LOCUS       RR206831                7347 bp    DNA     circular  SYN 
DEFINITION  Fes (Myc-DDK-tagged ORF) - Rat feline sarcoma oncogene (Fes), (10 ug)
ACCESSION   NM_001108488
VERSION     NM_001108488.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3504..3595 
                     /label="Myc-DDK"  
     misc_feature    3625..4210 
                     /label="PolyA signal"  
     misc_feature    3682..3701 
                     /label="Restriction Enzyme"  
     misc_feature    4486..5158 
                     /label="ColE1"  
     misc_feature    5588..6382 
                     /label="Kan/Neo"  
     misc_feature    6417..6719 
                     /label="SV40 ori"  
     misc_feature    6805..7282 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3486..3492 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3486 
                     /label="Fes"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTTCTCT TCAGAGCTGT GCAGCCCCCA GGGCCACGGG GCAGTGCAAC 
     1081 AGATGCAAGA AGCTGAGCTT CGTTTATTGG AAGGGATGAG GAAGTGGATG GCGCAGAGGG 
     1141 TCAAGAGTGA CCGGGAATAT GCAGGATTGC TTCACCACAT GTCCTTGCAA GACAGCGGGG 
     1201 GCCAGAGCTG GAGCAGTGGC CCTGACAGCC CTGTCAGCCA GTCCTGGGCA GAGATAACAA 
     1261 GCCAGACGGA GAACTTGAGC CGGGTACTGC GGCAGCATGC AGAAGATCTG AACTCAGGGC 
     1321 CCCTGAGCAA GCTGAGCGTG CTGATCCGGG AGCGGCAGCA CCTGAGAAAG ACTTACAACG 
     1381 AGCAGTGGCA GCAGCTGCAG CAGGAGCTCA CCAAGACCCA CAGCCAGGAC ATTGAGAAGC 
     1441 TAAAGAGCCA GTACCGGACC CTGACACGAG ATAGTGCCCA GGCCAGGCGC AAGTACCAGG 
     1501 AAGCCAGCAA AGACAAGGAT CGCGACAAGG CCAAAGACAA GTATGTACGA AGCCTGTGGA 
     1561 AGCTCTTTGC CCACCACAAC CGCTACGTAC TGGGTGTGAG GGCCGCGCAG CTGCACCACC 
     1621 AGCACTACCA CCAATTCATG CTGCCTGGCC TGCTGCAGTC ACTACAGGAC TTGCACGAAG 
     1681 AGATGGCAGG CATCCTGAAG GACATCCTGC AGGAATACCT AGAGATCAGC AGTCTGGTGC 
     1741 AGGACGATGT GGCATCCATT CACCGTGAGC TGGCTGCAGC TGCTGCTCGC ATCCAGCCTG 
     1801 AGTTTGAGTA CCTAGGCTTC CTGCGACAGT ACGGATCTGC CCCTGATGTC CCACCTTGTG 
     1861 TCACTTTTGA TGTGTCACTT CTTGAAGAGG GCGAACAGCT GGAACCTGGG GACCTGCAGC 
     1921 TGAACGAGCT GACCTTGGAG AGCGTGCAGC ACACGCTGAC CTCTGTGACA GACGAACTGG 
     1981 CTGTGGCCAC CAAAGAGGTG CTGAGCCGGC AGGAGATGGT CAGTCAGCTG CAGCGGGAGC 
     2041 TCCAGTGCGA GGAGCAGAAC ACCCACCCCC GAGAACGGGT GCAGCTTCTG GGCAAGAGGC 
     2101 AGGTGCTGCA AGAGGCGATG CAGGGGTTGC AGGTAGCACT GTGCAATCAG GACAAGCTTC 
     2161 AGGCTCAGCA GGAACTTCTA CAGAGCAAGA TGGAGCAGCT CGGGACAGGC GAGCCCCCAG 
     2221 CCGTACCACT CCTTCAGGAT GACCGTCATT CCACCTCTTC CACGGAGCAG GAGCGAGATG 
     2281 GGGGAAGGAC ACCCACCCTG GAGATCATTA AAAGCCACTT CTCTGGAATC TTCCGCCCCA 
     2341 AGTTCTCCAT CCCTCCTCCG CTGCAGCTCG TTCCAGAGGT GCAGAAGCCC CTCCACGAGC 
     2401 AGCTGTGGTA CCATGGGGCT ATCCCTCGGG CAGAGGTAGC TGAGCTGCTA ACACACTCCG 
     2461 GGGACTTCCT GGTTCGGGAG AGCCAGGGCA AGCAGGAGTA TGTTCTGTCT GTGCTGTGGG 
     2521 ATGGCCAGCC CCGACACTTC ATCATCCAAT CCTCAGATAA CCTGTACCGG CTGGAAGGGG 
     2581 ACGGCTTTCC CAGCATCCCT TTGCTCATCA CTCACCTGCT GTCCTCCCAG CAGCCCCTTA 
     2641 CAAAGAAGAG TGGTGTTGTC TTGTTCAGGG CGGTACCCAA GGACAAGTGG GTACTGAAGC 
     2701 ATGAGGATCT GGTGCTGGGA GAGCAGATCG GAAGGGGGAA CTTCGGAGAG GTGTTTAGTG 
     2761 GACGTCTTCG TGCAGACAAC ACCCCGGTGG CTGTGAAGTC TTGTCGAGAG ACGCTCCCAC 
     2821 CTGACCTCAA GGCCAAGTTT CTGCAAGAAG CGAGGATCCT GAAACAGTAC AACCACCCCA 
     2881 ACATTGTACG TCTCATCGGG GTCTGCACAC AGAAACAGCC CATCTACATT GTCATGGAGC 
     2941 TGGTTCAAGG GGGCGACTTT CTCACCTTTC TGCGGACAGA GGGAGCCCGC CTGCGGGTGA 
     3001 AGACCCTGCT GCAGATGGTA GGGGATGCAG CTGCTGGCAT GGAGTACTTG GAAAGCAAGT 
     3061 GCTGTATCCA CCGGGATCTG GCTGCTCGCA ACTGCCTGGT GACAGAGAAG AATGTTCTGA 
     3121 AGATCAGTGA CTTCGGGATG TCTCGAGAAG AGGCTGACGG GATCTACGCG GCCTCAGCCG 
     3181 GCCTCAGACA AGTCCCTGTT AAGTGGACTG CCCCTGAAGC TCTTAATTAT GGACGCTACT 
     3241 CCTCAGAAAG TGACGTGTGG AGCTTTGGCA TTTTGCTGTG GGAGACCTTC AGCCTAGGGG 
     3301 CCTCACCTTA TCCCAACCTC ACCAATCAGC AGACACGGGA GTTTGTCGAA AAGGGGCATC 
     3361 GTCTGCCTTG CCCAGAGCTG TGCCCTGATG CAGTCTTCAG GCTCATGGAG CAGTGCTGGG 
     3421 CCTATGAGCC GGGGCAGAGG CCTAGCTTCA GCACCATCTG CCAGGAGCTA CAGAGTATCC 
     3481 GAAAGCGGCA TCGGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3541 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3601 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3661 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3721 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3781 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3841 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3901 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3961 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4021 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4081 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4141 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4201 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4261 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4321 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4381 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4441 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4501 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4561 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4621 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4681 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4741 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4801 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4861 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4921 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4981 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5041 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5101 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5161 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5221 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5281 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5341 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5401 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5461 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5521 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5581 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5641 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5701 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5761 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5821 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5881 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5941 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6001 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6061 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6121 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6181 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6241 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6301 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6361 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6421 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6481 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6541 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6601 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6661 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6721 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6781 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6841 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6901 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6961 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7021 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7081 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7141 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7201 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7261 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7321 ATTTAACAAA AATTTAACGC GAATTTT
//