTTGGAG CCGTGTTCAA TGCCTTGAAT TACTGGCTAA 
     2221 CTATGTTTCG AATCAGGTAC ATCCACCGGC CCTGCCTCCA AGTGATTGAG GCCATGCTGG 
     2281 TGGCAGCTGT CACAGCCACA GTTGCATTTG TCTTGATTTA CTCGTCTCGA GATTGCCAGC 
     2341 CCCTGCAGGG GAGCTCCATG TCCTACCCAC TCCAGCTCTT CTGTGCAGAT GGCGAATACA 
     2401 ACTCAATGGC CGCAGCCTTC TTTAACACCC CTGAGAAGAG CGTCGTCAGC CTGTTCCACG 
     2461 ACCCACCAGG CTCCTATAAT CCCATGACTC TCGGCCTGTT CACCCTGGTC TACTTCTTCC 
     2521 TGGCCTGCTG GACCTATGGC CTCACAGTAT CTGCTGGTGT CTTCATCCCA TCCCTGCTCA 
     2581 TTGGGGCTGC CTGGGGCCGA CTCTTTGGCA TCTCCATGTC CTACCTCACA GGAGCAGCGA 
     2641 TCTGGGCAGA TCCGGGTAAA TACGCCCTGA TGGGAGCTGC TGCTCAGCTT GGTGGGATCG 
     2701 TGAGGATGAC CCTTAGCCTG ACAGTCATCA TGATGGAGGC CACCAGCAAC GTGACCTACG 
     2761 GTTTTCCCAT CATGTTGGTG CTGATGACTG CCAAGATTGT GGGTGATGTC TTCATTGAGG 
     2821 GCCTCTATGA CATGCACATC CAGCTGCAAA GTGTGCCCTT CCTACACTGG GAAGCCCCGG 
     2881 TCACCTCACA TTCGCTCACT GCCAGGGAAG TAATGAGCAC GCCTGTGACC TGCCTGAGGA 
     2941 GGAGAGAGAA GGTTGGCATC ATCGTGGATG TCCTAAGTGA CACAGCGTCT AATCACAATG 
     3001 GGTTCCCTGT GGTGGAGGAT GTAGGAGACA CCCAGCCAGC CAGACTCCAA GGCCTAATCC 
     3061 TGCGTTCCCA GCTCATCGTG CTCCTGAAGC ACAAGGTGTT TGTGGAGAGG TCCAACATGG 
     3121 GTTTGGTGCA GCGGAGACTG AGGCTGAAAG ACTTTCGCGA TGCCTACCCA CGCTTCCCCC 
     3181 CAATCCAGTC CATCCACGTA TCCCAGGATG AGCGGGAGTG CACCATGGAC CTTTCTGAGT 
     3241 TCATGAACCC TTCTCCCTAC ACTGTGCCAC AGGAGGCATC TCTTCCTCGA GTGTTCAAGC 
     3301 TGTTCCGGGC TCTGGGCCTG AGGCACCTGG TCGTAGTAGA CAACCACAAT CAGGTGGTCG 
     3361 GGCTGGTGAC CAGGAAGGAC CTAGCAAGAT ACCGCCTAGG AAAAGGAGGC CTAGAAGAGC 
     3421 TTTCACTGGC CCAGACGGCC TAGAAGAGCT TTCACTGGCC CAGACGACGC GTACGCGGCC 
     3481 GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA 
     3541 GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC 
     3601 CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC 
     3661 CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT 
     3721 CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA 
     3781 CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT 
     3841 GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT 
     3901 TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC 
     3961 GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC 
     4021 CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC 
     4081 TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC 
     4141 ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT 
     4201 CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC 
     4261 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 
     4321 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 
     4381 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 
     4441 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 
     4501 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 
     4561 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 
     4621 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 
     4681 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 
     4741 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 
     4801 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 
     4861 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 
     4921 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 
     4981 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 
     5041 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 
     5101 TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG 
     5161 AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG 
     5221 TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC 
     5281 CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC 
     5341 ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG 
     5401 TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG 
     5461 AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT 
     5521 GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT 
     5581 CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA 
     5641 CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG 
     5701 CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC 
     5761 GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT 
     5821 CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT 
     5881 GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT 
     5941 CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC 
     6001 GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA 
     6061 GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT 
     6121 CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT 
     6181 CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT 
     6241 GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT 
     6301 AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA 
     6361 TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA 
     6421 AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA 
     6481 TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG 
     6541 GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT 
     6601 TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG 
     6661 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC 
     6721 TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT 
     6781 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     6841 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     6901 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     6961 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     7021 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     7081 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     7141 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     7201 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     7261 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//