LOCUS       RR206812                7002 bp    DNA     circular  SYN 
DEFINITION  Pja2 (Myc-DDK-tagged ORF) - Rat praja 2, RING-H2 motif containing (Pja2), (10 ug)
ACCESSION   NM_138896
VERSION     NM_138896.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3159..3250 
                     /label="Myc-DDK"  
     misc_feature    3280..3865 
                     /label="PolyA signal"  
     misc_feature    3337..3356 
                     /label="Restriction Enzyme"  
     misc_feature    4141..4813 
                     /label="ColE1"  
     misc_feature    5243..6037 
                     /label="Kan/Neo"  
     misc_feature    6072..6374 
                     /label="SV40 ori"  
     misc_feature    6460..6937 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3141..3147 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3141 
                     /label="Pja2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCACAGTAT ACAGAAAAAG AACCATCAGT AATGGATCAA GACTCCAGCA 
     1081 AGGCTGCGTG GCCTAGAGCA GCAGGAGGAT ACCAGACGAT TACAGGCAGG AGGTACGGAA 
     1141 GGAGACATGC GTATGTCAGT TTTAAGCCAT GTATGACCAG GCATGAACGG AGCTTGGGTC 
     1201 GGGCTGGCGA TGACTATGAA GTTCTGGAAC TGGATGACGT TGCCAAGGAA AACACCGCAG 
     1261 GTTCCAGTTC ATTGGATCAA GTCCATCCTT CTTTACCCAG TGAAACTACA GTTGAAAAAA 
     1321 GTGAAACAGA AATTCCTACT TGTGGTCCAG CACTGAATCA AAGCACGGAG AGCAACCCAT 
     1381 CCGTTGCCAC AGTGTGTCAT AGTGAGGAAG TCAGGGAGAC CTTAGACAGC AGTACGAATC 
     1441 TTCAGAATCA CGCTGAGAGA GAGTGTACGC CAGCAGTTTG TAATGCCTCA AGTGTCCAGA 
     1501 ATGGAATTGT GTTGGTTCAT ACTGACTCTT ATGATCCAGA CAGCAAGCAT GATGAGAATG 
     1561 ACTCTCTTCA ACTTTGTGCC CAAGCTGTGG AAGGTGGTAG ACGTCAGAAG GTATTAGGCA 
     1621 ATGCAGTCTT TGAGCTGGAA AATGGAGAGG TAGAGAGATA TGCTGATCTG TGTCCCTCAG 
     1681 TTCCCTCTCT CAGTGGTGAA ATAAGGGAGG AGTCTGAAGA GCTAGGTTCA GCACTCTTAG 
     1741 AGAAAAATTC TGCTGGCGAT GCGGAGGCTG TCCATCAGGA TGGGCAGGAA TTTCAGAGGT 
     1801 CTTCTGAAGA CGGCATTGTT AGAAAGAGGC GACAAGATGA TACCGATCAG GGAAGACAGA 
     1861 CAGAAAATTC AACTGAAGAT GCAGACTGTG TTCCAGGCCA TGTTGAACAA AATACTAGTG 
     1921 AGAGAGCCAA TCACCATGGA AGTTCTCCTG AACAGGTAGT GAGGCCCAAA GTTAGAAAAG 
     1981 TGATCAGTTC AAGCCAGGTG GACCAAGAGA GCGGTTTTAA TAGGCACGAG GCTAAGCAAA 
     2041 GAAGTGTTCA GAGGTGGAGA GAGGCTCTGG AAGTTGAGGA ATGCAGTTCA GATGACCCTA 
     2101 TAATCAAGTG TGACGATTAT GATGGAGACC ATGACTGCAT GTTCCTAACC CCATCCTACT 
     2161 CAAGAGTTAC GCCAAGGGAA GCAGAACGTC ACCGTGCGAC AGCAGAAAAT GGAGCCACAG 
     2221 CTTCAGGAAG GCAAGAGGCT CGGGAAAATG CCTTTTGGAA TGCCTGTGGA GAGTATTACC 
     2281 AGCTCTTTGA CAAAGACGAA GACAGTTCAG AGTGCAGTGA TGGGGAATGG TCTGCTTCTC 
     2341 TGCCTCACCG ATTTTCTGGC ACAGAAAAAG ACCAGTCCTC AAGCGATGAA AGCTGGGAAA 
     2401 CTCTGCCAGG AAAAGATGAG AATGAACCTG AGCTACAGAG TGATAGTAGT GGCCCTGAGG 
     2461 AAGAAAACCA AGAATTGTCT CTTCAGGAGG GAGAACAGAC GTCCTTGGAG GAGGGGGAGA 
     2521 TTCCCTGGTT ACAGTACAAT GAGGTCAATG AGAGCAGCAG CGATGAAGGG AACGAGCCTG 
     2581 CCAATGAGTT TGCACAACCA GAAGCTTTCA TGTTGGATGG GAACAACAAC CTGGAGGACG 
     2641 ACTCGAGCGT GAGTGAAGAC CTGGATGTGG ACTGGAGCCT ATTTGATGGT TTTGCGGATG 
     2701 GACTTGGTGT TGCTGAAGCT ATTTCCTACG TGGATCCTCA GTTCCTCACC TACATGGCAC 
     2761 TAGAAGAACG CTTAGCCCAG GCTATGGAGA CTGCACTGGC ACACTTAGAG TCTCTTGCTG 
     2821 TAGACGTTGA GGTGGCTAAC CCACCTGCCA GTAAGGAAAG CATCGACGGA CTTCCAGAAA 
     2881 CCCTTGTCCT AGAAGATCAC ACCGCTATTG GTCAGGAGCA GTGCTGTCCC ATCTGCTGCA 
     2941 GTGAGTACAT TAAGGATGAC ATCGCAACAG AGCTGCCCTG CCATCACTTC TTTCATAAGC 
     3001 CTTGTGTCTC CATTTGGCTA CAGAAGTCCG GAACGTGCCC TGTGTGCCGC CGCCACTTCC 
     3061 CACCTGCAGT GATTGACGCA TCTGCAGCTG CTTCCTCTGA ACCAGACCTT GATGCCTCGC 
     3121 CTGCAAACGA CAATGCTGAG GAAGCCCCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3181 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3241 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3301 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3361 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3421 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3481 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3541 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3601 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3661 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3721 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3781 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3841 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3901 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3961 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4021 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4081 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4141 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4201 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4261 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4321 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4381 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4441 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4501 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4561 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4621 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4681 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4741 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4801 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4861 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4921 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4981 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5041 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5101 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5161 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5221 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5281 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5341 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5401 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5461 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5521 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5581 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5641 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5701 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5761 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5821 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5881 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5941 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6001 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6061 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6121 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6181 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6241 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6301 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6361 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6421 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6481 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6541 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6601 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6661 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6721 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6781 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6841 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6901 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6961 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//