LOCUS       RR206864                7482 bp    DNA     circular  SYN 
DEFINITION  Xylt2 (Myc-DDK-tagged ORF) - Rat xylosyltransferase II (Xylt2), (10 ug)
ACCESSION   NM_022296
VERSION     NM_022296.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3639..3730 
                     /label="Myc-DDK"  
     misc_feature    3760..4345 
                     /label="PolyA signal"  
     misc_feature    3817..3836 
                     /label="Restriction Enzyme"  
     misc_feature    4621..5293 
                     /label="ColE1"  
     misc_feature    5723..6517 
                     /label="Kan/Neo"  
     misc_feature    6552..6854 
                     /label="SV40 ori"  
     misc_feature    6940..7417 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    3621..3627 
                     /label="Restriction Enzyme"  
     misc_feature    1029..3621 
                     /label="Xylt2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GTGGCGAGCG CGCGGGTGCA GAAGCTGGTG CGGCGCTACA 
     1081 AGCTGGCGAT CGCCACCGCG CTGGCCATCC TGCTGCTGCA GGGCCTGGTG GTGTGGAGCT 
     1141 TCAGCGGCCT GGAGGAGGAC GAGCCGGGCG AGAAAGGAAG GCAGAGAAAG CCACGGCCGC 
     1201 TAGACCCTGG AGAGGGTTCC AAGGACACGG ACAGCTCTGC AGGGCGTAGG GGCAGTGCTG 
     1261 GGAGAAGGCA CGGACGGTGG CGGGGCCGCG CCGAGAGCCC AGGTGTGCCT GTGGCCAAGG 
     1321 TGGTGCGGGC AGTAACCAGT CGGCAGAGAG CCAGCCGACG GGTGCCCCCT GCCCCACCTC 
     1381 CAGAGGCACC AGGCCGCCAG AACCTGAGTG GAGCAGCAGC CGGGGAAGCA CTGATAGGTG 
     1441 CCGCCGGCTT CCCACAACAT GGAGACACAG GGAGTGTGGA GGGTGCCCCT CAGCCCACGG 
     1501 ATAACAGCTT CACTCCGAAG TGTGAGATTG TGGGCAAGGA TGCACTGTCA GCACTGGCCC 
     1561 GGGCCAGCAC CAAGCATTGT CAACAAGAGA TTGCTAATGT AGTGTGCCTG CACCAAGCTG 
     1621 GGAACCTAAT GCCCAAGTCT GTGCCCCGGC ACTGCCAGCT GGCTGGCAAG GTGAGCCCTG 
     1681 GCATCCAGTG GGAAGAGGTC CGGGCCCAGC AGCCTGTGAG TGGCCCTCTG GTACGCATCG 
     1741 CCTACATGCT GGTGGTTCAC GGCCGTGCTG TGCGCCAGCT GAAGCGTCTT CTGAAGGCCG 
     1801 TCTACCACGA GGAGCACTTC TTTTATATTC ATGTGGACAA GCGTTCCAAC TACCTGTACC 
     1861 GGGAGGTGGT AGAGCTGGCC CAGCACTACG ACAATGTACG GGTAACACCT TGGCGCATGG 
     1921 TCACCATCTG GGGTGGGGCT AGCCTTCTGA GGATGTACCT GCGGAGCATG AAGGACCTTC 
     1981 TGGAGATTCC TGGCTGGACC TGGGACTTCT TCATCAACCT GAGCGCTACT GACTATCCAA 
     2041 CCAGGACGAA TGAGGAGCTG GTAGCGTTCT TATCCAAGAA CCGGGACAAG AATTTCCTCA 
     2101 AGTCACACGG GCGAGACAAT TCCAGGTTCA TCAAGAAGCA AGGCCTGGAC CGGCTTTTCC 
     2161 ATGAGTGTGA TTCCCACATG TGGCGCCTGG GTGAACGGCA GATCCCGGCA GGCATCGTGG 
     2221 TGGATGGTGG CTCTGACTGG TTCGTGCTGA CACGCAGCTT TGTGGAATAT GTGGTGTATA 
     2281 CAGAGGATCC TCTGGTGGCC CAGCTTCGCC AGTTCTATAC ATACACATTG CTTCCAGCCG 
     2341 AGTCCTTCTT CCACACAGTG CTGGAGAACA GCCCAGCCTG TGAGAGCCTA GTGGACAACA 
     2401 ACCTGCGGGT TACCAACTGG AACCGGAAGC TGGGCTGCAA GTGCCAGTAC AAGCACATCG 
     2461 TGGACTGGTG TGGCTGCTCC CCCAACGACT TCAAGCCACA GGACTTCCTG CGGCTTCAGC 
     2521 AAGTCTCCAG ACCCACCTTC TTTGCCCGGA AGTTTGAGTC GACTGTGAAC CAGGAAGTCC 
     2581 TGGAAATTTT GGACTTCCAC CTGTATGGCA GCTACCCACC CGGCACCCCA GCCCTCAAGG 
     2641 CCTACTGGGA GAACATCTAC GACATGGCCG ATGGCCCTAG TGGACTCAGC GATGTCCTAC 
     2701 TCACTGCTTA CACAGCCTTT GCCCGTATCA GTCTGCGTCA TGCTGCCACT GTTTCCCCAC 
     2761 TGGCCACTGC AGTCTGCAGG TTTGAGCCCA GGGGGTTGCC GTCCAGCGTG CACCTGTATT 
     2821 TCTATGACGA CCATTTCCAG GGCTACCTGG TGACGCAGGC AGTGCAGCCC TCAGCCCAGG 
     2881 GGCCAGCAGA GACACTTGAG ATGTGGCTGA TGCCCCAGAG GTTGCTGAAG CTGTTGGGGC 
     2941 ACAGTGACCA GGCCAGCCGG CTCCAGAGTC TGGAGGTTGG CACTGAGTGG GACCCCAAAG 
     3001 AACGTCTCTT CCGGAACTTT GGGGGCCTGT TGGGACCACT GGATGAACCT GTGGCCATGC 
     3061 AGCGCTGGGC CCGGGGCCCC AACCTCACAG CCACTGTGGT CTGGATTGAC CCCACCTATG 
     3121 TTGTGGCCAC ATCCTATGAC ATCACGGTAG ATGCGGACAC TGAAGTCACG CAGTACAAGC 
     3181 CCCCACTGAG CCTGCCACTG CGGCCAGGAG CCTGGACTGT TCGATTGCTT CAGTTCTGGG 
     3241 AGCCCCTGGG TGAGACCCGC TTCCTCGTGC TGCCATTGAC CTTCAACCGC AAACTACCTC 
     3301 TCAGGAAAGA TGATGCCAGC TGGCTGCATG CGGGACCACC CCACAACGAA TACATGGAAC 
     3361 AGAGTTTCCA GGGACTAAGT GGCATCCTGA ATCTGCCTCA GCCAGAGGCC GTGGAGGAGG 
     3421 CTGCCCGGCG GCACACAGAG CTCACAGGTC CTGCACTTGA GGCCTGGACA GATGGGGAAC 
     3481 TGAGCAGTTT CTGGTCTGTT GCAGGATTGT GTGCCATAGG GCCTTCTTCT TGTCCCTCCC 
     3541 TGGAGCTCTG CAGACTGACC AGCTGGAGCT CTCTGTCTCC TGACCCCAAG TCAGAGCTGG 
     3601 GGCCTGTCAA AGCTGACGGA CGACTCAGGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3661 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3721 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3781 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3841 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3901 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3961 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4021 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4081 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4141 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4201 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4261 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4321 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4381 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4441 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4501 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4561 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4621 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4681 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4741 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4801 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4861 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4921 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4981 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5041 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5101 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5161 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5221 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5281 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5341 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5401 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5461 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5521 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5581 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5641 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5701 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5761 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5821 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5881 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5941 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6001 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6061 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6121 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6181 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6241 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6301 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6361 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6421 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6481 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6541 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6601 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6661 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6721 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6781 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6841 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6901 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6961 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7021 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7081 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7141 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7201 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7261 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7321 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7381 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7441 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//