LOCUS       RR206737                9114 bp    DNA     circular  SYN 
DEFINITION  Eea1 (Myc-DDK-tagged ORF) - Rat early endosome antigen 1 (Eea1), (10 ug)
ACCESSION   NM_001108086
VERSION     NM_001108086.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5271..5362 
                     /label="Myc-DDK"  
     misc_feature    5392..5977 
                     /label="PolyA signal"  
     misc_feature    5449..5468 
                     /label="Restriction Enzyme"  
     misc_feature    6253..6925 
                     /label="ColE1"  
     misc_feature    7355..8149 
                     /label="Kan/Neo"  
     misc_feature    8184..8486 
                     /label="SV40 ori"  
     misc_feature    8572..9049 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5253..5259 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5253 
                     /label="Eea1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTCGGAGG ATCTTGCAGA GGACTCCTGG GAGAGTTGGC TCTCAAGGTT 
     1081 CTGATTTAGA TTCATCAGCA ACTCCTATAA ATACGGTGGA TGTCAATAAT GAAAGTTCTT 
     1141 CAGAGGGTTT TATATGTCCC CAGTGTATGA AATCTCTTGG CTCAGCTGAT GAACTTTTCA 
     1201 AACACTATCA GGCAGTTCAT GATGCCGGTA ATGACTCGGG GCATGGTGGA GAGGCCAGTC 
     1261 TTGCTTTGAC ACGAGATGAC ATAGCACTAC TCAGGCAAGA GGTCCAAGAC CTGCAGGCCT 
     1321 CACTTAAGGA AGAAAAATGG TACTCAGAAG AATTAAAGAA AGAATTAGAA AAATATCAAG 
     1381 GGCTACAGCA GCAAGAGGCT AAATCTGATG GATTGGTGAC TGATTCATCA GCAGAGCTAC 
     1441 AGTCTTTAGA GCAACAATTA GAAGAAGCCC AAACTGAGAA TTTTAATATT AAGCAAATGA 
     1501 AAGATCTGTT TGAACAGAAA GCAGCTCAAC TTGCTACTGA AATTGCAGAT ATAAAGTCAA 
     1561 AATATGATGA AGAAAAGGGT CTCCGAGCAG CTGCGGAACA AAAAGCGACA CATTTGACAG 
     1621 AAGAATTAAG TAAACAGACG ACTGTAATTC AAGACCTGAA AACTGAGCTG CTTCAGAGAC 
     1681 CTGGTATAGA AGATGTCGCT GTACTAAAGA AAGAACTGGT CCAAGTTCAA ACTCTAATGG 
     1741 ATAACATGAC TTTGGAGCGT GAGAGAGAAT CTGAAAAACT CAAGGATGAA TGTAAAAAGT 
     1801 TACAGTCAGA ACACGCTAAC TCGGAGGCTA CCATAAACCA GCTAAGGAGT GAACTTGCCA 
     1861 AAGGCCCCCA AGAAGTTGCA GTATATGTGC AGGAAATACA AAAACTTAAA GGTTCAGTTA 
     1921 ATGAATTAAC ACAAAAAAGC CAGACCTTGA TGGAGAAATT GCAGAAGAAA GACCTGGATT 
     1981 ACACCCACTT AGAGGAGAAG CATAACGAAG AGGCACTGAG TAGAAAGAAT ACCCAGGCAG 
     2041 CCCTCCACCA AAGGGACCTG GATTGCCAGC AGCTTCAGGC AAGGCTAACT GCATCCGAAG 
     2101 GCTCGCTACA GAGGATCCAA GGAGAGCTCA GGGAAAAGGG AGAGGCTGCT CAGAAGCTCA 
     2161 AAGAAGAACT GCGGGAAGTA GAGACCAAGC ACCAGCATCT TAAAGTGGAG TTCAAGCAGC 
     2221 TGCAGCAGCA GCGGGAAGAG AAGGAGCAGC ATGGGCTGCA GCTGCAAGGG GAAGTCAGTC 
     2281 AGTTACATAG CAAACTTCTG GAGACTGAGC GCCAACTAGG GGAAGCTCAC GGTAGGCTGA 
     2341 AGGAGCAGAG GCAACTTTCA AGTGAGAAGC TGATGGATAA GGAACAGCAA GTGGCTGACT 
     2401 TACAGCTCAA GCTCTCTCGT CTGGAAGAGC AGTTGAAGGA AAAGGTAACA AATTCCACTG 
     2461 AATTGCAACA TCAATTAGAA AAATCAAAGC AACAGCACCA AGAACAGCAA GCCCTTCAGC 
     2521 AAAGCACTAC AGCCAAACTC CGAGAAGCTC AGAATGATTT AGAACAAGTG TTACGTCAGA 
     2581 TTGGAGATAA GGACCAAAAG ATCCAGAACC TTGAAGCCTT ACTACAGAAG AGTAAAGAAA 
     2641 ATATTTCCTT ACTAGAAAAG GAAAGAGAAG ACCTTTATGC AAAAATCCAA GCCGGTGAAG 
     2701 GAGAGACTGC TGTCCTTAAC CAGTTGCAAG AGAAAAACCA TGCACTACAG CAGCAACTAA 
     2761 CTCAACTGAT GGAGAAGCTG AAGAATCAGT CGGAAAGCCA TAAACAAGCT GAGGAGAACT 
     2821 TGCATGACCA GGTACAGGAG CAGAAGGCAC ATCTTCGAGC TGCACAAGAC CGTGTTCTTT 
     2881 CTCTAGAAAC CAGTGTCAGT GAACTAAGCA GTCAGTTAAA TGAAAGCAAG GAGAAGGTTT 
