LOCUS       RR206876                6263 bp    DNA     circular  SYN 
DEFINITION  Ilk (Myc-DDK-tagged ORF) - Rat integrin linked kinase (Ilk), (10 ug)
ACCESSION   NM_133409
VERSION     NM_133409.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2420..2511 
                     /label="Myc-DDK"  
     misc_feature    2541..3126 
                     /label="PolyA signal"  
     misc_feature    2598..2617 
                     /label="Restriction Enzyme"  
     misc_feature    3402..4074 
                     /label="ColE1"  
     misc_feature    4504..5298 
                     /label="Kan/Neo"  
     misc_feature    5333..5635 
                     /label="SV40 ori"  
     misc_feature    5721..6198 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2402..2408 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2402 
                     /label="Ilk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGACATT TTCACTCAGT GCCGGGAGGG CAACGCGGTG GCGGTGCGCT 
     1081 TGTGGCTGGA CAACACAGAG AACGACCTAA ATCAGGGGGA TGATCATGGC TTCTCCCCTT 
     1141 TGCACTGGGC CTGCCGAGAA GGCCGATCTG CAGTGGTTGA AATGCTGATC ATGCGGGGAG 
     1201 CACGGATCAA TGTGATGAAT CGTGGGGATG ATACCCCCCT GCACTTGGCA GCTAGTCACG 
     1261 GACACCGTGA CATTGTACAG AAGCTGTTGC AATACAAGGC TGACATCAAT GCAGTGAATG 
     1321 AACATGGGAA TGTGCCCCTT CACTATGCAT GTTTCTGGGG CCAAGACCAG GTGGCAGAGG 
     1381 ACCTAGTAGC TAACGGGGCT CTTGTGAGCA TCTGTAACAA GTATGGAGAG ATGCCTGTGG 
     1441 ACAAAGCCAA GGCACCCCTT CGAGAGCTTC TCCGAGAACG GGCAGAGAAG ATGGGCCAGA 
     1501 ATCTTAATCG TATTCCATAC AAGGACACAT TCTGGAAGGG GACCACTCGC ACAAGGCCCC 
     1561 GAAATGGGAC CCTGAACAAA CACTCTGGTA TTGACTTCAA ACAGCTCAAC TTTCTGGCAA 
     1621 AGCTCAACGA GAATCATTCT GGAGAGCTAT GGAAAGGCCG CTGGCAGGGC AATGACATTG 
     1681 TTGTGAAGGT GCTGAAGGTT CGAGACTGGA GTACAAGGAA AAGCAGGGAC TTCAATGAGG 
     1741 AATGTCCCCG GCTCAGGATT TTCTCTCATC CTAACGTGCT TCCGGTACTA GGTGCTTGCC 
     1801 AGGCTCCCCC AGCTCCCCAC CCAACCCTCA TCACACACTG GATGCCATAT GGATCTCTCT 
     1861 ACAATGTTCT ACATGAAGGC ACCAATTTCG TTGTGGACCA GAGCCAAGCT GTAAAGTTTG 
     1921 CTTTGGACAT GGCAAGAGGC ATGGCTTTTC TTCATACACT AGAGCCTCTC ATACCTCGGC 
     1981 ACGCACTCAA TAGCCGTAGT GTAATGATCG ATGAAGATAT GACTGCCCGA ATCAGCATGG 
     2041 CTGATGTAAA GTTTTCTTTC CAGTGCCCTG GGCGCATGTA TGCACCTGCC TGGGTGGCCC 
     2101 CTGAAGCCCT GCAAAAGAAG CCTGAAGATA CAAACAGACG CTCAGCAGAC ATGTGGAGTT 
     2161 TTGCCGTGCT TCTGTGGGAA CTGGTGACAC GAGAGGTGCC CTTTGCTGAC CTTTCTAATA 
     2221 TGGAGATTGG AATGAAGGTG GCACTGGAAG GCCTTCGGCC TACTATCCCA CCAGGTATTT 
     2281 CCCCCCACGT GTGTAAGCTC ATGAAGATTT GCATGAATGA AGACCCTGCA AAGCGACCCA 
     2341 AGTTTGACAT GATTGTACCT ATCTTGGAGA AGATGCAGGA CAAGCTATCT TGGAGAAGAT 
     2401 GCAGGACAAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC 
     2461 AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG 
     2521 TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC 
     2581 TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT 
     2641 GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT 
     2701 GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA 
     2761 GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA 
     2821 TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT 
     2881 AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT 
     2941 CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA 
     3001 CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC 
     3061 AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG 
     3121 GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC 
     3181 CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 
     3241 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 
     3301 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 
     3361 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 
     3421 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 
     3481 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 
     3541 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 
     3601 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 
     3661 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 
     3721 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT 
     3781 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 
     3841 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 
     3901 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 
     3961 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 
     4021 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG 
     4081 CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT 
     4141 GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA 
     4201 GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT 
     4261 TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT 
     4321 GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG 
     4381 AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG 
     4441 GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC 
     4501 CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT 
     4561 CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT 
     4621 CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC 
     4681 CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT 
     4741 CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA 
     4801 GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG 
     4861 CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG 
     4921 TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG 
     4981 CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT 
     5041 CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA 
     5101 GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT 
     5161 TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC 
     5221 CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC 
     5281 CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA 
     5341 TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG 
     5401 GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA 
     5461 TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT 
     5521 GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT 
     5581 CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG 
     5641 CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG 
     5701 GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     5761 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     5821 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     5881 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     5941 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     6001 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     6061 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     6121 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     6181 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     6241 AACAAAAATT TAACGCGAAT TTT
//