LOCUS       RR206808                7260 bp    DNA     circular  SYN 
DEFINITION  Mark1 (Myc-DDK-tagged ORF) - Rat MAP/microtubule affinity-regulating kinase 1 (Mark1), (10 ug)
ACCESSION   NM_053947
VERSION     NM_053947.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3417..3508 
                     /label="Myc-DDK"  
     misc_feature    3538..4123 
                     /label="PolyA signal"  
     misc_feature    3595..3614 
                     /label="Restriction Enzyme"  
     misc_feature    4399..5071 
                     /label="ColE1"  
     misc_feature    5501..6295 
                     /label="Kan/Neo"  
     misc_feature    6330..6632 
                     /label="SV40 ori"  
     misc_feature    6718..7195 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3399..3405 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3399 
                     /label="Mark1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCGCGG ACGCCATTGC CCACGGTGAA CGAGCGGGAC ACGGAGAACC 
     1081 ATACATCTGT GGATGGATAT ACTGAAACGC ATATCCCGCC TACCAAATCA AGTAGCAGAC 
     1141 AAAACATTCC CCGGTGTAGA AACTCCATTA CGTCAGCGAC AGATGAACAG CCTCACATCG 
     1201 GAAATTACCG TTTACAGAAA ACAATAGGGA AGGGGAATTT TGCCAAAGTG AAATTGGCAA 
     1261 GGCATGTCCT AACAGGTAGA GAGGTAGCTG TGAAAATAAT AGACAAAACT CAGCTAAACC 
     1321 CTACCAGTCT ACAAAAGTTG TTCCGAGAAG TACGAATAAT GAAGATACTG AACCATCCTA 
     1381 ACATAGTGAA ACTGTTTGAA GTTATTGAAA CAGAGAAGAC GCTCTACCTG GTCATGGAAT 
     1441 ACGCAAGTGG GGGTGAAGTC TTTGATTACT TAGTTGCCCA CGGAAGAATG AAAGAGAAAG 
     1501 AGGCCCGGGC CAAATTCAGG CAGATTGTAT CTGCTGTACA GTATTGTCAT CAAAAATGCA 
     1561 TTGTCCACCG TGATCTTAAG GCTGAAAACC TTCTCCTTGA TGCTGATATG AATATTAAAA 
     1621 TTGCTGACTT TGGTTTTAGC AATGAATTCA CAGTGGGGAA CAAATTGGAC ACATTCTGTG 
     1681 GAAGCCCGCC TTATGCTGCC CCTGAGCTTT TCCAAGGCAA GAAGTACGAC GGGCCGGAAG 
     1741 TGGATGTGTG GAGTCTAGGC GTCATTCTCT ACACGCTGGT CAGTGGCTCC CTGCCTTTCG 
     1801 ATGGCCAGAA TCTAAAGGAG CTCCGGGAGC GGGTCCTGCG AGGGAAGTAC CGTGTTCCTT 
     1861 TCTACATGTC CACGGACTGC GAGAACCTCC TGAAGAAACT GCTAGTGCTG AACCCCATAA 
     1921 AGAGGGGCAG CCTGGAGCAA ATAATGAAAG ACCGATGGAT GAACGTTGGT CATGAAGAGG 
     1981 AAGAGCTAAA ACCATATTCT GAGCCTGAGC TTGATCTCAA TGATGCAAAA AGAATAGACA 
     2041 TTATGGTCAC CATGGGTTTT GCACGAGATG AAATCAATGA TGCCTTAGTA AGCCAGAAAT 
     2101 ATGATGAGGT TATGGCTACT TATATTCTTC TAGGTAGAAA ACCACCTGAG TTCGAAGGTG 
     2161 GTGAGTCACT GTCCAGTGGA AACCTTTGTC AGCGATCCCG GCCGAGCAGT GACCTGAACA 
     2221 ACAGCACGCT TCAGTCACCT GCTCACCTGA AGGTCCAGCG AAGCATCTCA GCCAACCAGA 
     2281 AGCAGAGGCG CTTCAGTGAC CATGCCGGTC CCTCCATCCC CCCTGCTGTC TCATACACCA 
     2341 AAAGGCCTCA GGCCAACAGC GTGGAGAGTG AACAGAAAGA GGAGTGGGAC AAAGACACAG 
     2401 CCCGCAGACT TGGCAGCACC ACCGTGGGCT CGAAGAGCGA AGTCACAGCC AGCCCCTTGG 
     2461 TGGGGCCAGA CAGGAAAAAG TCCTCAGCTG GCCCAAGTAA CAACGTATAT TCTGGCGGTA 
     2521 GCATGACGAG GAGAAATACA TATGTGTGTG AGAGGAGCAC GGACCGGTAC GCTGCCTTGC 
     2581 AGAATGGAAG AGACAGCAGC CTTACAGAGA TGTCTGCCAG CAGCATGTCC TCCACAGGTT 
     2641 CCACTGTGGC CTCTGCTGGC CCCTCAGCAC GTCCCCGCCA CCAGAAGTCT ATGTCAACCT 
     2701 CTGGCCACCC TATTAAAGTT ACACTGCCAA CCATTAAAGA TGGCTCTGAA GCTTATCGGC 
     2761 CTGGTACAGC TCAGAGAGTG CCTGCTGCTT CCCCGTCTGC TCACAGCATT AGTGCCAGTA 
     2821 CTCCTGACAG GACCCGCTTC CCCCGAGGGA GCTCCAGCCG AAGCACTTTC CACGGGGAGC 
     2881 AGCTCCGAGA GCGGCGCAGT GCTGCTTATA GCGGACCACC TGCCTCCCCG TCCCATGACA 
     2941 CAGCTGCACT TGCACATGCC AGAAGGGGGA CGTCAACTGG GATAATCAGC AAAATCACCT 
     3001 CCAAGTTCGT CCGCAGGGAC CCAAGTGAAG GCGAAGCCAG TGGCAGAACC GACACAGCCA 
     3061 GAGGTTCATC GGGGGAGCCG AAAGACAAGG AAGAGGGTAA GGAGGCCAAG CCGAGGTCGC 
     3121 TGCGCTTCAC GTGGAGCATG AAGACCACTA GTTCAATGGA CCCCAATGAC ATGGTGAGAG 
     3181 AGATCCGCAA GGTGCTGGAT GCCAACACCT GTGACTATGA ACAGAGAGAG AGGTTCCTGC 
     3241 TCTTCTGCGT TCACGGAGAC GCTCGGCAGG ACAGCCTCGT ACAGTGGGAG ATGGAGGTCT 
     3301 GCAAGTTGCC TCGGCTCTCA CTCAATGGGG TCCGCTTCAA GCGAATATCC GGGACATCTA 
     3361 TTGCCTTTAA GAACATTGCA TCCAAAATAG CAAATGAGCT TAAGCTGACG CGTACGCGGC 
     3421 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3481 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3541 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3601 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3661 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3721 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3781 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3841 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3901 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3961 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4021 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4081 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4141 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4201 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4261 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4321 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4381 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4441 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4501 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4561 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4621 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4681 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4741 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4801 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4861 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4921 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4981 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5041 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5101 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5161 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5221 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5281 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5341 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5401 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5461 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5521 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5581 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5641 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5701 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5761 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5821 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5881 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5941 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6001 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6061 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6121 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6181 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6241 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6301 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6361 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6421 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6481 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6541 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6601 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6661 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6721 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6781 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6841 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6901 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6961 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7021 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7081 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7141 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7201 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//