LOCUS       RR206853                8025 bp    DNA     circular  SYN 
DEFINITION  Ephb3 (Myc-DDK-tagged ORF) - Rat Eph receptor B3 (Ephb3), (10 ug)
ACCESSION   NM_001105868
VERSION     NM_001105868.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4182..4273 
                     /label="Myc-DDK"  
     misc_feature    4303..4888 
                     /label="PolyA signal"  
     misc_feature    4360..4379 
                     /label="Restriction Enzyme"  
     misc_feature    5164..5836 
                     /label="ColE1"  
     misc_feature    6266..7060 
                     /label="Kan/Neo"  
     misc_feature    7095..7397 
                     /label="SV40 ori"  
     misc_feature    7483..7960 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4164..4170 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4164 
                     /label="Ephb3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGGAGCC CGCCCGCCGC CGGGGCTTCT GCCGCTGCTC GCTCCGCTGC 
     1081 TGCTGCCGCT GCTGCTGCCC GCCGGCTGCC GGGCGCTGGA AGAGACTCTC ATGGACACGA 
     1141 AATGGGTGAC GTCCGAGCTG GCATGGACAT CTCATCCAGA GAGTGGGTGG GAAGAAGTGA 
     1201 GCGGCTACGA TGAAGCCATG AATCCTATCC GCACGTATCA GGTGTGTAAC GTGCGCGAGT 
     1261 CCAGCCAGAA CAACTGGCTG CGGACCGGTT TCATCTGGCG GCGGGAAGTC CAGCGCGTCT 
     1321 ACGTGGAGCT GAAGTTTACC GTGAGAGATT GCAACAGCAT CCCCAACATC CCTGGCTCCT 
     1381 GCAAGGAAAC CTTCAACCTT TTTTACTACG AGGCTGATAG CGATGTGGCG TCAGCCTCCT 
     1441 CTCCCTTCTG GATGGAGAAC CCCTACGTGA AAGTGGACAC CATTGCGCCA GATGAGAGCT 
     1501 TCTCGCGGCT AGACGCTGGG CGCGTTAACA CCAAAGTGCG CAGCTTCGGG CCGCTTTCCA 
     1561 AAGCCGGCTT CTACTTGGCC TTCCAGGACC AGGGTGCCTG CATGTCACTC ATCTCTGTGC 
     1621 GCGCCTTCTA CAAGAAGTGT GCATCCACCA CTGCAGGCTT CGCACTCTTC CCCGAGACCC 
     1681 TCACGGGGGC TGAGCCCACT TCGCTGGTCA TTGCCCCTGG CACCTGCATC GCTAACGCTG 
     1741 TGGAGGTGTC TGTACCGCTC AAGCTCTACT GCAATGGCGA CGGGGAGTGG ATGGTGCCCG 
     1801 TTGGTGCCTG CACCTGCGCT ACTGGCCATG AGCCAGCCGC CAAGGAGACC CAGTGCCGCG 
     1861 CCTGTCCCCC TGGGAGCTAC AAGGCAAAGC AAGGAGAGGG GCCCTGCCTC CCCTGTCCCC 
     1921 CCAATAGCCG CACCACCTCG CCGGCTGCCA GCATCTGCAC CTGTCACAAT AATTTCTACC 
     1981 GCGCAGACTC AGACACAGCG GACAGCGCCT GCACCACGGT GCCGTCTCCC CCCCGGGGTG 
     2041 TGATCTCCAA TGTGAATGAG ACCTCGCTGA TCCTCGAGTG GAGTGAGCCC CGGGACCTTG 
     2101 GCGGACGAGA TGACCTCCTT TATAATGTTA TCTGTAAGAA GTGCCGTGGC AGCTCTGGGG 
     2161 CTGGAGGTCC GGCGACCTGT TCACGCTGTG ATGACAACGT GGAGTTCGAG CCCCGACAGC 
     2221 TGGGCCTGAC CGAGCGCCGG GTCCACATCA GCCACCTGTT GGCCCACACC CGCTACACCT 
     2281 TTGAGGTGCA GGCTGTCAAC GGCGTCTCTG GCAAAAGCCC TTTGCCGCCC CGCTATGCAG 
     2341 CTGTGAATAT CACCACCAAC CAGGCCGCCC CATCAGAAGT GCCTACGCTC CACTTGCACA 
     2401 GCAGTTCAGG GAGCAGCCTG ACCCTGTCCT GGGCACCCCC GGAGCGGCCT AACGGAGTCA 
     2461 TCTTGGACTA TGAGATGAAG TACTTTGAGA AGAGTAAAGG CATCGCCTCC ACTGTCACCA 
     2521 GCCAGAAGAA CTCTGTACAA CTGGACGGAC TGCAGCCCGA CGCCCGCTAT GTAGTTCAGG 
     2581 TCCGGGCTCG CACAGTAGCA GGTTACGGAC AGTATAGCCG CCCAGCTGAG TTTGAGACCA 
     2641 CGAGTGAAAG AGGCTCAGGG GCCCAGCAGC TTCAAGAGCA GCTTCCCCTA ATTGTGGGAT 
     2701 CCACCGTAGC TGGCTTTGTC TTCATGGTGG TCGTCGTGGT CATTGCTCTT GTCTGCCTCA 
     2761 GGAAGCAGCG CCAGGGCCCT GATGCAGAAT ACACGGAGAA GTTGCAGCAA TACGTTGCCC 
     2821 CCAGGATGAA AGTTTACATT GACCCCTTTA CCTACGAGGA TCCCAATGAG GCCGTCCGAG 
     2881 AGTTCGCCAA GGAGATCGAT GTGTCCTGCG TCAAGATCGA GGAGGTGATT GGAGCTGGGG 
     2941 AGTTTGGGGA AGTGTGCCGG GGTCGGCTGA AACTGCCCGG CCGCCGGGAG GTGTTCGTGG 
     3001 CCATCAAGAC ACTGAAGGTG GGATACACGG AGAGGCAGCG GCGGGACTTC CTGAGTGAGG 
     3061 CTTCCATCAT GGGTCAATTT GACCATCCAA ATATAATCCG TCTAGAGGGC GTGGTCACCA 
     3121 AAAGTCGTCC AGTCATGATC CTCACTGAGT TCATGGAGAA CTGTGCCCTG GACTCCTTCC 
     3181 TACGGCTCAA TGACGGGCAG TTCACAGTCA TCCAGCTTGT GGGCATGTTG CGTGGCATTG 
     3241 CTGCCGGCAT GAAGTACTTG TCTGAGATGA ACTACGTGCA CCGTGACCTC GCTGCCCGCA 
     3301 ACATCCTTGT CAACAGTAAC TTGGTCTGCA AAGTATCTGA CTTTGGGCTC TCCCGCTTCC 
     3361 TGGAGGACGA CCCCTCAGAC CCCACCTACA CCAGCTCCCT GGGTGGGAAG ATCCCTATCC 
     3421 GTTGGACCGC CCCAGAGGCC ATAGCCTATC GGAAGTTCAC GTCTGCCAGC GATGTCTGGA 
     3481 GCTACGGGAT CGTCATGTGG GAGGTCATGA GCTACGGAGA GCGACCATAC TGGGACATGA 
     3541 GCAACCAGGA TGTCATCAAT GCCGTAGAGC AAGACTATCG GTTACCACCC CCCATGGACT 
     3601 GCCCCACGGC GCTGCACCAG CTCATGCTGG ACTGTTGGGT GCGGGACCGG AACCTCAGGC 
     3661 CCAAGTTCTC CCAAATCGTC AACACGCTAG ACAAGCTTAT CCGCAATGCT GCCAGCCTCA 