     2941 CCCAGCTTGA CATACAGATT AAAGCCAAAA CTGAATTATT GCTATCAGCA GAAGCAGCAA 
     3001 AAACTGCTCA AAGAGCTGAT CTTCAAAATC ATTTGGACAC AGCTCAAAAT GCACTACAAG 
     3061 ATAAACAACA GGAGTTAAAT AAGGTCAGTG CACAGCTGGA TCAGGTCACT GCAAAGTTTC 
     3121 AGGAGAAGCA GGAGCATTGC ACTCAGCTGG AAAGCCATCT CAAGGATTAC AAGGAGAAGC 
     3181 ACCTCTCTCT AGAACAAAAA GCGGAAGACT TAGAAGGCCA GATTAAGAAA CTAGAAGCCA 
     3241 ATAATCTTGA AGTTAAAGCA AGCAGGGAGC AAGCTGTGCA GAGCCTACAG CAGCAGAGGC 
     3301 AGCTGAGCAC AGACCTAGAG CTCAGGAATG CAGAGCTCAG GAAGCAGCTT CAAGAGCAGA 
     3361 AGGAAGTAGT ATCTCATACA AAATTGGATC TACAGAATAA ATCTGAAGCC CTTGAAAATA 
     3421 TTAAACAAAC CCTCACTAAG AAAGAAGAAG AAAAAGTTGT CTTGAAACAG GAATTTGAAA 
     3481 AGTTAAGTCA AGATTCAGAG GCGCAGCACA AGGAGCTGAG TGACAGGATG CAAGCTGCGA 
     3541 TCACAGAGCT GATGGTGATG ACAGCACAGA AGGACGCTCT CCTGGCTGAG CTCTCCACAA 
     3601 CAAAAGGGAA ACTGTCAAAA GTTTCAGATT CTTTACAAAC CTCCAGGAGC GAATTTGAGA 
     3661 AAGAGAGTCA GAAAGGAAAG GCTGCTGTAC TAGACTTGGA AAAAGCTTGT AAAGAATTAA 
     3721 AGCATCAGCT TCAACAACAA ACAGAAAGTG CACTGAAGGA GCAGAGTGAA CTGAAAAAGT 
     3781 CACTGGAGAA GGAGAGAGAG ACTTCTCAAC AGTTGATGAT AGAACTCAAT TCAGTCAAGG 
     3841 GAGAAGTCAC ACAGGCTCAA AATACATTAA AGCAGAAGGA GAAAGAAGAG CAGCAGCTTC 
     3901 AGGGGAACAT AAATCAGCTG AAGCAAGCAA CTGAGCAAAA GAAAAAGCAA ATGGAAGCTC 
     3961 TTCAAGGAGA GCTTAAAAAC GTTACTTCAC AAAAGGCCCA GCTGGAGAGT AAACTGCAGC 
     4021 AGCAGGCGGC ACAGGCAGCC CAGGAACTTG CTGTGGAGAA AGGAAAGCTG TCAGCACTGC 
     4081 AGAGCACCTA TGACAAGTGC CAGGCCGACC TGCAACAGCT GCAGTCTGAC CTCTATGGCA 
     4141 AAGAGTCTGA ACTTCTAGCC ACAAGGCAAG ACCTTAAGTG TGTAGAAGAG AAGCTTGCTC 
     4201 TGGCACAAGA AGACCTGATT TCAAACAGAA ATCAAATTGG AAACCAAAAT AAATCAATTC 
     4261 AAGAACTGCA GGCTGCCAAA GCTGCTTTGG AGCAGGACTT AGCAAAGAAG GAGGAGGTGC 
     4321 TCAAGGAGCA GAGTAAAGCA CTGCAGGATG CGCAGAGAGA AAAGTCAGTG AAGGAAAAAG 
     4381 AACTAGTAAC TGAAAAGTCT AAATTGGCAG AGATGGAGGA AATTAAATGT AGACAAGAAA 
     4441 AAGAAATCGC TAAACTCAAA GAAGAACTCA AGTCCCACAA ACAAGAAAGC ATAAAGGAAG 
     4501 TAACAAATCT CAAGGATGCC AAACAACTTT TGATTCAACA GAAATTAGAA CTCCAAGGCA 
     4561 AAGTGGACTC ACTGAAGGCA GCTCTTGAGC AGGAGAAGGA GCGTCAGCAG CTAATGCGGG 
     4621 AGCAGATGAG GAAGGAAGAA GAGAAGCGGA AGGAAGAATT TTCTGAGAAG GAAGCAAAAC 
     4681 TGCATTCTGA AATAAAAGAA AAGGAAGCAG GAGTGAAGAA ACATGAAGAA AATGAGGCTA 
     4741 AGCTTACCAT GCAGGTCACA ACATTAAATG AAAACTTGGG CACTGTGAAG AAGGAGTGGC 
     4801 AGTCTAGTCA ACGGAGAGTT AGTGAGCTTG AGAAGCAGAC GGATGACTTA CGGGGTGAAA 
     4861 TCGCTGTCTT AGAAGCAACT GTTCAGAATA ATCAGGATGA AAGAAGAGCA CTGCTAGAGA 
     4921 GATGTCTTAA AGGAGAAGGT GAAATAGAAA AGCTTCAGAC CAAAGTCCTA GAGTTGCAAA 
     4981 GAAAGCTGGA TAACACAACT GCAGCTGTGC AGGAGCTGGG CAGAGAAAAC CAGTCCCTAC 
     5041 AGATCAAACA CACACAAGCC CTGAACAGAA AGTGGGCAGA AGACAACGAG GTACAGAACT 
     5101 GCATGTCCTG TGGGAAGTGC TTCTCAGTCA CCGTGCGGCG GCACCACTGC CGACAGTGTG 
     5161 GAAATATCTT CTGTGCTGAG TGTTCAGCCA AAAACGCCTT AACTCCTTCT TCGAAGAAGC 
     5221 CTGTTCGTGT CTGTGATGCA TGTTTCAATG ATTTGCAAGG AACGCGTACG CGGCCGCTCG 
     5281 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5341 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5401 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5461 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5521 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5581 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5641 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5701 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5761 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5821 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5881 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5941 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6001 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6061 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6121 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6181 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6241 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6301 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6361 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6421 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6481 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6541 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6601 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6661 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6721 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6781 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6841 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6901 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6961 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7021 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7081 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7141 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7201 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7261 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7321 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7381 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7441 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7501 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7561 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7621 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7681 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7741 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7801 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7861 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7921 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7981 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8041 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8101 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8161 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8221 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8281 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8341 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8401 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8461 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8521 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8581 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8641 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8701 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8761 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8821 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8881 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8941 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9001 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9061 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//