     3721 AGGTCATCGC CAGTGCCCCA TCTGGCATGT CCCAGCCCCT CCTAGACCGC ACGGTCCCAG 
     3781 ATTATACGAC CTTCACGACG GTGGGCGACT GGCTAGATGC CATCAAGATG GGGAGGTATA 
     3841 AAGAGAGCTT CGTCGGTGCG GGTTTTGCCT CCTTTGACCT GGTGGCCCAG ATGACTGCAG 
     3901 AAGATCTGCT AAGGATCGGG GTCACTTTGG CCGGCCACCA GAAGAAGATC CTCAGCAGTA 
     3961 TCCAGGACAT GCGGCTGCAG ATGAACCAGA CACTGCCCGT GCAGGAAGTG TGCCCCAAAC 
     4021 CTCTTCATAT TGAAGATGGA TTAGAAGAGG GGGTGATATC CCCTCCCCAG ATGCCTCAGG 
     4081 GCCCAGGCCT GCCTGCTCTC CAGTCGGGGA TCTTCACAAC TCAGATTTGG TTGTGCTTCA 
     4141 GTAGTGGAGG TCCTGGTAGG GTCGGGTGGG GAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4201 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4261 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4321 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4381 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4441 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4501 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4561 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4621 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4681 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4741 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4801 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4861 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4921 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4981 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5041 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5101 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5161 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5221 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5281 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5341 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5401 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5461 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5521 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5581 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5641 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5701 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5761 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5821 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5881 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5941 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6001 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6061 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6121 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6181 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6241 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6301 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6361 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6421 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6481 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6541 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6601 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6661 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6721 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6781 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6841 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6901 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6961 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7021 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7081 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7141 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7201 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7261 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7321 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7381 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7441 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7501 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7561 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7621 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7681 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7741 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7801 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7861 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7921 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7981 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//